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Quantifying soil organic carbon stocks (SOC) and their dynamics accurately is crucial for better predictions of climate change feedbacks within the atmosphere-vegetation-soil system. However, the components, environmental responses and controls of the soil CO2 efflux (Rs ) are still unclear and limited by field data availability. The objectives of this study were (1) to quantify the contribution of the various Rs components, specifically its mycorrhizal component, (2) to determine their temporal variability, and (3) to establish their environmental responses and dependence on gross primary productivity (GPP). In a temperate deciduous oak forest in south east England hourly soil and ecosystem CO2 fluxes over four years were measured using automated soil chambers and eddy covariance techniques. Mesh-bag and steel collar soil chamber treatments prevented root or both root and mycorrhizal hyphal in-growth, respectively, to allow separation of heterotrophic (Rh ) and autotrophic (Ra ) soil CO2 fluxes and the Ra components, roots (Rr ) and mycorrhizal hyphae (Rm ). Annual cumulative Rs values were very similar between years (740 ± 43 g C m-2 yr-1 ) with an average flux of 2.0 ± 0.3 μmol CO2 m-2 s-1 , but Rs components varied. On average, annual Rr , Rm and Rh fluxes contributed 38, 18 and 44%, respectively, showing a large Ra contribution (56%) with a considerable Rm component varying seasonally. Soil temperature largely explained the daily variation of Rs (R2 = 0.81), mostly because of strong responses by Rh (R2 = 0.65) and less so for Rr (R2 = 0.41) and Rm (R2 = 0.18). Time series analysis revealed strong daily periodicities for Rs and Rr , whilst Rm was dominated by seasonal (~150 days), and Rh by annual periodicities. Wavelet coherence analysis revealed that Rr and Rm were related to short-term (daily) GPP changes, but for Rm there was a strong relationship with GPP over much longer (weekly to monthly) periods and notably during periods of low Rr . The need to include individual Rs components in C flux models is discussed, in particular, the need to represent the linkage between GPP and Ra components, in addition to temperature responses for each component. The potential consequences of these findings for understanding the limitations for long-term forest C sequestration are highlighted, as GPP via root-derived C including Rm seems to function as a C \"overflow tap\", with implications on the turnover of SOC.

$\\bullet$ Although arbuscular mycorrhizal (AM) fungi are a major pathway in the global carbon cycle, their basic biology and, in particular, their respiratory response to temperature remain obscure. $\\bullet$ A pulse label of the stable isotope 13C was applied to Plantago lanceolata, either uninoculated or inoculated with the AM fungus Glomus mosseae. The extra-radical mycelium (ERM) of the fungus was allowed to grow into a separate hyphal compartment excluding roots. We determined the carbon costs of the ERM and tested for a direct temperature effect on its respiration by measuring total carbon and the $^{13}C:^{12}C$ ratio of respired CO2. With a second pulse we tested for acclimation of ERM respiration after 2 wk of soil warming. $\\bullet$ Root colonization remained unchanged between the two pulses but warming the hyphal compartment increased ERM length. δ13C signals peaked within the first 10 h and were higher in mycorrhizal treatments. The concentration of CO2 in the gas samples fluctuated diurnally and was highest in the mycorrhizal treatments but was unaffected by temperature. Heating increased ERM respiration only after the first pulse and reduced specific ERM respiration rates after the second pulse; however, both pulses strongly depended on radiation flux. $\\bullet$ The results indicate a fast ERM acclimation to temperature, and that light is the key factor controlling carbon allocation to the fungus.

Ecosystem responses to global warming will be complex and varied. Ecosystem warming experiments hold great potential for providing insights on ways terrestrial ecosystems will respond to upcoming decades of climate change. Documentation of initial conditions provides the context for understanding and predicting ecosystem responses.

