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The conversion of light into usable chemical and mechanical energy is pivotal to several biological and chemical processes, many of which occur in solution. To understand the structure–function relationships mediating these processes, a technique with high spatial and temporal resolutions is required. Here, we report on the design and commissioning of a liquid-phase mega-electron-volt (MeV) ultrafast electron diffraction instrument for the study of structural dynamics in solution. Limitations posed by the shallow penetration depth of electrons and the resulting information loss due to multiple scattering and the technical challenge of delivering liquids to vacuum were overcome through the use of MeV electrons and a gas-accelerated thin liquid sheet jet. To demonstrate the capabilities of this instrument, the structure of water and its network were resolved up to the 3 rd hydration shell with a spatial resolution of 0.6 Å; preliminary time-resolved experiments demonstrated a temporal resolution of 200 fs.

Newly developed millennial δ 13 C larch tree-ring chronology from Siberia allows reconstruction of summer (July) vapor pressure deficit (VPD) changes in a temperature-limited environment. VPD increased recently, but does not yet exceed the maximum values reconstructed during the Medieval Warm Anomaly. The most humid conditions in the Siberian North were recorded in the Early Medieval Period and during the Little Ice Age. Increasing VPD under elevated air temperature affects the hydrology of these sensitive ecosystems by greater evapotranspiration rates. Further VPD increases will significantly affect Siberian forests most likely leading to drought and forest mortality even under additional access of thawed permafrost water. Adaptation strategies are needed for Siberian forest ecosystems to protect them in a warming world.

We are building a transition edge sensor (TES) X-ray spectrometer for the Linac Coherent Light Source (LCLS-II) at SLAC National Accelerator Laboratory (SLAC) to coincide with new upgrades for this free electron laser facility. This new X-ray spectrometer will have 1000 TES pixels with 0.5 eV energy resolution for soft X-rays below 1 keV. Multiplexing will be done with microwave SQUID resonators and new specialized electronic hardware developed at SLAC. This spectrometer will use a dilution refrigerator to achieve lower operating temperatures than previous TES spectrometers and will be coupled to the liquid jet endstation at LCLS-II. The spectrometer is designed to operate at much higher count rates than previous TES X-ray spectrometers to take advantage of the high repetition rate of the LCLS-II. Science applications will utilize the high photon collection efficiency and throughput, high energy resolution, as well as its ability to simultaneously measure its full calibrated energy range.

Polar ice core records attest to a colossal volcanic eruption that took place ca. A.D. 1257 or 1258, most probably in the tropics. Estimates based on sulfate deposition in these records suggest that it yielded the largest volcanic sulfur release to the stratosphere of the past 7,000 y. Tree rings, medieval chronicles, and computational models corroborate the expected worldwide atmospheric and climatic effects of this eruption. However, until now there has been no convincing candidate for the mid-13th century “mystery eruption.” Drawing upon compelling evidence from stratigraphic and geomorphic data, physical volcanology, radiocarbon dating, tephra geochemistry, and chronicles, we argue the source of this long-sought eruption is the Samalas volcano, adjacent to Mount Rinjani on Lombok Island, Indonesia. At least 40 km ³ (dense-rock equivalent) of tephra were deposited and the eruption column reached an altitude of up to 43 km. Three principal pumice fallout deposits mantle the region and thick pyroclastic flow deposits are found at the coast, 25 km from source. With an estimated magnitude of 7, this event ranks among the largest Holocene explosive eruptions. Radiocarbon dates on charcoal are consistent with a mid-13th century eruption. In addition, glass geochemistry of the associated pumice deposits matches that of shards found in both Arctic and Antarctic ice cores, providing compelling evidence to link the prominent A.D. 1258/1259 ice core sulfate spike to Samalas. We further constrain the timing of the mystery eruption based on tephra dispersal and historical records, suggesting it occurred between May and October A.D. 1257.

The mid-17th century is characterized by a cluster of explosive volcanic eruptions in the 1630s and 1640s, climatic conditions culminating in the Maunder Minimum, and political instability and famine in regions of western and northern Europe as well as China and Japan. This contribution investigates the sources of the eruptions of the 1630s and 1640s and their possible impact on contemporary climate using ice core, tree-ring, and historical evidence but will also look into the socio-political context in which they occurred and the human responses they may have triggered. Three distinct sulfur peaks are found in the Greenland ice core record in 1637, 1641–1642, and 1646. In Antarctica, only one unambiguous sulfate spike is recorded, peaking in 1642. The resulting bipolar sulfur peak in 1641–1642 can likely be ascribed to the eruption of Mount Parker (6∘ N, Philippines) on 26 December 1640, but sulfate emitted from Komaga-take (42∘ N, Japan) volcano on 31 July 1641 has potentially also contributed to the sulfate concentrations observed in Greenland at this time. The smaller peaks in 1637 and 1646 can be potentially attributed to the eruptions of Hekla (63∘ N, Iceland) and Shiveluch (56∘ N, Russia), respectively. To date, however, none of the candidate volcanoes for the mid-17th century sulfate peaks have been confirmed with tephra preserved in ice cores. Tree-ring and written sources point to cold conditions in the late 1630s and early 1640s in various parts of Europe and to poor harvests. Yet the early 17th century was also characterized by widespread warfare across Europe – and in particular the Thirty Years' War (1618–1648) – rendering any attribution of socio-economic crisis to volcanism challenging. In China and Japan, historical sources point to extreme droughts and famines starting in 1638 (China) and 1640 (Japan), thereby preceding the eruptions of Komaga-take (31 July 1640) and Mount Parker (4 January 1641). The case of the eruption cluster between 1637 and 1646 and the climatic and societal conditions recorded in its aftermath thus offer a textbook example of difficulties in (i) unambiguously distinguishing volcanically induced cooling, wetting, or drying from natural climate variability and (ii) attributing political instability, harvest failure, and famines solely to volcanic climatic impacts. This example shows that while the impacts of past volcanism must always be studied within the contemporary socio-economic contexts, it is also time to move past reductive framings and sometimes reactionary oppositional stances in which climate (and environment more broadly) either is or is not deemed an important contributor to major historical events.

