Explore the vast range of titles available.

\"Six best-selling and award-winning authors bring to life a breathtaking epic novel illuminating the hopes, desires, and destinies of princesses and peasants, harlots and wives, fanatics and philosophers - six unforgettable women whose paths cross during one of the most tumultuous and transformative events in history: the French Revolution. Ribbons of Scarlet is a timely story of the power of women to start a revolution - and change the world. In late 18th-century France, women do not have a place in politics. But as the tide of revolution rises, women from gilded salons to the streets of Paris decide otherwise - upending a world order that has long oppressed them. Blue-blooded Sophie de Grouchy believes in democracy, education, and equal rights for women and marries the only man in Paris who agrees. Emboldened to fight the injustices of King Louis XVI, Sophie aims to prove that an educated populace can govern itself - but one of her students, fruit-seller Louise Audu, is hungrier for bread and vengeance than learning. When the Bastille falls and Louise leads a women's march to Versailles, the monarchy is forced to bend, but not without a fight. The king's pious sister, Princess Elisabeth, takes a stand to defend her brother, spirit her family to safety, and restore the old order, even at the risk of her head. But when fanatics use the newspapers to twist the revolution's ideals into a new tyranny, even the women who toppled the monarchy are threatened by the guillotine. Putting her faith in the pen, brilliant political wife Manon Roland tries to write a way out of France's blood-soaked Reign of Terror while pike-bearing Pauline Leon and steely Charlotte Corday embrace violence as the only way to save the nation. With justice corrupted by revenge, all the women must make impossible choices to survive - unless unlikely heroine and courtesan's daughter Emilie de Sainte-Amaranthe can sway the man who controls France's fate: the fearsome Robespierre.\"--provided by publisher.

CONTENTS: Summary 737 I. Chilling and freezing: two different stresses requiring different solutions 738 II. Identification of a major cis‐element in the control of cold gene expression 739 III. The CBF transcription factors (TFs) and their regulation 739 IV. Events downstream of CBFs 740 V. A post‐genomic view on global transcript changes in response to low temperature 741 VI. The effect of light and circadian signals on cold gene expression 742 VII. Post‐transcriptional regulation 742 VIII. A receptor for cold? 742 IX. What are the characteristics of plant cell thermometer(s)? 744 X. Low‐temperature signalling downstream of perception 744 XI. Unresolved questions 747 Acknowledgements 748 References 748 SUMMARY: Plant species exhibit a range of tolerances to low temperatures, and these constitute a major determinant of their geographical distribution and use as crops. When tolerance is insufficient, either chilling or freezing injuries result. A variety of mechanisms are employed to evade the ravages of extreme or sub‐optimal temperatures. Many of these involve cold‐responsive gene expression and require that the drop in temperature is first sensed by the plant. Despite intensive research over the last 100 yr or longer, we still cannot easily answer the question of how plants sense low temperature. Over recent years, genomic and post‐genomic approaches have produced a wealth of information relating to the sequence of events leading from cold perception to appropriate and useful responses. However, there are also crucial and significant gaps in the pathways constructed from these data. We describe the literature pertaining to the current understanding of cold perception, signalling and regulation of low‐temperature‐responsive gene expression in higher plants, raising some of the key questions that still intrigue plant biologists today and that could be targets for future work. Our review focuses on the control of gene expression in the pathways leading from cold perception to chilling and freezing tolerance.

Many people with chronic stroke (PwCS) exhibit deficits in step width modulation, an important strategy for walking balance. A single exposure to swing leg perturbations can temporarily strengthen this modulation. The objective of this parallel, double-blinded, randomized controlled trial was to investigate whether repeated perturbations cause sustained increases in step modulation (NCT02964039; funded by the VA). 54 PwCS at the Medical University of South Carolina were randomly assigned to one of three intervention groups: Control (n = 18), with minimal forces; Assistive (n = 18), pushing the swing leg toward a mechanically appropriate location; Perturbing (n = 18), pushing the swing leg away from a mechanically appropriate location. All intervention groups included 24 training sessions over 12-weeks with up to 30-minutes of treadmill walking while interfaced with a novel force-field and a 12-week follow-up period, with five interspersed assessment sessions. Our primary outcome measure was paretic step width modulation, the partial correlation between step width and pelvis displacement (ρ SW ). Secondarily, we quantified swing and stance leg contributions to step modulation, clinical assessments of walking balance and confidence, and real-world falls. Outcomes were analyzed for participants who completed all assessment sessions (n = 44). Only the Perturbing group exhibited significant increases in paretic ρ SW , which were present after 4-weeks of training and sustained through follow-up (t = 2.42–3.17). These changes were due to improved control of paretic swing leg positioning. However, perturbation-induced changes in step modulation were not always significantly greater than those in the Control group, and clinical assessments were similar across intervention groups. Participants in the Perturbing group experienced a lower fall rate than those in the Control group (incidence rate ratio = 0.53), although our small sample size warrants caution. The present results indicate that perturbations can cause sustained modifications of targeted biomechanical characteristics of post-stroke gait, although such changes alone may be insufficient to change more complex clinical assessments.

