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To evaluate the impact of nutritional tea polyphenols (TPs) on body composition, growth, biochemical markers, antioxidant capacity, and hemato-immunological levels, a ten-week feeding experiment was carried out on coho salmon (Oncorhynchus kisutch, 180.51 ± 0.15 g). The control group was fed a basal diet; the T1, T2, T3, and T4 groups were fed 0.005%, 0.01%, 0.02%, and 0.04% TPs, respectively. These results demonstrate that adding TPs significantly (p < 0.05) increased the coho salmon fish’s weight gain (WG), relative growth rate (RGR), condition factor (CF), feed efficacy (FE), daily growth rate (DGR), and specific growth rate (SGR). There was no discernible difference in the body compositions of the treated TPs and the control group (p > 0.05). In addition, the T3 group showed a significant (p < 0.05) decrease in GPT, LDL, HDL, TC, and CAT. Fish given a 0.02% diet containing TPs had significantly lower levels of malondialdehyde (MDA) in their liver; yet, the TP-treated groups had higher levels of SOD and CAT than the control (p < 0.05). The data analysis shows a significant rise in lysozyme, respiratory burst activity, bactericidal activity, and blood hematological parameters in the 0.01–0.04% TP groups. According to these findings, TPs could be a useful dietary supplement for raising the antioxidant status, growth parameters, haemato-immunological response, and whole-body composition of coho salmon fish.

Vitamin K3 (VK3) is an essential micronutrient for fish growth and metabolism. To determine the optimal required dietary concentration of VK, a 12-week-long trial was planned to investigate the impact of VK3 on growth performance, muscle composition, liver menaquinone-4 (MK-4), whole-body MK-4 concentration, and antioxidant capacity (T-AOC) in coho salmon alevins. A total of 2100 coho salmon alevins were divided into twenty-one tanks, with 100 alevins in each tank, and three tanks represented one group. Each of the seven groups were randomly assigned to one of the experimental diets formulated with varying levels of menadione nicotinamide bisulphite (VK3), i.e., 0.16 (control), 5.25, 10.22, 14.93, 20.51, 40.09, and 59.87 mg/kg, respectively. The results revealed that the inclusion of VK3 from 5.25 to 40.09 mg/kg improved (p < 0.05) the survival rate (SR), weight gain (WG), specific growth rate (SGR), and feed conversion ratio (FCR), while no effect (p > 0.05) was observed on the hepatosomatic index (HSI), viscerosomatic index (VSI), Fulton’s condition factor (K), and muscle composition. The whole-body MK-4, liver MK-4, liver T-AOC, total superoxide dismutase (T-SOD), and catalase (CAT) enzymes were also increased (p < 0.05). Malondialdehyde (MDA) contents were reduced (p < 0.05) in all supplemented groups with a decreasing trend. The predicted optimal required dietary concentrations of VK3 were found to be 34.0, 43.5, 38.54, and 31.97 mg/kg based on WG, SGR, liver MK-4 concentration, and T-AOC, respectively, by quadratic regression analysis. It is concluded that the dietary VK3 has improved the growth performance and antioxidant status in alevins during early development in the range of 31.97 to 43.5 mg/kg diet.

In this study, the effects of dietary supplementation of Indian Sphagnum junghuhnianum peat moss pectin were evaluated in Catla catla fingerlings. A total of 375 fish (10.47 ± 0.2 g) were randomly divided ( n  = 25/tank) into basal diet (Sphagnum peat moss pectin [SPMP] at 0% control) (crude protein: 37%, crude fat: 8%) or four treatment diets, 2% (SPMP2), 4% (SPMP4), 8% (SPMP8), and 16% (SPMP16) for 60 days. The water temperature was 27.5 ± 0.6°C, and fish were fed with the experimental diets at 4% of their live body mass. The results of this research revealed that the SPMP8 diet substantially enhanced growth indices in catla fingerlings ( p < 0.05). The highest and lowest whole‐body lipid levels were in SPMP8 (5.03%) and control (4.06%), respectively. The gut amylase activity was significantly higher in the SPMP8 group, while lipase and protease remained unchanged. Additionally, fish given 8% and 16% SPMP had a significant decrease in the liver’s malondialdehyde concentration. Superoxide dismutase (SOD) activity was increased in SPMP‐supplemented groups, with the highest value in group SPMP8 while catalase (CAT) activity was higher in SPMP4, SPMP8, and SPMP16 compared to the other groups. Supplementing the diet with 4%–16% SPMP increased the fish’s red blood cell count and hemoglobin level. An 8% SPMP diet increased white blood cell count, phagocytic activity, lysozyme, acid, and alkaline phosphatase (AKP) activities compared to the other groups. The cumulative mortality of fish after challenge with virulent Aeromonas hydrophila significantly decreased in SPMP8 and SPMP16 groups, compared to the other treatments. The results of this study indicated enhancements in immunological markers, specifically an increase in levels of AKP, SOD, and CAT. This research proposes that providing catla fingerlings with 8% SPMP effectively boosts their growth, immune response, and disease resistance.

