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Background: Citrullus colocynthis L. (C. colocynthis) is commonly known as colocynth. It belongs to the family Cucurbitaceae that is frequently used in alternative medicine in the north of Africa. The aim of the study: the present research was undertaken to investigate the chemical composition, antioxidant, antiproliferative, and antibacterial potentials of C. colocynthis seed extract. Material and methods: the chemical composition of C. colocynthis seed organic extract was characterized using gas chromatography/mass spectrometry (GC-MS). The antioxidant property was carried out using both β-carotene bleaching and DPPH assays. The antibacterial effect was effectuated using the agar disc diffusion method. The antiproliferative activity vs. human colorectal adenocarcinoma cell line (HT-29) and human breast adenocarcinoma cell line (MDA MB 231) were carried by WST-1 test. The chemical analysis showed the presence of interesting potentially bioactive compounds. The studied plant extract exhibited antioxidant potential with IC50 value of 2. 22 mg/mL (β-carotene bleaching) and 8.98 ± 0.619 mg/mL (DPPH). Concerning the antiproliferative activity, the seed extract was effective in MDA-MB-231 and HT-29 cancer cells with IC50 values 86.89 ± 3.395 and 242.1 ± 17.9 μg/mL, respectively, whilst the extract of Citrullus colocynthis seeds was non-toxic in healthy human dermal fibroblasts. Regarding the antibacterial test, the extract was effective in Gram-positive bacteria only. Conclusion: The outcome of this research indicated that the extracts from C. colocynthis seeds may compose a promising source with interesting compounds that can be used to fight cancer, free radicals damage, and bacterial infections.

Introduction and purpose : The prescription of the serum protein profile is useful in the diagnostic orientation of certain immune, inflammatory and nutritional diseases. It is recommended by the senior health authority who defines the conditions. We study the conditions of this prescription by evaluating its relevance in the hospital services of Ibn Rochd University Hospital in Casablanca. Method : a descriptive and cross-sectional study conducted by a questionnaire containing 23 questions, 4 closed questions, 14 multiple-choice questions, 4 closed-ended questions and an open-ended question form 48 doctors. Results of the study : 81.8% of doctors have not been trained in serum protein profile and 72.9% have gaps in their knowledge of the protein profile. 55.7% of doctors say that biological advice is a great contribution. Conclusion : A relevant prescription requires a good enough training and complementarity between clinicians and biologist because of an accelerated diagnosis.

Bis albuminemia is a rare qualitative anomaly, it is hereditary or acquired and transient and physiopathology remains a little unknown. Indeed, the study presents 6 cases of bis albuminemias collected on 2083 serum protein electrophoresis carried out at the laboratory of biochemistry of CHU Ibn Rochd of Casablanca over a spread period of one year (2018). The results of electrophoresis serum proteins of 6 reported cases showed bis albuminemias which are all of acquired and transient type, of which 3 cases are related to the drug intake of betalactamine antibiotic and the other 3 are related to the syndrome nephrotic.

Introduction and purpose: The serum protein profile is a very important examination for the diagnosis, monitoring, surveillance and evaluation of certain inflammatory, immune and nutritional diseases, but its prescription remains a little limited. According to the recommendations of the good practices in biology, the prescription of this examination must be carried out under precise conditions. In our context, this technique is poorly prescribed by physicians. The objective of this work is to assess the state of the art of internal and residents doctors regarding the serum protein profile. Method: This is a descriptive, quantitative, exploratory and cross-sectional study supported by a 22 questions questionnaire, with closed questions in numbers of 4, 14 multiple-choice questions and 4 closed-ended questions from 59 physicians. This questionnaire evaluates their knowledge and their need for serum protein profile training as well as their expectation of benefits at the biochemistry laboratory. Results of the study: The exploitation of the results showed a lack of formation in protein profile. 96.3% of resident and internal pediatric physicians did not receive training in this area. 37.5% have unsatisfactory theoretical knowledge of the targeted protein profile. 40.7% of responders indicated that satisfaction with the biochemistry laboratory's performance is moderately satisfied concerning the response time. Conclusion: Training in protein profile is considered necessary for the prescribing doctors in order to consolidate their knowledge for a more useful prescription for the purpose of an improved diagnosis.