Explore the vast range of titles available.

Background/Aims: Evidence suggests that IL-6 affects bone mass by modulating osteocyte communication towards osteoclasts. However, the mechanism by which IL-6 enhances osteocyte-mediated osteoclastogenesis is unclear. We aimed to investigate the inflammatory factors in serum after orthodontic surgery and their relationship between osteocytes and osteoclasts. Methods: Serum was obtained from 10 orthognathic surgery patients, and inflammatory factors were detected by ELISA. We treated the osteocyte-like cell line MLO-Y4 with recombinant mouse IL-6 and IL-6 receptor (IL-6R), and used quantitative RT-PCR and Western blotting to explore Receptor activator of nuclear factor-κB ligand (RANKL) expression at both the mRNA and protein level. MLO-Y4 cells were co-cultured with osteoclast precursor cells, and the formation of osteoclasts was detected by tartrate-resistant acid phosphatase (TRAP) staining. To explore the role of JAK2 in the osteocyte-mediated osteoclastogenesis, AG490, a JAK2 inhibitor, was used to inhibit the JAK2-STAT3 pathway in osteocytes. Results: In our study, we found that IL-6 and RANKL were stimulated in serum 3-7 days after orthognathic surgery. Therefore, IL-6 and IL-6 receptor enhanced the expression of RANKL at both the mRNA and protein level in MLO-Y4. Furthermore, when MLO-Y4 cells were co-cultured with osteoclast precursor cells, it significantly stimulated osteoclastogenesis. Our study indicated that osteocytes could promote osteoclastic differentiation and the formation of TRAP-positive multinucleated cells after stimulation with IL-6 and IL-6R. Our results also indicated that treatment with IL-6 and IL-6R increased RANKL mRNA expression and the RANKL/OPG expression ratio. Meanwhile, the phosphorylation of Janus kinase 2 (JAK2) and Signal transducer and activator of transcription (STAT3) also correlated with RANKL levels. Furthermore, we investigated the effects of a specific JAK2 inhibitor, AG490, on the expression of RANKL in osteocyte-like MLO-Y4 cells and osteocyte-mediated osteoclastogenesis. The results showed that AG490 inhibited (p)-JAK2 and RANKL expression. Osteoclastic differentiation was decreased after pretreatment in MLO-Y4 with mouse IL-6/IL-6R and AG490; therefore, we concluded that IL-6 increased osteocyte-mediated osteoclastic differentiation by activating JAK2 and RANKL. Conclusion: The effects of IL-6/il-6R and AG490 on osteocyte-mediated osteoclastogenesis contribute to our understanding of the role of inflammatory factors in the interaction between osteocytes and osteoclast precursors. IL-6 and RANKL are key factors for bone remodelling after the orthodontic surgery, and their roles in bone remodelling may be fundamental mechanisms accelerating tooth movement by orthodontic surgery.

Management of infected wounds has raised worldwide concerns. Attempts in this field focus on the development of intelligent patches for improving the wound healing. Here, inspired by the cocktail treatment and combinational therapy stratagem, we present a novel Janus piezoelectric hydrogel patch via 3-dimensional printing for sonodynamic bacteria elimination and wound healing. The top layer of the printed patch was poly(ethylene glycol) diacrylate hydrogel with gold-nanoparticle-decorated tetragonal barium titanate encapsulation, which realizes the ultrasound-triggered release of reactive oxygen species without leaking nanomaterials. The bottom layer is fabricated with methacrylate gelatin and carries growth factors for the cell proliferation and tissue reconstruction. Based on these features, we have demonstrated in vivo that the Janus piezoelectric hydrogel patch can exert substantial infection elimination activity under the excitation of ultrasound, and its sustained release of growth factors can promote tissue regeneration during wound management. These results indicated that the proposed Janus piezoelectric hydrogel patch had practical significance in sonodynamic infection alleviation and programmable wound healing for treating different clinical diseases.

