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Three novel hypoxanthine derivatives (1–3) were obtained from the Okinawan cyanobacterium Okeania hirsuta. The structures of these compounds were elucidated mainly based on the spectroscopic data, including 1D and 2D NMR, as well as high-resolution mass spectrometry. In particular, the amounts of obtained compounds 2 and 3 were only 200 μg and much less than 50 μg, respectively. Therefore, some carbons signals could not be observed on 13C NMR spectra of these compounds. However, the detailed analysis of HSQC and HMBC spectra allowed us to elucidate their structures. For NMR measurements of compound 3, it was found that using an 800 MHz NMR machine equipped with a cryogenic probe and acetic acid-d4 as a solvent is essential. Compounds (1–3) were N-3′-carbonylbutyl group-connected hypoxanthines.

A new oxylipin (1) was isolated from cyanobacteria collected at Tokyo Bay, Japan. The structure of 1 was elucidated based on spectroscopic data including 1D and 2D NMR, as well as high-resolution mass spectrometry. The structure of 1 was elucidated to be (S)-2-hydroxy-3-(((2R,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H-pyran-2-yl)oxy)propyl (E)-8-hydroperoxyhexadec-6-enoate.

A new oxylipin (1 ) was isolated from cyanobacteria collected at Tokyo Bay, Japan. The structure of 1 was elucidated based on spectroscopic data including 1D and 2D NMR, as well as high-resolution mass spectrometry. The structure of 1 was elucidated to be (S )-2-hydroxy-3-(((2R ,3R ,4S ,5R ,6R )-3,4,5-trihydroxy-6-(hydroxymethyl)tetrahydro-2H -pyran-2-yl)oxy)propyl (E )-8-hydroperoxyhexadec-6-enoate.