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Coach-athlete relationship (CAR), thriving and athlete engagement are important psychological variables that affect sports performance. On the basis of self-determination theory, this study constructs a mediation model to examine the influence of CAR on athlete engagement and the mediating effect of thriving between them. This cross-sectional study involves a questionnaire survey with 287 Chinese active athletes (M = 19.63, SD = 2.53) aged 14-26 years (64.5% male, 35.5% female) from eight sports. CAR, thriving and athlete engagement were assessed using the CAR Questionnaire, the Thriving Scale, and the Athlete Engagement Questionnaire, respectively. CAR and its dimensions can significantly and positively predict athlete engagement, complementarity, commitment, and closeness, accounting for 35.1%, 34.6%, and 30.4% of the cumulative variance in dominance analysis, respectively. The direct and indirect paths show that CAR affects athlete engagement through the mediating effect of thriving. The mediating effect model has a good fit and indirect effects account for 56.9% of the total effects. The effect of CAR on athlete engagement reflects a practical application of interpersonal dynamics in competitive sports to a certain extent. The following suggestions can be used to improve athlete engagement. First, setting common goals, emphasizing mutual cooperation, and building trust and support, promote coaches and athletes to have a higher sense of commitment and complementarity to each other, thereby helping improve athlete engagement. Second, meeting the vitality and progress needs of athletes effectively mobilizes CAR resources to promote athlete engagement, which can be manipulated by cultivating closeness, commitment, and complementarity. Third, to ensure the athletes' sports state and mental health, the sports team should focus on the cultivation of athletes' capacities to thrive and internally form a dynamic and positive sports atmosphere in their team. In the future, we can track and compare the influence of the improvement of CAR on thriving and athlete engagement can be tracked and compared from the dual perspectives of coaches and athletes.

Laboratory parameters tended to improve after UC-MSC therapy compared to the status before UC-MSC therapy, including elevated lymphocyte count (median [IQR], 1.09 [0.68–1.35] × 109/L vs 1.43 [1.02–2.20] × 109/L; P <  0.001), decreased C-reactive protein level (median [IQR], 13.39 [1.30–38.86] mg/L vs 0.50 [0.50–6.40] mg/L; P = 0.003), decreased procalcitonin level (median [IQR], 0.07 [0.05–0.09] ng/mL vs 0.04 [0.03–0.06] ng/mL; P <  0.001), decreased interleukin-6 level (median [IQR], 13.78 [5.69–25.26] pg/mL vs 4.86 [2.13–8.19] pg/mL; P <  0.001), decreased D-dimer level (median [IQR], 495 [320–727] ng/mL vs 288 [197–537] ng/mL; P = 0.010), and elevated PaO2/FiO2 (median [IQR], 242 [200–294] vs 332 [288–364]; P <  0.001) (Table 2). [...]days between onset of symptoms and hospital admission were long. A recent report of 7 patients found that bone marrow MSC therapy was an effective treatment for severe COVID-19 [3]. [...]another recent study indicated that bone marrow MSC therapy can improve hypoxia, immune reconstitution, and cytokine storms in patients with severe COVID-19 [4], which was consistent with our results.

Secreted proteins are one of the direct molecular mechanisms by which microbiota influence the host, thus constituting a promising field for drug discovery. Here, through bioinformatics-guided screening of the secretome of clinically established probiotics from Lactobacillus , we identify an uncharacterized secreted protein (named LPH here) that is shared by most of these probiotic strains (8/10) and demonstrate that it protects female mice from colitis in multiple models. Functional studies show that LPH is a bi-functional peptidoglycan hydrolase with both N-Acetyl-β-D-muramidase and DL-endopeptidase activities that can generate muramyl dipeptide (MDP), a NOD2 ligand. Different active site mutants of LPH in combination with Nod2 knockout female mice confirm that LPH exerts anti-colitis effects through MDP-NOD2 signaling. Furthermore, we validate that LPH can also exert protective effects on inflammation-associated colorectal cancer in female mice. Our study reports a probiotic enzyme that enhances NOD2 signaling in vivo in female mice and describes a molecular mechanism that may contribute to the effects of traditional Lactobacillus probiotics. Lactobacillus -based probiotics have been reported to be beneficial for colitis through incompletely understood mechanisms. Here the authors identify an uncharacterized secreted enzyme named LPH from multiple probiotic Lactobacillus strains, which protects female mice from chemically induced colitis and colorectal cancer via NOD2 signalling.

