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Despite broad investigation in content-based image retrieval (CBIR), issue to lessen the semantic gap between high-level semantics and local attributes of the image is still an important issue. The local attributes of an image such as shape, color, and texture are not sufficient for effective CBIR. Visual similarity is a principal step in CBIR and in the baseline approach. In this article, we introduce a novel approach, which relies on the fusion of visual words of scale-invariant feature transform (SIFT) and binary robust invariant scalable keypoints (BRISK) descriptors based on the visual-bag-of-words approach. The two local feature descriptors are chosen as their fusion adds complementary improvement to CBIR. The SIFT descriptor is capable of detecting objects robustly under cluttering due to its invariance to scale, rotation, noise, and illumination variance.However, SIFT descriptor does not perform well at low illumination or poorly localized keypoints within an image. Due to this reason, the discriminative power of the SIFT descriptor is lost during the quantization process, which also reduces the performance of CBIR. However, the BRISK descriptor provides scale and rotation-invariant scale-space, high quality and adaptive performance in classification based applications. It also performs better at poorly localized keypoints along the edges of an object within an image as compared to the SIFT descriptor. The suggested approach based on the fusion of visual words achieves effective results on the Corel-1K, Corel-1.5K, Corel-5K, and Caltech-256 image repositories as equated to the feature fusion of both descriptors and latest CBIR approaches with the surplus assistances of scalability and fast indexing.

In recent years, the rapid growth of multimedia content makes content-based image retrieval (CBIR) a challenging research problem. The content-based attributes of the image are associated with the position of objects and regions within the image. The addition of image content-based attributes to image retrieval enhances its performance. In the last few years, the bag-of-visual-words (BoVW) based image representation model gained attention and significantly improved the efficiency and effectiveness of CBIR. In BoVW-based image representation model, an image is represented as an order-less histogram of visual words by ignoring the spatial attributes. In this paper, we present a novel image representation based on the weighted average of triangular histograms (WATH) of visual words. The proposed approach adds the image spatial contents to the inverted index of the BoVW model, reduces overfitting problem on larger sizes of the dictionary and semantic gap issues between high-level image semantic and low-level image features. The qualitative and quantitative analysis conducted on three image benchmarks demonstrates the effectiveness of the proposed approach based on WATH.

The mixed nano-ferrites materials Cu 0.6 Zn 0.4 Ho x Fe 2− x O 4 (0.00 ≤  x  ≤ 0.12) were prepared via the sol–gel auto combustion technique. The TGA curve established the annealing temperature (500 °C) for phase formation. The single exothermic peak on the DSC plot occurred at 341 °C temperature. XRD patterns of these nano ferrites verified single phase formation of the FCC cubic structure. The lattice constant a was increased from 8.4244 to 8.4419 Å and then its value decreased to 8.4319 Å. Crystallite size was found in the range of 7 to 16 nm. The surface morphology of the samples was observed from the scanning electron microscope (SEM) images. The grain size was found within the range of 90 nm to 106 nm. Energy-dispersive X-ray (EDX) results showed the significant substitution of Ho in Cu–Zn spinel ferrites. Dielectric parameters such as dielectric constant, dielectric constant factor, and tan loss depicted a decreasing trend with Ho 3+ ions concentration. The single-domain ferrimagnetism is confirmed by the squareness ratio, which is < 0.5. The M s was found to be decreased while H c was increased. The significant results of the dielectric as well as magnetic properties of these nano ferrites suggested their use in high-frequency devices applications.

The current study highlights prospective mechanisms of biogenesis of extracellular vesicles (EVs) and potential involvement in cellular signaling and transport with great emphasis to illustrate their role as biomarkers in certain pathologies. The current review highlights EVs, the heterogeneous entities secreted by cells in highly conserved manner. A series of consensus terminologies for various types is yet to be organized. Exosomes, microvesicles and apoptotic bodies are major populations among EVs. EVs are key regulators in cellular physiological homeostasis, disease progression and evolve either from plasma membrane (microvesicles) or fusion of endosomes with exosomes. However, how vesicular inclusions elicit a plethora of biological responses is still not much clear. However, how these vesicular inclusions get packaged and delivered by these EVs shows great involvement in inter- and intracellular cellular signaling and channeling of multiple proteins, variety of RNAs and certain fat molecules. It’s worth to mention that EVs carry small non-coding RNAs (snRNAs) which are involved in multiple cellular molecular events at targeted sites. Moreover, snRNA trafficking through exosomes and microvesicles depicts remarkable potential as non-invasive biomarkers in different clinical disorders especially immune system pathologies, cardiovascular issues, and metabolic syndromes.

