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Biochar and zeolite are widely used in the remediation of soil contaminated with toxic heavy metals. However, the interaction of these amendments and their effects on grass productivity have not been comprehensively summarized. The aim of this study was to investigate the biological effects of zeolite and biochar used as soil amendments in the process of remediating soil contaminated with Cd, Pb and Zn. In a pot experiment, the following treatments were applied: zeolite, biochars produced at temperatures of 350 °C and 550 °C, mixtures of biochars and zeolite, and a control treatment without any amendments. The soil amendments were tested on two grass species: tall fescue ( Festuca arundinacea Schreb.) and cocksfoot ( Dactylis glomerata L.). The root morphometric parameters and aboveground production were determined in 2017 and 2018. Higher biomass production was observed in the tested grasses in the treatments with zeolite alone (0.229 kg DM m −2 ) or mixed with the biochars (0.239 kg DM m −2 ) than in control treatment (0.029 kg DM m −2 ). Zeolite used in contaminated soil significantly affected root biomass and root morphology parameters. Zeolite application resulted in significantly higher root biomass (2.30 mg cm −3 ) and root length (76.61 cm cm −3 ) than those in the treatments without zeolite (0.29 mg cm −3 and 6.90 cm cm −3 ). Biochar as a soil amendment did not affect most root morphometric parameters. The application of biochars only slightly reduced the root diameter of cocksfoot. The root diameter of tall fescue was similar in all treatments (0.075 mm) except the control (0.063 mm) and biochar 550 treatments (0.067 mm), in which slightly thinner roots were observed.

Combining products of natural origin with different mechanisms of action on insect herbivores may provide an alternative among methods of plant protection against pests that are less risky for the environment. The aim of the study was to evaluate the effectiveness of mixtures of Thuja occidentalis L. essential oil and diatomite (EO + DE) compared to each substance separately in reducing economically important pests such as black bean aphid (BBA) Aphis fabae Scop., Colorado potato beetle (CPB) Leptinotarsa decemlineata Say., and pea leaf weevil (PLW) Sitona lineatus L. The effects on mortality (all pests) and foraging intensity (CPB and PLW) were tested. The improvement in effectiveness using a mixture of EO + DE versus single components against BBA was dose- and the developmental stage-dependent. The effect of enhancing CPB foraging inhibition through DE addition was obtained at a concentration of 0.2% EO (both females and males of CPB) and 0.5% EO (males) in no-choice experiments. In choice experiments, mixtures EO + DE with both 0.2% and 0.5% EO concentrations resulted in a significant reduction in CPB foraging. A significant strengthening effect of EO 0.5% through the addition of DE at a dose of 10% against PLW males was observed in the no-choice experiment, while, when the beetles had a choice, the synergistic effect of a mixture of EO 0.5% and DE 10% was also apparent in females. In conclusion, the use of DE mixtures with EO from T. occidentalis appears to be a promising strategy. The results support the idea of not using doses of EO higher than 0.5%.

Despite promising results, biocomposite research still requires elaboration, particularly with regard to functional properties and applications. In this study, multilayer biocomposites based on gelatin, κ-carrageenan and carboxymethylcellulose were enriched with sage or blackberry extracts. The films were characterized based on their physicochemical traits and bioactivity for application as active packaging and environmental biodegradation. FTIR confirmed extract integration and strong matrix interactions, while UV-VIS analysis showed efficient UV blocking. Water properties remained acceptable (WVTR ≈ 550 g/m2 × d); solubility decreased for BB (41.73% vs. 53.45% control). Mechanical testing indicated a plasticizing effect: elongation increased (20.00% control; 35.35% BB; 39.29% SAGE), while tensile strength and Young’s modulus decreased. Antioxidant capacity rose (FRAP: 0.38 control, 1.97 BB, 4.48 SAGE µTrolox/mg; DPPH: 6.38% control, 85.68% BB, 78.25% SAGE; MCA: none). During refrigerated storage, antimicrobial effects were most evident on days 6–9. Lipid oxidation peaked for BB (0.92 mg MDA/kg, day 9), while pH was more stable with SAGE. Biodegradation and phytotoxicity confirmed environmental safety and compostability, with increased humic acid carbon in vermicompost. Overall, the results confirm the relevance of modifying biopolymers using green chemistry and highlight their importance for quality management, food safety and sustainable circular economy strategies.

