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The purplish-red rod-like crystal structure of novel bimetallic metal–organic-frameworks (MOF) CoZn[(4,4′-BDA)(Phen)]2n has been obtained by the reaction of Co2+, Zn2+ (the molar ratio Co: Zn = 1:1), 1,10-phenanthroline (Phen) and 4, 4′-biphenyldicarboxylic acid (4,4′-H2BDA), in which Zn ions of the previously reported monometallic MOF [Zn(4,4′-BDA)(Phen)]2·(HCON(CH3)2n are partly substituted by Co ions. The crystal structure of CoZn[(4,4′-BDA)(Phen)]2n has been characterized by IR, X-ray photoelectron spectroscopy (XPS), and single-crystal X-ray diffraction analysis. Then, the precursors CoZn-MOF-B@C were obtained by adding a surfactant, activated carbon, and NaOH. Its derivative ZnO/CoO@C was used as anode material for lithium-ion batteries, which showed good lithium storage performance. The initial discharge specific capacity of ZnO/CoO@C is 1437.9 mAh g−1 at a current density of 0.2 A g−1, and after 100 cycles, the specific capacity drops to 741.8 mAh g−1.

To explore the clinical value of noninvasive prenatal testing (NIPT) combined with chromosomal microarray analysis (CMA)/copy number variation sequencing (CNV-seq), methylation-specific multiplex ligation-dependent probe amplification (MS-MLPA), and fluorescence in situ hybridization (FISH) in the early screening of imprinted chromosome abnormalities. We retrospectively studied the prenatal diagnosis and pregnancy outcomes of 9 pregnant women with imprinted associated chromosome abnormalities via NIPT. All pregnant women received detailed genetic counselling and prenatal diagnosis. Karyotyping analysis, CNV-seq, CMA, FISH or MS-MLPA were performed on the amniotic fluid samples. We collected the intrauterine phenotypes via ultrasound and followed them up until the induction of labor or one year after birth. Six fetuses (6 out of 9) were diagnosed with regional abnormalities of Imprinting Disease. The most commonly diagnosed syndrome was 15q11-q13 duplication syndrome ( 3 out of 6), followed by mosaic trisomy 7 (2 out of 6) and Temple syndrome (1 out of 6). The other three fetuses (3 out of 9) were diagnosed with absence of heterozygosity (AOH). After genetic counselling, 4 pregnant women (4 out of 9) chose induced labor, 3 pregnant women (3 out of 9) chose spontaneous labor, and 2 pregnant women (2 out of 9) chose cesarean section. The widespread use of NIPT in prenatal screening provides more opportunities to detect rare chromosome aneuploidies (RCAs) and microdeletion/microduplication syndromes (MMSs) in mid-pregnancy. The combination of NIPT and other prenatal diagnostic technologies can help increase the possibility of detecting imprinting-related diseases with no phenotype or a late phenotype in utero.

Despite acknowledging the influence of various lifestyle and metabolic risk factors on hypertension, it remains uncertain to identify the primary contributors and differentiate which modifiable risk factors mediate the causal effects of hypertension. We aimed to examine the hierarchical association of eight prominent lifestyle and metabolic risk factors, along with demographic variables, with hypertension in adults and to explore the mediating effects of modifiable metabolic risk factors on hypertension. A cross-sectional study was conducted in 46 low- and middle-income countries using the World Health Organization (WHO) STEPwise approach to noncommunicable disease risk factor surveillance from 2002 to 2020. In a sample of 179 535 non-pregnant adults, we assessed the weighted population-attributable risk percentages (PAR%) for hypertension associated with eight risk factors. Additionally, we investigated the mediating role of metabolic risk factors on the effects of lifestyle risk factors on hypertension. After adjusting for the sample weight in each country, 26.7% of participants had hypertension. The prevalence of hypertension was highest in those aged ≥65 years, with obesity-associated hypertension (45.7%) exceeding the rates for overweight (32.2%) and non-overweight individuals (18.2%). These eight risk factors collectively explain 83.7% of the PAR% associated with hypertension adjusted for the communal variance. Among the modifiable factors, obesity contributed to a weighted PAR% of 38.2%, while sedentary behaviour and low physical activity combined accounted for a weighted PAR% of 3.1%. Overweight/obesity played a predominant mediating role in the correlation between lifestyle risk factors and systolic and diastolic blood pressure, with the indirect effect accounting for approximately 25-64% and 13-80% of the total effect, respectively. These findings offer new insights into the modified risk factors associated with hypertension in adults in low- and middle-income countries, highlighting the crucial role of maintaining a normal body weight for the effective prevention and management of hypertension.

