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"He, Xiaoqing"
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Core microbes regulate plant-soil resilience by maintaining network resilience during long-term restoration of alpine grasslands
The alpine grasslands of the Qinghai-Tibetan Plateau (QTP), the world’s highest plateau, have been severely degraded. To address this degradation, human-involved restoration efforts, including grassland cultivation, have been implemented. However, the impact of these practices on soil microbial community stability and its relationship with plant-soil system resilience has not been explored. In this study, we evaluate the effects of grassland restoration on microbial communities. We show that bacteria demonstrate higher composition resistance and resilience during the restoration process, when compared to fungi. The changes we observe in microbial community interactions support the stress gradient hypothesis. Our results emphasize the synergistic role of network resilience and the restoration of the plant-soil system. Importantly, we find that core microbial species significantly influence the resilience of the plant-soil system by sustaining the co-occurrence networks. These insights underscore the critical roles of microbial communities in grassland restoration and suggest new strategies for boosting grassland resilience by safeguarding core microbes.
The impacts of alpine grassland restoration on microbial community stability and its relationship with the plant-soil system are unclear. Here, using co-occurrence network analysis, the authors find that core microbes contribute to plant-soil resilience.
Journal Article
Association between sensory impairment and sarcopenia in older Chinese adults: a 4-Year longitudinal study
Objectives
Sarcopenia is a common geriatric syndrome that significantly increases the risk of falls, fractures, disability, and death in older adults. Sensory impairments are also prevalent among the elderly and may exacerbate the decline in physical function, even affecting muscle health. Understanding whether sensory impairments are risk factors affecting sarcopenia in older adults is crucial for developing effective public health policies and intervention strategies. Therefore, this study aims to explore the association between sensory impairments and sarcopenia and its components.
Methods
This study, based on the Chinese Health and Retirement Longitudinal Study (CHARLS), included 4,195 participants aged 60 and above. The assessment of sensory impairment was based on self-reported visual and hearing capabilities. The diagnosis of sarcopenia followed the consensus of the Asian Working Group on Sarcopenia (AWGS) from 2019. Data analysis was conducted using an ordered logistic regression model, and the results report the odds ratios (ORs) and their 95% confidence intervals (CI).
Results
Single sensory impairments at baseline showed no significant correlation with sarcopenia four years later, while dual sensory impairments (DSI) at baseline were significantly associated with sarcopenia (ORs: 1.308, 95% CI: 1.126–1.519). In the analysis of trends over time, transitions from no sensory impairments (NSI) to DSI (ORs: 1.372, 95% CI: 1.028–1.830), from hearing impairments (HI) to DSI (ORs: 1.334, 95% CI: 1.002–1.778), and persistent DSI (ORs: 1.470, 95% CI: 1.159–1.864) were all significantly associated with sarcopenia. Additionally, we found DSI is associated with poor physical performance and muscle mass but not muscle strength.
Conclusions
Our study indicates that DSI have a more severe impact on sarcopenia compared to single sensory impairments. Our findings offer a new perspective for prevention and intervention strategies, suggesting the inclusion of sensory impairment assessments in the clinical evaluation of sarcopenia risk. For elderly individuals with DSI, comprehensive intervention measures should be provided, such as sensory rehabilitation, nutritional support, and guidance on physical activities. For those with only a single sensory impairment, proactive preventive measures should be taken to prevent the progression to DSI.
Journal Article
Room-temperature intrinsic ferromagnetism in epitaxial CrTe2 ultrathin films
While the discovery of two-dimensional (2D) magnets opens the door for fundamental physics and next-generation spintronics, it is technically challenging to achieve the room-temperature ferromagnetic (FM) order in a way compatible with potential device applications. Here, we report the growth and properties of single- and few-layer CrTe
2
, a van der Waals (vdW) material, on bilayer graphene by molecular beam epitaxy (MBE). Intrinsic ferromagnetism with a Curie temperature (
T
C
) up to 300 K, an atomic magnetic moment of ~0.21
μ
B
/Cr and perpendicular magnetic anisotropy (PMA) constant (
K
u
) of 4.89 × 10
5
erg/cm
3
at room temperature in these few-monolayer films have been unambiguously evidenced by superconducting quantum interference device and X-ray magnetic circular dichroism. This intrinsic ferromagnetism has also been identified by the splitting of majority and minority band dispersions with ~0.2 eV at Г point using angle-resolved photoemission spectroscopy. The FM order is preserved with the film thickness down to a monolayer (
T
C
~ 200 K), benefiting from the strong PMA and weak interlayer coupling. The successful MBE growth of 2D FM CrTe
2
films with room-temperature ferromagnetism opens a new avenue for developing large-scale 2D magnet-based spintronics devices.
The emergence of two dimensional ferromagnetism suffers from an inherent fragility to thermal fluctuations, which typically restricts the Curie temperature to below room temperature. Here, Zhang et al present CrTe
2
thin films grown via molecular beam epitaxy with a Curie temperature exceeding 300 K.
