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Background: The aim of this case series report is to evaluate the characteristics of multisystem inflammatory syndrome (MIS) in neonates following prenatal maternal COVID-19 infection. Methods: We present a case series of three newborns (≤28 days of age) diagnosed with MIS due to the vertical transmission of SARS-CoV2 infection and admitted from 1 January 2021 to 1 June 2023. The inclusion criteria were negative RT-PCR-SARS-CoV-2 test in infants, initial negative IgM-SARS-CoV-2 in infants followed by the emergence of positive IgG-SARS-CoV-2 antibodies in infants and maternal COVID-19 infection in the third trimester of pregnancy. Patients enrolled in this case series were admitted due to acute febrile illnesses. Results: All three cases occurred in patients born at a mean gestational age of 39 weeks and who were appropriate for gestational age. The mean age at admission was 18.3 days. Fibrinogen (>400 mg/dL) and ferritin (>120 mg/dL) were elevated above the upper normal limit. Elevated levels of myocardial biomarkers (D-dimers, N-terminal pro b-type natriuretic peptide troponin T and creatine phosphokinase myocardial band) were recorded, with normal heart function evaluated using echocardiography. All three patients were treated with antibiotics; one received intravenous immunoglobulin. A 4-week follow-up was completed in two patients when their myocardial biomarkers and ferritin were still elevated but lower compared with previous examinations. D-dimers levels were normalized in 2/3 patients. Conclusions: Subclinical myocarditis was diagnosed as an early outcome in infants with MIS diagnosed postnatally due to the vertical transmission of SARS-CoV2 infection and may represent a new challenge for pediatricians in the pandemic era.

Introduction: During the COVID pandemic, there was a need for rapid and accessible detection of SARS-CoV-2 viral infections to assess the exact number of infected people worldwide to take appropriate medical measures. Objective/Aims: The paper proposes an analysis of expert information regarding the particularities of the SARS-CoV-2 virus and the possibilities of detecting anti-SARS-CoV-2 antibodies and viral antigens. Materials and Methods: Studies from the specialized literature that presented information about the working procedures and configurations of anti-SARS-CoV-2 antibody and viral antigen detection systems from the pandemic period and later were considered. Results: RT-PCR testing, the current gold standard for the diagnosis of COVID-19, permits the detection of viral genetic material, a sensitive technique that allows rapid detection in the early phase of the virus. It has been identified that serological tests are recommended for the diagnosis of COVID-19 patients during the acute and early subacute phases. Rapid tests have been developed for the detection of viral antigens or human anti-SARS-CoV-2 antibodies in various biological samples, with a view to frequent monitoring of people working in environments with a high risk of infection. Conclusion: In recent years, significant progress has been made in the field of cell biology, combining high-performance studies carried out in research laboratories with testing and validation of methods quickly implemented in the specialized laboratories of infectious diseases hospitals and not only but also with the development of rapid tests with the possibility of direct purchase by the population. Rezumat Introducere: In timpul pandemiei COVID s-a impus necesitatea detectării rapide și accesibile a infecțiilor virale cu SARS-CoV-2 pentru a evalua numărul exact de persoane infectate în toată lumea cu scopul de a lua măsurile medicale potrivite. Obiectivul principal al studiului: Lucrarea își propune o analiză a informațiilor de specialitate privind particularități ale virusului SARS-CoV-2 și a posibilităților de detectare a anticorpilor anti-SARS-CoV-2 și a antigenelor virale. Material și metodă: S-au considerat studii din literatura de specialitate care au prezentat informații despre metodele de lucru și configurațiile sistemelor de detecție a anticorpilor anti-SARS-CoV-2 și a antigenelor virale din perioada pandemiei și ulterior. Rezultate: Testarea RT-PCR, standardul de aur actual pentru diagnosticarea COVID-19, favorizează detectarea materialului genetic viral, tehnică sensibilă și care permite detectarea rapidă și în fază incipientă a virusului. S-au identificat că teste serologice sunt recomandate pentru diagnosticarea pacienților COVID-19 în timpul fazei acute și subacute precoce. S-au dezvoltat teste rapide pentru detectarea antigenelor virale sau a anticorpilor umani anti-SARS-CoV-2 din diferite probe biologice, în vederea monitorizării frecvente a persoanelor care își desfășurau activitatea în mediile cu risc crescut de infecție. Concluzii: In ultimii ani s-au realizat progrese semnificative în domeniul biologiei celulare, combinând studii performante realizate in laboratoarele de cercetare cu testări și validări de metode implementate rapid în laboratoarele de specialitate ale spitalelor de boli infecțioase și nu numai, dar și cu elaborarea de teste rapide cu posibilitatea de achiziționare directă de către populație.