Revealing the synoptic patterns related to hourly extreme precipitation (EP) is very important to deepen our recognition and understanding of EP formation. The predominant synoptic patterns associated with the summer regional hourly EP events (RHEPE) over the central‐eastern Tibetan Plateau (CETP) and Sichuan Basin (SCB) have been systematically identified. Results show the summer RHEPE over CETP and SCB are dominated by the background large‐scale circulations featured by the configuration of eastward‐extended South Asia high (SAH) and westward‐extended Western North Pacific Subtropical High (WNPSH) and their northward advance, except for that an obvious low‐level vortex imbedded in the background large‐scale circulations is mainly responsible for the summer RHEPE over SCB. The frequency and intensity of the total summer RHEPE over CETP and SCB all show an obvious increasing trend during 2000–2020, which is largely contributed by the synoptic pattern characterized by the configuration of eastward extended SAH and westward extended WNPSH. Plain Language Summary To completely indicate the synoptic patterns leading to the summer regional hourly extreme precipitation events (RHEPE) over the central‐eastern Tibetan Plateau (CETP) and Sichuan Basin (SCB), this study has identified the dominant synoptic patterns of summer RHEPE over the two regions. Results show that the configuration of eastward extended South Asia high (SAH) and westward extended Western North Pacific Subtropical High (WNPSH) and their northward advance dominates the prime circulation patterns of summer RHEPE over CETP and SCB. However, in addition to the opposing motion of the SAH and WNPSH, a low‐level vortex imbedded in the background large‐scale circulations is mainly responsible for the summer RHEPE over SCB. The findings of this study may help us to deepen our understanding of the RHEPE formation over the two regions with complex terrain and provide a base to further improve the prediction of extreme precipitation. Key Points Summer rainfall extremes over both central‐eastern Tibetan Plateau (CETP) and Sichuan Basin (SCB) are dominated by the combination of eastward extended SAH and westward extended Western North Pacific Subtropical High The summer rainfall extremes over SCB are mainly induced by a low‐level vortex imbedded in the background of large‐scale circulations The dominant synoptic patterns over CETP and SCB all contribute to the increasing trends of frequency and intensity of the summer regional hourly EP events

Based on the hourly rainfall gauge data and ERA5 reanalysis for the period 1980–2020, typical synoptic patterns responsible for summer regional hourly extreme precipitation events (RHEPE) over the middle and lower Yangtze River basin have been objectively identified using a circulation clustering method. It is found that the Meiyu front with different locations and intensities imbedded in the East Asian summer monsoon, and landfalling typhoons are the leading contributors. As the dominant synoptic pattern, the Meiyu front pattern is associated with ∼92% of the total RHEPE occurrence and can be categorized into a southerly strong‐Meiyu type and a northerly weak‐Meiyu type. The RHEPE occurrence shows a predominant morning peak associated with the southerly strong‐Meiyu type and a secondary late afternoon peak related to the northerly weak‐Meiyu type, in which the Meiyu front is pushed northward by the strengthened western North Pacific subtropical high accompanied by accelerated low‐level southwesterly flow. Plain Language Summary Using ERA5 reanalysis and hourly gauge rainfall measurements in summers of 1980–2020, this study investigates the driving mechanisms and temporal variation of summer regional hourly extreme rainfall events over the middle and lower Yangtze River basin. Typical synoptic patterns responsible for the summer regional hourly rainfall extremes are objectively identified using spectral clustering analysis. The Meiyu front with different locations and intensities imbedded in East Asian summer monsoon and the landfalling typhoons are the synoptic patterns leading to the regional hourly rainfall extremes. The diurnal twin peaks (morning and late afternoon) in the occurrence of regional hourly rainfall extremes are related to the Meiyu front with different locations and intensities. The results from this investigation may help improve the prediction and climate risk assessment of regional extreme rainfall events. Key Points The Meiyu front imbedded in the East Asian summer monsoon and landfalling typhoons are the leading contributors of summer regional hourly extreme precipitation events (RHEPE) over middle and lower Yangtze River basin The Meiyu front pattern can be sorted into a southerly type with strong Meiyu front and a northerly type with weak Meiyu front The northerly weak Meiyu front pattern with active convection contributes the most to the afternoon diurnal peak of RHEPE occurrence