Vitamin A (VA) is an essential micronutrient that improves growth, immune activity, and antioxidant responses in fish. This study focuses on optimizing VA dietary levels for Oncorhynchus kisutch (Coho salmon) alevins. A 12-week trial was conducted using seven diets containing graded dietary VA levels of 244, 957, 1902, 3494, 6906, 10,248, and 25,213 IU/kg. A total of 2100 fish were reared in 21 tanks; 100 fish were housed in each tank, and 3 tanks represented one treatment. Peak (SGR) and FBW were observed at 6906 IU/kg. Excess VA levels (>15,000 IU/kg) compromised feed conversion efficacy and led to oxidative stress. Analysis of proximate composition resulted in protein and lipid deposition at optimal VA levels. However, excess may have led to metabolic disturbances and reduced ash content. The activity of the antioxidant enzymes catalase (CA), acid phosphatase (ACP), alkaline phosphatase (AKP), and total superoxide dismutase (T-SOD) revealed biphasic patterns, peaking at 6906 IU/kg and dropping when VA levels were exceeded, inducing pro-oxidant effects. Malondialdehyde (MDA), the indicator of toxicity, had a minimal value of 15,000 IU/kg. VA accumulation in the liver showed a dose-dependent relationship, while excess storage (>25,000 IU/kg) induced hepatotoxicity. Quadratic regression was used to identify the optimum VA levels required in Coho salmon alevins, ranging from 6906 to 10,248 IU/kg. Polynomial quadratic regression results indicated that the predicted dietary inclusion of VA at 3000 IU/kg and 15,000 IU/kg may yield better results of T-SOD and MDA. Real-world experimentation is recommended to explore long-term VA optimization with other nutrients and promote better feed utilization and sustainable aquaculture practices.

Macroalgae plays a significant role in the formulation of innovative and environmentally sustainable approaches to address food challenges. Specifically, green macroalgae serve as dietary supplements aimed at improving the health, growth, and feeding efficiency of various species of marine and freshwater fishes, as well as mollusks. The effects of Chaetomorpha linum extract (CLE) on growth performance, physiological responses, and disease resistance are studied in Bellamya bengalensis against Aeromonas hydrophila. In this experiment, adult B. bengalensis (4412 ± 165.25 mg) were randomly divided into 15 rectangular glass aquariums (35 snail/aquaria; 45 L capacity) and their basal diet was supplemented with different levels of CLE, including 0 (CLE0), 1 (CLE1), 2 (CLE2), 3 (CLE3), and 4 (CLE4) g/kg for 60 days. The growth performance in the CLE3 dietary group was significantly higher that of the CLE0 group, exhibiting both linear and quadratic trends in relation to dietary CLE levels (p < 0.05). The activities of pepsin, amylase, and lipase were found to be highest in CLE3 and lowest in CLE0. Both linear and quadratic responses to dietary CLE levels in digestive enzymes were observed (p < 0.05). The activities of superoxide dismutase and catalase in the hepatopancreas were found to be elevated in snails due to the synergistic effect of the supplemented CLE diet. Among different levels of diet given, CLE2-supplemented snails showed an increase in their enzyme activity (p < 0.05). Interestingly, all the CLE-treated snails expressed elevated levels of mucus lysozyme and mucus protein when compared to control (p < 0.05). Additionally, hepatopancreatic acid phosphatase and alkaline phosphatase activity were elevated in snails consuming CLE3 (p < 0.05). The transcription levels of immune-related genes, including mucin-5ac and cytochrome, were significantly elevated in snails that were fed a diet supplemented with 2–4 g of CLE/kg. Furthermore, the transcription level of the acid phosphatase-like 7 protein gene also increased in snails receiving CLE-supplemented diets. After a 14-day period of infection, snails that consumed a diet supplemented with 3–4 g/kg of CLE exhibited a notable increase in survival rates against virulent A. hydrophila. Based on the above findings, it is suggested that a diet supplemented with 3 g/kg of CLE may enhance growth, antioxidant and immune defense, and disease resistance in the freshwater snail B. bengalensis.

