Explore the vast range of titles available.

Introduction. Breast cancer is the world’s most prevalent malignancy, with an increasing incidence and a predisposition for postpubertal females from all cultural and ethnic backgrounds. More recently, oral Fusobacterium nucleatum species have been observed in cancerous human breast tissue, drawing attention to the role of microbes in cancer pathogenesis. Objectives. Investigating oral Fusobacterium nucleatum species as potential biomarkers for female-specific breast cancer. Methods. A systematic search in The Central Register of Controlled Trials, EMBASE, EBSCO, NCBI, and MEDLINE databases was undertaken from the 1st January, 1983–31st March, 2022. Articles included were in English and based on women between the ages of 18–96 years with confirmed gingivitis/periodontal disease and breast cancer diagnoses from registered specialists. Authors extracted data independently, and a meta-analysis of risk estimations measuring associations between oral Fusobacterium nucleatum species and female-specific breast cancer was elucidated via calculated relative risks and 95% confidence intervals. Results. AXIS tool analysis revealed 78.70% of articles with a positive correlation between oral Fusobacterium nucleatum and female-specific breast cancer. The risk of breast cancer development increased with significant levels of oral Fusobacterium nucleatum due to gingivitis/periodontitis (relative risk = 1.78, 95% confidence interval = 1.63–1.91). Low-moderate statistical heterogeneity was found (I2 = 41.39%; P = 0.02), and the importance of periodontal status on breast cancer pathogenesis was determined (relative risk = 1.24, 95% confidence interval = 1.01–1.30). Conclusions. Oral Fusobacterium nucleatum species are a risk factor for breast cancer development, thus elevating their biomarker potentiality.

The popularity of agroecology has grown over the last few years as an alternative paradigm for food systems. This public attention has meant agroecology is increasingly becoming institutionalised and integrated into food policy frameworks. While there is a significant body of literature discussing the origins and worldviews intrinsic to agroecology, hardly any academic publications focusing on analysing policies claiming to have an agroecological focus exist. This first policy study of its kind contributes to the scarce agroecological policy literature by interrogating what we argue is a ‘translation’ process, which starts with the vision of agroecology and analyses how the concept changes once it has been operationalised into a policy document or law. Evidence from two European agricultural policy contexts, namely France and the United Kingdom, is presented. The methodology followed focused on the analysis of the context, problem construction, conceptualisation of agroecology, operational principles, and policy instruments included in the policy documents. Three main themes emerged from the case studies: differences in framing agroecology in the public policy arena; common dependencies to existing configurations influencing translations of agroecology in public policies; and the need for democratic discussion on the hybridisation of agroecology itself, as well as on implied, but often veiled, political choices. This paper concludes that a selective and relational hybridisation of agroecology is emerging during its ‘translation’ into public policies.

Since its inception in the early 80s, inductively coupled plasma–mass spectrometry has developed to the method of choice for the analysis of elements in complex biological systems. High sensitivity paired with isotopic selectivity and a vast dynamic range endorsed ICP-MS for the inquiry of metals in the context of biomedical questions. In a stand-alone configuration, it has optimal qualities for the biomonitoring of major, trace and toxicologically relevant elements and may further be employed for the characterisation of disrupted metabolic pathways in the context of diverse pathologies. The on-line coupling to laser ablation (LA) and chromatography expanded the scope and application range of ICP-MS and set benchmarks for accurate and quantitative speciation analysis and element bioimaging. Furthermore, isotopic analysis provided new avenues to reveal an altered metabolism, for the application of tracers and for calibration approaches. In the last two decades, the scope of ICP-MS was further expanded and inspired by the introduction of new instrumentation and methodologies including novel and improved hardware as well as immunochemical methods. These additions caused a paradigm shift for the biomedical application of ICP-MS and its impact in the medical sciences and enabled the analysis of individual cells, their microenvironment, nanomaterials considered for medical applications, analysis of biomolecules and the design of novel bioassays. These new facets are gradually recognised in the medical communities and several clinical trials are underway. Altogether, ICP-MS emerged as an extremely versatile technique with a vast potential to provide novel insights and complementary perspectives and to push the limits in the medical disciplines. This review will introduce the different facets of ICP-MS and will be divided into two parts. The first part will cover instrumental basics, technological advances, and fundamental considerations as well as traditional and current applications of ICP-MS and its hyphenated techniques in the context of biomonitoring, bioimaging and elemental speciation. The second part will build on this fundament and describe more recent directions with an emphasis on nanomedicine, immunochemistry, mass cytometry and novel bioassays.