The taxon Forficula auricularia L. (Dermaptera: Forficulidae) is a complex of two sibling species that differ in life history (number of clutches per year and imaginal diapause) and that have diverged at the molecular level. The study of a contact zone in the Pyrenean Mountains, using the PCR-RFLP method on two mitochondrial regions (the 16S rRNA and the Cytochrome Oxidase intergenic region), revealed the coexistence of the sibling species at intermediate altitude (1200 m) whereas at lower and higher altitudes only one species was found. An allozyme study, conducted simultaneously and based on four polymorphic loci ( PGI1 , AAT1 , Est-P1 and Est-P2 ), showed no sign of nuclear introgression. The apparent lack of hybridization in the field is consistent with a postzygotic barrier observed in the laboratory (a nearly complete failure to produce F 1 hybrids). This contact zone is probably a sympatric zone between two genetically differentiated species.

Most mouse models of hepatocellular carcinoma have expressed growth factors and oncogenes under the control of a liver-specific promoter. In contrast, we describe here the formation of liver tumors in transgenic mice overexpressing human fibroblast growth factor 19 (FGF19) in skeletal muscle. FGF19 transgenic mice had elevated hepatic alpha-fetoprotein mRNA as early as 2 months of age, and hepatocellular carcinomas were evident by 10 months of age. Increased proliferation of pericentral hepatocytes was demonstrated by 5-bromo-2'-deoxyuridine incorporation in the FGF19 transgenic mice before tumor formation and in nontransgenic mice injected with recombinant FGF19 protein. Areas of small cell dysplasia were initially evident pericentrally, and dysplastic/neoplastic foci throughout the hepatic lobule were glutamine synthetase-positive, suggestive of a pericentral origin. Consistent with chronic activation of the Wingless/Wnt pathway, 44% of the hepatocellular tumors from FGF19 transgenic mice had nuclear staining for beta-catenin. Sequencing of the tumor DNA encoding beta-catenin revealed point mutations that resulted in amino acid substitutions. These findings suggest a previously unknown role for FGF19 in hepatocellular carcinomas.

Spirituality remains part of ongoing explorations in the field of psychology research. Understanding how spirituality informs psychological health can provide a more holistic perspective of the human experience. However, research on the relation between anomalous spiritual experiences and psychological development remains relatively limited. The main objective of this research was to deepen the understanding of spirit communication as an extension of extraordinary human experiences. A qualitative methodology was utilized with a phenomenological type of inquiry to explore lived experiences of spirit communication in a population of evidential mediums. Data were gathered from semi-structured face-to-face interviews, conducted virtually, and analyzed through a lens of transpersonal theory. The analysis revealed several consistent patterns across participants’ accounts. First, their experiences of connecting with a deceased individual were overall emotionally positive experiences. Second, their ability to spiritually connect seemed innate to them (with most participants reporting that mediumship runs in their families). Third, the experience of mediumship was a fluid process, with skills, strengths, and competence in respective states of flux across their lifetimes of experience. When regarded in its entirety, the essence of this phenomenon is a deep connection, akin to emotional intimacy, that provides a sense of closeness, support, and love. The findings suggest that the experience of connecting with deceased individuals is psychologically beneficial as it validates spiritual beliefs regarding the continuation of consciousness beyond the death of the physical body. Furthermore, anomalous spiritual experiences foster a sense of comfort and inquisitiveness as they indicate a reality whose scope extends beyond our conventional understanding.

Determination of the genetically modified organism (GMO) content in foods and feeds by means of real-time polymerase chain reaction (PCR) requires appropriate calibration standards. For this purpose, commercial certified reference materials calibrants (CRMCs) are commonly used. However, quantitative results depend on the actual GMO content of the standards, which may vary from lot to lot. Furthermore, commercial CRMCs are available in the range of 0%-5% only, thus limiting the quantification working range. More recently, recombinant plasmid standards have been used as an alternative to commercial CRMCs. Their production, however, necessitates cloning and microbiology facilities. Here we describe an easy and cost-effective procedure for construction of hybrid amplicon standards containing both transgene and reference gene targets in a tandem orientation on the same molecule. These standards can be rapidly produced in abundant amounts without any equipment for cloning and culturing of recombinant strains. This approach has proved accurate and precise and exhibits major advantages over genomic DNA or plasmids. In this paper we present a validation study for quantitative analysis of Roundup Ready soybean and the 35S-CaMV promoter in genetically modified maize and soybean.[PUBLICATION ABSTRACT]