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[This corrects the article DOI: 10.1371/journal.pone.0117654.].[This corrects the article DOI: 10.1371/journal.pone.0117654.].

Ovarian carcinoma (OC) is the most lethal gynecological malignancy. Despite the advances in the treatment of OC with combinatorial regimens, including surgery and platinum-based chemotherapy, patients generally exhibit poor prognosis due to high chemotherapy resistance. Herein, we tested the hypothesis that DNA damage response (DDR) pathways are involved in resistance of OC patients to platinum chemotherapy. Selected DDR signals were evaluated in two human ovarian carcinoma cell lines, one sensitive (A2780) and one resistant (A2780/C30) to platinum treatment as well as in peripheral blood mononuclear cells (PBMCs) from OC patients, sensitive (n = 7) or resistant (n = 4) to subsequent chemotherapy. PBMCs from healthy volunteers (n = 9) were studied in parallel. DNA damage was evaluated by immunofluorescence γH2AX staining and comet assay. Higher levels of intrinsic DNA damage were found in A2780 than in A2780/C30 cells. Moreover, the intrinsic DNA damage levels were significantly higher in OC patients relative to healthy volunteers, as well as in platinum-sensitive patients relative to platinum-resistant ones (all P<0.05). Following carboplatin treatment, A2780 cells showed lower DNA repair efficiency than A2780/C30 cells. Also, following carboplatin treatment of PBMCs ex vivo, the DNA repair efficiency was significantly higher in healthy volunteers than in platinum-resistant patients and lowest in platinum-sensitive ones (t1/2 for loss of γH2AX foci: 2.7±0.5h, 8.8±1.9h and 15.4±3.2h, respectively; using comet assay, t1/2 of platinum-induced damage repair: 4.8±1.4h, 12.9±1.9h and 21.4±2.6h, respectively; all P<0.03). Additionally, the carboplatin-induced apoptosis rate was higher in A2780 than in A2780/C30 cells. In PBMCs, apoptosis rates were inversely correlated with DNA repair efficiencies of these cells, being significantly higher in platinum-sensitive than in platinum-resistant patients and lowest in healthy volunteers (all P<0.05). We conclude that perturbations of DNA repair pathways as measured in PBMCs from OC patients correlate with the drug sensitivity of these cells and reflect the individualized response to platinum-based chemotherapy.

Ovarian carcinoma (OC) is the most lethal gynecological malignancy. Despite the advances in the treatment of OC with combinatorial regimens, including surgery and platinum-based chemotherapy, patients generally exhibit poor prognosis due to high chemotherapy resistance. Herein, we tested the hypothesis that DNA damage response (DDR) pathways are involved in resistance of OC patients to platinum chemotherapy. Selected DDR signals were evaluated in two human ovarian carcinoma cell lines, one sensitive (A2780) and one resistant (A2780/C30) to platinum treatment as well as in peripheral blood mononuclear cells (PBMCs) from OC patients, sensitive (n = 7) or resistant (n = 4) to subsequent chemotherapy. PBMCs from healthy volunteers (n = 9) were studied in parallel. DNA damage was evaluated by immunofluorescence gamma H2AX staining and comet assay. Higher levels of intrinsic DNA damage were found in A2780 than in A2780/C30 cells. Moreover, the intrinsic DNA damage levels were significantly higher in OC patients relative to healthy volunteers, as well as in platinum-sensitive patients relative to platinum-resistant ones (all P<0.05). Following carboplatin treatment, A2780 cells showed lower DNA repair efficiency than A2780/C30 cells. Also, following carboplatin treatment of PBMCs ex vivo, the DNA repair efficiency was significantly higher in healthy volunteers than in platinum-resistant patients and lowest in platinum-sensitive ones (t1/2 for loss of gamma H2AX foci: 2.7 plus or minus 0.5h, 8.8 plus or minus 1.9h and 15.4 plus or minus 3.2h, respectively; using comet assay, t1/2 of platinum-induced damage repair: 4.8 plus or minus 1.4h, 12.9 plus or minus 1.9h and 21.4 plus or minus 2.6h, respectively; all P<0.03). Additionally, the carboplatin-induced apoptosis rate was higher in A2780 than in A2780/C30 cells. In PBMCs, apoptosis rates were inversely correlated with DNA repair efficiencies of these cells, being significantly higher in platinum-sensitive than in platinum-resistant patients and lowest in healthy volunteers (all P<0.05). We conclude that perturbations of DNA repair pathways as measured in PBMCs from OC patients correlate with the drug sensitivity of these cells and reflect the individualized response to platinum-based chemotherapy.

Agricultural robotics has been a popular subject in recent years from an academic as well as a commercial point of view. This is because agricultural robotics addresses critical issues such as seasonal shortages in manual labor, e.g., during harvest, as well as the increasing concern regarding environmentally friendly practices. On one hand, several individual agricultural robots have already been developed for specific tasks (e.g., for monitoring, spraying, harvesting, transport, etc.) with varying degrees of effectiveness. On the other hand, the use of cooperative teams of agricultural robots in farming tasks is not as widespread; yet, it is an emerging trend. This paper presents a comprehensive overview of the work carried out so far in the area of cooperative agricultural robotics and identifies the state-of-the-art. This paper also outlines challenges to be addressed in fully automating agricultural production; the latter is promising for sustaining an increasingly vast human population, especially in cases of pandemics such as the recent COVID-19 pandemic.

This work pursues the potential of extending “Industry 4.0” practices to farming toward achieving “Agriculture 4.0”. Our interest is in fruit harvesting, motivated by the problem of addressing the shortage of seasonal labor. In particular, here we present an integrated system architecture of an Autonomous Robot for Grape harvesting (ARG). The overall system consists of three interdependent units: (1) an aerial unit, (2) a remote-control unit and (3) the ARG ground unit. Special attention is paid to the ARG; the latter is designed and built to carry out three viticultural operations, namely harvest, green harvest and defoliation. We present an overview of the multi-purpose overall system, the specific design of each unit of the system and the integration of all subsystems. In addition, the fully sensory-based sensing system architecture and the underlying vision system are analyzed. Due to its modular design, the proposed system can be extended to a variety of different crops and/or orchards.

Grapevine pruning is conducted during winter, and it is a very important and expensive task for wine producers managing their vineyard. During grapevine pruning every year, the past year’s canes should be removed and should provide the possibility for new canes to grow and produce grapes. It is a difficult procedure, and it is not yet fully automated. However, some attempts have been made by the research community. Based on the literature, grapevine pruning automation is approximated with the help of computer vision and image processing methods. Despite the attempts that have been made to automate grapevine pruning, the task remains hard for the abovementioned domains. The reason for this is that several challenges such as cane overlapping or complex backgrounds appear. Additionally, there is no public image dataset for this problem which makes it difficult for the research community to approach it. Motivated by the above facts, an image dataset is proposed for grapevine canes’ segmentation for a pruning task. An experimental analysis is also conducted in the proposed dataset, achieving a 67% IoU and 78% F1 score in grapevine cane semantic segmentation with the U-net model.