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Background Update diagnostic methods play essential roles in dealing with the current global malaria situation and decreasing malaria incidence. Aim Global malaria control programs require the availability of adequate laboratory tests in the quick and convenient field. Results There are several methods to find out the existence of parasites within the blood. The oldest one is by microscopy, which is still a gold standard, although rapid diagnostic tests (RDTs) have rapidly become a primary diagnostic test in many endemic areas. Because of microscopy and RDTs limitation, novel serological and molecular methods have been developed. Many kinds of polymerase chain reaction (PCR) provide rapid results and higher specificity and sensitivity. The loop‐mediated isothermal amplification (LAMP) and biosensing‐based molecular techniques as point of care tests (POCT) will become a cost‐effective approach to advance diagnostic testing. Conclusion Despite conventional techniques are still being used in the field, the exploration and field implementation of advanced techniques for the diagnosis of malaria are still being developed rapidly. Update diagnostic methods of malaria play important roles in dealing with the current global malaria situation. There are several methods to find out the existence of parasite within the blood ranging from conventional procedures include light microscopy and RDT till advance procedures include LAMP, PCR, and POCT.

The diagnosis of dengue infection is still a critical factor determining success in the clinical management and treatment of patients. Here, the development of microfluidic paper-based analytical devices (μPADs) utilizing a sandwich immunoassay on wax patterned paper functionalized with anti-dengue NS1 monoclonal antibodies for point-of-care detection of dengue NS1 (DEN-NS1-PAD) is reported. Various assay conditions, including the length of the channel and diluent, were optimized, and the response detected by the naked eye and digitized images within 20–30 min. The DEN-NS1-PAD was successfully tested in the field for detecting dengue NS1 in buffer, cell culture media, and human serum. The limit of detection (LoD) of the DEN-NS1-PAD obtained with the naked eye, scanner, and a smartphone camera was 200, 46.7, and 74.8 ng mL−1, respectively. The repeatability, reproducibility, and stability of the DEN-NS1-PAD were also evaluated. High true specificity and sensitivity in the serum of pediatric patients were observed. These evaluation results confirm that the DEN-NS1-PAD can potentially be used in point-of-care dengue diagnostics, which can significantly impact on the spreading of mosquito-borne diseases, which are likely to become more prevalent with the effects of global warming.

COVID-19 has caused global problems with complex ramifications. Vulnerable households worry about disruptions to food security. Mobility restrictions, decreased salaries, and supply chain disruptions have increased food insecurity. This study examined the best food security model and its determinants. The primary research data were collected from 257 respondents via cluster random sampling. Binary logistic regression, using R-Studio, was employed to analyze the data. The study showed that the Minimal Model (MM) was optimal in explaining food security status, with three predictors: the available food stock (AFS), education of the household head (EHH), and household income (HIc). This aligned with studies showing that food purchase ability depends on income and education. Male household heads demonstrated better food security than females, while women’s education influenced consumption through improved nutritional knowledge. Higher income provides more alternatives for meeting needs, while decreased income limits options. Food reserve storage influenced household food security during the pandemic. The Minimal Model effectively influenced food security through the AFS, EHH, and HIc. The findings underline the importance of available food stock, household head education, and household income in developing approaches to assist food-insecure households. The research makes a significant contribution to ensuring food availability and promoting sustainable development post-pandemic.