Knowledge on the fraction of trace elements in the bottom sediments is a key to understand their mobility and ecotoxicological impact. The purpose of this study was to assess the influence of the content of organic matter fractions on the mobility and ecotoxicity of trace elements in sediments from the Rybnik reservoir. The most refractory fraction of organic matter—Cnh (non-hydrolysing carbon)—dominated in the sediments. The content of organic matter fractions are arranged in the following order: Cnh (non-hydrolysing carbon) > Cfa (fulvic acid) > Cha (humic acid) > DOC (dissolved organic carbon). On the other hand, the highest value of correlation coefficients was found for different fractions of trace elements and DOC content in the bottom sediments. A higher content of TOC in the sediments significantly increased the share of elements in the potential mobile fraction and, at the same time, decreased the binding of elements in the mobile fractions. Moreover, in sediments that contain more than 100 g/kg d.m. TOC, no and medium risk of trace element release from sediments was observed. The Cu, Cd and Ni were potentially the most toxic elements for biota in the Rybnik reservoir. However, the correlation between the content of trace elements and the response of bacteria was insignificant. These results suggested that the complexation of trace elements with organic matter makes them less toxic for Vibrio fischeri. The transformation and sources of organic matter play an important role in the behaviour of trace elements in the bottom sediments of the Rybnik reservoir.

Climate change poses significant challenges to agricultural productivity due to reduced water availability and increased temperatures. Developing innovative techniques to enhance soil water retention has emerged as a crucial strategy to mitigate these challenges. This study investigates the effects of diatomite addition type, particle size, and application rate on the physical quality of sandy soil, focusing specifically on water retention characteristics. The experiment involved three particle size fractions of diatomite mixed with additives (biochar, dolomite, and bentonite) at different rates. Soil water retention characteristics and differential porosity were evaluated. Results showed that diatomite application increased soil bulk density but improved water retention capabilities, especially when supplemented with additives. Bentonite addition with diatomite resulted in the highest available water capacity, while dolomite had minimal effect on water retention. Biochar supplementation significantly enhanced water retention characteristics, leading to higher field capacity and plant-available water capacity. The study revealed that the particle size of diatomite did not have a substantial effect on soil physical properties, except for its influence on available water capacity. Diatomite application did not lead to water repellency of soil. These findings highlight the potential of diatomite and additives to improve soil water retention, providing valuable insights for sustainable agriculture.

The foundation of microbial ecology research is Next-Generation Sequencing (NGS), which allows for reconstruction of the soil microbiome taxonomical structure and the calculation of biodiversity metrics. However, obtaining reliable data on soil biodiversity poses several challenges, with accurate primer selection being one of the most critical. While 16S rDNA primers are widely used for their ability to broadly target bacterial communities, they can introduce biases. These primers may preferentially amplify certain bacterial groups, leading to a skewed representation of the microbial diversity in soil samples. To overcome the bias, we developed a novel, Two-Step Metabarcoding (TSM) approach to obtain more accurate and detailed data on soil microbiome structure and biodiversity. The first step involved sequencing of amplicons generated using universal 16S rDNA primers, provided an initial overview of the microbial community, and allowed the identification of key taxonomical groups. In the second step, we employed sequencing of amplicons generated with taxa-specific primers designed for the most abundant phyla in the community. We used the obtained data for a more reliable reconstruction of microbiome taxonomic structure and biodiversity. This two-step approach ensures a thorough exploration of the soil microbiome and promises to enhance our understanding of soil microbial dynamics and ecology.