Inflammatory bowel disease (IBD) is a chronic and relapsing intestinal inflammatory condition with no effective treatment. Probiotics have gained wide attention because of their outstanding advantages in intestinal health issues. In previous studies, a novel soluble protein, HM0539, which is derived from GG (LGG), showed significant protective effects against murine colitis, but no clear precise mechanism for this effect was provided. In this study, we hypothesized that the protective function of HM0539 might be derived from its modulation of the TLR4/Myd88/NF-κB axis signaling pathway, which is a critical pathway widely involved in the modulation of inflammatory responses. To test this hypothesis, the underlying anti-inflammatory effects and associated mechanisms of HM0539 were determined both in lipopolysaccharide (LPS)-stimulated RAW 264.7 macrophages and in dextran sulfate sodium (DSS)-induced murine colitis. Our results showed that HM0539 inhibited the expression of cyclooxygenase-2 (COX-2) and the expression inducible nitric oxide synthase (iNOS) by down-regulating the activation of their respective promoter, and as a result this inhibited the production of prostaglandin E2 (PGE2) and nitric oxide (NO). Meanwhile, we demonstrated that HM0539 could ultimately modulate the activation of distal NF-κB by reducing the activation of TLR4 and suppressing the transduction of MyD88. However, even though the overexpression of TLR4 or MyD88 obviously reversed the effect of HM0539 on LPS-induced inflammation, HM0539 still retained some anti-inflammatory activity. Consistent with the findings, we found that HM0539 inhibited to a great extent the production of inflammatory mediators associated with the suppression of the TLR4/Myd88/NF-κB axis activation in colon tissue. In conclusion, HM0539 was shown to be a promising anti-inflammatory agent, at least in part through its down-regulation of the TLR4-MyD88 axis as well as of the downstream MyD88-dependent activated NF-κB signaling, and hence might be considered as a potential therapeutic option for IBD.

Background Depression is recognized as being linked to atherosclerosis and adverse cardiovascular outcomes; however, robust evidence from nationally representative cohorts regarding its association with myocardial infarction (MI) is still insufficient. This study aimed to investigate the relationship between depressive symptoms, measured by the PHQ-9, and the odds of having a history of MI using data from NHANES 2005–2020, while further exploring possible nonlinear patterns and potential modifiers of this association. Methods We included 37,139 adults aged 20 years or older (1,574 with MI) and performed weighted analyses accounting for the NHANES complex survey design. Exposure was defined as PHQ-9 scores, analyzed both continuously and by severity categories (none, mild, moderate, severe), with self-reported MI as the outcome. We fitted three progressively adjusted weighted logistic regression models and employed restricted cubic spline (RCS) analysis to evaluate nonlinearity. We further performed prespecified subgroup and interaction analyses, along with a series of sensitivity analyses. Results Higher depressive burden was significantly associated with higher odds of MI, with MI prevalence rising progressively across depression severity groups (2.89% vs. 4.39% vs. 5.80% vs. 7.08%, p  < 0.001). In the unadjusted model, every 1-point increase in PHQ-9 score corresponded to a 6.2% increase in the odds of MI (OR = 1.062, 95% CI: 1.047–1.077; p  < 0.001), while severe depression was linked to approximately a 2.56-fold higher odds compared with non-depressed participants. The association remained robust after sequential full adjustment for potential confounders. Restricted cubic spline analysis revealed no evidence of nonlinearity ( p  > 0.05), supporting an approximately linear association. Subgroup analyses demonstrated consistent associations across most strata; however, significant interactions were detected for CHF, CHD, and higher V/MPA groups ( P for interaction < 0.05), indicating attenuated associations in these groups. Conclusion In a nationally representative sample of US adults, depressive burden was strongly associated with prevalent MI, with cardiovascular comorbidities and physical activity acting as potential modifiers of this association. These findings underscore the importance of integrating routine depression screening and holistic mind–body management into both general and high-risk populations, and highlight the necessity of prospective and interventional studies to clarify the temporal sequence and evaluate potential causality.