The gut microbiota Parvimonas micra has been found to be enriched in gut mucosal tissues and fecal samples of colorectal cancer (CRC) patients compared with non-CRC controls. In the present study, we investigated the tumorigenic potential of P. micra and its regulatory pathways in CRC using HT-29, a low-grade CRC intestinal epithelial cell. For every P. micra-HT-29 interaction assay, HT-29 was co-cultured anaerobically with P. micra at an MOI of 100:1 (bacteria: cells) for 2 h. We found that P. micra increased HT-29 cell proliferation by 38.45% (P=0.008), with the highest wound healing rate at 24 h post-infection (P=0.02). In addition, inflammatory marker expression (IL-5, IL-8, CCL20, and CSF2) was also significantly induced. Shotgun proteomics profiling analysis revealed that P. micra affects the protein expression of HT-29 (157 up-regulated and 214 down-regulated proteins). Up-regulation of PSMB4 protein and its neighbouring subunits revealed association of the ubiquitin–proteasome pathway (UPP) in CRC carcinogenesis; whereas down-regulation of CUL1, YWHAH, and MCM3 signified cell cycle dysregulation. Moreover, 22 clinically relevant epithelial–mesenchymal transition (EMT)-markers were expressed in HT-29 infected with P. micra. Overall, the present study elucidated exacerbated oncogenic properties of P. micra in HT-29 via aberrant cell proliferation, enhanced wound healing, inflammation, up-regulation of UPPs, and activation of EMT pathways.

Septin proteins are a subfamily of closely related GTP-binding proteins conserved in all species except for higher plants and perform essential biological processes. Septins self-assemble into heptameric or octameric complexes and form higher-order structures such as filaments, rings, or gauzes by end-to-end binding. Their close association with cell membrane components makes them central in regulating critical cellular processes. Due to their organisation and properties, septins function as diffusion barriers and are integral in providing scaffolding to support the membrane’s curvature and stability of its components. Septins are also involved in vesicle transport and exocytosis through the plasma membrane by co-localising with exocyst protein complexes. Recently, there have been emerging reports of several human and animal diseases linked to septins and abnormalities in their functions. Most of our understanding of the significance of septins during microbial diseases mainly pertains to their roles in bacterial infections but not viruses. This present review focuses on the known roles of septins in host–viral interactions as detailed by various studies.

This study investigated potentially toxic elements (PTEs) pollution in groundwater across the Maros karst area. The groundwater quality was evaluated using three indexing methods, the heavy metal pollution index (HPI), the heavy metal evaluation index (HMEI) and the contamination index ( The levels of PTEs were calculated to determine the noncancer and cancer risks to the residents through ingestion and dermal adsorption. To obtain high-level accuracy in cancer risk estimation, a Monte Carlo simulation model and sensitivity analysis were performed. The mean values of PTEs in rainy and summer season were followed the order of Cr > Pb > Zn > Cu and Cr > Zn > Pb > Cu, respectively. The high level of Pb and Cr were exceeded the permissible limit of the World Health Organization (WHO) and Indonesian Standards. However, TDS, pH, EC and temperature were still in accordance with WHO guidelines. The geostatistical interpolation of HPI, HMEI and revealed that the groundwater quality around Maros karst is low, particularly in Tukamasea and Leang-Leang village. Hazard index values were lower than one, implying no possibility of noncancer risk. The Monte Carlo simulation results with 95% confidence demonstrated children and adults are at risk for developing cancer due to PTE exposure.

The H5 Avian Influenza A virus infection has emerged as a global concern, particularly in the ASEAN region. This viral infection poses a significant threat to the poultry industry, public health, and regional economies. This region’s reliance on poultry production and the zoonotic potential of H5 subtypes, with documented transmission to various mammalian species and humans, necessitates proactive mitigation strategies. Over the years, comprehensive efforts such as surveillance, vaccination programs, biosecurity measures, and public health education have been implemented to keep outbreaks at bay. In this review, we provide a thorough overview of the H5 infections in the ASEAN region, focusing on the unique challenges and successes in this geographic area. We analyze epidemiological trends, including specific high-risk populations and transmission patterns, and assess the socioeconomic impact of H5 outbreaks on local communities. We also examine regional responses, highlighting innovative surveillance programs, vaccination strategies, and biosecurity measures implemented to control the virus. Furthermore, we explore the crucial role of the One Health approach, emphasizing interdisciplinary collaboration between human, animal, and environmental health sectors. Finally, we discuss future strategies for prevention and control, including the importance of regional cooperation in combating this evolving threat. Through this, we aim to provide valuable insights to the public, policymakers, and researchers involved in tackling H5 infections globally.