The results of published and unpublished experiments investigating the impacts of elevated [CO₂] on the chemistry of leaf litter and decomposition of plant tissues are summarized. The data do not support the hypothesis that changes in leaf litter chemistry often associated with growing plants under elevated [CO₂] have an impact on decomposition processes. A meta-analysis of data from naturally senesced leaves in field experiments showed that the nitrogen (N) concentration in leaf litter was 7.1% lower in elevated [CO₂] compared to that in ambient [CO₂]. This statistically significant difference was: (1) usually not significant in individual experiments, (2) much less than that often observed in green leaves, and (3) less in leaves with an N concentration indicative of complete N resorption. Under ideal conditions, the efficiency with which N is resorbed during leaf senescence was found not to be altered by CO₂ enrichment, but other environmental influences on resorption inevitably increase the variability in litter N concentration. Nevertheless, the small but consistent decline in leaf litter N concentration in many experiments, coupled with a 6.5% increase in lignin concentration, would be predicted to result in a slower decomposition rate in CO₂-enriched litter. However, across the assembled data base, neither mass loss nor respiration rates from litter produced in elevated [CO₂] showed any consistent pattern or differences from litter grown in ambient [CO₂]. The effects of [CO₂] on litter chemistry or decomposition were usually smallest under experimental conditions similar to natural field conditions, including open-field exposure, plants free-rooted in the ground, and complete senescence. It is concluded that any changes in decomposition rates resulting from exposure of plants to elevated [CO₂] are small when compared to other potential impacts of elevated [CO₂] on carbon and N cycling. Reasons for experimental differences are considered, and recommendations for the design and execution of decomposition experiments using materials from CO₂-enrichment experiments are outlined.

Fluxes of nitrous oxide, methane and carbon dioxide were measured from soils under ambient (350 µL L^sup -1^) and enhanced (600 µL L^sup -1^) carbon dioxide partial pressures (pCO^sub 2^) at the 'Free Air Carbon Dioxide Enrichment' (FACE) experiment, Eidgenössische Technische Hochschule (ETH), Eschikon, Switzerland in July 1995, using a GC housed in a mobile laboratory. Measurements were made in plots of Lolium perenne maintained under high N input. During the data collection period N fertiliser was applied at a rate of 14 g m^sup -2^ of N. Elevated pCO^sub 2^ appeared to result in an increased (27%) output of N^sub 2^O, thought to be the consequence of enhanced root-derived available soil C, acting as an energy source for denitrification. The climate, agricultural practices and soils at the FACE experiment combined to give rise to some of the largest N^sub 2^O emissions recorded for any terrestrial ecosystem. The amount of CO^sub 2^-C being lost from the control plot was higher (10%) than for the enhanced CO^sub 2^ plot, and is the reverse of that predicted. The control plot oxidised consistently more CH^sub 4^ than the enhanced plot, oxidising 25.5 ± 0.8 µg m^sup -2^ hr^sup -1^ of CH^sub 4^ for the control plot, with an average of 8.5 ± 0.4 µg m^sup -2^ hr^sup -1^ of CH^sub 4^ for the enhanced CO^sub 2^ plot. This suggests that elevated pCO^sub 2^ may lead to a feedback whereby less CH^sub 4^ is removed from the atmosphere. Despite the limited nature of the current study (in time and space), the observations made here on the interactions of elevated pCO^sub 2^ and soil trace gas release suggest that significant interactions are occurring. The feedbacks involved could have importance at the global scale.[PUBLICATION ABSTRACT]

Terrestrial ecosystems contain large amounts of carbon (C) and have the potential to significantly increase atmospheric carbon dioxide (CO2) concentrations. Peatlands are particularly important for C storage, although little is known about the effects of anthropogenic activities on C balance in these ecosystems. Sheep-grazing and rotational burning are widely practised on blanket peat moorlands in the United Kingdom. The effects of these activities on C sequestration in peat has been investigated with a long-term randomized block experiment with treatments: (a) grazed + unburnt; (b) grazed + burnt every ten years; (c) ungrazed + unburnt. C accumulation under these treatments was compared by identifying a chronologically synchronous horizon within the peat common to all treatment plots. This fixed point was defined by the ‘take-off’ in concentration of spheroidal carbonaceous particles and was supported by the record of charcoal fragments. There was no significant difference in recent C accumulation rates between lightly grazed and ungrazed plots. In contrast, after 30 years there was significantly less C stored in the blanket peat in plots which had been burned every ten years. The results indicate that light sheep-grazing at this site did not affect rates of C accumulation in blanket peat, but decadal burning of moorland reduced C sequestration.

