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In this study, we aim to understand the impact of land use on the urban heat island (UHI) effect across an urban area. Considering the case study of Wuhan, China, land use factors and land surface temperatures (LSTs) of 589 planning management units were quantified in order to identify the spatial autocorrelation of LST, which indicated that a traditional regression would be invalid. By investigating the relationships between land use factors and the LST in summer, based on spatial regression models including the spatial lag model and the spatial error model, four conclusions were derived. First, the spatial error model effectively explains the relationships between LST and land use factors. Second, the impact on LST of the percentage of industrial areas is significant even though the impacts of land cover and building-group morphology indicators are combined, indicating that anthropogenic heat emission of industrial production contributes to high LSTs. Third, the relationship between the percentage of commercial area and LST is significant in the Pearson correlation analysis and traditional regression models, while not significant in spatial error model, suggesting that the urban heat environment of a commercial area is determined by the land use factors of the surrounding area. Fourth, the UHI effect in industrial and commercial areas could be precisely mitigated by not locating industrial areas beside residential areas, and setting up buffer zones between commercial areas and surrounding traditional residential areas. Overall, the results of this study innovatively deepen the understanding of the impact of the percentage of different urban land use types on the urban heat environment at the scale of planning management units, which is conducive to formulating precise regulation measures for mitigating UHI effects and improving public health.

Background Grafting is one of the promising techniques for improving abiotic stress tolerance in horticultural crops, but the underlying regulatory mechanisms of drought on grafted grapevine are largely unexplored. Results Herein, we investigated the phenotypic, physiologic, biochemical, and drought related genes change of self-rooted 1103P (1103 Paulsen), SM (Shine Muscat) and grafted SM/1103P (SM shoot/1103P root) under drought stress condition. The results indicated that grafted grapevine effectively alleviated drought damage in grape leaves by higher RWC, water potential and free water content. Drought stress led to the alterations of chlorophyll, carotenoid, photosynthetic parameters and chlorophyll fluorescence in grapevine leaves after drought treatment indicated grafted plants improved the photosystem response to drought stress. Moreover, grafted plants under drought stress exhibited higher levels of abscisic acid (ABA), indoleacetic acid (IAA) and soluble protein, but less contents of hydrogen peroxide (H 2 O 2 ) and malondialdehyde (MDA) both in leaves and roots. Drought stress also increased the activities of antioxidant enzymes (SOD, POD and CAT) and activated the transcript expression of VvCu/ZnSOD , VvPOD4 and VvCAT1 ) in both leaves and roots. Further expression analysis by real-time PCR indicated that the expression levels of ABA-dependent and ABA-independent related genes could be activated in grafted grape after drought treatment. Conclusions Taken together, our findings demonstrated that grafting onto 1103P enhanced tolerance against drought stress in grape by improving water content, photosynthesis and antioxidant defense capacity, which provided a valuable information for understanding the mechanisms of drought tolerance regulated by grafting plants.

Dysregulation of heat shock protein DNAJA2 induces genomic instability and was consequently hypothesized to promote tumorigenesis. However, DNAJA2 knockout mice do not develop cancer but exhibit neonatal lethality and the underlying mechanism remains unknown. Here, we demonstrate that DNAJA2 maintains homeostatic glucose metabolism by regulating insulin signaling. Mechanistically, DNAJA2 binds to the insulin receptor (IR) and prevents adaptor protein 2 (AP2)-mediated spontaneous IR endocytosis by inhibiting the IR-AP2 interaction. Thus, DNAJA2 defects lead to reduced IR localization on the plasma membrane and suppression of the insulin-stimulated signaling cascade, thereby inhibiting glycogen synthesis and storage in the liver during embryogenesis, further resulting in neonatal lethality of DNAJA2-deficient mice. Analysis of public datasets reveals a strong association between DNAJA2 and metabolic phenotypes, including type 2 diabetes mellitus (T2DM) and obesity, in both humans and mice. In conclusion, our study elucidates the mechanism by which DNAJA2 regulates IR endocytosis, insulin signaling and glucose metabolism, shedding light on the pathogenesis of metabolic disorders. Insulin signaling pathway is critical for glucose homeostasis maintenance. Here, the authors identify a role for DNAJA2 in regulating insulin signaling and glucose metabolism, loss of which causes insulin resistance and type 2 diabetes phenotypes.