Plants have adapted to tolerate and survive constantly changing environmental conditions by reprogramming gene expression The dynamics of the contribution of alternative splicing (AS) to stress responses are unknown. RNA-sequencing of a time-series of Arabidopsis thaliana plants exposed to cold determines the timing of significant AS changes. This shows a massive and rapid AS response with coincident waves of transcriptional and AS activity occurring in the first few hours of temperature reduction and further AS throughout the cold. In particular, hundreds of genes showed changes in expression due to rapidly occurring AS in response to cold (“early AS” genes); these included numerous novel cold-responsive transcription factors and splicing factors/RNA binding proteins regulated only by AS. The speed and sensitivity to small temperature changes of AS of some of these genes suggest that fine-tuning expression via AS pathways contributes to the thermo-plasticity of expression. Four early AS splicing regulatory genes have been shown previously to be required for freezing tolerance and acclimation; we provide evidence of a fifth gene, U2B”-LIKE. Such factors likely drive cascades of AS of downstream genes that, alongside transcription, modulate transcriptome reprogramming that together govern the physiological and survival responses of plants to low temperature.

The ethylene response factor (ERF) family in Arabidopsis thaliana comprises 122 members in 12 groups, yet the biological functions of the majority remain unknown. Of the group IX ERFs, the IXc subgroup has been studied the most, and includes ERF1, ERF14 and ORA59, which play roles in plant innate immunity. Here we investigate the biological functions of two members of the less studied IXb subgroup: ERF5 and ERF6. In order to identify potential targets of these transcription factors, microarray analyses were performed on plants constitutively expressing either ERF5 or ERF6. Expression of defense genes, JA/Et-responsive genes and genes containing the GCC box promoter motif were significantly upregulated in both ERF5 and ERF6 transgenic plants, suggesting that ERF5 and ERF6 may act as positive regulators of JA-mediated defense and potentially overlap in their function. Since defense against necrotrophic pathogens is generally mediated through JA/Et-signalling, resistance against the fungal necrotroph Botrytis cinerea was examined. Constitutive expression of ERF5 or ERF6 resulted in significantly increased resistance. Although no significant difference in susceptibility to B. cinerea was observed in either erf5 or erf6 mutants, the erf5 erf6 double mutant showed a significant increase in susceptibility, which was likely due to compromised JA-mediated gene expression, since JA-induced gene expression was reduced in the double mutant. Taken together these data suggest that ERF5 and ERF6 play positive but redundant roles in defense against B. cinerea. Since mutual antagonism between JA/Et and salicylic acid (SA) signalling is well known, the UV-C inducibility of an SA-inducible gene, PR-1, was examined. Reduced inducibilty in both ERF5 and ERF6 constitutive overexepressors was consistent with suppression of SA-mediated signalling, as was an increased susceptibility to avirulent Pseudomonas syringae. These data suggest that ERF5 and ERF6 may also play a role in the antagonistic crosstalk between the JA/Et and SA signalling pathways.

For more than 50 years, the United States (US) Endangered Species Act (ESA) has contributed to the protection, survival, and recovery of numerous species including gray wolves ( Canis lupus ), grizzly bears ( Ursus arctos horribilis ), whooping cranes ( Grus americana ), and spotted owls ( Strix occidentalis ). Recovery efforts by US federal agencies have largely focused on these well-known, charismatic species. As a result, lesser-known species have often been given lower priority and limited funding. An additional challenge to species recovery has been that ~ 50% of listed species have at least part of their habitats on private lands. Private landowners have sometimes viewed contributing to species recovery as an infringement on property rights. The Preble’s Meadow Jumping Mouse, Zapus hudsonius preblei , (PMJM) is a riparian specialist that serves as an indicator species for the ecological integrity of first and second-order watersheds in the Rocky Mountain region of the US. In 1998, PMJM was listed as a threatened species by the US Fish and Wildlife Service (USFWS). The listing process was prolonged by numerous lawsuits. After two different recovery teams and 21 years to produce a Recovery Plan, the USFWS recognized the need for a different approach to listed species recovery. The result, in 2019, was the establishment of the first Site Conservation Team (SCT) by the USFWS. This new SCT model uniquely combined: community-based participation and collaboration, private landowner leadership of process development and conservation action, extensive community outreach, a scientific foundation, and emphasized watershed health. The establishment of SCTs to address species conservation marked a new era taken by the USFWS. Herein we discuss the PMJM SCT located within the North Fork of the Cache la Poudre River watershed (North Fork SCT) in Northern Colorado. We identify conditions that enabled the new approach, describe the collaborative process, report how community engagement can address fears and lead to shared understanding of environmental benefits, and result in restoration actions that contribute to the species recovery. The North Fork SCT successfully nominated a recovery population nomination, developed a comprehensive conservation plan, and initiated restoration on 102 stream miles of potential habitat for approximately 4,000 PMJM individuals.

