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In this paper, in-depth research and analysis of juvenile delinquency prevention and occupational therapy education guidance using artificial intelligence are conducted, and its response mechanism is designed in this way. Two crime type prediction algorithms based on time-crime type count vectorization and dense neural network and crime type prediction based on the fusion of dense neural network and long- and short-term memory neural network are proposed. The outputs of both are fed into a new neural network for training to achieve the fusion of the two neural networks. Among them, the use of the dense neural network can effectively fit the relationship between the constructed features and crime types. The behavioral manifestations and causes of the formation of deviant behavior in adolescents are discussed. They can only read numerical data, but there is a lot of information in the textual data that is closely related to the training effect. When experimenting, it is necessary to extract knowledge and build applications. The practical work with adolescents with deviant behaviors is again carried out from group work and casework, respectively, with problem diagnosis, needs assessment, and service plan development for specific clients, to carry out relevant practical service work. The causes of juvenile delinquency in the Internet culture are discussed in terms of the Internet environment, juvenile use of the Internet, Internet supervision, and crime prevention education, respectively. The fourth chapter focuses on the analysis of the prevention and control measures for juvenile delinquency in cyberculture. In response to the above-mentioned causes of juvenile delinquency in cyberculture, the prevention and control measures are discussed in four aspects, namely, strengthening the construction of cyberculture and building a healthy cyber environment, strengthening the capacity building of guiding juveniles to use cyber correctly, building a prevention and supervision system to promote the improvement of the legal system, and improving and innovating the crime prevention education in the cyber era.

Dissipative self-assembly, which requires a continuous supply of fuel to maintain the assembled states far from equilibrium, is the foundation of biological systems. Among a variety of fuels, light, the original fuel of natural dissipative self-assembly, is fundamentally important but remains a challenge to introduce into artificial dissipative self-assemblies. Here, we report an artificial dissipative self-assembly system that is constructed from light-induced amphiphiles. Such dissipative supramolecular assembly is easily performed using protonated sulfonato-merocyanine and chitosan based molecular and macromolecular components in water. Light irradiation induces the assembly of supramolecular nanoparticles, which spontaneously disassemble in the dark due to thermal back relaxation of the molecular switch. Owing to the presence of light-induced amphiphiles and the thermal dissociation mechanism, the lifetimes of these transient supramolecular nanoparticles are highly sensitive to temperature and light power and range from several minutes to hours. By incorporating various fluorophores into transient supramolecular nanoparticles, the processes of aggregation-induced emission and aggregation-caused quenching, along with periodic variations in fluorescent color over time, have been demonstrated. Transient supramolecular assemblies, which act as fluorescence modulators, can also function in human hepatocellular cancer cells. Dissipative self-assembly, which requires a continuous supply of fuel to maintain the assembled states far from equilibrium, is the foundation of biological systems but it remains a challenge to introduce light as fuel into artificial dissipative self-assemblies. Here, the authors report an artificial dissipative self-assembly system that is constructed from light-induced amphiphiles.

The current outbreak of coronavirus disease-2019 (COVID-19) poses unprecedented challenges to global health 1 . The new coronavirus responsible for this outbreak—severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)—shares high sequence identity to SARS-CoV and a bat coronavirus, RaTG13 2 . Although bats may be the reservoir host for a variety of coronaviruses 3 , 4 , it remains unknown whether SARS-CoV-2 has additional host species. Here we show that a coronavirus, which we name pangolin-CoV, isolated from a Malayan pangolin has 100%, 98.6%, 97.8% and 90.7% amino acid identity with SARS-CoV-2 in the E, M, N and S proteins, respectively. In particular, the receptor-binding domain of the S protein of pangolin-CoV is almost identical to that of SARS-CoV-2, with one difference in a noncritical amino acid. Our comparative genomic analysis suggests that SARS-CoV-2 may have originated in the recombination of a virus similar to pangolin-CoV with one similar to RaTG13. Pangolin-CoV was detected in 17 out of the 25 Malayan pangolins that we analysed. Infected pangolins showed clinical signs and histological changes, and circulating antibodies against pangolin-CoV reacted with the S protein of SARS-CoV-2. The isolation of a coronavirus from pangolins that is closely related to SARS-CoV-2 suggests that these animals have the potential to act as an intermediate host of SARS-CoV-2. This newly identified coronavirus from pangolins—the most-trafficked mammal in the illegal wildlife trade—could represent a future threat to public health if wildlife trade is not effectively controlled. A newly identified coronavirus found in Malayan pangolins shares considerable sequence identity with SARS-CoV-2, which suggests that the latter may have originated from a recombination event involving SARS-related coronaviruses from bats and pangolins.