The design and optimization of urban form has always been a hot topic in urban planning and development research. Besides, the creation of continuous vitality in urban areas is of critical importance in the development of urbanization. However, due to the lack of data, it is difficult to measure the effects of urban form on neighborhood vibrancy. Additionally, no uniform conclusion has been drawn regarding to what degree urban form can contribute to neighborhood vibrancy. Taking advantage of emerging new data sources, the depth and breadth of related research can now be improved. Therefore, this paper uses high-precision positioning social media check-in data to approximate the vibrancy of 658 neighborhoods, and uses a geographical information system (GIS) to quantitatively measure the urban form indicators in the central area of Chengdu City, China. A quantitative exploration and analysis of the relationships between neighborhood vibrancy and urban form is conducted. The results of three regression models considering different explanatory variables show that socio-economic factors account for approximately 23% of neighborhood vibrancy. In addition, the correlation between the shape characteristics of a neighborhood and the vibrancy is weak. However, when the inner urban form indicators of neighborhoods are introduced into the regression model, the goodness of fit (R2) is nearly doubled. This finding indicates that strong associations exist between urban form and neighborhood vibrancy. Specifically, building density and functional diversity are positively correlated with neighborhood vibrancy. Unlike existing studies, this study finds that the road network within the neighborhood plays a positive role in the creation of neighborhood vibrancy. However, the impact of a road density indicator is not as powerful as the impacts of building density and functional diversity. This research can help urban designers to better design urban environments.

Molecular chaperone HSP70s are attractive targets for cancer therapy, but their substrate broadness and functional non-specificity have limited their role in therapeutical success. Functioning as HSP70’s cochaperones, HSP40s determine the client specificity of HSP70s, and could be better targets for cancer therapy. Here we show that tumors defective in HSP40 member DNAJA2 are benefitted from immune-checkpoint blockade (ICB) therapy. Mechanistically, DNAJA2 maintains centrosome homeostasis by timely degrading key centriolar satellite proteins PCM1 and CEP290 via HSC70 chaperone-mediated autophagy (CMA). Tumor cells depleted of DNAJA2 or CMA factor LAMP2A exhibit elevated levels of centriolar satellite proteins, which causes aberrant mitosis characterized by abnormal spindles, chromosome missegregation and micronuclei formation. This activates the cGAS-STING pathway to enhance ICB therapy response in tumors derived from DNAJA2-deficient cells. Our study reveals a role for DNAJA2 to regulate mitotic division and chromosome stability and suggests DNAJA2 as a potential target to enhance cancer immunotherapy, thereby providing strategies to advance HSPs-based cancer therapy. Genomic instability can induce an anti-tumour immune response via activation of the cGAS-STING pathway following the formation of micronuclei (MN). Here, the authors identify a role for DNAJA2 in maintenance of chromosomal segregation. Loss of which increased MN formation and cGAS-STING pathway activation, increasing response to immune checkpoint blockade.

To investigate the diagnostic role of heart rate variability in breast cancer and its relationship with Carcinoembryonic antigen (CEA) in peripheral serum. We reviewed the electronic medical records of patients who attended Zhujiang Hospital of Southern Medical University between October 2016 and May 2019. The patients were grouped based on breast cancer history and were divided into two groups: breast cancer group(n = 19) and control group(n = 18). All women were invited for risk factor screening, including 24-hour ambulatory ECG monitoring and blood biochemistry after admission. The difference and correlation between the breast cancer group and control group were performed by comparing the heart rate variability and serum CEA levels. Additionally, diagnostic efficacy analysis of breast cancer was calculated by combining heart rate variability and serum CEA. In total, 37 patients were eligible for analysis, with 19 and 18 patients in the breast cancer group and control groups, respectively. Women with breast cancer had a significantly lower level of total LF, awake TP, and awake LF, and a significantly higher level of serum CEA compared with women with no breast cancer. Total LF, awake TP, and awake LF were negatively correlated with the CEA index (P < 0.05). The receiver operating characteristic (ROC) curves indicated the highest area under the curve (AUC) scores and specificity of the combination of awake TP, awake LF, and serum CEA (P < 0.05), while sensitivity was highest for total LF, awake TP, and awake LF (P < 0.05). Women with history of breast cancer had abnormalities in autonomic function. The combined analysis of heart rate variability and serum CEA analysis may have a predictive effect on the development of breast cancer and provide more basis for clinical diagnosis and treatment.