Due to the increasing trend in aquaculture and fisheries production, conventional feed resources, especially protein ingredients, are overburdened, and sometimes their price fluctuations limit quality feed formulations for farmers, thus leading to the exploration of alternative protein feed resources. Antarctic krill meal (AKM) has emerged as a potential candidate ingredient for sustainable and suitable partial replacement of conventional fish meal. A 10-week feeding trial was designed to investigate the effects of different concentrations of AKM on the growth performance, antioxidant capacity, and serum biochemical indices of coho salmon ( Oncorhynchus kisutch ) post-smolts. In total, 150 post-smolt fish with an average initial weight of 178.17 ± 0.73 g were divided into five groups with three replicates in each group and 10 fish in each replicate. Five experimental diets were formulated by substituting 0%, 3%, 6%, 9%, and 12% fish meal (protein source) with AKM (protein source), which were designated AKM0 (the control), AKM3, AKM6, AKM9, and AKM12, respectively. The results showed that the addition of AKM to the salmon diet increased (P < 0.05) the weight gain (WG), specific growth rate (SGR), feed conversion ratio (FCR), and protein efficiency ratio (PER). There was no effect (P > 0.05) on body composition and essential amino acid profile of the meat except for methionine, isoleucine, lysine, and threonine. The liver concentrations of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and malondialdehyde (MDA) significantly decreased while the concentration of catalase and superoxide dismutase (SOD) increased in the AKM supplemented groups compared to the control group. The serum concentrations of ALT and AST decreased (P < 0.05) while total cholesterol, triglyceride, and total protein content increased (P < 0.05) in the AKM treatment groups compared to the control. Based on the results of this study, supported by polynomial quadratic regression analyses of WG, SGR, FCR, and PER, we conclude that 6.73%–7.08% AKM is the best possible suitable inclusion level range to partially replace conventional fish meal in the diet of coho salmon post-smolt.

The current study focused on assessing the outcome of methanol extract of Chaetomorpha antennina (MECA) on the growth performance and immune modulation in both specific and non-specific immune responses through the assessment of neutrophil, serum lysozyme, serum myeloperoxidase, antiprotease, ceruloplasmin, reactive oxygen species (ROS), and reactive nitrogen species (RNS) activity in Labeo rohita (rohu) at 28 days post treatment along with assessment of the disease resistance capacity against Edwardsiella tarda at 30days post immunization. Fishes (n = 144; average weight 50.0 ± 0.23 g) were evenly divided into four treatments, with 12 fishes per tank in triplicates. The MECA was injected intraperitoneally in the fishes at different doses as 0, 25, 75, and 150 mg/kg of the body weight. The results demonstrated that fish treated with MECA have an increased body weight, specific growth rate, and feed conversion ratio (p < 0.05) with respect to the control group. Results suggested that the MECA inclusion can significantly enhance (p < 0.05) the levels of serum lysozyme, neutrophil function, serum antiprotease activity, cellular RNS, and ROS production. Exposure to MECA of 75 mg/kg showed a significantly higher survival percentage against E. tarda disease infection. These results indicate MECA as a stimulant of immunity in L. rohita against E. tarda. The results suggested that MECA is a potent immunostimulant in finfish aquaculture and can offer higher economic welfare.

