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"Grimley, Chris"
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Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study
12 microRNA expression profiling platforms are compared for their reproducibility, sensitivity, accuracy and specificity, and the strengths and weaknesses of each platform are discussed.
MicroRNAs are important negative regulators of protein-coding gene expression and have been studied intensively over the past years. Several measurement platforms have been developed to determine relative miRNA abundance in biological samples using different technologies such as small RNA sequencing, reverse transcription–quantitative PCR (RT-qPCR) and (microarray) hybridization. In this study, we systematically compared 12 commercially available platforms for analysis of microRNA expression. We measured an identical set of 20 standardized positive and negative control samples, including human universal reference RNA, human brain RNA and titrations thereof, human serum samples and synthetic spikes from microRNA family members with varying homology. We developed robust quality metrics to objectively assess platform performance in terms of reproducibility, sensitivity, accuracy, specificity and concordance of differential expression. The results indicate that each method has its strengths and weaknesses, which help to guide informed selection of a quantitative microRNA gene expression platform for particular study goals.
Journal Article
Imagining the modern : architecture and urbanism of the Pittsburgh Renaissance
\"In the 1950s and '60s an ambitious program of urban revitalization transformed Pittsburgh and became a model for other American cities. Billed as the Pittsburgh Renaissance, this era of superlatives-the city claimed the tallest aluminum clad building, the world's largest retractable dome, the tallest steel structure-developed through visionary mayors and business leaders, powerful urban planning authorities, and architects and urban designers of international renown, including Frank Lloyd Wright, I.M. Pei, Mies van der Rohe, SOM, and Harrison & Abramovitz. These leaders, civic groups, and architects worked together to reconceive the city through local and federal initiatives that aimed to address the problems that confronted Pittsburgh's postwar development. Initiated as an award-winning exhibition at the Carnegie Museum of Art in 2014, Imagining the Modern untangles this complicated relationship with modern architecture and planning through a history of Pittsburgh's major sites, protagonists, and voices of intervention. Through original documentation, photographs and drawings, as well as essays, analytical drawings, and interviews with participants, this book provides a nuanced view of this crucial moment in Pittsburgh's evolution. Addressing both positive and negative impacts of the era, Imagining the Modern examines what took place during the city's urban renewal era, what was gained and lost, and what these histories might suggest for the city's future\"-- Provided by publisher.
Book
The Interior Design Reference & Specification Book
In the world of interior design, thousands of bits of crucial information are scattered across a wide array of sources. The Interior Design Reference & Specification Book collects the information essential to planning and executing interior projects of all shapes and sizes, and distills it in a format that is as easy to use as it is to carry.
eBook
Correction: Corrigendum: Evaluation of quantitative miRNA expression platforms in the microRNA quality control (miRQC) study
Nat. Methods 11, 809–815 (2014); published online 29 June 2014; corrected after print 30 July 2014 In the version of this article initially published, the author Linda Wong was omitted from the author list. The error has been corrected in the HTML and PDF versions of the article.
Journal Article
