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Wind energy production is rapidly growing in the United States and is expected to continue increasing as more and larger wind turbines are installed. To support these taller and heavier onshore turbines, new foundations must be designed and manufactured. One proposed method of reducing the total amount of concrete and steel in spread foundations is to utilize additive manufacturing to enable more material‐efficient designs. To compare these additively manufacturing‐enabled designs to conventional foundation designs, this study performs a life cycle impact assessment of four ultra‐tall wind turbine foundations: two foundations using 78‐MPa 3D printed stay‐in‐place concrete formwork cast with 35‐MPa ready‐mix concrete with reinforcements, and two conventional foundations cast entirely out of 35‐MPa concrete with reinforcements. The life cycle assessment investigates the environmental impacts of four different stages, including materials production, transportation, construction, and end‐of‐life. The materials production stage is found to dominate the life cycle results, contributing over 97% of the total CO2 emissions and over 88% of the fossil fuel depletion for each foundation. Compared to the conventional designs, the Short Flat Ribbed Beam foundation with 3D printed formwork has 22.4% lower CO2 emissions and 28.3% lower fossil fuel depletion than the Circular foundation, and 2.0% higher CO2 and 5.9% lower fossil fuel depletion compared to the Tapered foundation. Parametric studies indicate that reducing cement content and increasing recycled content in printed concrete can significantly reduce the overall life cycle impacts of the foundations.

A young girl, observing that life with her family is not easy, imagines the quintet as a pod of pelicans, a swarm of bees, or a herd of buffalo. Includes note about, and list of, collective nouns for animals.

To inform response strategies, we examined type 1 humoral and intestinal immunity induced by 1) one fractional inactivated poliovirus vaccine (fIPV) dose given with monovalent oral poliovirus vaccine (mOPV1), and 2) mOPV1 versus bivalent OPV (bOPV). We conducted a randomized, controlled, open-label trial in Dhaka, Bangladesh. Healthy infants aged 5 weeks were block randomized to one of four arms: mOPV1 at age 6–10–14 weeks/fIPV at 6 weeks (A); mOPV1 at 6–10–14 weeks/fIPV at 10 weeks (B); mOPV1 at 6–10–14 weeks (C); and bOPV at 6–10–14 weeks (D). Immune response at 10 weeks and cumulative response at 14 weeks was assessed among the modified intention-to-treat population, defined as seroconversion from seronegative (<1:8 titers) to seropositive (≥1:8) or a four-fold titer rise among seropositive participants sustained to age 18 weeks. We examined virus shedding after two doses of mOPV1 with and without fIPV, and after the first mOPV1 or bOPV dose. The trial is registered at ClinicalTrials.gov (NCT03722004). During 18 December 2018 – 23 November 2019, 1,192 infants were enrolled (arms A:301; B:295; C:298; D:298). Immune responses at 14 weeks did not differ after two mOPV1 doses alone (94% [95% CI: 91–97%]) versus two mOPV1 doses with fIPV at 6 weeks (96% [93–98%]) or 10 weeks (96% [93–98%]). Participants who received mOPV1 and fIPV at 10 weeks had significantly lower shedding (p < 0·001) one- and two-weeks later compared with mOPV1 alone. Response to one mOPV1 dose was significantly higher than one bOPV dose (79% versus 67%; p < 0·001) and shedding two-weeks later was significantly higher after mOPV1 (76% versus 56%; p < 0·001) indicating improved vaccine replication. Ninety-nine adverse events were reported, 29 serious including two deaths; none were attributed to study vaccines. Given with the second mOPV1 dose, fIPV improved intestinal immunity but not humoral immunity. One mOPV1 dose induced higher humoral and intestinal immunity than bOPV. U.S. Centers for Disease Control and Prevention.