Materials and Methods: Studies from the specialized literature that presented information about the working procedures and configurations of anti-SARS-CoV-2 antibody and viral antigen detection systems from the pandemic period and later were considered. Results: RT-PCR testing, the current gold standard for the diagnosis of COVID-19, permits the detection of viral genetic material, a sensitive technique that allows rapid detection in the early phase of the virus. Conclusion: In recent years, significant progress has been made in the field of cell biology, combining high-performance studies carried out in research laboratories with testing and validation of methods quickly implemented in the specialized laboratories of infectious diseases hospitals and not only but also with the development of rapid tests with the possibility of direct purchase by the population. Dintre cele patru gene structurale, SARS-CoV-2 împarte mai mult de 90% de aminoacizi cu SARS-CoV, cu excepția genei S

Congenital ichthyosis, a continuous challenge for the medical practitioner, represents a skin keratinization disorder and also a persistence of skin desquamation. There are many clinical forms, but the most common is ichthyosis Vulgaris. We would like to present two clinical cases that were admitted to Children's Clinic Hospital in 2020 and that had different clinical evolutions. Both infants had complications regarding skin infection, enterocolitis, malnutrition, and dehydration. Nevertheless, it is known that congenital ichthyosis represents a significant cause of morbidity and mortality among children and that it has a major impact on the quality of life, both of the patients and their family members. Rezumat Ihtioza congenitală, o provocare de actualitate pentru medicul clinician, reprezintă un grup heterogen de boli, caracterizat prin descuamare tegumentară persistentă și prezenta hiperkeratozei, existând sub mai multe forme, cea mai comună fiind ihtioza vulgaris (forma autozomal semi-dominantă). În actualul articol dorim să prezentăm două cazuri clinice, care s-au internat în cadrul Spitalului Clinic de Urgență pentru Copii Brașov, în anul 2020. Sugarii internați au avut evoluții diferite, confruntându-se cu complicații legate de funcția de barieră a pielii afectate, cum ar fi deshidratarea și infecția tegumentară. Mai mult, ambii sugari au avut un grad de malnutriție dobândită și enterocolită cu Rotavirus. Reținem că ihtioza reprezintă o cauză de morbiditate și mortalitate semnificativă, având un impact semnificativ atât asupra calității vieții pacienților, cât și asupra familiilor acestora.

It is known that cigarette smoking is correlated with medical associated inquires. New electronic cigarettes are intensively advertised as an alternative to conventional smoking, but only a few studies demonstrate their harmful potential. A cross-sectional study was designed using 150 subjects from Brasov (Romania), divided into three groups: non-smokers (NS = 58), conventional cigarettes smokers (CS = 58) and electronic cigarettes users (ECS = 34). The aim of this study was to determine levels of some plasma lipophilic and hematological components, and the total antioxidant status that could be associated with the smoking status of the subjects. Serum low density lipoproteins (LDL) cholesterol increased significantly for ECS participants versus NS group (18.9% difference) (p < 0.05). Also, the CS group is characterized by an increase of serum LDL cholesterol (7.9% difference vs. NS), but with no significant statistical difference. The variation of median values of serum very low density lipoproteins (VLDL) was in order NS < ECS < CS, with statistical difference between NS and CS groups (34.6% difference; p = 0.023). When comparing the antioxidant status of the three groups, significant differences (p < 0.05) were obtained between NS vs. CS and NS vs. ECS. Similar behavior was identified for CS and ECS. Statistically significant changes (p < 0.0001) for both vitamin A and vitamin E were identified in the blood of NS vs. CS and NS vs. ECS, and also when comparing vitamin A in the blood of the CS group versus the ECS group (p < 0.05). When all groups were compared, the difference in the white blood cell (WBC) was (p = 0.008). A slight increase in the red blood cell (RBC) count was observed, but with no statistical difference between groups. These results indicated that conventional cigarette and e-cigarette usage promotes the production of excess reactive oxygen species, involving different pathways, different antioxidants and bioactive molecules.

