Explore the vast range of titles available.

We describe the design of the Sunrise Filter Imager (SuFI) and the Image Stabilization and Light Distribution (ISLiD) unit onboard the Sunrise balloon borne solar observatory. This contribution provides the necessary information which is relevant to understand the instruments’ working principles, the relevant technical data, and the necessary information about calibration issues directly related to the science data.

Planktonic copepods are primary consumers in the ocean and are perhaps the most numerous metazoans on earth. Secondary production by these zooplankton supports most food webs of the open sea, directly affecting pelagic fish populations and the biological pump of carbon into the deep ocean. Models of marine ecosystems are quite sensitive to the formulation of the term for zooplankton mortality 1 , 2 , 3 , 4 , although there are few data available to constrain mortality rates in such models. Here we present the first evidence for nonlinear, density-dependent mortality rates of open-ocean zooplankton. A high-frequency time series reveals that per capita mortality rates of eggs of Calanus finmarchicus Gunnerus are a function of the abundance of adult females and juveniles. The temporal dynamics of zooplankton populations can be influenced as much by time-dependent mortality rates as by variations in ‘bottom up’ forcing. The functional form and rates chosen for zooplankton mortality in ecosystem models can alter the balance of pelagic ecosystems 1 , 2 , 3 , modify elemental fluxes into the ocean's interior 5 , and modulate interannual variability in pelagic ecosystems 6 .

Background In view of consistently high cardiovascular morbidity and mortality rates, international efforts are aimed at developing tools for more precise risk prediction to allow preventive treatment targeted at high-risk individuals. Direct visualization of anatomic, preclinical atherosclerotic disease has the potential for individualized risk discrimination. Further, a variety of risk factors are actively evaluated, including markers of the activity of atherosclerotic disease, thrombogenic risk, and genetic polymorphisms. Methods The Heinz Nixdorf RECALL (Risk Factors, Evaluation of Coronary Calcium and Lifestyle) study is a population-based, prospective cohort study of the comparative value of modern risk stratification techniques for “hard” cardiac events. It is designed and powered to define the relative risk associated with the specific extent of coronary atherosclerosis measured by means of electron-beam computed tomography (EBCT)-derived coronary calcium quantities for myocardial infarction and cardiac death in 5 years in 4200 males and females aged 45 to 75 years in an unselected urban population from the large, heavily industrialized Ruhr area. Additionally, the predictive values of conventional cardiovascular risk factors, new candidate and socioeconomic risk factors, certain genetic polymorphisms, and direct signs of subclinical disease are examined with the ankle-brachial index, resting and stress electrocardiograms, and determination of carotid artery intima-media thickness. Prospective clinical risk-benefit and health economic analyses are an inherent part of the study. Study findings with established clinical significance are reported to the participants, but the EBCT findings are withheld until the conclusion of the study. Conclusions The Heinz Nixdorf RECALL study will define appropriate methods for identifying high-risk subgroups in the general urban population who may derive the greatest benefit from preventive treatment. (Am Heart J 2002;144:212-8.)

We describe the design of the Sunrise Filter Imager (SuFI) and the Image Stabilization and Light Distribution (ISLiD) unit onboard the Sunrise balloon borne solar observatory. This contribution provides the necessary information which is relevant to understand the instruments' working principles, the relevant technical data, and the necessary information about calibration issues directly related to the science data.

The third science flight of the balloon-borne solar observatory Sunrise carries three entirely new post-focus science instruments with spectropolarimetric capabilities, concurrently covering an extended spectral range from the near ultraviolet to the near infrared. Sampling a larger height range, from the low photosphere to the chromosphere, with the sub-arcsecond resolution provided by the 1-m Sunrise telescope, is key in understanding critical small-scale phenomena which energetically couple different layers of the solar atmosphere. The Sunrise Ultraviolet Spectropolarimeter and Imager (SUSI) operates between 309 nm and 417 nm. A key feature of SUSI is its capability to record up to several hundred spectral lines simultaneously without the harmful effects of the Earth's atmosphere. The rich SUSI spectra can be exploited in terms of many-line inversions. Another important innovation of the instrument is the synchronized 2D context imaging which allows to numerically correct the spectrograph scans for residual optical aberrations. In this work we describe the main design aspects of SUSI, the instrument characterization and testing, and finally its operation, expected performance and data products.