The research aimed to analyze the influences of adding marine green algae Chaetomorpha aerea to the diet of guppy fish (Poecillia reticulate) on growth, immunological responses in skin mucus, total carotenoid content, and sexual characteristics. A total of 450 fish, with a mean body weight of 0.19 ± 0.1 g and 30 fish per tank (triplicate), were randomly fed into 15 experimental tanks, each containing 50 L. Five different diets with 0, 1, 2, 4, 8, and 10% of C. aerea g/kg diets were fed to P. reticulate for 30 days. After 30 days, growth, immunological responses in skin mucus, total carotenoid content, and sexual characteristics were investigated. The results observed that the feed conversion rate and fry output were significantly (p > 0.05) decreased in experimental groups compared to the control group. The results revealed that the dietary inclusion of C. aerea algal significantly increased (p < 0.05) in mucosal immunological parameters containing lysozyme activity, myeloperoxidase activity, total immunoglobulins, and alternative complement activity, which were the highest in the group with 4% of C. aerea g/kg. Additionally, lateral skin and the caudal fin of fish had higher total carotenoid levels from the dietary C. aerea algae diet than the control group, which were the highest in the groups with 4%. Among them, 4 and 8% of C. aerea g/kg diet resulted in better growth performance and feed conversion ratio. Thus, the study suggested that 4% of C. aerea g/kg diet has enrichment of immunity, total carotenoid concentrations, and skin mucus immunity of P. reticulate.

In this study, the effects of a mixed algal blend (Chlorella vulgaris, Euglena viridis, and Spirulina platensis) at different levels were evaluated on growth, hematological immune responses, and expression of immune genes in Labeo rohita against post-challenges of Aeromonas hydrophila. Fish samples were fed a diet containing different levels of mixed blend algal (0, 0.01, 0.02, 0.04 and 0.08% of basal diet). At the end of the feeding period, the fish were challenged with A. hydrophila and fish mortality was recorded over a 14-days period. To evaluate the serum biochemical (albumin, globulin), hematological parameters (Hb, RBC and WBC) and immune parameters (neutrophil activity, lysozyme activity, myeloperoxidase activity, antiprotease activity, ceruloplasmin activity, and bactericidal activity), as well as the expression of immune genes (NKEF-B, Lysozyme C and G, TNF α, TLR22, β2M, and β-actin), fish were sampled on Day 7, 14, 21 and 28. Fish were challenged with virulent A. hydrophila 30 days post-feeding and mortalities were recorded over 30 days post-infection. Results demonstrate that fish fed with a mixed algal blend showed that total body weight gain, specific growth rate, total serum protein, globulin, total hemoglobin content, white blood cells, neutrophil, lysozyme, bactericidal, myeloperoxidase, and antiprotease activity in dietary algae blended application was higher than in the control (p < 0.05). According to the results, relative expression of target genes showed significant increases of 0.02 to 0.04% in the treatment group compared to the control group (p < 0.05). At the end of the 30-day exposure to A. hydrophila, the fish that received the mixed algal blend had a significantly higher rate of survival than the control group, with the highest survival rate recorded in the 0.02% mixed algal blend (p < 0.05). According to the effective results of the mixed algal blend on stimulating the immune system and increasing fish resistance to A. hydrophila, it is recommended to use 0.02 to 0.04% of this mixed algal blend in rohu, L. rohita diets.

The increased prevalence of disease, mortality, and antibiotic resistance among aquatic microorganisms has renewed interest in non-conventional disease prevention and control approaches. Nanoparticles present several benefits in aquaculture and hold significant potential for controlling both human and animal infections. This study reports on the antibacterial properties of green copper oxide nanoparticles (CuO NPs) synthesized from the urine of Mithun (MU) (Bos frontalis). In addition, an array of analytical techniques, including scanning electron microscopy (SEM), X-ray diffraction (XRD), UV-visible spectroscopy (UV), and Fourier transform infrared spectroscopy (FTIR), were employed to investigate the synthesized MU-CuO nanoparticles. Aeromonas hydrophila and Aeromonas veronii, two bacterial fish pathogens known to cause severe infectious diseases in fish, were tested for their antibacterial efficacy against MU-CuO NPs. At 100 µg/mL, MU-CuO NPs exhibit enhanced antibacterial efficacy against two bacterial pathogens commonly found in fish. Applications in aquaculture may be looked at given that MU-CuO NPs showed greater antibacterial activity.