There has been a recent surge of interest in the use of superparamagnetic iron oxide nanoparticles (SPIONs) as contrast agents (CAs) for magnetic resonance imaging (MRI), due to their tunable properties and their low toxicity compared with other CAs such as gadolinium. SPIONs exert a strong influence on spin-spin T2 relaxation times by decreasing the MR signal in the regions to which they are delivered, consequently yielding darker images or negative contrast. Given the potential of these nanoparticles to enhance detection of alterations in soft tissues, we studied the MRI response of hydrophobic or hydrophilic SPIONs loaded into liposomes (magnetoliposomes) of different lipid composition obtained by sonication. These hybrid nanostructures were characterized by measuring several parameters such as size and polydispersity, and number of SPIONs encapsulated or embedded into the lipid systems. We then studied the influence of acyl chain length as well as its unsaturation, charge, and presence of cholesterol in the lipid bilayer at high field strength (7 T) to mimic the conditions used in preclinical assays. Our results showed a high variability depending on the nature of the magnetic particles. Focusing on the hydrophobic SPIONs, the cholesterol-containing samples showed a slight reduction in r2, while unsaturation of the lipid acyl chain and inclusion of a negatively charged lipid into the bilayer appeared to yield a marked increase in negative contrast, thus rendering these magnetoliposomes suitable candidates as CAs, especially as a liver CA.

Of note, HIV-exposed children have increased morbidity and mortality in early childhood compared with children born to uninfected women [5]. [...]both HIV exposure and HIV infection have been associated with increased prevalence of severe malarial anemia during acute Plasmodium falciparum infection [6]. Sierra Leone was the first country to implement PMC as malaria control strategy nationwide in 2018. Since 2021, an increasing number of countries in sub-Saharan Africa have adopted the strategy and are starting its implementation [9,10]. A public health paradox and a “déjà vu” Despite HIV-exposed children having a higher risk of severe malaria than HIV-unexposed children, they are not eligible to receive PMC with SP [5]. [...]paradoxically, the most vulnerable children, those who are under 2 years of age and HIV-exposed, are currently the least protected against malaria by chemoprevention strategies. [...]decades of experience in the evaluation of malaria prevention with intermittent preventive treatment in pregnancy for women living with HIV on CTX prophylaxis should be a lesson to not leave their

Abstract Inductively coupled–plasma mass spectrometry (ICP-MS) has transformed our knowledge on the role of trace and major elements in biology and has emerged as the most versatile technique in elemental mass spectrometry. The scope of ICP-MS has dramatically changed since its inception, and nowadays, it is a mature platform technology that is compatible with chromatographic and laser ablation (LA) systems. Over the last decades, it kept pace with various technological advances and was inspired by interdisciplinary approaches which endorsed new areas of applications. While the first part of this review was dedicated to fundamentals in ICP-MS, its hyphenated techniques and the application in biomonitoring, isotope ratio analysis, elemental speciation analysis, and elemental bioimaging, this second part will introduce relatively current directions in ICP-MS and their potential to provide novel perspectives in the medical sciences. In this context, current directions for the characterisation of novel nanomaterials which are considered for biomedical applications like drug delivery and imaging platforms will be discussed while considering different facets of ICP-MS including single event analysis and dedicated hyphenated techniques. Subsequently, immunochemistry techniques will be reviewed in their capability to expand the scope of ICP-MS enabling analysis of a large range of biomolecules alongside elements. These methods inspired mass cytometry and imaging mass cytometry and have the potential to transform diagnostics and treatment by offering new paradigms for personalised medicine. Finally, the interlacing of immunochemistry methods, single event analysis, and functional nanomaterials has opened new horizons to design novel bioassays which promise potential as assets for clinical applications and larger screening programs and will be discussed in their capabilities to detect low-level proteins and nucleic acids.