Rubus idaeus is a pivotal cultivated species of raspberry known for its attractive color, distinct flavor, and numerous health benefits. It can be used in pharmaceutical, cosmetics, agriculture and food industries not only as fresh but also as a processed product. Nowadays due to climatic changes, genetic diversity of cultivars has decreased dramatically. However, until now, the status of wild R. idaeus resources in China have not been exploited. In this study, we investigated the resources of wild R. idaeus in China to secure its future potential and sustainability. The MaxEnt model was used to predict R. idaeus suitable habitats and spatial distribution patterns for current and future climate scenarios, based on wild domestic geographic distribution data, current and future climate variables, and topographic variables. The results showed that, mean temperature of the coldest quarter (bio11), precipitation of the coldest quarter (bio19), precipitation of the warmest quarter (bio18), and temperature seasonality (bio4) were crucial factors affecting the distribution of R. idaeus . Presently, the suitable habitats were mainly distributed in the north of China including Xinjiang, Inner Mongolia, Gansu, Ningxia, Shaanxi, Shanxi, Hebei, Beijing, Liaoning, Jilin, Heilongjiang. According to our results, in 2050s, the total suitable habitat area of R. idaeus will increase under SSP1-2.6 and then will be decreased with climate change, while in the 2090s, the total suitable habitat area will continue to decrease. From the present to the 2090s, the centroid distribution of R. idaeus in China will shift towards the east and the species will always be present in Inner Mongolia. Our results provide wild resource information and theoretical reference for the protection and rational utilization of R. idaeus .

http://npic.orst.edu/ingred/ptype/index.html Single cell-inductively coupled plasma-mass spectrometry (SC-ICP-MS) is an emerging technology. In this work, we have developed a novel SC-ICP-MS method to quantify metal ions in individual cells of a toxic cyanobacterial species, Microcystis aeruginosa (M. aeruginosa), without complicated post-dosing sample preparation, and applied this method to study the treatment effectiveness of copper-based algaecides (cupric sulfate and EarthTec®) on the toxic algae M. aeruginosa. The developed SC-ICP-MS method uses new intrinsic metal element magnesium to determine real transport efficiency and cell concentration. The cell viability and microcystin-LR release by algaecide treatment were studied by flow cytometry and ultra-fast liquid chromatography-tandem mass spectrometry, respectively. The results showed that this novel method was very rapid, highly sensitive (detection limits of intracellular copper and magnesium were 65 ag/cell and 98 ag/cell, respectively), and reproducible (relative standard deviation within 12%). The algaecide effectiveness study further demonstrated that copper in the forms of cupric sulfate and copper-based algaecide EarthTec® successfully diminished M. aeruginosa populations. The higher the copper concentration used to treat the cells, the faster the speeds of copper uptake and cell lysis in the copper concentrations ranged from 0 to 200 μg/L of copper-based algaecide. The cells exhibit obvious heterogeneity in copper uptake. The result suggests that M. aeruginosa cells uptake and cumulate copper followed by cellular lysis and microcystin-LR release. These novel results indicated that though the copper-based algaecides could control this type of harmful algal bloom, further treatment to remove the released algal toxin from the treated water would be needed.