This catalogue raisonnâe is the first study of this area of the Royal Collection for more than a hundred years. Extensive research has uncovered much new information relating to the European silver in the Collection since the last publication on the subject in the early twentieth century. The catalogue discusses more than 350 objects of pre-twentieth-century silver made in France, Germany, the Netherlands and Russia, with a smaller collection of pieces from Italy, Scandinavia, Spain, Portugal and the Austro-Hungarian empire. An introduction on the history of collecting European silver is followed by catalogue entries on silver objects used for dining and drinking, tea, coffee and chocolate wares; toilet services; desk accessories and church plate. Highlights include the German Kunstzkammer objects acquired by George IV, and items formerly belonging to Napoleon which have subsequently entered the Royal Collection.

Chagas disease, caused by the parasite Trypanosoma cruzi, develops into chronic Chagas' cardiomyopathy in ~30% of infected individuals, characterized by conduction disorders, arrhythmias, heart failure, and even sudden cardiac death. Current anti-parasitic treatments are plagued by significant side effects and poor efficacy in the chronic phase of disease; thus, there is a pressing need for new treatment options. A therapeutic vaccine could bolster the protective TH1-mediated immune response, thereby slowing or halting the progression of chronic Chagas' cardiomyopathy. Prior work in mice has demonstrated therapeutic efficacy of a Tc24 recombinant protein vaccine in the acute phase of Chagas disease. However, it is anticipated that humans will be vaccinated therapeutically when in the chronic phase of disease. This study investigates the therapeutic efficacy of a vaccine prototype containing recombinant protein Tc24, formulated with an emulsion containing the Toll-like receptor 4 agonist E6020 as an immunomodulatory adjuvant in a mouse model of chronic T. cruzi infection. Among outbred ICR mice vaccinated during chronic T. cruzi infection, there is a significant increase in the number of animals with undetectable systemic parasitemia (60% of vaccinated mice compared to 0% in the sham vaccine control group), and a two-fold reduction in cardiac fibrosis over the control group. The vaccinated mice produce a robust protective TH1-biased immune response to the vaccine, as demonstrated by a significant increase in antigen-specific IFNγ-production, the number of antigen-specific IFNγ-producing cells, and IgG2a antibody titers. Importantly, therapeutic vaccination significantly reduced cardiac fibrosis in chronically infected mice. This is a first study demonstrating therapeutic efficacy of the prototype Tc24 recombinant protein and E6020 stable emulsion vaccine against cardiac fibrosis in a mouse model of chronic T. cruzi infection.

Nine stories featuring artwork by Richard Scarry include such tales as \"Chicken Little,\" \"The Country Mouse and the City Mouse,\" and \"The Gingerbread Man.\"

The Bermuda Principles for DNA sequence data sharing are an enduring legacy of the Human Genome Project (HGP). They were adopted by the HGP at a strategy meeting in Bermuda in February of 1996 and implemented in formal policies by early 1998, mandating daily release of HGP-funded DNA sequences into the public domain. The idea of daily sharing, we argue, emanated directly from strategies for large, goal-directed molecular biology projects first tested within the \"community\" of C. elegans researchers, and were introduced and defended for the HGP by the nematode biologists John Sulston and Robert Waterston. In the C. elegans community, and subsequently in the HGP, daily sharing served the pragmatic goals of quality control and project coordination. Yet in the HGP human genome, we also argue, the Bermuda Principles addressed concerns about gene patents impeding scientific advancement, and were aspirational and flexible in implementation and justification. They endured as an archetype for how rapid data sharing could be realized and rationalized, and permitted adaptation to the needs of various scientific communities. Yet in addition to the support of Sulston and Waterston, their adoption also depended on the clout of administrators at the US National Institutes of Health (NIH) and the UK nonprofit charity the Wellcome Trust, which together funded 90% of the HGP human sequencing effort. The other nations wishing to remain in the HGP consortium had to accommodate to the Bermuda Principles, requiring exceptions from incompatible existing or pending data access policies for publicly funded research in Germany, Japan, and France. We begin this story in 1963, with the biologist Sydney Brenner's proposal for a nematode research program at the Laboratory of Molecular Biology (LMB) at the University of Cambridge. We continue through 2003, with the completion of the HGP human reference genome, and conclude with observations about policy and the historiography of molecular biology.

