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Goal: To seek and describe current scientific knowledge on the preparedness of medical personnel for an active shooter attack, with the identification of an optimal preparation strategy concerning prevention and potential management of this emergency on the premises of a medical facility.Methods: A scoping study with five phases of methodological steps was applied on the basis of the identified research question. According to the PCC (population, concept, context) format, the research question was divided into individual keywords in selected languages used for the search. Primary studies published between 2000 and December 2023 were included in the selection process. The sources were searched in December 2023 and freely available. Licensed databases EBSCOhost, Cinahl Plus with Full text, Medline, Medline Complete, and Google were used to find relevant studies. We used the \"find similar articles\" function in many databases. Selected English language keywords were searched using Boolean operators and placeholders.Results: The final analysis included nine studies. As precautions, the authors recommended the development of guidelines and establishment of a system, not only at the national level, but also within individual facilities interconnected with the police, regular training of employees with practical simulation, orientation signs in buildings, communication links for quick information and, last but not least, knowledge of warning behaviour.Conclusion: Healthcare providers should be systematically prepared for an active shooter emergency.

Introduction. The disagreement of the general public’s views on home births is practically identical for the professional public and specialists also. The core of the problem lies in the disunity between individual countries of the European Union—complete prohibition under the risk of committing a crime on one side and standard procedure perceived as something completely common on the other side. Methods. The authors focused on the prevalence of home births in individual EU countries, together with the proportion of neonatological mortality compared to the number of live births, which are data that, unlike home births, are mandatory in each EU Member State. Data on home births were obtained from available official and verified sources such as the Ministry of Health, reviews published by the WHO, or published peer-reviewed scientific and professional works. Secondary data were procured via Web of Science, Scopus, or PubMed. Results. The aim of the study was to trace the documented numbers of home births in the individual states of the European Union in the years 2015 to 2019, to analyze them with data on live births together and with data on infant mortality. A comparative analysis of the compiled data can be used to conclude which countries have the highest domestic birth rates and how the birth rate is manifested in these countries. Based on the analysis of available data, it can be determined that the Netherlands, Denmark, and Germany have the highest share of domestic births. The link between home births and increased neonatal mortality has not been established. Eastern Europe countries have the highest neonatal mortality, namely Romania (1.19%) and Malta (0.63%). Conclusion. The Netherlands has the highest domestic birth rate per 100 000 inhabitants with a 5-year average of 161 922 (overall average of all live births 993.40), but is also in 11th place in neonatal mortality, together with Denmark and Belgium, which have 0.35% neonatal neonatal mortality. The country with the lowest neonatal mortality of 0.19% is Slovenia. The total average of all children born in 5 years (915 live births) is 1.422. When monitoring the number of domestic births in other countries in the years 2015 to 2019, an increasing tendency of this trend is observed.

