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Exocytosis is a key mechanism for delivering materials into the extracellular space for cell function and communication. In plant cells, conventional protein secretion (CPS) is achieved via an ER (endoplasmic reticulum)-Golgi-TGN (trans-Golgi network)-PM (plasma membrane) pathway. Unconventional protein secretion (UPS) bypassing these secretory organelles is also in operation and can potentially lead to the formation of extracellular vesicles (EVs) in plant cells. Although multiple types of EVs have been identified and shown to play important roles in mediating intercellular communications in mammalian cells, there has been a long debate about the possible existence of EVs in plants because of the presence of the cell wall. However, increasing evidence suggests that plants also release EVs having various functions including unconventional protein secretion, RNA transport, and defense against pathogens. In this review, we present an update on the current knowledge about the nature, secretory mechanism, and function of various types of EVs in plants. The key regulators involved in EV secretion are also summarized and discussed. We pay special attention to the function of EVs in plant defense and symbiosis.

Chloroplasts provide energy for all plants by producing sugar during photosynthesis. To adapt to various environmental and developmental cues, plants have developed specific strategies to control chloroplast homeostasis in plant cells, including chloroplast degradation during leaf senescence and the transition of chloroplasts into other types of plastids during the day-night cycle. In recent years, autophagy has emerged as an essential mechanism for selective degradation of chloroplast materials (also known as chlorophagy) in the vacuole. Different types of membrane structures have been implicated to involve in the delivery of distinct chloroplast contents. Here we provide a current overview on chlorophagy and discuss the possible chloroplast receptors and upstream signals in this process.

The polished image of netizens on social media can confer numerous benefits. Drawing on Goffman's Dramaturgical approach, this study analyzes the rationale and repercussions of crafting idealized personas on social media platforms. This approach is evident in the way individuals curate their online presence. Enhancing one's image can boost personal confidence in social interactions; however, a falsified portrayal can lead others astray, affecting social trust. In people’s daily lives, this understanding helps us to better comprehend and navigate interpersonal relationships through nuanced social interactions. The Internet cannot fully capture the essence of individuals, and attempts to gain a comprehensive understanding of a public figure solely through online means are often futile. For individuals, any received information should be thoroughly analyzed, moving beyond the idealized facade that others present, examining the underlying substance, and assimilating only credible and genuine information. By applying the theory of dramaturgy to analyze social images both on stage and behind the scenes, one can objectively assess the true intentions behind the creation of social personas. Those seeking greater benefits may resort to excessive packaging to deceive the public, necessitating more discernment and critical thinking in social interactions to prevent being misled.

The Yangtze River fishing ban policy is a central measure in China’s watershed governance, and the adaptability of its policy tools and collaborative mechanisms directly influences the sustainability and effectiveness of basin management. This study systematically examines the evolution of policy themes, the characteristics of policy tool combinations, and their alignment with intergovernmental collaborative governance needs, drawing on 120 central government policy texts issued between 2002 and 2024. Using frequency analysis and policy tool coding, the findings reveal that (1) policy themes have shifted from fishery resource control to comprehensive ecological protection and, more recently, to integrated watershed management, thereby driving progressively higher demands for intergovernmental collaboration. (2) The policy tool structure has long been dominated by environmental tools, supplemented by supply-side tools, while demand-side tools remain underdeveloped. Imbalances persist, such as excessive emphasis on resource inputs over capacity building in supply-side tools, rigid constraints with limited flexibility in environmental tools, and a reliance on publicity while underutilizing market incentives in demand-side tools. (3) Tool combinations have adapted to changing collaboration needs, evolving from rigid constraints and fiscal subsidies to institutional frameworks and cross-regional cooperation, ultimately forming a governance model characterized by systemic guarantees and diversified collaboration. Based on these findings, this study recommends strengthening long-term governance mechanisms, improving cross-regional collaborative structures, authorizing local governments to design context-specific implementation details, enhancing fishermen’s livelihood security and social development, expanding public participation and oversight, and exploring market mechanisms for realizing ecological product value. These measures aim to advance collaborative governance in the Yangtze River Basin and foster a balanced integration of ecological protection and social development.

