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ABSTRACT Background Although high‐dose biotin interference in automated immunoassays is now considered, there are very few studies showing biotin interference in manually operated research kits, especially with enzyme‐linked immunosorbent assay (ELISA). The aims of our study were to determine the effects of biotin interference on various parameters, including leptin, leptin receptor (LEPR), ghrelin, acylated ghrelin, deacylated ghrelin, ghrelin receptor (GHSR), kisspeptin (KISS1), kisspeptin receptor (KISS1R), preptin, peroxisome proliferator activated receptor gamma (PPARγ), nod‐like receptor pyrin domain‐containing 3 (NLRP3) and interleukin‐18 (IL‐18), which contribute to energy homeostasis in healthy and obese children. Methods Serum pools were prepared from healthy and obese individuals, and biotin concentrations in samples containing different amounts of biotin were measured via sandwich and competitive ELISA methods. In addition, possible biotin interactions were investigated by determining the concentrations of all the study parameters in serum pools containing different amounts of biotin. Results We found that the biotin‐competitive, ghrelin‐competitive, KISS1‐competitive, GHSR, leptin and LEPR ELISA kits were less affected by biotin interference and the results of these assay kits were more reliable. Unexpectedly, high levels were also measured in the biotin sandwich ELISA kit, indicating that biotin interference can also occur in manually operated assay kits. Conclusions Biotin exhibited an interference effect even in well‐functioning, qualified kits, and this negative effect was less common in competitive kits. Biotin interference was closely associated with the quality of the research kit, the parameters studied, and the presence of high biotin concentrations in the blood. In this study, we aimed for the first time to demonstrate biotin interference in ELISA research kits used in the analysis of pediatric obesity parameters other than routine testing. Our study revealed that biotin interaction may occur in manual ELISA kits and may cause erroneous test results. It will raise awareness among health professionals, researchers, and medical companies on this issue. By encouraging manufacturers developing medical products to take measures to reduce the effect of biotin interaction, it will prevent erroneous results in scientific studies and contribute to more precise measurements.

Background Childhood obesity leads to an increased inflammatory response, which in the long term can lead to serious health problems such as metabolic syndrome, diabetes, cancer and cardiovascular disease, and may also increase the risk of obesity in adulthood. Our aim was to evaluate inflammatory markers associated with pediatric obesity in 30 healthy children (BMI between the 15th–85th percentiles) and 30 obese children (BMI > 95th percentile) and to determine the possible associations of these markers. Methods Anthropometric measurements of the children were obtained. The serum preptin, peroxisome proliferator activated receptor gamma (PPARγ), nod-like receptor pyrin domain-containing 3 (NLRP3), and interleukin-18 (IL-18) levels of thirty children with obesity and thirty healthy children were determined using ELISA. Results NLPR3, preptin, and PPARγ levels were greater obese children than in healthy children ( p  < 0.05). We have shown that high PPARγ levels may have an inhibitory effect on the inflammatory activation of NLRP3. Conclusions In our study, the changes observed in preptin, NLRP3 and PPARγ parameters were found to be closely associated with pediatric obesity. These molecules may play an important role in understanding the relationship between pediatric obesity and inflammation and may be used as biomarkers in determining obesity treatment and complications in future studies.

Background Childhood obesity leads to an increased inflammatory response, which in the long term can lead to serious health problems such as metabolic syndrome, diabetes, cancer and cardiovascular disease, and may also increase the risk of obesity in adulthood. Our aim was to evaluate inflammatory markers associated with pediatric obesity in 30 healthy children (BMI between the 15th-85th percentiles) and 30 obese children (BMI > 95th percentile) and to determine the possible associations of these markers. Methods Anthropometric measurements of the children were obtained. The serum preptin, peroxisome proliferator activated receptor gamma (PPAR[gamma]), nod-like receptor pyrin domain-containing 3 (NLRP3), and interleukin-18 (IL-18) levels of thirty children with obesity and thirty healthy children were determined using ELISA. Results NLPR3, preptin, and PPAR[gamma] levels were greater obese children than in healthy children (p < 0.05). We have shown that high PPAR[gamma] levels may have an inhibitory effect on the inflammatory activation of NLRP3. Conclusions In our study, the changes observed in preptin, NLRP3 and PPAR[gamma] parameters were found to be closely associated with pediatric obesity. These molecules may play an important role in understanding the relationship between pediatric obesity and inflammation and may be used as biomarkers in determining obesity treatment and complications in future studies. Keywords: Child, Obesity, Preptin, NLRP3, PPAR[gamma]