The objective of this research was to examine the determinants of farmer participation and shallot-farming development in search of effective farm management practices. The study used structural equation modeling data analysis. The primary data were collected from direct structural interviews with 150 randomly chosen shallot farmers in Bantaeng Regency, Indonesia. It was found that the latent variables of Physical Aspects of Land, System of Economy Peasant Society, and System of Political Peasant Society were fundamental factors that exerted a positive and significant influence on the latent variable of Farmer Participation. Therefore, improvements in the physical aspects of the land, the economic framework, and the political structure of agricultural communities could promote farmer participation. Furthermore, the latent variable of Farmer Participation and System of Political Peasant Society had a positive and significant impact on shallot-farming development. Thus, by increasing the influence of government officials and community leaders, shallot farming can be promoted. The farmers can then enhance their participation in shallot-farming plan formulation and implementation, providing the continued development of shallot farming. The findings of this study contribute significantly to the body of knowledge by validating previous research and proposing different ways to improve effective farm management practices in shallot farming.

Background Diabetic kidney disease populations are categorized as high risk for fasting in Ramadan due to various potential fasting-related complications. Insulin analogues are recommended to be used in place of human insulin during fasting, as they carry a lower risk of hypoglycaemia and stable glycaemic variability. A paucity of data exits on the safety and efficacy of different basal insulin types during fasting for this population. This study aims to evaluate the safety and efficacy of three basal insulin among patients with Type 2 Diabetes Mellitus and concomitant mild to moderate chronic kidney disease who are keen to fast during Ramadan. Materials and methods A single-centered, prospective observational study was conducted among 46 patients with type 2 diabetes mellitus and concomitant chronic kidney disease stage 2 and 3 who were on three different types of basal insulin (Glargine U-100, Levemir, and Insulatard), fasted in Ramadan 2022. All variables were listed as median (IQR). Hypoglycaemia events and glycemic variability obtained from Freestyle Libre continuous glucose monitoring were compared between insulin groups. Changes in glycated haemoglobin, fasting plasma glucose, renal profile, body weight, body mass index, and waist circumference pre and post-Ramadan were evaluated. Results The glycaemic variability was found highest in Insulatard with a median (IQR) of 37.2(33)% versus Levemir 34.4(32.4)% versus Glargine U-100 36.8(30.6)%, p  = NS. Levemir had reported the lowest median time of below range of 2.5(13)% followed by Glargine 4(25)% and Insulatard 5(8)%; p  = NS. The findings of this study indicated that glycated haemoglobin, fasting plasma glucose, renal profile, body weight, body mass index, and waist circumference did not alter statistically between the three groups post-Ramadan. Individually, Insulatard showed a significant reduction in weight and waist circumference (0.9kg, p  = 0.026; 0.44 cm, p  = 0.008) while Levemir showed a reduction in waist circumference (0.75cm, p  = 0.019). Conclusion This study revealed that Insulatard, Levemir, and Glargine demonstrated similar levels of safety and efficacy among those with diabetic kidney disease who observed fasting during Ramadan.

The environmental impact of lime and cement has prompted researchers to seek alternative, eco-friendly, and sustainable construction materials. One approach is to utilize agricultural or industrial waste as a substitute for cement in concrete or soil improvement, thereby reducing waste while providing an eco-friendly and cost-effective solution. Coconut fiber waste and bagasse ash are potential alternatives. This study investigated the effect of a combination of coconut fiber and bagasse ash on improving the properties of soft clay. The coconut fiber content used was 0.75%, while the bagasse ash content varied. The test used was a splitting tensile test, which applied a diametral load to an unconfined compression testing machine. The test results showed that the soil mixed with coconut fiber and bagasse ash experienced a significant increase in tensile strength and more stable lateral deformation. Based on these results, agricultural waste has excellent potential to be developed as a sustainable soil improvement material that minimizes environmental impact.