Axenically cultured Liberibacter crescens (Lcr) is a closely related surrogate for uncultured plant pathogenic species of the genus Liberibacter, including ‘ Candidatus L. asiaticus’ (CLas) and ‘ Ca . L. solanacearum’ (CLso). All Liberibacters encode a completely conserved gene repertoire for both flagella and Tad ( T ight Ad herence) pili and all are missing genes critical for nucleotide biosynthesis. Both flagellar swimming and Tad pilus-mediated twitching motility in Lcr were demonstrated for the first time. A role for Tad pili in the uptake of extracellular dsDNA for food in Liberibacters was suspected because both twitching and DNA uptake are impossible without repetitive pilus extension and retraction, and no genes encoding other pilus assemblages or mechanisms for DNA uptake were predicted to be even partially present in any of the 35 fully sequenced Liberibacter genomes. Insertional mutations of the Lcr Tad pilus genes cpaA , cpaB , cpaE , cpaF and tadC all displayed such severely reduced growth and viability that none could be complemented. A mutation affecting cpaF (motor ATPase) was further characterized and the strain displayed concomitant loss of twitching, viability and reduced periplasmic uptake of extracellular dsDNA. Mutations of comEC , encoding the inner membrane competence channel, had no effect on either motility or growth but completely abolished natural transformation in Lcr. The comEC mutation was restored by complementation using comEC from Lcr but not from CLas strain psy62 or CLso strain RS100, indicating that unlike Lcr, these pathogens were not naturally competent for transformation. This report provides the first evidence that the Liberibacter Tad pili are dynamic and essential for both motility and DNA uptake, thus extending their role beyond surface adherence.

Key Points Symbiotic nitrogen-fixing rhizobial bacteria and leguminous plants have evolved complex signal exchange mechanisms that allow a specific bacterial species to induce its host plant to form invasion structures through which it enters the plant root. Once these invasion structures reach the target cells in the interior of the plant root, the bacteria are endocytosed within a host cell membrane-derived compartment. In the microaerobic environment provided by the host cell, the bacteria differentiate into a specialized form called a bacteroid. The bacteroid form expresses the oxygen-sensitive enzyme nitrogenase that catalyzes the conversion of atmospheric nitrogen to ammonia. The dissection of the bacterial and plant signalling pathways that are involved in each stage of the invasion process has been facilitated by the complete genomic sequencing of Sinorhizobium meliloti and the near complete sequencing of the genome of the model host plant Medicago truncatula . Rhizobial bacteria interact very differently with the plant innate immune system than other groups of bacteria. Rhizobia lack some of the microbial molecular patterns that provoke plant defence responses. Additionally, legume plants differ from other plant families in that they lack the ability to perceive and respond defenceively to other microbial molecular patterns. Symbiotic rhizobial bacteria are similar to pathogenic bacteria such as Brucella spp, in that they both form chronic infections of eukaryotic cells within a host-derived membrane compartment, and require some of the same bacterial factors for survival within the host. These factors include the correct structure of the lipopolysaccharide core and lipid A, presence of cyclic β-glucans, and a common bacterial regulatory circuitry. The symbiotic relationship between leguminous plants and rhizobial bacteria is one of the most well-studied microbial symbioses. The availability of genome sequence information for many of the bacterial and plant partners involved has been invaluable and in this article, the authors review the most recent discoveries about the mutual recognition between Sinorhizobium meliloti and Medicago truncatula . Nitrogen-fixing rhizobial bacteria and leguminous plants have evolved complex signal exchange mechanisms that allow a specific bacterial species to induce its host plant to form invasion structures through which the bacteria can enter the plant root. Once the bacteria have been endocytosed within a host-membrane-bound compartment by root cells, the bacteria differentiate into a new form that can convert atmospheric nitrogen into ammonia. Bacterial differentiation and nitrogen fixation are dependent on the microaerobic environment and other support factors provided by the plant. In return, the plant receives nitrogen from the bacteria, which allows it to grow in the absence of an external nitrogen source. Here, we review recent discoveries about the mutual recognition process that allows the model rhizobial symbiont Sinorhizobium meliloti to invade and differentiate inside its host plant alfalfa ( Medicago sativa ) and the model host plant barrel medic ( Medicago truncatula ).