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Reported here are two outbreaks of acute hemorrhagic conjunctivitis that occurred in the Democratic Republic of the Congo and in Morocco in the summers of 2003 and 2004, respectively, with a large impact on public health. Virus was isolated from the conjunctival swabs of 30 Congolese and 20 Moroccan patients. Enterovirus-specific cytopathic effect was observed in all samples. None of the strains could be typed using a conventional neutralization assay with the Melnick intersecting pools; however, by sequencing the VP1 region, the viruses could be identified as coxsackie A24 variants. Phylogenetic analysis of the 3C protease region revealed that these strains were closely related to each other as well as to genotype III isolates detected in Korea in 2002, thus proving their worldwide spread. This is the first report of an epidemic of acute hemorrhagic conjunctivitis due to a coxsackievirus A24 variant in Africa since 1987 and the first ever from Morocco.

The half-a-tetratricopeptide repeat (HAT) motif is a helical repeat motif found in proteins that influence various aspects of RNA metabolism, including rRNA biogenesis, RNA splicing, and polyadenylation. This functional association with RNA suggested that HAT repeat tracts might bind RNA. However, RNA binding activity has not been reported for any HAT repeat tract, and recent literature has emphasized a protein binding role. In this study, we show that a chloroplast-localized HAT protein, HCF107, is a sequence-specific RNA binding protein. HCF107 consists of 11 tandem HAT repeats and short flanking regions that are also predicted to form helical hairpins. The minimal HCF107 binding site spans ∼11 nt, consistent with the possibility that HAT repeats bind RNA through a modular one repeat–1 nt mechanism. Binding of HCF107 to its native RNA ligand in the psbH 5' UTR remodels local RNA structure and protects the adjacent RNA from exonucleases in vitro. These activities can account for the RNA stabilizing, RNA processing, and translational activation functions attributed to HCF107 based on genetic data. We suggest that analogous activities contribute to the functions of HAT domains found in ribonucleoprotein complexes in the nuclear–cytosolic compartment.

This paper focuses on the evaluation of a participatory approach aimed at supporting groups of small-scale farmers in the design of joint drip irrigation projects. Our idea was to create a sustainable social learning environment in which they could acquire adaptive knowledge about new irrigation technology and about designing and managing a joint irrigation project while at the same time improving their negotiation capacities. We developed a framework to evaluate the process as well as the outputs and outcomes of the use of our approach with four groups of smallholder farmers in the Tadla irrigation scheme in Morocco. Our findings showed that the learning environment made it possible to compensate for the knowledge differential among stakeholders and to co-produce knowledge that can be mobilized by small-scale farmers to help them make better informed decisions when choosing whether or not to engage in a joint irrigation project and when developing and implementing such a project. We expect that this will ultimately contribute to supporting the shift from state water to community water through a shared understanding of the technical, economic, and social issues and options related to the management of irrigation water.

The oases of the pre-Saharan basin of Wadi Ferkla in southeastern Morocco receives low and erratic rainfall (annual average of 141 mm and inter-annual standard deviation of 70 mm). From the 1980s, surface water and groundwater are increasingly used due to the expansion of irrigation, mainly along two wadis, namely Wadis Ferkla and Satt originating in the High-Atlas and the Anti-Atlas Mountains, respectively. Their flows reach the Ferkla's irrigated perimeters only when the volume of the flood events exceed upstream evaporation, withdrawals and riverbed's infiltration. Nowadays, these irrigated perimeters exert significant pressure on groundwater resources, through numerous drillings equipped with pumping systems, most of them being powered by solar energy. This increasing water demand situation incentivizes individual farmers to design and implement innovative techniques to increase water access for their farms. For instance, the spreading of floodwaters – an ancestral and collective irrigation practice in traditional oases – is currently being modernized by individual farmers. The new technique consists in partially diverting flood flows into earthen basins. The stored water either infiltrates to recharge local aquifers, or is pumped for flood irrigation of date palms. An experimental protocol was set up to characterize groundwater recharge below one of these on-farm basins equipped with a recharge well. Barometric probes were installed in the basin, in the recharge well and in neighboring boreholes to automatically monitor water levels. A topographic survey of the monitoring points and of the basin aimed at deriving piezometric levels from water levels measurements and estimating the height-surface-volume curves of the basin. After 7 months of continuous monitoring, 3 flood events were recorded. The establishment of the basin water balance at a fine time-resolution allowed estimating its different components including the infiltration rate influencing groundwater recharge. An analytical modeling of this process was developed to assess its effect on groundwater level variations. This approach aims to contribute to a broader reflection on securing water management in this fragile oasis ecosystem.

The Gharb plain in Morocco faces both problems of excess winter rainfall and salinity hazards due to a shallow, permanent and saline groundwater. A large area of 80.000 ha out of a total planned area of 200.000 ha has been equipped with subsurface drains. This system has been designed without any local references and has encountered several maintenance problems, mainly caused by high drain depths. A pilot experiment was installed to provide drainage design criteria appropriate to the local conditions. The main experimental results, based on water and salinity balance and on groundwater flow, are presented in this paper. They show that in the Gharb plain, drainage systems should be designed from winter drainage design criteria. This paper also emphasises that particular attention be paid to surface drainage, which removes about 40% of the excess water.