As natural plant growth stimulators, amino acids are widely used to improve crop yield and quality. There are numerous studies documenting the influence of amino acids on plants, which is not always positive. This study was conducted to determine the effect of soil-applied L-tryptophan (L-TRP) on the accumulation and utilization of nitrogen and sulfur by maize. The study was carried out under the conditions of a pot experiment. The experimental design included three treatments: soil without fertilization (control), soil with mineral fertilization (NPKS), and soil with mineral fertilization and L-tryptophan addition (NPKS + L-TRP). The application of tryptophan to the soil, supported by mineral fertilization, caused a significant increase in maize biomass. Although no significant differences in nitrogen and sulfur contents in maize biomass were found between treatments without and with the addition of L-tryptophan, significantly higher intakes of both elements were observed in the NPKS + L-TRP treatment. The application of L-tryptophan increased the biosynthesis of Chlorophyll a. Utilization of nitrogen and sulfur by maize in the NPKS + L-TRP treatment was more than 27% and 17% higher, respectively, compared to the NPKS treatment. Maintaining the recommended contents of individual nutrients in the rhizosphere is not a guarantee of optimal quantitative and qualitative intake of nutrients. Problems with maintaining optimal relationships between individual nutrients may be compounded by soil properties.

This research investigates the impact of compost particle size, compost additives, and application rate on the physical properties of loamy sand soil, particularly focusing on water retention characteristics. Compost, enriched with additives like zeolite, biochar, and diatomite, was applied to soil in different rates: 1%, 2%, and 4%. Compost particles were divided into three particle size classes: 0–500 µm, 500–1000 µm, and 1000–2000 µm. The study revealed significant effects of compost on soil physical quality, including bulk density, porosity, and water retention. Zeolite-enriched compost showed the most pronounced improvements in soil water retention by modifying pore diameter. However, the effectiveness of compost additives varied depending on the type and rate of application. Compost with zeolite resulted in a decrease in the volume of large soil pores with diameters of 50–500 µm and above 500 µm. This resulted in higher water retention related to mesopores. Larger compost particles (1.0–2.0 mm) exhibited superior effects on soil physical quality compared to smaller particles (<1.0 mm), although finer particles (0.5–1.0 mm) were associated with higher water repellency. Compost with diatomite resulted in higher water repellency than other compost types. The findings underscore the importance of considering compost particle size, component type, and application rate to optimize soil hydraulic characteristics, particularly in agricultural practices where water management is crucial.

Combining inorganic and organic soil amendments with mineral fertilizers is promising for soil quality enhancement in modern agronomy systems. In this research, four main organic components were used in fertilizer formulations: coconut coir, biochar, lignite, and leonardite (enriched with microelements, tryptophan, and bacterial metabolic products). The treatments were assigned to the completely randomized design with a control object, without any soil amendments, and with only mineral fertilization. Aboveground biomass and root characteristics of maize (root length density, mean root diameter, root surface area density, specific root length, root volume density, and root dry matter) and water retention and characteristics of soil pores were determined. Compared to the control, all fertilizer formulations applied deteriorated the water retention properties of the soil. The highest plant available water content value was obtained for the control without any fertilizers. The addition of organic fertilizer formulations consisting of coconut coir, biochar, lignite, leonardite, microelements, tryptophan, and metabolic products of Pseudomonas sp. and Bacillus subtilis did not play a significant role in improving soil physical characteristics. The lowest productivity was characterized for maize without any fertilizers and amendments. All soil organic amendments resulted in lower yields than the one with only mineral fertilization. The highest root dry matter was obtained when lignite and leonardite were used as main components. Organic amendments can be recommended for soil bioregeneration, but their main effect on maize productivity is attributed to the mineral component.

Plant growth regulators (PGRs) are widely used in turfgrass management. This study aimed to find the influence of different rates of PGRs on Kentucky bluegrass visual quality. Six PGRs were applied, Trinexapac Ethyl, Paclobutrazol, Flurprimidol, Mefluidide, Ethephon, and Gibberellic Acid. The measurement of the leaf color was performed using a spectrophotometer. The turfgrass visual quality was judged using a visual rating system. Trinexapac Ethyl and Flurprimidol applications improved the overall appearance of turfgrass. Paclobutrazol and Gibberellic Acid decreased the overall appearance of investigated Kentucky bluegrass cultivars. The leaf texture assessment was only improved by Gibberellic Acid. The color assessment was enhanced with Paclobutrazol but deteriorated with Gibberellic Acid. PGRs affected the wavelength in a range of 520 to 630 nm. Gibberellic Acid caused lighter leaves with higher green and yellow hues. Paclobutrazol caused darker leaves with a lower green and a reddish hue. Trinexapac Ethyl application resulted in a more reddish hue. PGR rates also affected the visual quality of Kentucky bluegrass turfgrass.