Journal Article
Application of AI and deep learning technology for IPE education under dual track cultivation model
This work intends to explore the effectiveness of a dual-track cultivation model for ideological and political literacy in vocational colleges driven by artificial intelligence deep learning models. This work compares the performance of different models on several key indicators of ideological and political education. Through analysis of experimental results across varying data volumes, the optimized model demonstrates significant advantages in four areas: mastery of ideological and political knowledge, ideological and political consciousness, ideological and political practical ability, and student satisfaction. The highest score for political belief reaches 4.8, while the scores for theoretical knowledge mastery, social practice participation, and activity satisfaction all reach 4.7, far surpassing traditional models. This indicates that the optimized model can more effectively help students understand and retain course content. Additionally, the optimized model significantly enhances students’ recognition and trust in the national political system and core values. It also improves students’ ability to apply ideological and political knowledge to real-world problems. Lastly, in terms of student satisfaction, the optimized model performs exceptionally well in both course and activity satisfaction. Therefore, this work contributes to the field of ideological and political education in vocational colleges.
Journal Article
TLR4 Activation Promotes Bone Marrow MSC Proliferation and Osteogenic Differentiation via Wnt3a and Wnt5a Signaling
Mesenchymal stem cells (MSCs) from adult bone marrow maintain their self-renewal ability and the ability to differentiate into osteoblast. Thus, adult bone marrow MSCs play a key role in the regeneration of bone tissue. Previous studies indicated that TLR4 is expressed in MSCs and is critical in regulating the fate decision of MSCs. However, the exact functional role and underlying mechanisms of how TLR4 regulate bone marrow MSC proliferation and differentiation are unclear. Here, we found that activated TLR4 by its ligand LPS promoted the proliferation and osteogenic differentiation of MSCs in vitro. TLR4 activation by LPS also increased cytokine IL-6 and IL-1β production in MSCs. In addition, LPS treatment has no effect on inducing cell death of MSCs. Deletion of TLR4 expression in MSCs completely eliminated the effects of LPS on MSC proliferation, osteogenic differentiation and cytokine production. We also found that the mRNA and protein expression of Wnt3a and Wnt5a, two important factors in regulating MSC fate decision, was upregulated in a TLR4-dependent manner. Silencing Wnt3a with specific siRNA remarkably inhibited TLR4-induced MSC proliferation, while Wnt5a specific siRNA treatment significantly antagonized TLR4-induced MSC osteogenic differentiation. These results together suggested that TLR4 regulates bone marrow MSC proliferation and osteogenic differentiation through Wnt3a and Wnt5a signaling. These finding provide new data to understand the role and the molecular mechanisms of TLR4 in regulating bone marrow MSC functions. These data also provide new insight in developing new therapy in bone regeneration using MSCs by modulating TLR4 and Wnt signaling activity.
Journal Article
Inferring multilayer interactome networks shaping phenotypic plasticity and evolution
Phenotypic plasticity represents a capacity by which the organism changes its phenotypes in response to environmental stimuli. Despite its pivotal role in adaptive evolution, how phenotypic plasticity is genetically controlled remains elusive. Here, we develop a unified framework for coalescing all single nucleotide polymorphisms (SNPs) from a genome-wide association study (GWAS) into a quantitative graph. This framework integrates functional genetic mapping, evolutionary game theory, and predator-prey theory to decompose the net genetic effect of each SNP into its independent and dependent components. The independent effect arises from the intrinsic capacity of a SNP, only expressed when it is in isolation, whereas the dependent effect results from the extrinsic influence of other SNPs. The dependent effect is conceptually beyond the traditional definition of epistasis by not only characterizing the strength of epistasis but also capturing the bi-causality of epistasis and the sign of the causality. We implement functional clustering and variable selection to infer multilayer, sparse, and multiplex interactome networks from any dimension of genetic data. We design and conduct two GWAS experiments using
Staphylococcus aureus
, aimed to test the genetic mechanisms underlying the phenotypic plasticity of this species to vancomycin exposure and
Escherichia coli
coexistence. We reconstruct the two most comprehensive genetic networks for abiotic and biotic phenotypic plasticity. Pathway analysis shows that SNP-SNP epistasis for phenotypic plasticity can be annotated to protein-protein interactions through coding genes. Our model can unveil the regulatory mechanisms of significant loci and excavate missing heritability from some insignificant loci. Our multilayer genetic networks provide a systems tool for dissecting environment-induced evolution.
Genetic plasticity drives phenotypic differences. Here, the authors develop a framework to quantify the individual and combinatorial contributions of SNPs on a phenotype of interest and use it to identify SNP-SNP interactions associated with variations in bacteria’s response to external changes.