To evaluate the value of increasing sequencing depths of non-invasive prenatal testing (NIPT) for fetal chromosomal aneuploidies based on the semiconductor sequencing platform. This study recruited a cohort of 59,800 singleton pregnancies from Guangdong Women and Children Hospital between January 2015 and December 2020, including 48,018 cases of NIPT and 11,782 cases of expanded NIPT. Cell-free DNA from plasma samples was sequenced at a sequencing depth of 0.15X for NIPT and 0.4X for expanded NIPT. Patients with positive NIPT results were offered fetal karyotyping or microarray analysis for confirmatory testing, and all pregnant women were followed up. A total of 892 cases were predicted as positive for chromosomal aneuploidies, including 682 cases of NIPT and 210 cases of expanded NIPT, with a positive rate of 1.42% and 1.78% ( p  = 0.0037), respectively. The positive predictive values (PPV) for trisomy 21/18/13 and other autosomal aneuploidies detected by NIPT were 84.80%, 69.23%, 25.00%, and 4.55%, respectively. For expanded NIPT, the positive predictive rates were 86.96%, 80.00%, 35.00%, and 8.77%, respectively. Both NIPT and expanded NIPT have the same PPV for detecting sex chromosome aneuploidies (approximately 50%). Additionally, one false-negative case was identified during the follow-up period. This was a trisomy 18 confirmed by prenatal diagnosis due to multiple ultrasound abnormalities during pregnancy. The study shows that increasing sequencing depth can significantly improve the PPV performance of aneuploidies, with a statistically significant difference, particularly in trisomy 18. The results may provide valuable guidance for clinical doctors during consultations.

It is of great significance to obtain the source of mixed gas emission from the working face and the law of gas emission from each coal seam for the targeted implementation of gas control measures. Based on the principle that the hydrocarbon isotope values of gas in different coal seams have significant variability, a hydrocarbon isotope method for identifying the source of gas emission is proposed. Taking Pingmei No. 6 Coal Mine as the study area, the distribution characteristics of each value were obtained by testing the values of carbon and hydrogen isotopes in the gas of mined coal seams and adjacent coal seams; by testing the hydrocarbon isotope value of CH4 in the mixed gas of coal seam, the proportion of gas emission in each coal seam is determined and the law of gas emission in each coal seam is studied. The results show that the variation law of the proportion of gas emission in each coal seam can be divided into three stages: the dominant stage of gas emission in the mining layer (stage I), the stage of gas emission in the long-distance adjacent coal seam (stage II), and the dynamic equilibrium stage of gas emission in each coal seam (stage III). In the process of working face mining, the amount of gas emission in the mining layer remains in a small fluctuation state, and the proportion of gas emission decreases rapidly in stage I and stage II, and remains stable in stage III; the amount of gas emission and the proportion of gas emission in adjacent coal seams increase rapidly in stage I and stage II, and remain stable in stage III; the mixed gas emission of the working face increases rapidly in stage I and stage II, and remains stable in stage III. The calculation formula of the gas emission rate of the adjacent coal seam is established; during the development of the height of the mining fractured zone, the gas emission rate of the adjacent coal seam increases exponentially, and the gas emission ratio and gas emission amount of the adjacent coal seam increase; after the height of mining fracture zone tends to be stable, the gas emission rate, the proportion of gas emission, and the amount of gas emission remain of adjacent coal seams remain in a small fluctuation state.

Background The identification of cell-free fetal DNA (cffDNA) facilitated non-invasive prenatal screening (NIPS) through analysis of cffDNA in maternal plasma. However, challenges regarding its clinical implementation become apparent. Factors affecting fetal fraction should be clarified to guide its clinical application. Results A total of 13,661 pregnant subjects with singleton pregnancies who undertook NIPS were included in the study. Relationship of gestational age, maternal BMI, and maternal age with the cffDNA fetal fraction in maternal plasmas for NIPS was investigated. Compared with 13 weeks (12.74%) and 14–18 weeks group (12.73%), the fetal fraction in gestational ages of 19–23 weeks, 24–28 weeks, and more than 29 weeks groups significantly increased to 13.11%, 16.14%, and 21.17%, respectively ( P  < 0.01). Compared with fetal fraction of 14.54% in the maternal BMI group of < 18.5 kg/m 2 , the percentage of fetal fraction in the group of 18.5–24.9 kg/m 2 (13.37%), 25–29.9 kg/m 2 (12.20%), 30–34.9 kg/m 2 (11.32%), and 35–39.9 kg/m 2 (11.57%) decreased significantly ( P  < 0.01). Compared with the fetal fraction of 14.38% in the group of 18–24 years old, the fetal fraction in the maternal age group of 25–29 years old group (13.98%) ( P  < 0.05), 30–34 years old group (13.18%) ( P  < 0.01), 35–39 years old group (12.34%) ( P  < 0.01), and ≥ 40 years old (11.90%) group ( P  < 0.01) decreased significantly. Conclusions The percentage of fetal fraction significantly increased with increase of gestational age. Decreased fetal fraction with increasing maternal BMI was found. Maternal age was also negatively related to the fetal fraction.

