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In humans, peripheral sensory stimulation inhibits subsequent motor evoked potentials (MEPs) induced by transcranial magnetic stimulation; this process is referred to as short- or long-latency afferent inhibition (SAI or LAI, respectively), depending on the inter-stimulus interval (ISI) length. Although upper limb SAI and LAI have been well studied, lower limb SAI and LAI remain under-investigated. Here, we examined the time course of the soleus (SOL) muscle MEP following electrical tibial nerve (TN) stimulation at the popliteal fossa at ISIs of 20–220 ms. When the conditioning stimulus intensity was three-fold the perceptual threshold, MEP amplitudes were inhibited at an ISI of 220 ms, but not at shorter ISIs. TN stimulation just below the Hoffman (H)-reflex threshold intensity inhibited MEP amplitudes at ISIs of 30, 35, 100, 180 and 200 ms. However, the relationship between MEP inhibition and the P30 latency of somatosensory evoked potentials (SEPs) did not show corresponding ISIs at the SEP P30 latency that maximizes MEP inhibition. To clarify whether the site of afferent-induced MEP inhibition occurs at the cortical or spinal level, we examined the time course of SOL H-reflex following TN stimulation. H-reflex amplitudes were not significantly inhibited at ISIs where MEP inhibition occurred but at an ISI of 120 ms. Our findings indicate that stronger peripheral sensory stimulation is required for lower limb than for upper limb SAI and LAI and that lower limb SAI and LAI are of cortical origin. Moreover, the direct pathway from the periphery to the primary motor cortex may contribute to lower limb SAI.

Underwater walking is one of the most common hydrotherapeutic exercises. Therefore, understanding muscular control during underwater walking is important for optimizing training regimens. The effects of the water environment on walking are mainly related to the hydrostatic and hydrodynamic theories of buoyancy and drag force. To date, muscular control during underwater walking has been investigated at the individual muscle level. However, it is recognized that the human nervous system modularly controls multiple muscles through muscle synergies, which are sets of muscles that work together. We found that the same set of muscle synergies was shared between the two walking tasks. However, some task-dependent modulation was found in the activation combination across muscles and temporal activation patterns of the muscle synergies. The results suggest that the human nervous system modulates activation of lower-limb muscles during water walking by finely tuning basic locomotor muscle synergies that are used during land walking to meet the biomechanical requirements for walking in the water environment.

The extent to which muscle synergies represent the neural control of human behavior remains unknown. Here, we tested whether certain sets of muscle synergies that are fundamentally necessary across behaviors exist. We measured the electromyographic activities of 26 muscles, including bilateral trunk and lower limb muscles, during 24 locomotion, dynamic and static stability tasks, and we extracted the muscle synergies using non-negative matrix factorization. Our results show that 13 muscle synergies that may have unique functional roles accounted for almost all 24 tasks by combinations of single and/or merging of synergies. Therefore, our results may support the notion of the low dimensionality in motor outputs, in which the central nervous system flexibly recruits fundamental muscle synergies to execute diverse human behaviors. Further studies are required to validate the neural representation of the fundamental components of muscle synergies.

Subsurface limestone beds (SLBs) are used as a passive treatment technique to remove toxic metals from acid mine drainage (AMD). In this study, we investigated the mechanisms and thermodynamics of metal (manganese, copper, zinc, cadmium, and lead) precipitation in the SLB installed at the Motokura Mine. Field surveys in 2017 and 2018 showed that the pH of the SLB influent (initially 5–6) increased to approximately 8 in the drain between 24 and 45 m from the inlet. This increase was caused by limestone dissolution and resulted in the precipitation of hydroxides and/or carbonates of copper, zinc, and lead, as expected from theoretical calculations. Manganese and cadmium were removed within a pH range of approximately 7–8, which was lower than the pH at which they normally precipitate as hydroxides (pH 9–10). X-ray absorption near-edge structure analysis of the sediment indicated that δ-MnO 2 , which has a high cation-exchange capacity, was the predominant tetravalent manganese compound in the SLB rather than trivalent compound (MnOOH). Biological analysis indicates that microorganism activity of the manganese-oxidizing bacteria in the SLB provided an opportunity for δ-MnO 2 formation, after which cadmium was removed by surface complexation with MnO 2 (≡ MnOH 0  + Cd 2+  ⇄  ≡ MnOCd +  +  H + ). These findings show that biological agents contributed to the precipitation of manganese and cadmium in the SLB, and suggest that their utilization could enhance the removal performance of the SLB.