Autor disertační práce se zabývá urgentními příjmy z pohledu nových trendů jejich zabezpečení. Urgentní příjmy jsou specializovanými pracovišti nemocnic a zdravotnických zařízení, které zabezpečují péči o akutně nemocné. Tato oddělení zajišťují nepřetržitou základní diagnostiku a specializovanou oborovou léčbu nemocným v nepřetržitém režimu. Vytváří vstupní bránu nemocnice a jsou tak vystaveny často nepredikovatelným výkyvům počtu příchozích pacientů. Provoz těchto oddělení má tak určitá specifika zabezpečující včasné ošetření pacientů za nejefektivnějšího využití personálu.Urgentní příjmy poskytují nejen péči o akutní stavy za běžných okolností, ale hrají důležitou roli v období krizového provozu, který souvisí s řadou mimořádných událostí. Těmto oddělením se nevyhýbá ani řada nepříznivých situací, které mohou mít negativní dopad na jejich provoz. Proto je snaha veškerá preventivní opatření neustále zefektivňovat.Autor disertační práce se ve své teoretické části věnoval literární rešerši na téma urgentních příjmů, jejich provozu a specifikám. Následně byla posuzována krizová připravenost nemocnice, která má oddělení urgentního příjmu často integrované do svých plánů. Aktuálními tématy v tomto ohledu je v současnosti připravenost zdravotnických zařízení na aktivního střelce a na narušení dodávek elektrické energie. Tyto témata byly hlavní náplní praktické části práce.Prvním vytyčeným cílem bylo stanovit doporučení k přípravě zdravotnického zařízení na aktivního střelce. Tato doporučení se vztahují zejména na urgentní příjmy, které jsou z povahy své struktury a výskytu vysokého počtu pacientů častým cílem těchto incidentů. Doporučení vychází ze scoping review především zahraniční literatury. Druhým cílem bylo stanovit kritické body, které mohou být negativně ovlivněny dlouhodobým výpadkem elektrického proudu. Z pohledu urgentních příjmů se jedná především o omezení diagnostiky a léčby nemocných. Kritickým bodem diagnostiky jsou především metody závislé na stabilitě elektrizační sítě. Touto událostí je však zasažen celý chod nemocnice. Dalším cílem bylo definovat rozdíly v nutnosti zajištění bezpečnostního napájení pro zdravotnické a nezdravotnické prostory při krátkodobém a dlouhodobém výpadku přívodu elektrické energie. Mezitím, co technické standardy reflektují ochranu pacientů proti krátkým výpadkům elektrické energie, opatření pro dlouhodobé výpadky mají jen omezenou možnost plánování. Urgentní příjem je jedno z odděleních, kde se předpokládá funkceschopnost i za takových okolností.Výsledkem práce jsou doporučení zásad prevence a připravenosti na incident s aktivním střelcem. I zde se však setkáváme s některými dilematy, která nejsou v současné literatuře plně zodpovězena. V otázce energetické bezpečnosti byly odhaleny kritické body diagnostiky urgentních stavů za provozu na záložní zdroj elektrické energie a z toho vyplývající rizika pro zdraví a životy pacientů. Obdobně byly z analýzy získány slabá místa, jež jsou podnětem k zamyšlení možnosti rozšíření připravenosti na tyto mimořádné události.

The European Committee for Future Accelerators (ECFA) Early-Career Researcher (ECR) panel, which represents the interests of the ECR community to ECFA, presents in this document its initiatives and activities in the year 2023. This report summarises the process of the first big turnover in the panel composition at the start of 2023 and reports on the activities of the active working groups - either pursued from before or newly established. The overarching goal of the ECFA-ECR panel is to better understand and support the diverse interests of early-career researchers in the ECFA community and beyond.

The 14-3-3 proteins, a family of highly conserved scaffolding proteins ubiquitously expressed in all eukaryotic cells, interact with and regulate the function of several hundreds of partner proteins. Yeast neutral trehalases (Nth), enzymes responsible for the hydrolysis of trehalose to glucose, compared with trehalases from other organisms, possess distinct structure and regulation involving phosphorylation at multiple sites followed by binding to the 14-3-3 protein. Here we report the crystal structures of yeast Nth1 and its complex with Bmh1 (yeast 14-3-3 isoform), which, together with mutational and fluorescence studies, indicate that the binding of Nth1 by 14-3-3 triggers Nth1’s activity by enabling the proper 3D configuration of Nth1’s catalytic and calcium-binding domains relative to each other, thus stabilizing the flexible part of the active site required for catalysis. The presented structure of the Bmh1:Nth1 complex highlights the ability of 14-3-3 to modulate the structure of a multidomain binding partner and to function as an allosteric effector. Furthermore, comparison of the Bmh1:Nth1 complex structure with those of 14-3-3:serotonin N-acetyltransferase and 14-3-3:heat shock protein beta-6 complexes revealed similarities in the 3D structures of bound partner proteins, suggesting the highly conserved nature of 14-3-3 affects the structures of many client proteins.

The original rationale for adopting daylight saving time (DST) was energy savings. Modern research studies, however, question the magnitude and even direction of the effect of DST on electricity consumption. Representing the first meta-analysis in this literature, we collect 162 estimates from 44 studies and find that the mean reported estimate indicates slight electricity savings: 0.34% during the days when DST applies. The literature is not affected by publication bias, but the results vary systematically depending on the exact data and methodology applied. Using Bayesian model averaging we identify the most important factors driving the heterogeneity of the reported effects: data frequency, estimation technique (simulation vs. regression), and, importantly, the latitude of the country considered. Electricity savings are larger for countries farther away from the equator, while subtropical regions consume more electricity because of DST.

