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Objectives To screen high active volatile organic compounds (VOCs)-producing Trichoderma isolates against strawberry gray mold caused by Botrytis cinerea, and to explore their antagonistic mode of action against the pathogen. VOCs produced by nine Trichoderma isolates (Trichoderma atroviride T1 and T3; Trichoderma harzianum T2, T4 and T5; T6, T7, T8 and T9 identified as Trichoderma asperellum in this work) significantly inhibited the mycelial growth (13.9−63.0% reduction) and conidial germination (17.6−96.3% reduction) of B. cinerea, the highest inhibition percentage belonged to VOCs of T7; in a closed space, VOCs of T7 shared 76.9% and 100% biocontrol efficacy against gray mold on strawberry fruits and detached leaves, respectively, prolonged the fruit shelf-life by 3 days in presence of B. cinerea, completely protected the leaves from B. cinerea infecting; volatile metabolites of T7 damaged the cell membrane permeability and integrity of B. cinerea, thereby inhibiting the mycelial growth and conidial germination. Gas chromatography-mass spectrometry (GC–MS) analysis revealed the VOCs contain 23 potential compounds, and the majority of these compounds were categorised as alkenes, alcohols, and esters, including PEA and 6PP, which have been reported as substances produced by Trichoderma spp. T. asperellum T7 showed high biofumigant activity against mycelial growth especially conidial germination of B. cinerea and thus protected strawberry fruits and leaves from gray mold, which acted by damaging the pathogen’s plasma membrane and resulting in cytoplasm leakage, was a potential biofumigant for controlling pre- and post-harvest strawberry gray mold.

Black root rot (BRR) caused by Rhizoctonia solani is one of the most destructive soilborne diseases of strawberry worldwide, biological control methods for BRR management are desirable. This work aimed to document antagonistic activity and mechanisms of five Trichoderma isolates ( T . atroviride T1 and T3, T . harzianum T2, T4 and T5) against R. solani in-vitro and in-strawberry-petiole conditions. In-vitro results indicated that all the antagonists shared class 1 of the scale of antagonism, significantly inhibited the pathogen’s sclerotia formation and mycelial growth in dual culture (44–60.9% reduction), in volatile organic compound (VOC) (27.3–75.3% reduction) and in non-VOC assays (13.3–100% reduction). Non-VOCs emitted by T1 or T3 acted by breaking the pathogen’s hypha at the targeted sites of the septum and disorganized the cell structure of the apical hypha, which explained why the mycelia were dissolved in dual culture with T1 or T3. None of the antagonists had the ability to produce siderophore, IAA and hydrogen cyanide. All the antagonists showed good protease-, cellulose-, chitinase- and glucanase-producing ability. However, as mycoparasites of R. solani , higher β-1,3-glucanase-, chitinase- and protease-producing ability of T2 and T5 caused cell wall degradation. In-strawberry-petioles results indicated that antagonisms of the five Trichoderma isolates against R. solani ranked as T2 (34.8%) > T4 (−16.7%) > T3 (−46%) > T5 (−171.5%) > T1 (−172%). In general, the results suggest that the five Trichoderma isolates significantly inhibited R. solani in vitro and shared different strategies against the pathogen . However, except for T2, the other four antagonists significantly increased the severity of petiole necrosis, this might be attributed to the hydrolases produced by the pathogenic and antagonistic fungi synergistically degrading the necrotic petioles.

Knowledge, attitudes and practices (KAP) of the population regarding severe fever with thrombocytopenia syndrome (SFTS) in endemic areas of Lu'an in China were assessed before and after an intervention programme. The pre-intervention phase was conducted using a sample of 425 participants from the 12 selected villages with the highest rates of endemic SFTS infection. A predesigned interview questionnaire was used to assess KAP. Subsequently, an intervention programme was designed and applied in the selected villages. KAP was re-assessed for each population in the selected villages using the same interview questionnaire. Following 2 months of the programme, 339 participants had completed the re-assessed survey. The impact of the intervention programme was evaluated using suitable statistical methods. A significant increase in the KAP and total KAP scores was noted following the intervention programme, whereas the proportion of correct knowledge, the positive attitudes and the effective practices toward SFTS of respondents increased significantly. The intervention programme was effective in improving KAP level of SFTS in populations that were resident in endemic areas.

The East China Sea (ECS) and the South China Sea (SCS) are two major marginal seas of the North Pacific with distinct seasonal variations of primary productivity. Based upon field observations covering both the ECS and the northern SCS (NSCS) during December 2008–January 2009, we examined southward long-range transport of nutrients from the ECS to the northeastern SCS (NESCS) carried by the China Coastal Current (CCC) driven by the prevailing northeast monsoon in wintertime. These escaped nutrients from the ECS shelf, where primary production (PP) was limited in winter, might however refuel the PP on the NESCS shelf at lower latitude, where the water temperature remained favorable, but river-sourced nutrients were limited. By combining the field observation of nitrate+nitrite (NO3+NO2, DIN) with our best estimate of volume transport of the CCC, we derived a first-order estimate for DIN flux of 1430 ± 1024 mol s−1. Under the assumption that DIN was the limiting nutrient, such southward DIN transport would have stimulated 8.84 ± 6.33 × 1011 gC of new production (NP), accounting for 33–74% of the NP or 14–22% of PP in winter on the NESCS shelf shallower than 100 m.