Agriculture, together with aquaculture, supplies most of the foodstuffs required by the world human population to survive. Hence, bacterial diseases affecting either agricultural crops, fish, or shellfish not only cause large economic losses to producers but can even create food shortages, resulting in malnutrition, or even famine, in vulnerable populations. Years of antibiotic use in the prevention and the treatment of these infections have greatly contributed to the emergence and the proliferation of multidrug-resistant bacteria. This review addresses the urgent need for alternative strategies for the use of antibiotics, focusing on the use of bacteriophages (phages) as biocontrol agents. Phages are viruses that specifically infect bacteria; they are highly host-specific and represent an environmentally-friendly alternative to antibiotics to control and kill pathogenic bacteria. The information evaluated here highlights the effectiveness of phages in the control of numerous major pathogens that affect both agriculture and aquaculture, with special emphasis on scientific and technological aspects still requiring further development to establish phagotherapy as a real universal alternative to antibiotic treatment.

Background With a human immunodeficiency virus (HIV) prevalence of 2.1% among 15–24 year olds, opportunities for further integration of sexual and reproductive health (SRH) and HIV prevention services for young people in Uganda exist. We examine a range of factors that contribute to variance in risky sexual behaviour among young Ugandans. Methods A cross-sectional, nationally representative household survey was conducted between February and March 2016. The questionnaire used assessed knowledge, attitudes and practices related to SRH among young people aged 10–24 years. A composite indicator was constructed to assess risky sexual behaviour, defined as being involved in sexual relations under the influence of alcohol or drugs, engaging in sexual activities without a condom, and having multiple sexual partners in the 6 months preceding the survey. Exploratory analysis was conducted to provide descriptive statistics. Logistic regression was conducted to determine the factors associated with risky sexual behaviour. This analysis focuses on the sub-category aged 15–24 years, comprised of 2725 respondents. Results Knowledge levels of family planning (FP), sexually transmitted infections (STIs) and HIV across all respondents were high (above 82%). Self-reported perceived risk of STIs and pregnancy was consistently higher among 20–24 year old respondents, with 61.5% feeling at risk of STIs compared to 46.2% of 15–19 year olds. A total of 22.7% of respondents reported having been involved in risky sexual behaviour. Factors associated with risky sexual behaviour among the 15–19 years group included gender, single orphanhood, casual work, schooling status, FP knowledge and self-perceived risk of STIs/HIV. For the 20–24 year old respondents, significantly associated factors included gender, educational level, relationship to head of household, place of residence, and self-perceived risk of pregnancy. Conclusions Despite high general SRH/HIV knowledge and perceived risk of pregnancy and HIV, risky sexual behaviour among young Ugandans remains high. Effectiveness gaps in the integrated SRH/HIV response for young people should be addressed and targeted interventions focused on holistic prevention at individual level through information, risk awareness, and skill development should be combined with interventions targeting social structures affecting individual behaviour.