From the past few decades, attention towards the biological evaluation of nanoparticles (NPs) has increased due to the persistent and extensive application of NPs in various fields, including biomedical science, modern industry, magnetic resonance imaging, and the construction of sensors. Therefore, in the current study, magnetic nickel ferrite (NiFe2O4) nanoparticles (NFNPs) were synthesized and evaluated for their possible adverse effects in rabbits. The crystallinity of the synthesized NFNPs was confirmed using X-ray diffraction (XRD) technique. The saturation magnetization (46.7 emug-1) was measured using vibrating sample magnetometer (VSM) and 0.35-tesla magnetron by magnetic resonance imaging (MRI). The adverse effects of NFNPs on blood biochemistry and histoarchitecture of the liver, kidneys, spleen, brain, and heart of the rabbits were determined. A total of sixteen adult rabbits, healthy and free from any apparent infection, were blindly placed in two groups. The rabbits in group A served as control, while the rabbits in group B received a single dose (via ear vein) of NFNPs for ten days. The blood and visceral tissues were collected from each rabbit at days 5 and 10 of posttreatment. The results on blood and serum biochemistry profile indicated significant variation in hematological and serum biomarkers in NFNP-treated rabbits. The results showed an increased quantity of oxidative stress and depletion of antioxidant enzymes in treated rabbits. Various serum biochemical tests exhibited significantly higher concentrations of different liver function tests, kidney function tests, and cardiac biomarkers. Histopathologically, the liver showed congestion, edema, atrophy, and degeneration of hepatocytes. The kidneys exhibited hemorrhages, atrophy of renal tubule, degeneration, and necrosis of renal tubules, whereas coagulative necrosis, neutrophilic infiltration, and severe myocarditis were seen in different sections of the heart. The brain of the treated rabbits revealed necrosis of neurons, neuron atrophy, and microgliosis. In conclusion, the current study results indicated that the highest concentration of NPs induced adverse effects on multiple tissues of the rabbits.

ABSTRACT Fractures related to long bones in female Beetal goats fail to heal or show delayed healing that leads to intensified morbidity. Bone marrow aspirate (BMA) has been suggested as an efficient biological adjuvant for healing long bone fractures. BMA comprises bone mesenchymal stem cells. This study aims to assess the potential of autologous BMA on metacarpal and metatarsal fracture of Beetal goats presented at the surgery clinic of the University of Veterinary and Animal Sciences Lahore, Pakistan. Beetal goats were selected (n=20), and divided into four different groups. The first group was designated as bone plating with bone marrow aspirate (BPMA) in which fracture was reduced by using the bone plates along with the application of multiple BMA on days: 0, 14, 28, and 45. Furthermore, the second group was designated as bone plating with normal saline (BPNS) in which fracture was reduced by using the bone plates along with the application of normal saline. Additionally, the third group was designated as bone wiring with bone marrow aspirate (BWBM), and the fourth group, was bone wiring with normal saline (BWNS). Both third and fourth groups were treated with bone wiring along with BMA and bone wiring along with NS respectively. The rate of healing post-treatment was assessed by radiographic union score (RUS), weight-bearing score (WBS), and serobiochemical evaluations on days: 0, 7, 14, 28, and 45. Our data showed a significant difference in the healing of fractures treated with BMA as compared to NS on days; 7 and 14. Moreover, the RUS, WBS, and serobiochemical profiles of goats treated with BMA showed improved healing of fractures as compared to the goats treated with NS. In summary, we observed that the healing process of the metacarpal and metatarsal fractured bones was reduced by bone plating, and bone wiring was ameliorated with the application of multiple BMA. We proposed further studies on larger cohorts. BMA may be used as supportive therapy to enhance the healing process of fractures in goats.