Yield is one of the primary concerns for any farmer since it is a key to economic prosperity. Yield productivity zones—that is to say, areas with the same yield level within fields over the long-term—are a form of derived (predicted) data from periodic remote sensing, in this study according to the Enhanced Vegetation Index (EVI). The delineation of yield productivity zones can (a) increase economic prosperity and (b) reduce the environmental burden by employing site-specific crop management practices which implement advanced geospatial technologies that respect soil heterogeneity. This paper presents yield productivity zone identification and computing based on Sentinel-2A/B and Landsat 8 multispectral satellite data and also quantifies the success rate of yield prediction in comparison to the measured yield data. Yield data on spring barley, winter wheat, corn, and oilseed rape were measured with a spatial resolution of up to several meters directly by a CASE IH harvester in the field. The yield data were available from three plots in three years on the Rostěnice Farm in the Czech Republic, with an overall acreage of 176 hectares. The presented yield productivity zones concept was found to be credible for the prediction of yield, including its geospatial variations.

Death-associated protein kinase 2 (DAPK2) is a CaM-regulated Ser/Thr protein kinase, involved in apoptosis, autophagy, granulocyte differentiation and motility regulation, whose activity is controlled by autoinhibition, autophosphorylation, dimerization and interaction with scaffolding proteins 14-3-3. However, the structural basis of 14-3-3-mediated DAPK2 regulation remains unclear. Here, we structurally and biochemically characterize the full-length human DAPK2:14-3-3 complex by combining several biophysical techniques. The results from our X-ray crystallographic analysis revealed that Thr369 phosphorylation at the DAPK2 C terminus creates a high-affinity canonical mode III 14-3-3-binding motif, further enhanced by the diterpene glycoside Fusicoccin A. Moreover, concentration-dependent DAPK2 dimerization is disrupted by Ca2+/CaM binding and stabilized by 14-3-3 binding in solution, thereby protecting the DAPK2 inhibitory autophosphorylation site Ser318 against dephosphorylation and preventing Ca2+/CaM binding. Overall, our findings provide mechanistic insights into 14-3-3-mediated DAPK2 inhibition and highlight the potential of the DAPK2:14-3-3 complex as a target for anti‐inflammatory therapies.Horvath et al. structurally and biochemically characterize the full-length human DAPK2-14-3-3 complex to investigate the effects of binding to DAPK2 on its dimerization, activation by dephosphorylation of Ser318, and Ca2+/calmodulin binding. Their results provide mechanistic insights into 14- 3-3-mediated DAPK2 inhibition and highlight the potential of the DAPK2:14-3-3 complex as a target for anti-inflammatory therapies.

A high density of tumor-infiltrating CD8 + T cells and CD20 + B cells correlates with prolonged survival in patients with a wide variety of human cancers, including high-grade serous ovarian carcinoma (HGSC). However, the potential impact of mature dendritic cells (DCs) in shaping the immune contexture of HGSC, their role in the establishment of T cell-dependent antitumor immunity, and their potential prognostic value for HGSC patients remain unclear. We harnessed immunohistochemical tests and biomolecular analyses to demonstrate that a high density of tumor-infiltrating DC-LAMP + DCs is robustly associated with an immune contexture characterized by T H 1 polarization and cytotoxic activity. We showed that both mature DCs and CD20 + B cells play a critical role in the generation of a clinically-favorable cytotoxic immune response in HGSC microenvironment. In line with this notion, robust tumor infiltration by both DC-LAMP + DCs and CD20 + B cells was associated with most favorable overall survival in two independent cohorts of chemotherapy-naïve HGSC patients. Our findings suggest that the presence of mature, DC-LAMP + DCs in the tumor microenvironment may represent a novel, powerful prognostic biomarker for HGSC patients that reflects the activation of clinically-relevant anticancer immunity.

Yield mapping is a subject of research in (precision) agriculture and one of the primary concerns for farmers as it forms the basis of their income and has implications for subsidies and taxes. The presented approach involves deployment of field harvesters equipped with sensors that provide more detailed and spatially localized values than merely a sum of yields for the whole plot. The measurements from such sensors need to be filtered and subject to further processing, including interpolation, to facilitate follow-up interpretation. This paper aims to identify the relative differences between interpolations from (1) (field) measured data, (2) measured data that were globally filtered, and (3) measured data that were globally and locally filtered. All the measured data were obtained at a fully operational farm and are considered to represent a natural experiment. The revealed spatial patterns and recommendations regarding global and local filtering methods are presented at the end of the paper. Time investments into filtering techniques are also taken into account.