[...]it is known that congenital ichtyosis represents a significant cause of morbidity and mortality among children and that it has a major impact among the quality of life, both of the patients and their family members. (IP=0,80), facies dismorfic, tegumente eritrodermice cu descuamări în lambouri (Fig.nr.1), alopecie, ţesut adipos slab reprezentat, clonii ale membrelor, echilibrat cardiorespirator, cu abdomen suplu depresibil, tranzit intestinal şi diureză prezentă, fără semne de iritaţie meningeană. Nr.2-3) Analizele de laborator (Tabel nr.1-2) relevă hemoleucogramă cu uşoară leucocitoză o hipoproteinemie marcată 3,5g/dl, sindrom biologic inflamator înalt cu hemocultură pozitivă pentru Staphylococcus aureus, cultură din secreţie otică pozitivă pentru E.Coli şi Pseudomonas aeruginosa. Caz clinic 2 C.S. sugar în vârstă de 3 luni şi 2 săptămâni se prezintă la camera de gardă pentru scaune diareice, tuse, rinoree şi inapetenţă cu debut de o zi, este în tratament cu Fenobarbital.

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The quality of marketed pharmaceutical formulations must be guaranteed to attain better remedial effects and lower toxicity. The wide exploitation of antibiotics may lead to their presence as residues in body fluids and wastewaters, potentially toxic to human health. Consequently, determining antibiotics in pharmaceutical formulations and water samples is of significant importance. This paper aims to explore the possibilities of a high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) method to obtain a simple, fast, and efficient analytical tool for the simultaneous determination of antibiotics in pharmaceutical formulations and environmental samples. The method was completely validated with regard to specificity, linearity, detection and quantification limits, precision, accuracy, and robustness according to the requirements of existing guidelines, and was proven to be reliable and suitable for the envisioned application. The linearity study was conducted for the calibration curves in the range of 10–100 μg/mL. The limits of detection and quantification were found to be 0.2 and 0.7 μg/mL for amoxicillin and 0.3 and 1.0 μg/mL for doxycycline, respectively. The high recovery of drugs from their commercial pharmaceutical formulations (93%) and from wastewater samples (98%) indicated good accuracy and precision. The method is robust for small or deliberate changes to the chromatographic parameters, and it was successfully applied for the quantitative determination of amoxicillin and doxycycline in wastewater and commercial tablets. The obtained results proved that the validated method is appropriate for its intended use in the routine quality control and assay of both antibiotics studied.

Background: Sinus-lift (SL) is a pre-prosthetic procedure with the objective of increasing bone height to achieve implant insertion primary stability in implant-supported prostheses. The biomechanical properties of SL augmentation materials are influenced by their origin, manufacture, bioactive substances addition, receiver, and surgical procedure. This systematic review provides insights into state-of-the-art SL biomaterials, focusing on autologous bone grafting as the gold standard. Methods: The study followed the PRISMA flow diagram, searching WoS (Web of Science), Embase, Cochrane, and PubMed databases using the search terms «sinus lift» OR «sinus augmentation» OR «bone graft» OR «bovine» OR «porcine» OR «autologous» OR «allogenic» OR «xenogeneic» OR «alloplastic» OR «hydroxyapatite» OR «β-tricalcium phosphate (β-TCP)» OR «equine» OR «PRF». Results: The highest bone gain was provided by Bioglass at 42%. Articles written between 2014 and 2024 in English or French, containing human studies and with full text available, were included. Participants were required to be in good general health, without acute, chronic, or congenital diseases, or substance abuse (drugs, alcohol, or nicotine). SL surgery was performed using the lateral approach, with no Schneiderian membrane perforation or postoperative complications. The network meta-analysis was conducted using the R statistical computing environment. To assess the inconsistency between direct and indirect evidence, we used a net heat plot. To evaluate heterogeneity across studies, we used the chi-squared-based Q-test and I2 statistic. A significance level of 0.05 was applied throughout all analyses. Results: Allogeneic bovine bone and hydrox yapatite demonstrated the lowest resorption rates. Significant differences were found for residual graft and connective tissue between allogenous bovine bone (ABB) + AlB vs. β-TCP + PRF (p = 0.028); ABB + AlB vs. β-TCP (p = 0.034); ABB + AlB vs. BCP (p = 0.037). Meta-analysis showed that the overall heterogeneity was 51.8% (6.9–75%; p = 0.019), with significant heterogeneity within designs (p = 0.007) and no significant heterogeneity between designs (p = 0.39). AB had a better bone regeneration ratio compared to many of the other interventions, but only two passed the threshold of significance: A1B and B-TCP + AB. Conclusions: A grafting material’s superiority is determined by its new bone formation ratio, connective tissue integration, residual graft content, and bone resorptionratio. Although autologous bone grafting has exhibited superior bone regeneration compared to other biomaterials, it was not favored due to its unpredictable connective tissue concentration and bone resorption ratio. Additionally, autologous bone exhibited the fastest metabolic turnover among all grafting materials.