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Rice (Oryza sativa) is a major dietary source of the toxic metal cadmium (Cd). Reducing Cd transfer from soil to the rice grain is important for food safety. Rice cultivars vary widely in their Cd accumulation, but the genetic basis for this variation is not fully understood. Based on field and pot experiments comparing 26 rice cultivars, we identified a cultivar with high Cd accumulation in grain (BG367, coded as W4) and a cultivar with low grain Cd accumulation (Huajingxian 74, coded as W0). W4 showed a higher Cd translocation from roots to shoots than W0. Using chromosome single segment substitution lines derived from the two cultivars, we mapped a quantitative trait locus for Cd accumulation in grain to a 400 kb region in chromosome 7. Using yeast expression assays and transgenic complementation, we identified OsHMA3 as the causal gene at this locus. Compared with OsHMA3W0, OsHMA3W4 has a deletion of 14 amino acids predicted to be in the ATP binding domain. OsHMA3W4 showed a complete loss of transport activity for Cd in yeast assays. Taking our findings together, we have identified a new allele of OsHMA3 with a total loss-of-function, resulting in greatly elevated Cd translocation to rice shoots and grain.

As a medicinal plant, Papaver holds significant value in the medical field. In China, the chemical synthesis of semi-derivatives such as morphine and codeine is limited, with the primary source being Papaver . Understanding the genetic map of Papaver can provide crucial insights into key traits, physiological characteristics, and medicinal properties across different varieties. This information is also valuable for forensic tracing efforts in anti-drug operations. In this study, we performed whole-genome sequencing on 220 samples from 22 species, generating approximately 5.12 TB of paired-end reads with an average coverage depth of 10.2X. After preprocessing, the sequencing data showed a 98.02% alignment rate to the reference genome. A total of 2,790,225 SNPs were identified. The dataset used in this study is the largest publicly available poppy dataset to date. These data offer valuable genetic information for genomic research on local varieties in China and serve as a critical resource for future studies on the pharmacological properties of Papaver , molecular breeding, and genomic tracing.

Papaver somniferum L. is an important medical plant that produces analgesic drugs used for the pain caused by cancers and surgeries. Recent studies have focused on the expression genes involved in analgesic drugs biosynthesis, and the real-time quantitative polymerase chain reaction (RT-qPCR) technique is the main strategy. However, no reference genes have been reported for gene expression normalization in P. somniferum. Herein, nine reference genes (actin (ACT), glyceraldehyde-3-phosphate dehydrogenase (GAPDH), cyclophilin 2 (CYP2), elongation factor 1-alpha (EF-1α), glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic (GAPC2), nuclear cap-binding protein subunit 2 (NCBP2), protein phosphatase 2A (PP2A), TIP41-like protein (TIP41), and tubulin beta chain (TUB)) of P. somniferum were selected and analyzed under five different treatments (cold, drought, salt, heavy metal, and hormone stress). Then, BestKeeper, NormFinder, geNorm, and RefFinder were employed to analyze their gene expression stability. The results reveal that NCBP2 is the most stable reference gene under various experimental conditions. The work described here is the first report regarding on reference gene selection in P. somniferum, which could be used for the accurate normalization of the gene expression involved in analgesic drug biosynthesis.

Opium poppy is one of the most important medicinal plants and remains the only commercial resource of morphinan-based painkillers. However, little is known about the regulatory mechanisms involved in benzylisoquinoline alkaloids (BIAs) biosynthesis in opium poppy. Herein, the full-length transcriptome dataset of opium poppy was constructed for the first time in accompanied with the 33 samples of Illumina transcriptome data from different tissues, growth phases and cultivars. The long-read sequencing produced 902,140 raw reads with 55,114 high-quality transcripts, and short-read sequencing produced 1,923,679,864 clean reads with an average Q30 rate of 93%. The high-quality transcripts were subsequently quantified using the short reads, and the expression of each unigene among different samples was calculated as reads per kilobase per million mapped reads (RPKM). These data provide a foundation for opium poppy transcriptomic analysis, which may aid in capturing splice variants and some non-coding RNAs involved in the regulation of BIAs biosynthesis. It can also be used for genome assembly and annotation which will favor in new transcript identification.Design Type(s)transcription profiling design • sequence annotation objective • organism part comparison designMeasurement Type(s)transcription profiling assayTechnology Type(s)RNA sequencingFactor Type(s)cultivar • developmental stage • organism partSample Characteristic(s)Papaver somniferum • root • stem • leafMachine-accessible metadata file describing the reported data (ISA-Tab format)