Journal Article
Mesothelin promotes epithelial-to-mesenchymal transition and tumorigenicity of human lung cancer and mesothelioma cells
Background
Lung cancer and pleural mesothelioma are two of the most deadly forms of cancer. The prognosis of lung cancer and mesothelioma is extremely poor due to limited treatment modalities and lack of understanding of the disease mechanisms. We have identified mesothelin as a potentially unique therapeutic target that as a specific advantage appears nonessential in most cell types. Mesothelin (MSLN), a plasma membrane differentiation antigen, is expressed at a high level in many human solid tumors, including 70% of lung cancer and nearly all mesotheliomas. However, the role of MSLN in the disease process and underlying mechanisms is largely unknown.
Methods
ShRNA knockdown and overexpression of MSLN were performed in human cancer cell lines and corresponding normal cells, respectively. Tumorigenic and metastatic effects of MSLN were examined by tumor sphere formation, migration, and invasion assays in vitro, as well as xenograft tumor assay in vivo. EMT and CSCs were detected by qPCR array, immunoblotting and flow cytometry.
Results
MSLN plays a key role in controlling epithelial-to-mesenchymal transition (EMT) and stem properties of human lung cancer and mesothelioma cells that control their tumorigenicity and metastatic potential. Firstly, MSLN was found to be highly upregulated in non-small cell lung cancer (NSCLC) patient tissues and in lung carcinoma and mesothelioma cell lines. Secondly, genetic knockdown of MSLN significantly reduced anchorage-independent cell growth, tumor sphere formation, cell adhesion, migration and invasion in vitro, as well as tumor formation and metastasis in vivo. Thirdly, ectopic overexpression of MSLN induced the malignant phenotype of non-cancerous cells, supporting its role as an oncogene. Finally, mechanistic studies revealed that knockdown of MSLN reversed EMT and attenuated stem cell properties, in addition to inhibiting tumor growth and metastasis.
Conclusions
These results indicate an essential role of MSLN in controlling EMT and stem cell properties of human lung cancer and mesothelioma cells. Since EMT is an important process in tumor progression and metastasis, and MSLN is nonessential in most normal tissue, our findings on MSLN may provide new insights into the disease mechanisms and may aid in the development of novel targeted therapy for lung cancer and mesothelioma.
Journal Article
On the effects of carrying capacity and intrinsic growth rate on single and multiple species in spatially heterogeneous environments
We first consider a diffusive logistic model of a single species in a heterogeneous environment, with two parameters, r(x) for intrinsic growth rate and K(x) for carrying capacity. When r(x) and K(x) are proportional, i.e., r=cK, it is proved by Lou (J Differ Equ 223(2):400–426, 2006) that a population diffusing at any rate will reach a higher total equilibrium biomass than the population in an environment in which the same total resources are distributed homogeneously. This paper studies another case when r(x) is a constant, i.e., independent of K(x). In such case, a striking result is that for any dispersal rate, the logistic equation with spatially heterogeneous resources will always support a total population strictly smaller than the total carrying capacity at equilibrium, which is just opposite to the case r=cK. These two cases of single species models also lead to two different forms of Lotka–Volterra competition-diffusion systems. We then examine the consequences of the aforementioned difference on the two forms of competition systems. We find that the outcome of the competition in terms of the dispersal rates and spatial distributions of resources for the two forms of competition systems are again quite different. Our results indicate that in heterogeneous environments, the correlation between r(x) and K(x) has more profound impacts in population ecology than we had previously expected, at least from a mathematical point of view.
Journal Article
Isolation of the Novel Phage PHB09 and Its Potential Use against the Plant Pathogen Pseudomonas syringae pv. actinidiae
Bacteriophages are viruses that specifically infect target bacteria. Recently, bacteriophages have been considered potential biological control agents for bacterial pathogens due to their host specificity. Pseudomonas syringae pv. actinidiae (Psa) is a reemerging pathogen that causes bacterial canker of kiwifruit (Actinidia sp.). The economic impact of this pest and the development of resistance to antibiotics and copper sprays in Psa and other pathovars have led to investigation of alternative management strategies. Phage therapy may be a useful alternative to conventional treatments for controlling Psa infections. Although the efficacy of bacteriophage φ6 was evaluated for the control of Psa, the characteristics of other DNA bacteriophages infecting Psa remain unclear. In this study, the PHB09 lytic bacteriophage specific to Psa was isolated from kiwifruit orchard soil. Extensive host range testing using Psa isolated from kiwifruit orchards and other Pseudomonas strains showed PHB09 has a narrow host range. It remained stable over a wide range of temperatures (4–50 °C) and pH values (pH 3–11) and maintained stability for 50 min under ultraviolet irradiation. Complete genome sequence analysis indicated PHB09 might belong to a new myovirus genus in Caudoviricetes. Its genome contains a total of 94,844 bp and 186 predicted genes associated with phage structure, packaging, host lysis, DNA manipulation, transcription, and additional functions. The isolation and identification of PHB09 enrich the research on Pseudomonas phages and provide a promising biocontrol agent against kiwifruit bacterial canker.
Journal Article