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The present study was aimed to biosynthesis of silver nanoparticles by using rhaminolipid produced from local isolate Pseudomonas aeruginosa as reducing and stabilizing agent. Silver nanoparticles (AgNPs) synthesized by green method have shown several applications such as biomedical, anticancer, bio sensing, catalysis etc. Characterization study of purified bioemulsifier using thin layer chromatography (TLC) was demonstrated that the biosurfactant contains mono, and di- rhamnolipid with Rf values of 0.86 and 0.36 respectively. Optimization results of biosynthesis silver nanoparticles were revealed that an increasing in intensity of Surface Plasmon Resonance (SPR) bands of nanoparticles with shifting at wavelength (400 nm). Also optimum synthesis of AgNPs was at pH 5, Temperature 40℃, reaction time 5 minutes with concentration of rhaminolipid as reducing agents (2×10-3w/v) and Silver ion concentration (6×10-3 mol/L). The result of X-ray diffraction was indicated that the size of silver nanoparticles observed was 38 nm and show relatively stable peak at -23.2 mV. Finally, the minimum inhibitory concentration of Ag NPs against human pathogenic bacteria obtained at concentration (1mg/ml) for both gram negative and gram-positive bacteria. The results of antiinflammatory effects of Ag NPs obviously, cleared that the infection of test animals treated with AgNPs were completely healed after 6 days of treatment, while the animals treated with fucidin (as control) not exhibited any healing in the infection.

This study was depend to select Pseudomonas aeruginosa A3 as a good producer of rhamnolipid (RL) biosurfactant after screening on agar plate where it was able to biosynthesize 4.3 g/L with emulsification index 52% and reducing the surface tension of water to 33.2 mN/m. Therefore, this study was aimed to increase the production of biosurfactant from selected isolate by exposure to several physical and chemical mutagenic factors like gamma radiation, nitrosoguanidine, ethyl methane sulfonate and lithium chloride. The results were shown that 0.2 g/L of nitrosoguanidine was the best mutant for increasing the production to about 2 folds (9.4 g/L) after 15 mins exposure to this material, as well as the emulsification index and surface tension of water were reached to 62% and 26.1 mN/m, respectively, comparing with non-mutant isolate. Also, the critical micelle concentration (CMC) and critical micelle dilution (CMD) of produced rhamnolipid were reached to 120 mg/L and 100 fold, respectively. The optimum conditions of RL production from mutant isolate were determined as 34ºC a best temperature, 6.5 optimum pH and incubation period of 108 h where the production was reached to 10.6 g/L and emulsification index 64% with surface tension of water 26 mN/m. characterization study of purified RL by using thin layer chromatography (TLC) analysis indicated that it was composed of a mixture of mono and dirhamnolipid.