Biochar application is widely studied to mitigate the threats of soil degradation to food security and climate change. However, there are big variations in the effects of biochar application on crops, soils, and the atmosphere during crop production. This study provides a global dataset of biochar application effects on crop yield, soil properties, and greenhouse emissions. The dataset is extracted and integrated from 367 peer-reviewed studies with 891 independent field, laboratory, and incubation experiments across 37 countries. This dataset includes 21 variables before and after biochar application (including soil properties, crop yield, greenhouse gas emissions, etc.) of 2438 items, focusing on two main biochar application types: biochar application alone and combined with fertilizers. Background information on climate conditions, initial soil properties, management practices, and characteristics of biochar sources and production is also contained in the dataset. This dataset facilitates a comprehensive understanding of the impact of biochar application, supports the utilization of agricultural wastes for biochar production, and assists researchers in refining experimental protocols for further studies.

Mammalian male germ cell development is a stepwise cell-fate transition process; however, the full-term developmental profile of male germ cells remains undefined. Here, by interrogating the high-precision transcriptome atlas of 11,598 cells covering 28 critical time-points, we demonstrate that cell-fate transition from mitotic to post-mitotic primordial germ cells is accompanied by transcriptome-scale reconfiguration and a transitional cell state. Notch signaling pathway is essential for initiating mitotic arrest and the maintenance of male germ cells’ identities. Ablation of HELQ induces developmental arrest and abnormal transcriptome reprogramming of male germ cells, indicating the importance of cell cycle regulation for proper cell-fate transition. Finally, systematic human-mouse comparison reveals potential regulators whose deficiency contributed to human male infertility via mitotic arrest regulation. Collectively, our study provides an accurate and comprehensive transcriptome atlas of the male germline cycle and allows for an in-depth understanding of the cell-fate transition and determination underlying male germ cell development. The full-term developmental profile of male germ cells remains undefined. Here, the authors use single-cell sequencing to investigate the transcriptome landscapes of mouse male germ cells throughout development and find several critical regulators for prenatal cell-fate determination.

Since the founding of the country, China’s urban–rural relations have experienced four stages: differentiation, opposition, coordination, and equivalence. The scope of rural research has expanded from individual villages to group relations, and the research content also tends to the urban–rural integration trend of factor flow. In the context of New Urbanization and the Rural Revitalization Strategies, urban–rural integration is an important starting point to solve urban–rural contradictions, and the metropolitan area is an important spatial carrier to achieving urban–rural integration. Based on investigating the key issues of urban–rural integration and metropolitan governance, this paper constructs a “place function” cognitive framework of urban–rural space in the metropolitan area, and takes Wuhan metropolitan area with its dense rural distribution as an example to recognize the urban–rural spatial characteristics of the metropolitan area scale from six dimensions: spatial structure, land use, settlement distribution, scenic spots, agricultural enterprises, and service facilities. Then, it proposes a spatial development model of the Wuhan metropolitan area of the “point axis structure-, functional area-, resilient network”, and further explores the urban–rural integration path of “planning guidance, policy promotion, and spatial support”.

microRNAs play critical roles in the progression and metastasis of nonsmall cell lung cancer (NSCLC). miR-92b acts as an oncogene in some malignancies; however, its role in NSCLC remains poorly understood. Here, we found that miR-92b was significantly increased in human NSCLC tissues and cell lines. Inhibition of miR-92b remarkably suppressed cell proliferation, migration, and invasion of NSCLC cells. Reversion-inducing-cysteine-rich protein with kazal motifs (RECK) was identified to be a target of miR-92b. Expression of miR-92b was negatively correlated with RECK in NSCLC tissues. Collectively, miR-92b might promote NSCLC cell growth and motility partially by inhibiting RECK.