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"James, Lincoln"
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A prickle of porcupines
This book describes the physical characteristis, behavior, habitat, and group life of porcupines.
Book
Insights into the Activity and Substrate Binding of Xylella fastidiosa Polygalacturonase by Modification of a Unique QMK Amino Acid Motif Using Protein Chimeras
Polygalacturonases (EC 3.2.1.15) catalyze the random hydrolysis of 1, 4-alpha-D-galactosiduronic linkages in pectate and other galacturonans. Xylella fastidiosa possesses a single polygalacturonase gene, pglA (PD1485), and X. fastidiosa mutants deficient in the production of polygalacturonase are non-pathogenic and show a compromised ability to systemically infect grapevines. These results suggested that grapevines expressing sufficient amounts of an inhibitor of X. fastidiosa polygalacturonase might be protected from disease. Previous work in our laboratory and others have tried without success to produce soluble active X. fastidiosa polygalacturonase for use in inhibition assays. In this study, we created two enzymatically active X. fastidiosa / A. vitis polygalacturonase chimeras, AX1A and AX2A to explore the functionality of X. fastidiosa polygalacturonase in vitro. The AX1A chimera was constructed to specifically test if recombinant chimeric protein, produced in Escherichia coli, is soluble and if the X. fastidiosa polygalacturonase catalytic amino acids are able to hydrolyze polygalacturonic acid. The AX2A chimera was constructed to evaluate the ability of a unique QMK motif of X. fastidiosa polygalacturonase, most polygalacturonases have a R(I/L)K motif, to bind to and allow the hydrolysis of polygalacturonic acid. Furthermore, the AX2A chimera was also used to explore what effect modification of the QMK motif of X. fastidiosa polygalacturonase to a conserved RIK motif has on enzymatic activity. These experiments showed that both the AX1A and AX2A polygalacturonase chimeras were soluble and able to hydrolyze the polygalacturonic acid substrate. Additionally, the modification of the QMK motif to the conserved RIK motif eliminated hydrolytic activity, suggesting that the QMK motif is important for the activity of X. fastidiosa polygalacturonase. This result suggests X. fastidiosa polygalacturonase may preferentially hydrolyze a different pectic substrate or, alternatively, it has a different mechanism of substrate binding than other polygalacturonases characterized to date.
Journal Article
Advancing Industrial Process Electrification and Heat Pump Integration with New Exergy Pinch Analysis Targeting Techniques
The process integration and electrification concept has significant potential to support the industrial transition to low- and net-zero-carbon process heating. This increasingly essential concept requires an expanded set of process analysis tools to fully comprehend the interplay of heat recovery and process electrification (e.g., heat pumping). In this paper, new Exergy Pinch Analysis tools and methods are proposed that can set lower bound work targets by acutely balancing process heat recovery and heat pumping. As part of the analysis, net energy and exergy load curves enable visualization of energy and exergy surpluses and deficits. As extensions to the grand composite curve in conventional Pinch Analysis, these curves enable examination of different pocket-cutting strategies, revealing their distinct impacts on heat, exergy, and work targets. Demonstrated via case studies on a spray dryer and an evaporator, the exergy analysis targets net shaft-work correctly. In the evaporator case study, the analysis points to the heat recovery pockets playing an essential role in reducing the work target by 25.7%. The findings offer substantial potential for improved industrial energy management, providing a robust framework for engineers to enhance industrial process and energy sustainability.
Journal Article
2021 STARS conference: Student plenary
The Student Panel Session at the 2021 STARS Conference concluded the formal presentations for the event. At this session, students from the tertiary sector shared with delegates their personal experiences in higher education and thoughts concerning the messages and insights gained from the conference experience. The students had responded to an invitation from Student Voice Australia to participate in the conference, be part of the Panel, and were encouraged to attend the keynote presentations across the event. Delegates had the opportunity to present questions to the students. For the purposes of this feature, the editors have summarised and edited the transcript to present the key points of each discussion, including questions and comments from delegates. Panel members have approved the editorial interpretations of their comments.
Journal Article
Keiretsu, Governance, and Learning: Case Studies in Change from the Japanese Automotive Industry
The keiretsu structuring of assembler-supplier relations historically enabled Japanese auto assemblers to remain lean and flexible while enjoying a level of control over supply akin to that of vertical integration. Yet currently there is much talk of breakdowns in keiretsu networks. This paper examines some recent developments in Japanese parts-supply keiretsu .
We argue that keiretsu relationships are drifting from \"hybrid\" or \"network\" (i.e., keiretsu ) governance modes toward the extremes of arms-length contracting and top-down administration. These changes are best understood through a combination of transaction cost and learning perspectives on alliance. Consistent with transaction-cost economics, the shift in purchase-supply relationships can be traced to changes in the nature of parts transactions and keiretsu -governance structures. A learning perspective on alliance complements and extends transaction-cost theory, providing additional explanation of the sources of change and the specific governance choices being made.
Our first two cases document a drift in Toyota's keiretsu supply network toward a hierarchical form in the management of parts-supply transactions. Toyota has effectively internalized its transactions with Daihatsu by taking a controlling interest. Toyota's strategy toward long-term partner Denso, on the other hand, was very different. Toyota built, from the ground up, an in-house capability in electronic components, thus scaling down its dependence on Denso. A third case considers a general trend in the Japanese auto industry toward greater standardization of parts. With the routinization of quality, reliability, and speed in supply management, the need for keiretsu -style governance has declined. The withering of keiretsu obligations is also traceable to globalization and the continuing weakness of the Japanese economy, which have prompted Japanese firms to question received business practice.
Journal Article