Distinct pathways for protein secretion have been revealed in plant cells, both conventional and unconventional. Advanced techniques have been developed to unveil their underlying mechanisms. Abstract Protein secretion is an essential process in all eukaryotic cells and its mechanisms have been extensively studied. Proteins with an N-terminal leading sequence or transmembrane domain are delivered through the conventional protein secretion (CPS) pathway from the endoplasmic reticulum (ER) to the Golgi apparatus. This feature is conserved in yeast, animals, and plants. In contrast, the transport of leaderless secretory proteins (LSPs) from the cytosol to the cell exterior is accomplished via the unconventional protein secretion (UPS) pathway. So far, the CPS pathway has been well characterized in plants, with several recent studies providing new information about the regulatory mechanisms involved. On the other hand, studies on UPS pathways in plants remain descriptive, although a connection between UPS and the plant defense response is becoming more and more apparent. In this review, we present an update on CPS and UPS. With the emergence of new techniques, a more comprehensive understanding of protein secretion in plants can be expected in the future.

Being a major factory for protein synthesis, assembly, and export, the endoplasmic reticulum (ER) has a precise and robust ER quality control (ERQC) system monitoring its product line. However, when organisms are subjected to environmental stress, whether biotic or abiotic, the levels of misfolded proteins may overwhelm the ERQC system, tilting the balance between the capacity of and demand for ER quality control and resulting in a scenario termed ER stress. Intense or prolonged ER stress may cause damage to the ER as well as to other organelles, or even lead to cell death in extreme cases. To avoid such serious consequences, cells activate self-rescue programs to restore protein homeostasis in the ER, either through the enhancement of protein-folding and degradation competence or by alleviating the demands for such reactions. These are collectively called the unfolded protein response (UPR). Long investigated in mammalian cells and yeasts, the UPR is also of great interest to plant scientists. Among the three branches of UPR discovered in mammals, two have been studied in plants with plant homologs existing of the ER-membrane-associated activating transcription factor 6 (ATF6) and inositol-requiring enzyme 1 (IRE1). This review discusses the molecular mechanisms of these two types of UPR in plants, as well as the consequences of insufficient UPR, with a focus on experiments using model plants.

Increased crop yields are required to support rapid population growth worldwide. Grain weight is a key compo- nent of rice yield, but the underlying molecular mechanisms that control it remain elusive. Here, we report the clon- ing and characterization of a new quantitative trait locus （QTL） for the control of rice grain length, weight and yield. This locus, GL3.1, encodes a protein phosphatase kelch （PPKL） family -- Ser/Thr phosphatase. GL3.1 is a member of the large grain WY3 variety, which is associated with weaker dephosphorylation activity than the small grain FAZ1 variety. GL3.I-WY3 influences protein phosphorylation in the spikelet to accelerate cell division, thereby re- suiting in longer grains and higher yields. Further studies have shown that GL3.1 directly dephosphorylates its sub- strate, Cyclin-TI;3, which has only been rarely studied in plants. The downregulation of Cyclin-T1;3 in rice resulted in a shorter grain, which indicates a novel function for Cyclin-T in cell cycle regulation. Our findings suggest a new mechanism for the regulation of grain size and yield that is driven through a novel phosphatase-mediated process that affects the phosphorylation of Cyclin-T1;3 during cell cycle progression, and thus provide new insight into the mechanisms underlying crop seed development. We bred a new variety containing the natural GL3.1 allele that demonstrated increased grain yield, which indicates that GL3.1 is a powerful tool for breeding high-yield crops.

