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Many modern medical school curricula have deemphasized the study of anatomy, dedicating significantly less curricular time to student dissection of human bodies and relying instead on other resources, including digital anatomy softwares. Extracurricular student-led dissections allow learners to develop a deeper understanding of the human body, practice surgical skills, and explore clinical interests. We here provide learners and anatomy educators with practical strategies to organize and execute efficient student-led dissections. The authors include senior medical students, anatomy faculty, and clinical instructors at Harvard Medical School with experience in facilitating student-led dissections. Based on our own experience with this approach, and within the background of current developments in anatomy education, we provided ten recommendations for creating a student-led anatomy learning experience. Priorities include understanding student goals, utilizing near-peer teachers, integrating case-based clinical data, partnering with specialty interest groups, and encouraging student independence. Medical students involved in student-led dissections have offered positive feedback with regard to clinical skills, retention of anatomy, and specialty discernment. Future research should gather both students’ subjective perceptions of dissection and objective learning outcomes to quantify the impact of student-led study of anatomy.

Fish are a common cause of allergic reactions associated with food consumption, with parvalbumin being the major allergenic protein. Some fish-hypersensitive patients tolerate some fish species while being allergic to others. Reliable detection methods for allergenic fish species in foods are necessary to ensure compliance with food allergen labeling guidelines to protect fish-allergic consumers. The objective of this project was to develop a multi-analyte detection method for the presence of fish in food. Therefore, conserved parvalbumin exon sequences were utilized for the design of universal PCR primers amplifying intron DNA and small regions of exons flanking the enclosed intron from even very distantly related fish species. An assay for the identification of eight fish species was developed using xMAP™ technology with probes targeting species-specific parvalbumin intron regions. Additionally, a universal fish probe was designed targeting a highly conserved exon region located between the intron and the reverse primer region. The universal fish assay showed no cross-reactivity with other species, such as beef, pork, lamb, chicken, turkey, and shrimp. Importantly, with the exception of one notable case with fish in the same subfamily, species-specific detection showed no cross-reactivity with other fish species. Limits of detection for these eight species were experimentally estimated to range from 0.01% to 0.04%, with potential to increase the detection sensitivity. This report introduces a newly developed method for the multiplex identification of at least eight allergenic fish species in food, which could conceivably be extended to detect up to 100 species simultaneously in one sample. [graphic removed]

Abstract BACKGROUND Pernkopf's atlas of Anatomy contains anatomical plates with detailed images of the peripheral nerves. Its use is controversial due to the author's association with the “Third Reich” and the potential depiction of victims of the Holocaust. The ethical implications of using this atlas for informing surgical planning have not been assessed. OBJECTIVE To (1) assess the role of Pernkopf's atlas in nerve surgeons’ current practice and (2) determine whether a proposal for its ethical handling may provide possible guidance for use in surgery and surgical education. METHODS Members of American Society for Peripheral Nerve and PASSIO Education (video-based learning platform) were surveyed and 182 responses collected. The survey introduced the historical origin of Pernkopf's atlas, and respondents were asked whether they would use the atlas under specific conditions to serve as a recommendation for its ethical handling. An anatomical plate comparison between Netter's and Pernkopf's atlases was performed to compare anatomical accuracy and surgical utility. RESULTS Fifty-nine percent of respondents were aware of Pernkopf's atlas, with 13% currently using it. Aware of the historical facts, 69% were comfortable using the atlas, 15% uncomfortable, and 17% undecided. Additional information on conditions for an ethical approach to the use of the atlas led 76% of those “uncomfortable” and “undecided” to becoming “comfortable” with use. CONCLUSION While the use of Pernkopf's atlas remains controversial, a proposal detailing conditions for an ethical approach in its use provides new guidance in surgical planning and education.

Systematic research on the history of anatomy in National Socialism (NS) has only started in recent years. It has shown that anatomists used the bodies of NS victims for anatomical teaching and research purposes. In this they became complicit with the NS regime. There was a high percentage of Nazi party members among the anatomists, but at the same time there were anatomists whose careers were disrupted for so-called \"racial\" and political reasons. Decisive aspects of this history are first, the fact that traditional sources of anatomical body procurement included increasing numbers of NS victims and second, the gradual change of the traditional anatomical paradigm of working with the dead to a new paradigm of working with the \"future dead\" in human experimentation. This history has importance for the development of new ethical guidelines in anatomy.

We describe the system of public health that evolved in the Vilna Ghetto as an illustrative example of Jewish innovation and achievement during the Holocaust. Furthermore, we argue that by cultivating a sophisticated system of public health, the ghetto inmates enacted a powerful form of Jewish resistance, directly thwarting the intention of the Nazis to eliminate the inhabitants by starvation, epidemic, and exposure. In doing so, we aim to highlight applicable lessons for the broader public health literature. We hope that this unique story may gain its rightful place in the history of public health as an insightful case study of creative and progressive solutions to universal health problems in one of the most challenging environments imaginable.