Vegetation composition was found to be an important factor controlling CH₄ emission from an ombrotrophic peatland in the UK, with significantly greater (P < 0.01) CH₄ released from areas containing both Eriophorum vaginatum L. and Sphagnum, than from similar areas without E. vaginatum. Positive correlations were observed between the amount of E. vaginatum and CH₄ emission, with the best predictor of flux being the amount of below-ground biomass of this species (r² = 0.93). A cutting experiment revealed that there was no significant difference (P > 0.05) in CH₄ flux between plots with E. vaginatum stems cut above the water table and plots with intact vegetation, yet there was a 56% mean reduction in CH₄ efflux where stems were cut below the water table (P < 0.05). The effect of E. vaginatum on CH₄ release was mimicked by the presence of inert glass tubes. These findings suggest that the main short-term role of E. vaginatum in the ecosystem is simply as a conduit for CH₄ release. The longer-term importance of E. vaginatum in controlling CH₄ fluxes through C substrate input was suggested by the positive correlation between the night-time CO₂ and CH₄ fluxes (r² = 0.70), which only occurred when the vegetation was not senescent.

Ash (Fraxinus excelsior L.), birch (Betula pubescens Ehrh.), sycamore (Acerpseudoplatanus L.) and Sitka spruce (Picea sitchensis (Bong.) Carr.) leaf litters were monitored for decomposition rates and nutrient release in a laboratory microcosm experiment. Litters were derived from solar domes where plants had been exposed to two different CO₂ regimes: ambient (350 μL L⁻¹ CO₂) and enriched (600 μL L⁻¹ CO₂). Elevated CO₂ significantly affected some of the major litter quality parameters, with lower N, higher lignin concentrations and higher ratios of C/N and lignin/N for litters derived from enriched CO₂. Respiration rates of the deciduous species were significantly decreased for litters grown under elevated CO₂, and reductions in mass loss at the end of the experiment were generally observed in litters derived from the 600 ppm CO₂ treatment. Nutrient mineralization, dissolved organic carbon, and pH in microcosm leachates did not differ significantly between the two CO₂ treatments for any of the species studied. Litter quality parameters were examined for correlations with cumulative respiration and decomposition rates: N concentration, C/N and lignin/N ratios showed the highest correlations, with differences between litter types. The results indicate that higher C storage will occur in soil as a consequence of litter quality changes resulting from higher atmospheric concentrations of CO₂.

Plant roots harbor a large diversity of microorganisms that have an essential role in ecosystem functioning. To better understand the level of intimacy of root-inhabiting microbes such as arbuscular mycorrhizal fungi and bacteria, we provided ¹³CO₂ to plants at atmospheric concentration during a 5-h pulse. We expected microbes dependent on a carbon flux from their host plant to become rapidly labeled. We showed that a wide variety of microbes occurred in roots, mostly previously unknown. Strikingly, the greatest part of this unsuspected diversity corresponded to active primary consumers. We found 17 bacterial phylotypes co-occurring within roots of a single plant, including five potentially new phylotypes. Fourteen phylotypes were heavily labeled with the ¹³C. Eight were phylogenetically close to Burkholderiales, which encompass known symbionts; the others were potentially new bacterial root symbionts. By analyzing unlabeled and ¹³C-enriched RNAs, we demonstrated differential activity in C consumption among these root-inhabiting microbes. Arbuscular mycorrhizal fungal RNAs were heavily labeled, confirming the high carbon flux from the plant to the fungal compartment, but some of the fungi present appeared to be much more active than others. The results presented here reveal the possibility of uncharacterized root symbioses.

For convenience, measurements used to compare soil respiration (Rs) from different land uses, crops or management practices are often made between 09:00 and 16:00 UTC, convenience which is justified by an implicit assumption that Rs is largely controlled by temperature. Three months of continuous data presented here show distinctly different diurnal patterns of Rs between barley (Hordeum vulgare) and Miscanthus  ×  giganteus (Miscanthus) grown on adjacent fields. Maximum Rs in barley occurred during the afternoon and correlated with soil temperature, whereas in Miscanthus after an initial early evening decline, Rs increased above the daily average during the night and in July maximum daily rates of Rs were seen at 22:00 and was significantly correlated with earlier levels of solar radiation, probably due to delays in translocation of recent photosynthate. Since the time of the daily mean Rs in Miscanthus occurred when Rs in the barley was 40 % greater than the daily mean, it is vital to select appropriate times to measure Rs especially if only single daily measurements are to be made.