ObjectiveO-linked N-acetylglucosaminylation (O-GlcNAcylation), controlled by O-GlcNAcase (OGA) and O-GlcNAc transferase (OGT), is an important post-translational modification of eukaryotic proteins and plays an essential role in regulating gut inflammation. Gut microbiota encode various enzymes involved in O-GlcNAcylation. However, the characteristics, abundance and function of these enzymes are unknown.DesignWe first investigated the structure and taxonomic distribution of bacterial OGAs and OGTs. Then, we performed metagenomic analysis to explore the OGA genes abundance in health samples and different diseases. Finally, we employed in vitro and in vivo experiments to determine the effects and mechanisms of bacterial OGAs to hydrolyse O-GlcNAcylated proteins in host cells and suppress inflammatory response in the gut.ResultsWe found OGAs, instead of OGTs, are enriched in Bacteroidetes and Firmicutes, the major bacterial divisions in the human gut. Most bacterial OGAs are secreted enzymes with the same conserved catalytic domain as human OGAs. A pooled analysis on 1999 metagenomic samples encompassed six diseases revealed that bacterial OGA genes were conserved in healthy human gut with high abundance, and reduced exclusively in ulcerative colitis. In vitro studies showed that bacterial OGAs could hydrolyse O-GlcNAcylated proteins in host cells, including O-GlcNAcylated NF-κB-p65 subunit, which is important for activating NF-κB signalling. In vivo studies demonstrated that gut bacteria-derived OGAs could protect mice from chemically induced colonic inflammation through hydrolysing O-GlcNAcylated proteins.ConclusionOur results reveal a previously unrecognised enzymatic activity by which gut microbiota influence intestinal physiology and highlight bacterial OGAs as a promising therapeutic strategy in colonic inflammation.

Li3V2(PO4)3 (LVP) is one of the candidates for high-energy-density cathode materials matching lithium metal batteries due to its high operating voltage and theoretical capacity. However, the inevitable side reactions of LVP with a traditional liquid-state electrolyte under high voltage, as well as the uncontrollable growth of lithium dendrites, worsen the cycling performance. Herein, a hybrid solid-state electrolyte is prepared by the confinement of a lithium-containing ionic liquid with a mesoporous SiO2 scaffold, and used for a LVP-cathode-based lithium metal battery. The solid-state electrolyte not only exhibits a high ionic conductivity of 3.14 × 10−4 S cm−1 at 30 °C and a wide electrochemical window of about 5 V, but also has good compatibility with the LVP cathode material. Moreover, the cell paired with a solid-state electrolyte exhibits good reversibility and can realize a stable operation at a voltage of up to 4.8 V, and the discharge capacity is well-maintained after 100 cycles, which demonstrates excellent capacity retention. As a contrast, the cell paired with a conventional liquid-state electrolyte shows only an 87.6% discharge capacity retention after 100 cycles. In addition, the effectiveness of a hybrid solid-state electrolyte in suppressing dendritic lithium is demonstrated. The work presents a possible choice for the use of a hybrid solid-state electrolyte compatible with high-performance cathode materials in lithium metal batteries.

The family represents an important subgroup of plant receptor-like kinases (RLKs) that govern growth, signal transduction, reproduction, and stress adaptation. In this study, we performed genome-wide identification, phylogenetic reconstruction, gene structure and motif analysis, evolutionary duplication analysis, promoter cis-element prediction, and tissue- and stress-specific expression profiling of genes. A total of 28 genes were identified and clustered into four well-supported subgroups with conserved structural features. Multiple duplication events, including WGD, TD, PD, DSD, and TRD, contributed to the expansion of this gene family. Promoter analysis revealed abundant cis-elements associated with hormonal regulation, stress responses, and development. Expression analysis showed that /24/25/26 were predominantly expressed in roots, while /24/25 were significantly regulated by drought and salt stress. The expression of specific genes suggests their potential roles in root development. The strong transcriptional responsiveness to abiotic stress indicates that key members may act as critical regulators in sorghum stress tolerance. Collectively, our findings provide a foundation for dissecting the functions of genes in sorghum development and stress adaptation.