The market for nanoparticles has grown significantly over the past few decades due to a number of unique qualities, including antibacterial capabilities. It is still unclear how nanoparticle toxicity works. In order to ascertain the toxicity of synthetic cobalt iron oxide (CoFe2O4) nanoparticles (CIONPs) in rabbits, this study was carried out. Sixteen rabbits in total were purchased from the neighborhood market and divided into two groups (A and B), each of which contained eight rabbits. The CIONPs were synthesized by the co-precipitation method. Crystallinity and phase identification were confirmed by X-ray diffraction (XRD). The average size of the nanoparticles (13.2 nm) was calculated by Scherrer formula (Dhkl = 0.9 λ/β cos θ) and confirmed by TEM images. The saturation magnetization, 50.1 emug−1, was measured by vibrating sample magnetometer (VSM). CIONPs were investigated as contrast agents (CA) for magnetic resonance images (MRI). The relaxivity (r = 1/T) of the MRI was also investigated at a field strength of 0.35 T (Tesla), and the ratio r2/r1 for the CIONPs contrast agent was 6.63. The CIONPs were administrated intravenously into the rabbits through the ear vein. Blood was collected at days 5 and 10 post-exposure for hematological and serum biochemistry analyses. The intensities of the signal experienced by CA with CIONPs were 1427 for the liver and 1702 for the spleen. The treated group showed significantly lower hematological parameters, but significantly higher total white blood cell counts and neutrophils. The results of the serum biochemistry analyses showed significantly higher and lower quantities of different serum biochemical parameters in the treated rabbits at day 10 of the trial. At the microscopic level, different histological ailments were observed in the visceral organs of treated rabbits, including the liver, kidneys, spleen, heart, and brain. In conclusion, the results revealed that cobalt iron oxide (CoFe2O4) nanoparticles induced toxicity via alterations in multiple tissues of rabbits.

ABSTRACT A variety of infectious public health problems are prevailing in the world. Among these, epidemics of Severe Acute Respiratory Syndrome (SARS), H1N1 influenza and Middle East Respiratory Syndrome corona virus (MERS-Co V) emerged as very important issues during last three decades as these infections caused quite large number of human deaths worldwide. Coronaviruses are single-stranded positive sense RNA viruses which mainly in past were considered responsible for high percentage of (around 30%) of common cold/flu cases. Viruses causing SARS, MERS and COVID-19 are members of family Coronavirdae. World Health Organization (WHO) reported that the novel Cov-19 virus infection was first diagnosed in Wuhan, Hubei Province, China during December 2019. Initially the virus was named as nCoV-19 and later disease due to this virus was named as COVID-19, and recently named as Severe Acute Respiratory Syndrome Corona virus 2 (SARS-CoV-2) by the International Committee on Taxonomy of Viruses. This new coronavirus was found to have 86.9% homology to a bat corona virus and hence it was suspected to have been originated from bats. Till 15th January 2021, the COVID-19 infection has been reported from 219 countries. It has caused over 20 million deaths in humans around the globe. Countries reporting very high death/infection rates include USA (393,948/23,617,815), Brazil (206,009/5,257,459), Mexico (136,917/1,571,901), India (153,000/10,596,442), UK (84,767/3,211,576), France (69,031/2,830,842), Russia (63,940/3,495,816) and Italy (80,326/2,319,036). Worldwide a total of over 96,750,700 COVID-19 cases have so far been reported. As reported earlier this pandemic has hit almost every country worldwide causing exceptionally high morbidity and mortality. Amongst the South Asian countries India is worst hit by this deadly COVID-19. Pakistan's neighboring Iran is also very badly infected and reported 57,057 deaths of 1,348,316 infected people. In Pakistan 52,411,930 confirm cases of COVID-19 and around 11,000 deaths are reported in various region of the country.

The research study was designed to strengthen and better understand the learning skills in veterinary anatomy by using recent scientific skeleton preparation and preservation techniques. The study was conducted at the skeleton preparation section, anatomy laboratory, Department of Bio-Sciences, Faculty of Veterinary Sciences, Bahauddin Zakariya University Multan, Pakistan. Adult healthy / postmortem cow, horse, goats, dog and peacock, were used in this study. The internal soft tissue organs were kept in a 10% prepared formalin solution after the animals had been skinned and dissected. Boiling and maceration techniques were used to prepare the skeleton models and loose bones. Bones were first boiled in caustic soda for 4 to 8 hours and then degreased with gasoline for seven days for large animals and five days for small animals. After that, bones were left to dry naturally for a day. Then bones were bleached in a 4% hydrogen peroxide solution for two days. In the maceration technique, insect larvae were used to clean the bones but prolonged the time for better results. Bones were drilled and fixed with copper wire and adhesive material. The vertebral column and both limbs were supported by iron rods. Skeletons were assembled on the wooden base. These prepared skeleton models and loose bones were used to learn veterinary anatomy.