TiO2 has been hampered by drawbacks such as rapid photoelectron and hole recombination and a wide energy band gap of 3.2 eV. In this study, MIL-101(Cr)@TiO2 was synthesised without any mineraliser (HF) as part of material modification approach to overcome those pitfalls. The composite was well characterized by XRD, FT-IR, TEM, XPS, BET, TGA, and Raman spectroscopy. Under optimal synthesis conditions, the 9.17% MIL-101(Cr)@TiO2 composite exhibited 99.9% CBZ degradation after 60 min under UV-A irradiation. This can be attributed to the delayed recombination of photo-generated h+ and e− and a reduced band gap energy of 2.9 eV. A Type II heterojunction structure was proposed for the composite using the Mulligan function of electronegativity with the calculated Ecb and Evb. Besides, trapping experiments and ESR spectroscopy confirmed O2•− as the main ROS for CBZ degradation. The effects of the operating parameters such as pH, UV intensity, composite dosage, and initial pollutant concentration were also evaluated. The scavenging effects of inorganic and organic constituents of pharmaceutical wastewater on the process were also evaluated, with HCO3−, CO32−, and THF having more significant inhibition on the overall CBZ degradation. The degradation pathways of CBZ were also proposed based on detected intermediates with the aid of LC/MS/MS. The composite illustrated reusability and stability without considerable loss in the degradation performance after repeated runs. This work builds on the development of more effective photocatalysts and provides a glimpse into applications for similar MOF heterojunction photocatalysts.

The problem of biological invasions attributed to ballast water release is an ongoing problem that threatens ecosystems and human health. Ultraviolet (UV) radiation has been increasingly used for ballast water treatment mainly due to the advantages of short contact time and minimized harmful disinfection by products. In this study, the impact of salinity on the inactivation of Vibrio cholerae (NCTC 7253) was examined, and comparison of inactivation level and disinfection kinetics after medium-pressure (MP) (1 kW) and low-pressure (LP) (10 W) UV irradiation was made. MP UV exposure resulted in higher inactivation efficacy against V. cholerae than LP UV exposure especially at lower UV doses (≤3 mJ cm−2) and salinity had a negative impact on both MP and LP UV disinfection, especially at higher UV doses (≥3 mJ cm−2 for MP and ≥4 mJ cm−2 for LP). To understand the mechanisms of salinity effect on V. cholerae, the enzyme-linked immunosorbent assay (ELISA) was employed to determine the number of cyclobutane pyrimidine dimers (CPDs), one major type of DNA damage. No significant effects of salinity were found at the CPDs level except for 3% artificial seawater after LP UV exposure case. It is imperative that site-specific conditions of salinity be taken into account in the design of UV reactors to treat V. cholerae and other species.

Probiotics reduce stress-related inflammation and abnormal behaviors in humans and rodents via regulation of the microbiota-gut-brain axis. The objective of this study was to determine if probiotic, Bacillus subtilis, has similar functions in broiler chickens under heat stress (HS). Two hundred forty 1-d-old broiler chicks were assigned to 48 pens with 4 treatments: Thermoneutral (TN)-RD (regular diet), TN-PD (the regular diet mixed with 1 × 106 CFU/g feed probiotic), HS-RD and HS-PD. Probiotic (Sporulin) was fed from day 1; and HS at 32°C for 10 h daily was initiated at day 15. The data showed that final BW, average daily gain , and feed conversion efficiency were improved in PD groups as compared to RD groups regardless of the ambient temperature (P < 0.01). Heterophil to lymphocyte ratio was affected by treatment and its value was in the order of HS-RD > HS-PD > TN-RD > TN-PD birds (P < 0.01). Compared to TN birds, HS birds spent more time in wing spreading, panting, squatting close to the ground, drinking, sleeping, dozing, and sitting but spent less time in eating, standing, and walking (P < 0.05 or 0.01). In addition, HS birds had greater levels of hepatic IL-6, IL-10, heat shock protein (HSP)70, and HSP70 mRNA expression (P < 0.01) and greater levels of cecal IgA and IgY (P < 0.01) compared to TN birds. Within TN groups, TN-PD birds had greater concentrations of hepatic IL-10 (P < 0.05) and cecal IgA (P < 0.01) than TN-RD birds. Within HS groups, HS-PD birds spent less time in wing spreading, panting, squatting close to the ground, drinking, sleeping, dozing, and sitting but spent more time in eating, foraging, standing, and walking than HS-RD birds (P < 0.05 or 0.01). The HS-PD birds also had lower concentrations of hepatic IL-6 and HSP70 (P < 0.01), whereas greater levels of IL-10 (P < 0.05) and lower concentrations of cecal IgA and IgY (P < 0.01). These results indicate that broilers fed the probiotic, B. subtilis, are able to cope with HS more effectively by ameliorating heat-induced behavioral and inflammatory reactions through regulation of microbiota-modulated immunity.