Blood formation is believed to occur through stepwise progression of haematopoietic stem cells (HSCs) following a tree-like hierarchy of oligo-, bi- and unipotent progenitors. However, this model is based on the analysis of predefined flow-sorted cell populations. Here we integrated flow cytometric, transcriptomic and functional data at single-cell resolution to quantitatively map early differentiation of human HSCs towards lineage commitment. During homeostasis, individual HSCs gradually acquire lineage biases along multiple directions without passing through discrete hierarchically organized progenitor populations. Instead, unilineage-restricted cells emerge directly from a ‘continuum of low-primed undifferentiated haematopoietic stem and progenitor cells’ (CLOUD-HSPCs). Distinct gene expression modules operate in a combinatorial manner to control stemness, early lineage priming and the subsequent progression into all major branches of haematopoiesis. These data reveal a continuous landscape of human steady-state haematopoiesis downstream of HSCs and provide a basis for the understanding of haematopoietic malignancies. Velten et al.  use single-cell transcriptomics and functional data to map the early lineage commitment of human haematopoietic stem cells as a continuous process of cells passing through transitory states rather than demarcating discrete progenitors.

This paper is dedicated to Gerd Döring, who was a great researcher and scientist. He was also an enthusiastic participant of the CF Winter School in Obergurgl/Austria, where the ground for this paper was laid in 2012

Background The gut microbiota has been suggested to play a significant role in the development of overweight and obesity. However, the effects of calorie restriction on gut microbiota of overweight and obese adults, especially over longer durations, are largely unexplored. Methods Here, we longitudinally analyzed the effects of intermittent calorie restriction (ICR) operationalized as the 5:2 diet versus continuous calorie restriction (CCR) on fecal microbiota of 147 overweight or obese adults in a 50-week parallel-arm randomized controlled trial, the HELENA Trial. The primary outcome of the trial was the differential effects of ICR versus CCR on gene expression in subcutaneous adipose tissue. Changes in the gut microbiome, which are the focus of this publication, were defined as exploratory endpoint of the trial. The trial comprised a 12-week intervention period, a 12-week maintenance period, and a final follow-up period of 26 weeks. Results Both diets resulted in ~5% weight loss. However, except for Lactobacillales being enriched after ICR, post-intervention microbiome composition did not significantly differ between groups. Overall weight loss was associated with significant metabolic improvements, but not with changes in the gut microbiome. Nonetheless, the abundance of the Dorea genus at baseline was moderately predictive of subsequent weight loss (AUROC of 0.74 for distinguishing the highest versus lowest weight loss quartiles). Despite the lack of consistent intervention effects on microbiome composition, significant study group-independent co-variation between gut bacterial families and metabolic biomarkers, anthropometric measures, and dietary composition was detectable. Our analysis in particular revealed associations between insulin sensitivity (HOMA-IR) and Akkermansiaceae , Christensenellaceae , and Tanerellaceae . It also suggests the possibility of a beneficial modulation of the latter two intestinal taxa by a diet high in vegetables and fiber, and low in processed meat. Conclusions Overall, our results suggest that the gut microbiome remains stable and highly individual-specific under dietary calorie restriction. Trial registration The trial, including the present microbiome component, was prospectively registered at ClinicalTrials.gov NCT02449148 on May 20, 2015.

We describe the design of the Sunrise Filter Imager (SuFI) and the Image Stabilization and Light Distribution (ISLiD) unit onboard the Sunrise balloon borne solar observatory. This contribution provides the necessary information which is relevant to understand the instruments working principles, the relevant technical data, and the necessary information about calibration issues directly related to the science data.