Small heat shock proteins (sHsps) bind unfolding proteins, thereby playing a pivotal role in the maintenance of proteostasis in virtually all living organisms. Structural elucidation of sHsp-substrate complexes has been hampered by the transient and heterogeneous nature of their interactions, and the precise mechanisms underlying substrate recognition, promiscuity, and chaperone activity of sHsps remain unclear. Here we show the formation of a stable complex between Arabidopsis thaliana plastid sHsp, Hsp21, and its natural substrate 1-deoxy-D-xylulose 5-phosphate synthase (DXPS) under heat stress, and report cryo-electron microscopy structures of Hsp21, DXPS and Hsp21-DXPS complex at near-atomic resolution. Monomeric Hsp21 binds across the dimer interface of DXPS and engages in multivalent interactions by recognizing highly dynamic structural elements in DXPS. Hsp21 partly unfolds its central α-crystallin domain to facilitate binding of DXPS, which preserves a native-like structure. This mode of interaction suggests a mechanism of sHsps anti-aggregation activity towards a broad range of substrates. Structural insights into the small heat shock proteins (sHsps) complexes with their substrates are sparse. Here, cryo-EM structure of a plastid sHsp, Hsp21, in complex with a bona fide substrate 1-deoxy-D-xylulose 5-phosphate synthase (DXPS), suggests the anti-aggregation mechanism employed by sHsps.

In this study, a novel pattern recognition method named boosting–partial least squares–discriminant analysis (Boosting-PLS-DA) was developed for the non-destructive and accurate origin identification of food and medicine homologous substances (FMHSs). Taking Gastrodia elata, Aurantii Fructus Immaturus, and Angelica dahurica as examples, spectra of FMHSs from different origins were obtained by portable near-infrared (NIR) spectroscopy without destroying the samples. The identification models were developed with Boosting-PLS-DA, compared with principal component analysis (PCA) and partial least squares–discriminant analysis (PLS-DA) models. The model performances were evaluated using the validation set and an external validation set obtained one month later. The results showed that the Boosting-PLS-DA method can obtain the best results. For the analysis of Aurantii Fructus Immaturus and Angelica dahurica, 100% accuracies of the validation sets and external validation sets were obtained using Boosting-PLS-DA models. For the analysis of Gastrodia elata, Boosting-PLS-DA models showed significant improvements in external validation set accuracies compared to PLS-DA, reducing the risk of overfitting. Boosting-PLS-DA method combines the high robustness of ensemble learning with the strong discriminative capability of discriminant analysis. The generalizability will be further validated with a sufficiently large external validation set and more types of FMHSs.

The Cao’e-Jiaojiang-Oujiang River Basin possesses abundant vernacular architectural heritage with significant historical–cultural value. However, challenges like dispersed distribution and inconsistent conservation hinder its systematic protection and utilization within territorial spatial planning, necessitating a deeper understanding of its spatiotemporal patterns. Utilizing 570 identified heritage sites, this study employed ArcGIS spatial analysis (Kernel Density Estimation, Nearest Neighbor Index), correlation analysis with DEM data, and suitability analysis (Minimum Cumulative Resistance model, Gravity Model) to systematically examine spatial distribution characteristics, their evolution, and relationships with the geographical environment and historical context. Results revealed a distinct “four cores and three belts” spatial pattern. Temporally, distribution evolved from “discrete” (Song-Yuan) to “aggregated” (Ming-Qing) and then “diffused” (Modern era). Spatially, heritage showed density in plains, preference for low slopes, and settlement along waterways. Suitability analysis indicated higher corridor potential in the northern section (Cao’e-Jiaojiang) than the south (Oujiang), leading to the identification of a “Northern Segment (Shaoxing-Ningbo-Shengzhou-Taizhou)” and “Southern Segment (Wenzhou-Lishui)” corridor structure. This research provides a scientific basis for systematic conservation and integrated heritage corridor construction of vernacular architectural heritage in the basin, supporting Zhejiang’s Poetry Road Cultural Belt initiatives and cultural heritage protection within territorial spatial planning.