Matrix metalloproteinases (MMPs) are a family of zinc-dependent endopeptidases. The main functions of these metalloproteinases are the degradation of the stromal connective tissue and basement membrane components. Recent data from model systems suggest that MMPs are involved in breast cancer (BC) initiation, invasion, and metastasis. Particularly, MMP-11 (stromelysin-3) is expressed in stromal fibroblasts adjacent to epithelial tumor cells, and high levels of this metalloproteinase were associated with tumor progression and poor prognosis of BC. Consequently, MMP-11 involved in these processes can be a candidate as a new potential prognostic biomarker in BC. Bioimaging techniques based on laser ablation/desorption and mass spectrometry are rapidly growing in biology and medicine for studies of biological systems to provide information of biomolecules (such as proteins, metabolites, and lipids) and metals with lateral resolution at the micrometer scale. In this study, matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI-MSI) has been used for the first time to investigate the distribution of MMP-11 in human breast cancer tissues in order to show a possible correlation between cancerous and healthy samples, by differential proteomics and using such differences for possible cancer diagnosis and/or prognosis. Additionally, those human breast tissue samples were analyzed in parallel by laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) in order to gather additional information about the elemental distribution of Zn and its possible associations with MMPs.

Understanding the microscopic variability of impurities in glacier ice on a quantitative level has importance for assessing the preservation of paleoclimatic signals and enables the study of macroscopic deformational as well as dielectric ice properties. Two‐dimensional imaging via laser‐ablation‐inductively‐coupled‐plasma‐mass‐spectrometry (LA‐ICP‐MS) can provide key insight into the localization of impurities in the ice. So far, these findings are mostly qualitative and gaining quantitative insights remains challenging. Recent advances in LA‐ICP‐MS high‐resolution imaging now allow ice grains and grain boundaries to be resolved individually. These resolutions require new adequate quantification strategies and, consequently, accurate calibration with matrix‐matched standards. Here, we present three different quantification methods, which provide a high level of homogeneity at the scale of a few tens of microns and are dedicated to imaging applications of ice cores. One of the proposed methods has a second application, offering laboratory experiments to investigate the displacement of impurities by grain growth, with important future potential to study ice‐impurity interactions. Standards were analyzed to enable absolute quantification of impurities in selected ice core samples. Calibrated LA‐ICP‐MS maps indicate similar spatial distributions of impurities in all samples, while impurity levels vary distinctly: Higher concentrations were detected in glacial periods and Greenland, and lower levels in interglacial periods and samples from central Antarctica. These results are consistent with ranges from complementary meltwater analysis. Further comparison with cm‐scale melting techniques calls for a more sophisticated understanding of the ice chemistry across spatial scales, to which calibrated LA‐ICP‐MS maps now contribute quantitatively. Plain Language Summary Compared to the large amount of information relating to paleoclimate signals reconstructed from cm‐scale impurity measurements on ice cores, knowledge about the spatial variability of impurities at the micro‐scale is extremely sparse—and becomes even more rare once quantitative datasets are concerned. However, there is an increasing demand for quantitative data for assessing the preservation of paleoclimatic signals and for the study of macroscopic deformational as well as dielectric ice properties in ice flow modeling and remote sensing. Two‐dimensional imaging via laser‐ablation‐inductively‐coupled‐plasma‐mass‐spectrometry (LA‐ICP‐MS) has shown great potential in this context, but so far, gaining reliable quantitative results for micro‐scale imaging has not been possible. Here, we present new quantification strategies that finally allow accurate calibration using ice standards. We carefully discuss the pros and cons of each method, apply the calibration to different samples from Greenland and Antarctica, and deliver the first calibrated LA‐ICP‐MS impurity maps at 40 μm resolution. Our results are consistent with bulk measurements performed on melted samples. The calibrated LA‐ICP‐MS maps will be essential for further comparison with bulk meltwater analysis, which may ultimately deliver an improved understanding of paleoclimate signals stored in deep ice. Key Points This study presents new quantification strategies for two‐dimensional micro‐scale impurity imaging on ice cores with laser‐ablation‐inductively‐coupled‐plasma‐mass‐spectrometry (LA‐ICP‐MS) Calibrated LA‐ICP‐MS maps reveal similar spatial distributions of impurities in all ice core samples, while concentrations vary distinctly We developed a method to investigate the displacement of impurities by grain growth and to study ice‐impurity interactions in the laboratory