To determine the association between cell-free DNA fetal fraction (cffDNA) and various prenatal characters to better guide the clinical application of noninvasive prenatal screening (NIPS), a retrospective cohort study of 27,793 women with singleton pregnancies was conducted. Results indicated that no significant difference on cffDNA between trisomy/sex chromosome aneuploidy (SCA) and non-trisomy groups was found. However, the fetal fraction (FF) in the T18 and T13 subgroups were significantly lower than that in the non-trisomy group, while the FF in the T21 group was significantly higher than the non-trisomy group. Pearson’s correlation analysis revealed a positive correlation between √FF and gestational week in the T21, SCA, and non-trisomy groups. A negative correlation between maternal age and √FF in T21 and non-trisomy cases was found, but a positive correlation in SCA group. Compared to the decreasing trend in FF in the T21 group, no significant difference was observed in the SCA group. The √FF level was negatively correlated to maternal BMI in T21 and non-trisomy group, while a positive correlation in SCA group. FF was close related to the result of NIPS and related maternal factors. Though NIPS has increased accuracy, the complexity still should be recognized especially in clinical practice.

This study aimed to evaluate whether the COVID-19 pandemic affected out-of-hospital cardiac arrest (OHCA) care and outcomes across multiple regions in China, despite the country's strict containment measures. Data from the BASeline Investigation of Out-of-hospital Cardiac Arrest (BASIC-OHCA) Utstein Registry between 2019 and 2020 were analyzed. OHCA cases from 18 Emergency Medical Services (EMS) agencies were included. The primary outcome was survival to hospital discharge or 30 days, while secondary outcomes included return of spontaneous circulation (ROSC), and favorable neurologic outcome (Cerebral Performance Category [CPC] score 1–2). Three periods were compared: pre-COVID-19, outbreak, and regular prevention and control. Multilevel logistic regression adjusted for Utstein variables and center-level clustering was used. A total of 16,595 patients received CPR (pre-COVID-19 n = 3890; outbreak n = 5939; regular prevention and control n = 6766). Survival to hospital discharge or 30 days did not differ significantly across periods. ROSC was lower during the outbreak (5.3% vs. 3.6%, AOR 0.80, P = 0.045) and regular prevention and control periods (5.3% vs. 3.5%, AOR 0.80, P = 0.045). Bystander CPR and dispatcher-assisted CPR were significantly less frequent during the regular prevention and control period. EMS response time was longer in the regular prevention and control period (median 12 min vs. 11 min; AOR 1.25, P = 0.006). The COVID-19 pandemic impacted the EMS system in China, reducing bystander CPR, dispatcher-assisted CPR, and ROSC, and increasing EMS response times. However, survival and neurological outcomes did not deteriorate significantly. •This is the first multiregional study compared the outcome of OHCA during the COVID-19 in China.•Survival rates and neurological outcomes remained stable during and after the outbreak of pandemic.•ROSC rates declined during and after the outbreak of pandemic.

Transition metal oxides (TMOs) applied to lithium-ion batteries have attracted increasing attention, but volume expansion during charging and discharging makes their application undesirable. To solve this problem, this paper reports for the first time that core–shell copper-manganese oxide nanoparticles, namely M-CuMn-600, consisting of metal oxides encapsulated in a carbon shell, were obtained by calcining a copper-manganese metal–organic framework (named CuMn-MOF) with pyromellitic acid (PMA) as a ligand at 600℃ under an inert atmosphere for lithium-ion battery applications. The M-CuMn-600 anode material was characterized by X-ray diffraction, field emission scanning electron microscopy (SEM), Brunauer–Emmett–Teller (BET) theory, and X-ray photoelectron spectroscopy (XPS). It has an excellent cycle stability and reversible capacity (709.1 mA h g−1 after 100 cycles at a current density of 200 mA g−1) as an anode material for lithium-ion batteries. The results show that tailoring and optimizing the structure of TMOs is the key to having excellent electrochemical performance.Graphical abstract

Objective To evaluate the performance of noninvasive prenatal testing (NIPT) and NIPT-PLUS for the detection of genome-wide microdeletion and microduplication syndromes (MMSs) at different sequencing depths. The NIPT sequencing depth was 0.15X, and the data volume was 3 million reads; the NIPT-PLUS sequencing depth was 0.4X, and the data volume was 8 million reads. Methods A cohort of 50,679 pregnancies was recruited. A total of 42,969 patients opted for NIPT, and 7710 patients opted for NIPT-PLUS. All high-risk cases were advised to undergo invasive prenatal diagnosis and were followed up. Results A total of 373 cases had a high risk of a copy number variation (CNV) as predicted by NIPT and NIPT-PLUS: NIPT predicted 250 high-risk CNVs and NIPT-PLUS predicted 123. NIPT-PLUS increased the detection rate by 1.02% (0.58% vs 1.60%, p < 0.001). A total of 291 cases accepted noninvasive prenatal diagnosis, with 197 cases of NIPT and 94 cases of NIPT-PLUS. The PPV of CNV > 10 Mb for NIPT-PLUS was significantly higher than that for NIPT ( p = 0.02). The total PPV of NIPT-PLUS was 12.56% higher than that of NIPT (43.61% vs 30.96%, p = 0.03). Conclusion NIPT-PLUS had a better performance in detecting CNVs in terms of the total detection rate and total PPV. However, great care must be taken in presenting results and providing appropriate counseling to patients when deeper sequencing is performed in clinical practice.