Photocontrollable luminescent molecular switches capable of changing emitting color have been regarded as the ideal integration between intelligent and luminescent materials. A remaining challenge is to combine good luminescence properties with wide range of wavelength transformation, especially when confined in a single molecular system that forms well-defined nanostructures. Here, we report a π-expanded photochromic molecular photoswitch, which allows for the comprehensive achievements including wide emission wavelength variation (240 nm wide, 400–640 nm), high photoisomerization extent (95%), and pure emission color (<100 nm of full width at half maximum). We take the advantageous mechanism of modulating self-assembly and intramolecular charge transfer in the synthesis and construction, and further realize the full color emission by simple photocontrol. Based on this, both photoactivated anti-counterfeiting function and self-erasing photowriting films are achieved of fluorescence. This work will provide insight into the design of intelligent optical materials. Photocontrollable luminescent molecular switches which combine good luminescence properties with a tunable range of wavelength transformation are rare. Here, the authors report a π-expanded photochromic molecular photoswitch which displays wide emission wavelength variation, high photoisomerization extent, and pure emission color.

Macrophage-mediated inflammation plays an important role in hypertensive cardiac remodeling, whereas effective pharmacological treatments targeting cardiac inflammation remain unclear. Lipoprotein-associated phospholipase A2 (Lp-PLA2) contributes to vascular inflammation-related diseases by mediating macrophage migration and activation. Darapladib, the most advanced Lp-PLA2 inhibitor, has been evaluated in phase III trials in atherosclerosis patients. However, the role of darapladib in inhibiting hypertensive cardiac fibrosis remains unknown. Using a murine angiotensin II (Ang II) infusion-induced hypertension model, we found that Pla2g7 (the gene of Lp-PLA2) was the only upregulated PLA2 gene detected in hypertensive cardiac tissue, and it was primarily localized in heart-infiltrating macrophages. As expected, darapladib significantly prevented Ang II-induced cardiac fibrosis, ventricular hypertrophy, and cardiac dysfunction, with potent abatement of macrophage infiltration and inflammatory response. RNA sequencing revealed that darapladib strongly downregulated the expression of genes and signaling pathways related to inflammation, extracellular matrix, and proliferation. Moreover, darapladib substantially reduced the Ang II infusion-induced expression of nucleotide-binding oligomerization domain-like receptor with pyrin domain 3 (NLRP3) and interleukin (IL)-1β and markedly attenuated caspase-1 activation in cardiac tissues. Furthermore, darapladib ameliorated Ang II-stimulated macrophage migration and IL-1β secretion in macrophages by blocking NLRP3 inflammasome activation. Darapladib also effectively blocked macrophage-mediated transformation of fibroblasts into myofibroblasts by inhibiting the activation of the NLRP3 inflammasome in macrophages. Overall, our study identifies a novel anti-inflammatory and anti-cardiac fibrosis role of darapladib in Lp-PLA2 inhibition, elucidating the protective effects of suppressing NLRP3 inflammasome activation. Lp-PLA2 inhibition by darapladib represents a novel therapeutic strategy for hypertensive cardiac damage treatment.

Background Parkinson’s disease (PD) is a neurodegenerative disorder with no absolute cure. The evidence of the involvement of gut microbiota in PD pathogenesis suggests the need to identify certain molecule(s) derived from the gut microbiota, which has the potential to manage PD. Osteocalcin (OCN), an osteoblast-secreted protein, has been shown to modulate brain function. Thus, it is of interest to investigate whether OCN could exert protective effect on PD and, if yes, whether the underlying mechanism lies in the subsequent changes in gut microbiota. Results The intraperitoneal injection of OCN can effectively ameliorate the motor deficits and dopaminergic neuronal loss in a 6-hydroxydopamine-induced PD mouse model. The further antibiotics treatment and fecal microbiota transplantation experiments confirmed that the gut microbiota was required for OCN-induced protection in PD mice. OCN elevated Bacteroidetes and depleted Firmicutes phyla in the gut microbiota of PD mice with elevated potential of microbial propionate production and was confirmed by fecal propionate levels. Two months of orally administered propionate successfully rescued motor deficits and dopaminergic neuronal loss in PD mice. Furthermore, AR420626, the agonist of FFAR3, which is the receptor of propionate, mimicked the neuroprotective effects of propionate and the ablation of enteric neurons blocked the prevention of dopaminergic neuronal loss by propionate in PD mice. Conclusions Together, our results demonstrate that OCN ameliorates motor deficits and dopaminergic neuronal loss in PD mice, modulating gut microbiome and increasing propionate level might be an underlying mechanism responsible for the neuroprotective effects of OCN on PD, and the FFAR3, expressed in enteric nervous system, might be the main action site of propionate. CoKvBsES-WQ_2hkhyhEj9X Video abstract

Determining the one-repetition maximum (1RM) is crucial for organizing training loads, but it also is time-consuming, physically demanding, and poses a risk of injury. Vertical jumps are a less demanding and well-established method to test the ability of the lower limbs to generate great forces over a short time, which may allow for the estimation of 1RM in squatting. The purpose of this study was to develop a model for estimating 1RM back squat from ground reaction forces during vertical jumps. Thirteen healthy participants completed a 1RM back squat test, countermovement jumps, and squat jumps. Five kinematic and kinetic variables (e.g., peak and mean power, relative net impulse, jump height, and peak kinetic energy during various phases) were derived from ground reaction forces collected via a Kistler force plate (1000 Hz). Five out of 5 variables correlated with 1RM in countermovement jump and squat jump (ICC = .96–.98, r = .88–.95, p < .001 and ICC = .97–.99, r = .76–.90, p < .05, respectively). The most accurate stepwise regression model (adjusted R 2 = .90, SEE =  13.24 kg, mean error =  7.4% of mean 1RM m , p < .001) estimated 1RM back squat based on peak kinetic energy during countermovement jumps. Estimation errors ranged from 7.4% to 10.7% of mean measured 1RM, with no differences between estimated and measured values ( d <  0.01, p = .96–1.00). Estimating 1RM via jump tests may offer a practical alternative to traditional methods, reducing injury risks, testing intervals, and effort. Our study proposes a new possible approach for estimating 1RM back squat from jump forces, providing coaches and sports professionals with a more efficient tool to monitor and adjust training loads.