Cordyceps militaris produces cordycepin, which is known to be a bioactive compound. Currently, cordycepin hyperproduction of C. militaris was carried out in a liquid surface culture because of its low productivity in a submerged culture, however the reason was not known. In this study, 4.92 g/L of cordycepin was produced at the 15th day of C. militaris NBRC 103752 liquid surface culture, but only 1 mg/L was produced in the submerged culture. RNA-Seq was used to clarify the gene expression profiles of the cordycepin biosynthetic pathways of the submerged culture and the liquid surface culture. From this analysis, 1036 genes were shown to be upregulated and 557 genes were downregulated in the liquid surface culture compared with the submerged culture. Specifically, adenylosuccinate synthetase and phosphoribosylaminoimidazole-succinocarboxamide (SAICAR) synthase in purine nucleotide metabolism were significantly upregulated in the liquid surface culture. Thick mycelia formation in the liquid surface culture was found to induce the expression of hypoxia-related genes (GABA shunt, glutamate synthetase precursor, and succinate-semialdehyde dehydrogenase). Cytochrome P450 oxidoreductases containing heme were also found to be significantly enriched, suggesting that a hypoxic condition might be created in the liquid surface culture. These results suggest that hypoxic conditions are more suitable for cordycepin production in the liquid surface culture compared with the submerged culture. Our analysis paves the way for unraveling the cordycepin biosynthesis pathway and for improving cordycepin production in C. militaris.

It is well known that contracting the upper limbs can affect spinal reflexes of the lower limb muscle, via intraneuronal networks within the central nervous system. However, it remains unknown whether neuromuscular electrical stimulation (NMES), which can generate muscle contractions without central commands from the cortex, can also play a role in such inter-limb facilitation. Therefore, the objective of this study was to compare the effects of unilateral upper limb contractions using NMES and voluntary unilateral upper limb contractions on the inter-limb spinal reflex facilitation in the lower limb muscles. Spinal reflex excitability was assessed using transcutaneous spinal cord stimulation (tSCS) to elicit responses bilaterally in multiple lower limb muscles, including ankle and thigh muscles. Five interventions were applied on the right wrist flexors for 70 s: (1) sensory-level NMES; (2) motor-level NMES; (3) voluntary contraction; (4) voluntary contraction and sensory-level NMES; (5) voluntary contraction and motor-level NMES. Results showed that spinal reflex excitability of ankle muscles was facilitated bilaterally during voluntary contraction of the upper limb unilaterally and that voluntary contraction with motor-level NMES had similar effects as just contracting voluntarily. Meanwhile, motor-level NMES facilitated contralateral thigh muscles, and sensory-level NMES had no effect. Overall, our results suggest that inter-limb facilitation effect of spinal reflex excitability in lower limb muscles depends, to a larger extent, on the presence of the central commands from the cortex during voluntary contractions. However, peripheral input generated by muscle contractions using NMES might have effects on the spinal reflex excitability of inter-limb muscles via spinal intraneuronal networks.

White spot syndrome virus (WSSV) is one of the most devastating pathogens in penaeid shrimp and can cause massive damage in shrimp aquaculture industries. Previously, the WSSV structural protein VP15 was identified as an antigenic reagent against WSSV infections. In this study, we truncated this protein into VP15 (1–25) , VP15 (26–57) , VP15 (58–80) , and VP15 (1–25,58–80) . The purified proteins from the E. coli expression system were assayed as potential protective agents in Kuruma shrimp ( Marsupenaeus japonicus ) using the prime-and-boost strategy. Among the four truncated constructs, VP15 (26–57) provided a significant improvement in the shrimp survival rate after 20 days of viral infection. Subsequently, four peptides (KR11, SR11, SK10, and KK13) from VP15 (26–57) were synthesized and applied in an in vivo assay. Our results showed that SR11 could significantly enhance the shrimp survival rate, as determined from the accumulated survival rate. Moreover, a multiligand binding protein with a role in the host immune response and a possible VP15-binding partner, MjgC1qR, from the host M. japonicus were employed to test its binding with the VP15 protein. GST pull-down assays revealed that MjgC1qR binds with VP15, VP15 (26–57) , and SR11. Taken together, we conclude that SR11 is a determinant antigenic peptide of VP15 conferring antiviral activity against WSSV.

