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37 result(s) for "H. Ghobashy"
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Point of care ultrasonography of quadriceps femoris muscle thickness for assessing nutritional status in critically ill children
Muscle wasting occurs early during critical illness. It is associated with poor PICU outcomes. Ultrasonography can detect muscle thickness in critically ill children. Study quadriceps muscle thickness to assess the nutritional status on admission and follow the muscle thickness change throughout the first week of admission in PICU using point-of-care ultrasonography. This cohort study was conducted on 55 critically ill mechanically ventilated children aged between 2 months and 14 years. Serial measurements of quadriceps muscle thickness were done by ultrasound, during the first week of admission. Quadriceps muscle thickness decreased by 16.4% over the 1st week of admission. The muscle wasting was significantly correlated with STRONGkids score, the inability to achieve target caloric requirements during the 1st week of admission, duration of ventilation, PRISM score, and mortality. Point of care ultrasonography is an easy tool for assessing PICU muscle wasting. Loss of muscle mass during the first week of PICU admission is correlated to mortality, thus it can be utilized in predicting PICU outcomes.
The reliability of POCUS in the diagnosis of community-acquired pneumonia in critically ill pediatric patients: a cross-sectional study
Background Community-acquired pneumonia represents a noteworthy concern in terms of morbidity and mortality, particularly in countries with lower and middle-income levels. Accurate and timely diagnosis of pneumonia is crucial for optimal management. Chest CT is considered the gold standard imaging for diagnosis of pneumonia, but it is not always readily available and exposes children to radiation hazards, so it becomes important to find easily available and less hazardous imaging tools such as lung ultrasound to diagnose pneumonia. A comparative investigation was carried out to assess the diagnostic capability of lung ultrasound in critically ill pediatric patients presenting with respiratory symptoms. Forty-two patients with community-acquired pneumonia from the pediatric intensive care unit were included. Results Lung ultrasound demonstrated high sensitivity (88.1%) and specificity in diagnosing pneumonia, outperforming chest X-ray (50%) and providing comparable results to chest CT (95.2%). In addition, 45.2% of patients required mechanical ventilation, and 69.1% were diagnosed with viral pneumonia. Conclusion The employment of lung ultrasound is deemed secure, accessible, transferable, and efficacious in the expeditious detection of community-acquired pneumonia and the subsequent monitoring of patients. Its high sensitivity and specificity make it a valuable imaging modality in pediatric pneumonia diagnosis, helping clinicians make informed decisions and improve patient outcomes.
oprL Gene Sequencing, Resistance Patterns, Virulence Genes, Quorum Sensing and Antibiotic Resistance Genes of XDR Pseudomonas aeruginosa Isolated from Broiler Chickens
Background: Pseudomonas aeruginosa is incriminated in septicemia, significant economic losses in the poultry production sector, and severe respiratory infections in humans. This study aimed to investigate the occurrence, oprL sequencing, antimicrobial resistance patterns, virulence-determinant, Quorum sensing, and antibiotic resistance genes of P. aeruginosa retrieved from broiler chickens. Methods: Two hundred samples were collected from 120 broiler chickens from broiler farms at Ismailia Governorate, Egypt. Consequently, the bacteriological examination was conducted and the obtained P. aeruginosa strains were tested for oprL gene sequencing, antibiogram, and PCR screening of virulence, Quorum sensing, and antibiotic resistance genes. Results: The overall prevalence of P. aeruginosa in the examined birds was 28.3%. The oprL gene sequence analysis underlined that the tested strain expressed a notable genetic identity with various P. aeruginosa strains isolated from different geographical areas in the USA, India, China, Chile, and Ghana. PCR evidenced that the obtained P. aeruginosa strains, carrying virulence- related genes: oprL, toxA, aprA, phzM, and exoS in a prevalence of 100%, 100%, 42.5%, 33.3%, and 25.9%, respectively. Moreover, the recovered P. aeruginosa strains possessed the Quorum sensing genes: lasI, lasR, rhlI, and rhlR in a prevalence of 85.2%, 85.2%, 81.5%, and 81.5%, respectively. Furthermore, 40.7% of the isolated P. aeruginosa were XDR to seven antimicrobial classes, possessing sul1, [bla.sub.TEM], tetA, [bla.sub.CTX-M], [bla.sub.OXA-1], and aadA1 genes. Conclusion: As we can tell, this is the first report emphasizing the evolution of XDR P. aeruginosa strains from broiler chicken in Egypt, which is supposed to be a serious threat to public health. The emerging XDR P. aeruginosa in poultry frequently harbored the oprL, toxA, and aprA virulence genes, the lasI, lasR, rhlI, and rhlR Quorum sensing genes, and the sul1, [bla.sub.TEM], tetA, [bla.sub.CTXM], [bla.sub.OXA-1], and aadA1 resistance genes. Keywords: P. aeruginosa, MDR, XDR, oprL sequence analysis, Quorum sensing, virulence genes, resistance genes
Gamma irradiation-enhanced performance of waste LLDPE thermally transformed into advanced sponge-like material for oil decontamination
In this study, the development of advanced materials for the removal of oil–water pollution was explored, with a focus on environmental protection. The primary novelty of this research involved the conversion of waste Linear low-density polyethylene (LLDPE) into a sponge-like material denoted as sLLDPE. The process of converting involved thermal treatment in castor oil, resulting in the creation of a porous structure within the material. This sLLDPE material exhibited remarkable oil adsorbent properties and demonstrated enhanced performance in the removal of various organic contaminants from both aqueous and oil-based systems. Furthermore, gamma irradiation-induced crosslinking reactions were implemented within a dose range of 0 up to 90 kGy to further improve its oil removal capabilities. Comparing samples subjected to a radiation dose of 50 kGy with those receiving no irradiation (0 kGy), it was observed that the maximum adsorption capacities for various oils, including crude oil, gasoline oil, motor oil, pump oil, and waste oil, increased significantly. Specifically, the adsorption capacities increased by approximately 216.2%, 235.3%, 24.1%, 111.5%, and 18.6% for the respective oils. It rapidly separated oil–water mixtures with ~ 100% efficiency in a column system and maintained performance over 20 reuse cycles. The converted sLLDPE sponge exhibited excellent organics removal across solvents. The findings of this study not only shed light on the impact of irradiation on polymeric materials but also contribute to our understanding of their potential applications in environmental cleanup processes.
