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The co-occurrence of drought and heat significantly hampers plant productivity. Although their impacts are well studied, these studies have been based on the effects of individual stressors rather than their combined influence. Okra is crucial for food and nutritional security and livelihoods in many regions, yet it remains under-researched and unimproved. Okra has been proven to be sensitive to both drought and heat stress. This study employed a cost-effective phenotyping method to assess key traits characterising the diversity of okra morphophysiological responses to independent and interactive heat-drought stresses. This study aimed to understand okra responses to stress, identify stress-resilient traits, and characterise okra genotypes. We also addressed the need to examine interactive stress effects, which mirror real-world scenarios more accurately than single-stress studies. Sixty-three okra genotypes were subjected to heat, drought, or concurrent heat-drought stress at the seedling stage in improvised climate-controlled chambers. The germplasm exhibited significant variations in response to the various stresses. The broad-sense heritability was high (> 0.60) for traits such as chlorophyll content, plant biomass, performance indices, electrolyte leakage, and total leaf area. Drought stress alone had a more pronounced effect than heat stress alone, and the adverse impact was worsened under combined heat and drought stress. The interactive impact of drought and heat was more likely additive than antagonistic or synergistic. A positive and strong relationship was observed between photosynthetic efficiency parameters such as the Fv/Fm ratio, chlorophyll content, relative water index, and biomass parameters such as dry shoot weight. The 63 okra genotypes were classified into three distinct clusters, suggesting potential for future breeding efforts. Okra genotype considered to be tolerant or climate resilient (such as GH170, V1060831, GH174, V1060874, and GH106) to drought and heat, maintained enhanced photosynthetic efficiency and high internal water potential, possibly reducing osmotic and oxidative damage. This study revealed some mechanisms underlying the adaptation of okra genotypes to independent and combined heat and drought stress. The results provide a basis for breeding efforts to develop climate-resilient okra varieties.

Drought and heat are two stresses that often occur together and may pose significant risks to crops in future climates. However, the combined effects of these two stressors have received less attention than single-stressor investigations. This study used a rapid and straightforward phenotyping method to quantify the variation in 128 African eggplant genotype responses to drought, heat, and the combined effects of heat and drought at the seedling stage. The study found that the morphophysiological traits varied significantly among the 128 eggplants, highlighting variation in response to abiotic stresses. Broad-sense heritability was high (> 0.60) for chlorophyll content, plant biomass and performance index, electrolyte leakage, and total leaf area. Positive and significant relationships existed between biomass and photosynthetic parameters, but a negative association existed between electrolyte leakage and morpho-physiological traits. The plants underwent more significant stress when drought and heat stress were imposed concurrently than under single stresses, with the impact of drought on the plants being more detrimental than heat. There were antagonistic effects on the morphophysiology of the eggplants when heat and drought stress were applied together. Resilient genotypes such as RV100503, RV100501, JAMBA, LOC3, RV100164, RV100169, LOC 3, RV100483, GH5155, RV100430, GH1087, GH1087*, RV100388, RV100387, RV100391 maintained high relative water content, low electrolyte leakage, high Fv/Fm ratio and performance index, and increased biomass production under abiotic stress conditions. The antagonistic interactions between heat and drought observed here may be retained or enhanced during several stress combinations typical of plants’ environments and must be factored into efforts to develop climate change-resilient crops. This paper demonstrates improvised climate chambers for high throughput, reliable, rapid, and cost-effective screening for heat and drought and combined stress tolerance in plants.

Drought and heat are two stresses that often occur together and may pose significant risks to crops in future climates. However, the combined effects of these two stressors have received less attention than single-stressor investigations. This study used a rapid and straightforward phenotyping method to quantify the variation in 128 African eggplant genotype responses to drought, heat, and the combined effects of heat and drought at the seedling stage. The study found that the morphophysiological traits varied significantly among the 128 eggplants, highlighting variation in response to abiotic stresses. Broad-sense heritability was high (> 0.60) for chlorophyll content, plant biomass and performance index, electrolyte leakage, and total leaf area. Positive and significant relationships existed between biomass and photosynthetic parameters, but a negative association existed between electrolyte leakage and morpho-physiological traits. The plants underwent more significant stress when drought and heat stress were imposed concurrently than under single stresses, with the impact of drought on the plants being more detrimental than heat. There were antagonistic effects on the morphophysiology of the eggplants when heat and drought stress were applied together. Resilient genotypes such as RV100503, RV100501, JAMBA, LOC3, RV100164, RV100169, LOC 3, RV100483, GH5155, RV100430, GH1087, GH1087*, RV100388, RV100387, RV100391 maintained high relative water content, low electrolyte leakage, high Fv/Fm ratio and performance index, and increased biomass production under abiotic stress conditions. The antagonistic interactions between heat and drought observed here may be retained or enhanced during several stress combinations typical of plants' environments and must be factored into efforts to develop climate change-resilient crops. This paper demonstrates improvised climate chambers for high throughput, reliable, rapid, and cost-effective screening for heat and drought and combined stress tolerance in plants.