When discrepancies between planned and actual movements arise due to environmental changes, humans adjust movement parameters to achieve task goals. While motor adaptation has been extensively studied, the mechanisms involved in redundant movement parameters remain unclear. Split-belt treadmill adaptation, where each belt moves at a different speed, is an example of this phenomenon. Such adaptation initially induces gait asymmetry, which diminishes over time. Previous studies have postulated step length asymmetry as the target function; however, recent evidence challenges this assumption, leaving the target function undefined. This study investigates the target function by analyzing step parameter asymmetry using the goal-equivalent manifold and generalization predictability. The goal-equivalent manifold assesses whether adaptation is close to optimal in minimizing step parameter asymmetry, while generalization predictability reflects adaptation effects across different contexts, indicating potential target functions. We propose that step velocity asymmetry, rather than step length asymmetry, serves as the target function in split-belt treadmill adaptation. This framework facilitates the prediction and interpretation of both the learning process and the transfer of learning effects from trained to untrained conditions. In addition, it explains the overadaptation of step length asymmetry and the achievement of energy-efficient gait after adaptation. Therefore, we propose that step velocity asymmetry is the primary target function in split-belt treadmill adaptation.

Long-term adapted cell wall-deficient (L-forms) bacteria show unique cell shapes and growth patterns compared to wild-type bacteria. However, quantitative analysis to assess the morphological heterogeneity of existing L-forms is limited. In this study, we validated that two stable L-form strains of Escherichia coli (NC-7 and LWF+) hold spherical or pleomorphic morphology in confocal and electron microscopy. Using imaging flow cytometry, we further reported that the variations in cell size distribution and cell viability between L-forms and walled cells are statistically significant. Moreover, freeze-fracture electron microscopy observations revealed a clear presence of an outer membrane in NC-7 but not in LWF+, suggesting that E. coli L-form strains could survive in both spheroplastic and protoplastic forms after adaptive evolution. Accordingly, the mutations in genes associated with cell envelope and outer membrane components are more prevalent in the LWF+ genome, potentially leading to outer membrane depletion. Notably, experimental evidence derived from E. coli LWF+ cells exhibiting a monoderm phenotype may support the diderm-to-monoderm transition hypothesis, implying the monoderm phenotype arose from the evolutionary loss of the outer membrane in diderm ancestors. Taken together, our findings offer insights into quantification analysis and the cell envelope status of two E. coli L-forms, facilitating future investigations into genotype-phenotype associations in these two L-form bacteria models.

INTRODUCTION: There is no consensus regarding surgery and endotracheal treatment for bronchial stenosis. We report a case of right main bronchus stenosis treated using a novel approach of mediastinal mobilization with pericardial suspension.CASE PRESENTATION: A 57-year-old woman who developed radiation pneumonitis after postoperative radiation therapy for left breast cancer was admitted to our hospital due to worsening respiratory distress over 2 years. Chest CT revealed severe stenosis of the right main bronchus owing to lung destruction and scoliosis. Although isolated lung ventilation using a double-lumen tube was initiated, the ventilation was unstable. The patient underwent surgery, including sternal elevation using the Nuss technique, mediastinal mobilization using pericardial suspension, and tracheobronchial stenting to ameliorate bronchial stenosis.CONCLUSIONS: After surgery, oxygenation was no longer required. This novel pericardial suspension technique fundamentally corrects the extrinsic mediastinal shift, serving as an essential prerequisite for safe stenting against secondary tracheobronchomalacia in adult cases.