Enhancing corrosion resistance with chemically modified aluminum oxide in UV-curable coatings applied to steel surfaces
This study introduces a novel, environmentally sustainable epoxidized soybean oil acrylate (ESOA) nanocomposite coating containing nAl 2 O 3 -silane nanoparticles (ESOA@TMPTA-nAl 2 O 3 -Silane), which was fabricated using ultraviolet (UV) curing technology. As far as we know, this is the first study to incorporate aluminum oxide nanoparticles (nAl 2 O 3 ) modified through covalent bonding with a reactive diluent monomer, tripropylene glycol diacrylate (TPGDA), and a coupling agent to enhance their dispersibility and interaction within the polymer matrix. Comprehensive characterization techniques, including Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), atomic force microscopy (AFM), X-ray diffraction (XRD), UV-spectroscopy, energy-dispersive X-ray spectroscopy (EDX), and transmission electron microscopy (TEM), confirmed the nanocomposite’s structural and polymer morphological enhancements. Electrochemical impedance spectroscopy (EIS) demonstrated a substantial increase in polarization resistance ( R p ), rising from 25.6 kΩ cm 2 for the unmodified polymer to 288.7 kΩ cm 2 upon the incorporation of (8 wt%) nAl 2 O 3 -Silane. In a similar vein, Potentiodynamic polarization (PDP) exhibited a significant decrease in corrosion current density ( i corr ), diminishing from 0.82 to 0.059 µA/cm 2 , thereby achieving an inhibition efficiency exceeding 99%. Additionally, the salt spray test data showed a considerable improvement in the rust degree from 3 to 8G under identical conditions. The data demonstrates the outstanding corrosion resistance characteristics that the nAl 2 O 3 -Silane nanoparticles provided when coupled with the steel substrate. This improvement is attributed to the excellent dispersion, excellent barrier properties, transparency of the resulting coatings and strong adhesion of nAl 2 O 3 -Silane dispersed in the polymer matrix.
Thermal conversion of irradiated LLDPE waste into sustainable sponge-like compounds: a novel approach for efficient trace-level oil–water removal
The newest method for recycling waste linear low-density polyethylene (LLDPE) is the thermo-catalytic degradation technique known as catalytic pyrolysis. Typically, it is limited by 500–800 °C high temperatures. Catalytic pyrolysis releases toxins and forms harmful carbonized char. The current study is based on exposing wasted LLDPE to different gamma irradiation doses and then pyrolysis in castor oil (150–300 °C). The output product of Ir-(rLLDPE) is turned into another compound with a new structural architecture (sponge-like). SEM analysis confirms conversion, showing sponge-like spicules and layers. Ir-(rLLDPE) is sponge-like with a soft, malleable, absorbent texture. The DSC demonstrates altered thermal properties, with a melting point at 121 °C splitting into two peaks (endothermic at 117 °C and exothermic at 160 °C). The exothermic peaks signify the curing process of the sponge-like material. Ir-(rLLDPE) is assessed as an adsorbent for aqueous oils and solvents. The study examines irradiation doses, pyrolysis temperature, and time on adsorbent capacity. The oil removal obeys the Langmuir isotherm with monolayer adsorption, with a maximum adsorption capacity of 24.75 g/g of waste oil and 43 g/g of 1,1,2,2-tetrachloroethane. Squashing maintains adsorption after 20 reuses. Data shows sponges effectively clean marine oil spills and solvents.
Development of Green and Efficient Extraction Methods of Quercetin from Red Onion Scales Wastes Using Factorial Design for Method Optimization: A Comparative Study
Waste resulting from edible plants is considered one of the best sources of valuable phytochemicals. A promising approach for using these appreciated wastes is extracting precious medically important constituents, for example, free quercetin. Two new cost-effective and green extraction methods are introduced in the present study: ultrasound-assisted glycerol extraction (UAGE) and microwave-assisted extraction (MAE). These extraction protocols are optimized using factorial design to define the highest yield of extraction, and HPLC-UV at 370 nm was used as a method of yield analysis. Quercetin remained stable during the whole process in both extraction protocols. A standard addition technique was performed to quantify quercetin in different extracts and eliminate the matrix effect. In UAGE and MAE, extraction yields were 16.55 ± 0.81 and 27.20 ± 1.55 mg/1g from red onion scales on a dry base, respectively. The amount of quercetin extracted using MAE was superior to UAGE in terms of time and yield. A greenness assessment of the offered studies compared to previously published relevant extraction methods was performed using the analytical eco-scale assessment method (ESA) and national environmental methods index (NEMI). MAE showed to be a greener method with a higher ESA score and a greener NEMI pictogram.