Background To clarify the risk factors for subsidence of titanium mesh cage (TMC) following single-level anterior cervical corpectomy and fusion (ACCF) to reduce subsidence. Methods The present retrospective cohort study included 73 consecutive patients who underwent single-level ACCF. Patients were divided into subsidence ( n  = 31) and non-subsidence groups ( n  = 42). Medical records and radiological parameters such as age, sex, operation level, segmental angle (SA), cervical sagittal angle (CSA), height of anterior (HAE) and posterior endplate (HPE), ratio of anterior (RAE) and posterior endplate (RPE), the alignment of TMC, the global cervical Hounsfield Units (HU) were analyzed. Clinical results were evaluated using the Japanese Orthopedic Association (JOA) scoring system and the Visual Analog Scale (VAS). Results Subsidence occurred in 31 of 73 (42.5%) patients. Comparison between the groups showed significant differences in the value of RAE, the alignment of TMC and the global cervical HU value ( p  < 0.001, p  = 0.002, p  < 0.001). In multivariate logistic regression analysis, RAE > 1.18 (OR = 6.116, 95%CI = 1.613–23.192, p  = 0.008), alignment of TMC > 3° (OR = 5.355, 95%CI = 1.474–19.454, p  = 0.011) and the global cervical HU value< 333 (OR = 11.238, 95%CI = 2.844–44.413, p  = 0.001) were independently associated with subsidence. Linear regression analysis revealed that RAE is significantly positive related to the extent of subsidence (r = − 0.502, p  = 0.006). Conclusion Our findings suggest that the value of RAE more than 1.18, alignment of TMC and poor bone mineral density are the risk factors for subsidence. TMC subsidence does not negatively affect the clinical outcomes after operation. Avoiding over expansion of intervertebral height, optimizing placing of TMC and initiation of anti-osteoporosis treatments 6 months prior to surgery might help surgeons to reduce subsidence after ACCF.

Continuous blood pressure (BP) monitoring is of great significance for the real-time monitoring and early prevention of cardiovascular diseases. Recently, wearable BP monitoring devices have made great progress in the development of daily BP monitoring because they adapt to long-term and high-comfort wear requirements. However, the research and development of wearable continuous BP monitoring devices still face great challenges such as obvious motion noise and slow dynamic response speeds. The pulse wave transit time method which is combined with photoplethysmography (PPG) waves and electrocardiogram (ECG) waves for continuous BP monitoring has received wide attention due to its advantages in terms of excellent dynamic response characteristics and high accuracy. Here, we review the recent state-of-art wearable continuous BP monitoring devices and related technology based on the pulse wave transit time; their measuring principles, design methods, preparation processes, and properties are analyzed in detail. In addition, the potential development directions and challenges of wearable continuous BP monitoring devices based on the pulse wave transit time method are discussed.

The traditional concept of “tonic food” and demand for traditional Chinese medicine make pangolins the largest population of illegally smuggled mammals in the world. Illegal hunting and trade are not only responsible for the sharp decline in pangolin populations but also provide conditions for pathogenic transmission. In 2021, we rescued 21 confiscated unhealthy Malayan pangolins, none of which survived. This study aimed to investigate the reasons for their unexpected deaths and the potential pathogens that may be transmitted during smuggling. Physical examination found that more than 80% pangolins were parasitized with A . javanense ticks. Autopsy and pathological staining analysis revealed multiple organ damage in the deceased pangolins. Pathogens nucleic acid detection of 33 tick samples showed that the positive rate of Rickettsia spp., Anaplasma spp., Ehrlichia spp. Babesia spp., and Colpodella spp. were 90.91%, 6.06%, 6.06%, 15.15% and 18.18%, respectively. Furthermore, pangolin samples were positive for Rickettsia spp. (42.86%, 9/21), Ehrlichia sp. (4.76%, 1/21), and Babesia sp. (4.76%, 1/21). This study confirmed that spotted fever triggered by Rickettsia spp. from A . javanense might accelerate the most death of confiscated pangolins, while Ehrlichia sp., and Babesia sp. infection potentially accelerating a few deaths. Of note, A . javanense ticks carrying Colpodella spp. were detected for the first time in Malayan pangolins. However, whether Colpodella spp. are pathogenic to pangolins is unknown. Further research on the diagnosis, treatment, surveillance, and elimination of ticks and tick-borne diseases in humans, livestock, and wildlife should provide insight into wildlife conservation and zoonotic disease prevention.