s L. is an important medical plant that produces analgesic drugs used for the pain caused by cancers and surgeries. Recent studies have focused on the expression genes involved in analgesic drugs biosynthesis, and the real-time quantitative polymerase chain reaction (RT-qPCR) technique is the main strategy. However, no reference genes have been reported for gene expression normalization in s . Herein, nine reference genes (actin ( ), glyceraldehyde-3-phosphate dehydrogenase ( ), cyclophilin 2 ( ), elongation factor 1-alpha ( - ), glyceraldehyde-3-phosphate dehydrogenase 2, cytosolic ( ), nuclear cap-binding protein subunit 2 ( ), protein phosphatase 2A ( ), TIP41-like protein ( ), and tubulin beta chain ( )) of were selected and analyzed under five different treatments (cold, drought, salt, heavy metal, and hormone stress). Then, BestKeeper, NormFinder, geNorm, and RefFinder were employed to analyze their gene expression stability. The results reveal that is the most stable reference gene under various experimental conditions. The work described here is the first report regarding on reference gene selection in which could be used for the accurate normalization of the gene expression involved in analgesic drug biosynthesis.

There is a common phenomenon in nature whereby some animals have differences in their ontogenetic changes in dietary preferences between sexes, especially apex predators. These reflect changes in the needs of development during their lifetimes. Apex predators potentially have diverse dietary niches and a large impact on the trophic dynamics within ecosystems. However, the difference in life history between males and females often leads to increased difficulty in management and conservation. In this study, 25 oceanic whitetip sharks, Carcharhinus longimanus, were collected from the central and eastern tropical Pacific. Retrospective stable isotope analysis of vertebrae was used to evaluate the potential ontogenetic differences in feeding habits and niche width between sexes. Results showed that C. longimanus had a wide range of δ13C values (−18.1 to −12.3‰) and δ15N values (8.9–14.8‰). However, males and females had similar trophic positions with large niche overlap at similar growth stages. Both sexes had increasing δ13C values but relatively constant δ15N values along the vertebrae. These results indicated that male and female C. longimanus may share similar feeding strategies and movement patterns. The results presented in this study enhance our understanding of sexual ontogenetic patterns and ecological role of C. longimanus and highlighted the applicability of vertebrae for characterizing shark life‐history traits. In this study, 25 oceanic whitetip sharks, Carcharhinus longimanus, were collected from the Central and Eastern Pacific. Retrospective stable isotope analysis of vertebrae was used to evaluate the feeding habits and niche breadth differences between the sexes. The results showed that the oceanic whitetip shark is a long‐lived oceanic pelagic shark with generalist feeding behavior.

Plastic pollution has become a global environmental problem of major concern. However, the plastic contamination in the marine top predators, particularly in endangered species, is incompletely understood because of the limited amount of data on their presence in the digestive system and prey. This study investigated the stomach contents of an endangered but poorly known shark species, the longfin mako shark ( Isurus paucus ), found in the tropical western Pacific Ocean. We examined the plastics in this female specimen (1.22-m fork length) and her prey to assess the potential for trophic transfer of microplastics. Polypropylene bottle cap and lollipop packaging, longnose lancetfish ( Alepisaurus ferox ), and squid were found in the stomach of I. paucus , while no apparent internal injuries were noted. The microplastic fragments and granules, confirmed by laser direct infrared spectroscopy, were found in the digestive system of the intact squid ingested by I. paucus , suggesting that trophic transfer may occur between shark and prey. These results indicate that I. paucus is vulnerable to plastic ingestion and provide evidence of trophic transfer of microplastics in shark species. Our study emphasizes the need to evaluate the potential ecotoxicological consequences of increasing plastic pollution to endangered marine top predators.

In this paper, the recursive aggregation of OWA operators for intuitionistic fuzzy numbers (IFN) based on a non-additive measure (NAM) with σ−λ rules is constructed and investigated in light of the σ−λ rules of a non-additive measure (NAM). Additionally, an integrator is designed by drawing on the genetic algorithm and the process of calculation is elaborated by an example.

In order to identify natural products for plant disease control, the essential oil of star anise (Illicium verum Hook. f.) fruit was investigated for its antifungal activity on plant pathogenic fungi. The fruit essential oil obtained by hydro-distillation was analyzed for its chemical composition by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). trans-Anethole (89.5%), 2-(1-cyclopentenyl)-furan (0.9%) and cis-anethole (0.7%) were found to be the main components among 22 identified compounds, which accounted for 94.6% of the total oil. The antifungal activity of the oil and its main component trans-anethole against plant pathogenic fungi were determined. Both the essential oil and trans-anethole exhibited strong inhibitory effect against all test fungi indicating that most of the observed antifungal properties was due to the presence of trans-anethole in the oil, which could be developed as natural fungicides for plant disease control in fruit and vegetable preservation.