A sensitive and simple method for simultaneous analysis of acetochlor and propisochlor in corn and soil has been developed. Two herbicides were extracted from soil and corn matrices with methanol/water and acetone, respectively, followed by solid phase extraction (SPE) to remove co extractives, prior to analysis by gas chromatography with electron capture detection (GC-ECD). Primary secondary amine (PSA) SPE cartridges (500 mg, 3 mL) were used for sample preparation. The analytes from corn and soil matrices were eluted with 5 mL petroleum ether-acetic ether (95/5, v/v) and 3 mL petroleum ether-acetic ether (95/5, v/v), respectively. The recoveries of two pesticides ranged from 73.8% to 115.5% with relative standard deviations (RSD) less than 11.1% and sensitivity of 0.01 mg/kg, in agreement with directives for method validation in residue analysis. The method was successfully applied to determine the fate of acetochlor and propisochlor in real corn and soil samples. For acetochlor and propisochlor, the half-life times (t1/2) in soil was 5.541 and 6.074 days, respectively. No acetochlor and propisochlor residues (<0.01 mg/kg) were detected in corn at harvest time withholding period of 2.5 months after treatments of the pesticides. Direct confirmation of the analytes in samples was achieved by gas chromatography–mass spectrometry (GC–MS).

Abrupt interdecadal changes in summer precipitation (May – September) over the Indochina Peninsula in the past 40 years have been investigated based on the NCEP-NCAR reanalysis product over 1979–2013 and multiple precipitation datasets. The mechanism for the abrupt change is explored. Results indicate that an abrupt interdecadal change in summer precipitation over the Indochina Peninsula occurred in the middle 1990s, and the annual mean summer precipitation during 1994–2002 increased by about 10% compared to that during 1982–1993. The most significant precipitation change occurred in the central and northern peninsula. Further analysis reveals that the interdecadal decrease in snow cover over the Tibetan Plateau in the winter and spring contributed to the summer precipitation increase over the Indochina Peninsula. The decrease in snow cover over the Tibetan Plateau actually increased the thermal contrast between the Tibetan Plateau and the tropical Indian Ocean-northwestern Pacific, leading to intensified summer monsoon over the northwestern Pacific and the South China Sea. As a result, westerly anomalies occurred from the Bay of Bengal to the northwestern Pacific, while anomalous cyclonic circulation prevailed in the upper levels above East Asia. Correspondingly, the western Pacific subtropical high weakened and shifted eastward. Under the joint effects of the above circulation patterns, the atmosphere became wetter in the Indochina Peninsula and summer precipitation increased. Results of the present study provide a theoretical basis for the prediction of long-term summer precipitation change in the Indochina Peninsula.

•The treatment for recurrent malignant glioma remains to be improved.•Hypofractionated stereotactic radiotherapy is a promising option.•Chemotherapy plus HFSRT may confer a slight survival benefit than HFSRT. Hypofractionated stereotactic radiotherapy (HFSRT) is a common salvage treatment for recurrent malignant glioma (MG). However, it remains controversial whether the combination of HFSRT and chemotherapy could improve survival for patients with recurrent MG compared to HFSRT alone. The present systematic review and meta-analysis aims to investigate this question, and tries to determine to what extent the addition of chemotherapy to HFSRT affects survival. A systematic review was performed to analyse the survival for patients treated with HFSRT combined with chemotherapy or not. Hazard ratios (HRs) with 95% confidence intervals (CIs) for overall survival (OS) were pooled with random effects; and standard mean difference (MD) with 95% CIs for OS were pooled using the same strategy. A total of 7 studies including 388 patients with recurrent MG were eligible for our study. The OS survival of patients receiving combination therapy ranged from 8.7 to 23 months, and the median OS of patients underwent HFSRT ranged from 3.9 to 12 months. The meta-analyses resulted in the pooled HR of 0.44 (95% CI 0.30-0.65, p < 0.0001) (Cochran Q statistic 4.70, P = 0.320, I2 = 14.8%) and pooled standard MD of 0.80 months (95% CI 0.41–1.18, p < 0.001) (Cochran Q statistic 10.16, p = 0.71, I2 = 50.8%). The present study suggests that HFSRT + chemotherapy confers a slight survival improvement for patients with recurrent MG as compared with sole HFSRT management. To draw a more solid conclusion, greater investigation is warranted.