Antibiotics provide promising strategies for treating periodontitis, while their delivery and controllable release with desired oral retention remain challenging. Here, inspired by the unique suction‐cup structures of abalones, a novel adhesive and photo‐responsive microparticle (MP) delivery system is developed to treat periodontitis through microfluidic electrospray technology. Such MPs are generated by quickly ionic cross‐linking of sodium alginate together with photo‐curing of poly(ethylene glycol) diacrylate of the distorted microfluidic droplets during their high‐speed dropping into calcium chloride solution. Attributing to their unique concave structures, the abalone‐inspired MPs exhibit desired underwater adhesion ability and stability under running water. In addition, due to the loading of antibiotics minocycline hydrochloride and near‐infrared (NIR)‐responsive black phosphorus during their fabrication, the resultant MPs can not only eradicate bacteria directly, but also realize a controllable and effective drug release upon NIR irradiation. Based on these features, it is demonstrated from in vivo periodontitis that the abalone‐inspired MPs are firmly adhesive and can controlled‐release drugs on the tooth, and thus have outstanding antibacterial efficacy against Porphyromonas gingivalis. These results indicate the particular values of the abalone‐inspired MPs for oral‐related disease treatment. By encapsulating the black phosphorus and minocycline hydrochloride, the abalone‐inspired microparticles with the enhanced adhesive ability and controllable drug release capacity can realize antibacterial photothermal therapy on the tooth.

Micro‐ and nanorobots (MNRs) propelled by external actuations have broad potential in biomedical applications. Among the numerous external excitations, ultrasound (US) features outstanding practical significance with merits of its noninvasiveness, tunability, penetrability, and biocompatibility. Attributing to various physiochemical effects of US, it can propel the MNRs with sophisticated structures through asymmetric acoustic streaming, bubble oscillation, and so on. In this review, we introduce several advanced and representative US‐propelled MNRs with inhomogeneous density distribution, asymmetric shape, hollow cavity, etc. The potential biomedical applications of these cutting‐edge MNRs are also presented, including intracellular delivery, harmful substances collection, and so on. Furthermore, we conclude the advantages and limitations of US‐propelled MNRs and prospect their future developments in multidisciplinary fields. Ultrasound (US)‐propelled micro‐ and nanorobots (MNRs) have shown their bright future in multidisciplinary fields. In this review, several advanced and representative US‐propelled MNRs with inhomogeneous density distribution, asymmetric shape, hollow cavity, and other features are presented. The potential biomedical applications of these cutting‐edge MNRs are also introduced, including intracellular delivery, harmful substances collection, etc. Finally, the advantages and limitations of US‐propelled MNRs are concluded, and their future developments in multidisciplinary fields are also prospected.

In East Asia, the climate variability in boreal winter is dominated by the East Asian winter monsoon, one of the most energetic monsoon systems that can lead to disasters. The key variable, the East Asian winter surface air temperature (SAT), has significantly changed over the past century and has substantially impacted agriculture, ecosystems, economics, and public health. However, its projections are limited by considerable uncertainties. Here, we identify the first leading mode that explains almost 29.6% of the inter-model spread in future SAT change. Our research delves into the evolution of present-day biases under future scenarios and their consequential impact on the SAT. Models with stronger western currents’ heat transport in the North Pacific exhibit a warmer North Pacific at mid-latitudes during historical periods. Additionally, these models consistently demonstrate stronger western currents in the future, contributing to the amplified warming of the western North Pacific, thereby warming Eurasia via the weakened trough and subtropical jet through barotropic responses to the warm North Pacific. Incorporating observational sea surface temperature constraints reduces uncertainties by 9.40%, revealing a more reliable SAT change pattern by the end of the 21st century.

