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The experiment was carried out in the plant canopy of the Department of Horticulture and Landscape Engineering/College of Agriculture, University of Kerbala, located in the Al-Husseiniyah area in Kerbala Governorate, on 11/10/2023 to 1/7/2024. The experiment aimed to determine the best level of palm frond waste, along with determining the best concentration of tryptophan acid and the extent of their effect. On some traits of vegetative growth of olive seedlings, the experiment was carried out according to a randomized complete block design (RCBD) according to the order of the factorial experiment, with three replications. The first factor included two varieties of olive (Manzanillo and Bashiqi), while the second factor included three levels of palm frond waste (0, 3% and 6%) and the third factor included three concentrations of tryptophan acid (0, 75 and 150 ml L −1 ). The results showed that the Manzanillo variety was superior in the content of chlorophyll, carbohydrates, nitrogen, phosphorus and potassium, as well as in the protein percentage, with averages of (052.3ml g −1 , 45704%,25692%, 35060%, 1.723%, and 56582.% respectively). The results also showed that palm frond waste had a significant effect, as the level (6%) was superior in the above-mentioned traits with averages of(3.810mg.g −1 , 6.621%, 3.254%, 0.437%, 1.823% and 20.342% respectively). As for the concentrations of spraying with tryptophan acid, it was noted that the concentration (175 ml L −1 ) was superior for the same traits, as it gave averages of (3.310ml g −1 , 45995%,2.648%, 0.357%, 1.739% and16.554% respectively). It was also noted that the interactions between the factors were significant in all the traits under study.