The global capacity for wind power has grown rapidly in recent years, yet uncertainties in wind power density (WPD) assessments still hinder effective climate change mitigation efforts. One major challenge is the significant underestimation of WPD when using coarser temporal resolutions (∆t) of wind speed data. Here, we show that using daily ∆t results in an average underestimation of 35.6% in global onshore WPD compared to hourly ∆t. This discrepancy arises from the exponential decay of WPD with increasing ∆t, reflecting the intrinsic properties of wind speed distributions, particularly in regions with weaker winds. To address this, we propose a calibration method that introduces a correction coefficient to reduce biases and harmonize WPD estimates across temporal resolutions. Applying this method to future wind energy projections under the Shared Socioeconomic Pathway 585 scenario increases global onshore WPD estimates by 25% by 2100, compared to uncorrected daily data. These findings highlight the effectiveness of calibration in reducing uncertainties, enhancing WPD assessments, and facilitating robust policy action toward carbon neutrality. This study shows that using wind speed data with coarser temporal resolution significantly underestimates global wind power density (WPD). A calibration method increases future global onshore WPD estimates by 25% compared to uncorrected daily data.

Exosomes-loaded hydrogels have potential value in wound treatment. Current studies focus on improving hydrogels’ biocompatibility and optimizing different stem cell-derived exosomes for better therapeutic effect. Herein, we present a novel biocompatible recombinant human collagen (RHC) hydrogel loading with different MSCs-derived exosomes for promoting wound healing. We modify the RHC with methacrylate anhydride (MA) at optimal concentration, generating collagen hydrogel (RHCMA) with ideal physiochemical properties for exosome delivery (MSC-exos@RHCMA). Exosomes derived from human adipose-derived MSCs (ADSC-exos), bone marrow-derived MSCs (BMSC-exos) and umbilical cord MSCs (ucMSC-exos) are harvested from the culture supernatants and are loaded into RHCMA, respectively. These three hydrogel systems exhibit desired sustained release features, and can significantly improve cell proliferation and migration. In addition, these MSC-exos@RHCMAs show excellent therapeutic performance in treating the wounds of rats. Notably, we have demonstrated that the healing effect occurs best under the treatment of ucMSC-exos@RHCMA, following inflammatory resolution, angiogenesis, and collagen formation. These results would supply important value for the clinical application of MSC-exos in wound treatment in the future.

Mesenchymal stem cell (MSC) therapy is a promising candidate for inflammatory bowel disease (IBD) treatment, while overcoming the limitations of naive seeding cells function and realizing efficient intestinal targeting remains a challenge. Here, a bioadhesive microparticle carrying interleukin‐27 (IL‐27) MSC‐derived extracellular vesicles (MSC IL‐27 EVs) is developed to treat IBD. The MSC IL‐27 EVs prepared through lentivirus‐mediated gene transfection technology show ideal anti‐inflammatory and damage repair function. By encapsulating MSC IL‐27 EVs into dopamine methacrylamide‐modified hydrogel, a bioadhesive EVs microcarrier via microfluidic technology is fabricated. The resultant microcarriers exhibit ideal MSC IL‐27 EVs sustained release effect and effective wet adhesion property. Furthermore, the therapeutic potential of MSC IL‐27 EVs‐loaded microcarriers in treating IBD is demonstrated. Through giving IBD rats a rectal administration, it is found that the microcarriers can firmly anchor to the surface of colon, reduce the inflammatory response, and repair the damaged barrier. Therefore, the bioadhesive MSC IL‐27 EVs‐loaded microcarriers provide a promising strategy for the biomedical application of MSC‐derived EVs, and broaden the clinical potential of MSC therapy.