• Forward genetic screens play a key role in the identification of genes contributing to plant stress tolerance. Using a screen for freezing sensitivity, we have identified a novel freezing tolerance gene, SENSITIVE-TO-FREEZING8, in Arabidopsis thaliana. • We identified SFR8 using recombination-based mapping and whole-genome sequencing. As SFR8 was predicted to have an effect on cell wall composition, we used GC-MS and polyacrylamide gel electrophoresis to measure cell-wall fucose and boron (B)-dependent dimerization of the cell-wall pectic domain rhamnogalacturonan II (RGII) in planta. After treatments to promote borate-bridging of RGII, we assessed freeze-induced damage in wild-type and sfr8 plants by measuring electrolyte leakage from freeze-thawed leaf discs. • We mapped the sfr8 mutation to MUR1, a gene encoding the fucose biosynthetic enzyme GDP-D-mannose-4,6-dehydratase. sfr8 cell walls exhibited low cell-wall fucose levels and reduced RGII bridging. Freezing sensitivity of sfr8 mutants was ameliorated by B supplementation, which can restore RGII dimerization. B transport mutants with reduced RGII dimerization were also freezing-sensitive. • Our research identifies a role for the structure and composition of the plant primary cell wall in determining basal plant freezing tolerance and highlights the specific importance of fucosylation, most likely through its effect on the ability of RGII pectin to dimerize.

Many individuals who experience a stroke exhibit reduced modulation of their mediolateral foot placement, an important gait stabilization strategy. One factor that may contribute to this deficit is altered somatosensory processing, which can be probed by applying vibration to the involved muscles (e.g., the hip abductors). The purpose of this study was to investigate whether appropriately controlled hip abductor vibration can increase foot placement modulation among people with chronic stroke. 40 people with chronic stroke performed a series of treadmill walking trials without vibration and with vibration of either the hip abductors or lateral trunk (a control condition) that scaled with their real-time mediolateral motion. To assess participants’ vibration sensitivity, we also measured vibration detection threshold and lateral sway evoked by abductor vibration during quiet standing. As a group, foot placement modulation increased significantly with either hip or trunk vibration, compared to without vibration. However, these changes were quite variable across participants, and were not predicted by either vibration detection threshold or the lateral sway evoked by hip vibration during standing. Overall, we found that somatosensory stimulation had small, positive effects on post-stroke foot placement modulation. Unexpectedly, these effects were observed with both hip abductor and lateral trunk vibration, perhaps indicating that the trunk can also provide useful somatosensory feedback during walking. Future work is needed to determine whether repeated application of such somatosensory stimulation can produce sustained effects on this important gait stabilization strategy.

The Mediator16 (MED16; formerly termed SENSITIVE TO FREEZING6 [SFR6]) subunit of the plant Mediator transcriptional coactivator complex regulates cold-responsive gene expression in Arabidopsis thaliana, acting downstream of the C-repeat binding factor (CBF) transcription factors to recruit the core Mediator complex to cold-regulated genes. Here, we use loss-of-function mutants to show that RNA polymerase II recruitment to CBF-responsive cold-regulated genes requires MED16, MED2, and MED14 subunits. Transcription of genes known to be regulated via CBFs binding to the C-repeat motif/drought-responsive element promoter motif requires all three Mediator subunits, as does cold acclimation-induced freezing tolerance. In addition, these three subunits are required for low temperature-induced expression of some other, but not all, cold-responsive genes, including genes that are not known targets of CBFs. Genes inducible by darkness also required MED16 but required a different combination of Mediator subunits for their expression than the genes induced by cold. Together, our data illustrate that plants control transcription of specific genes through the action of subsets of Mediator subunits; the specific combination defined by the nature of the stimulus but also by the identity of the gene induced.

Diligent side-by-side comparisons of how different methodologies affect growth efficiency and quality of intestinal colonoids have not been performed leaving a gap in our current knowledge. Here, we summarize our efforts to optimize culture conditions for improved growth and functional differentiation of mouse and human colon organoids. Mouse and human colon organoids were grown in four different media. Media-dependent long-term growth was measured by quantifying surviving organoids imaging and a cell viability readout over five passages. The impact of diverse media on differentiation was assessed by quantifying the number of epithelial cell types using markers for enterocytes, stem cells, Goblet cells, and enteroendocrine cells by qPCR and histology upon removal of growth factors. In contrast to Wnt3a-conditioned media, media supplemented with recombinant Wnt3a alone did not support long-term survival of human or mouse colon organoids. Mechanistically, this observation can be attributed to the fact that recombinant Wnt3a did not support stem cell survival or proliferation as demonstrated by decreased LGR5 and Ki67 expression. When monitoring expression of markers for epithelial cell types, the highest level of organoid differentiation was observed after combined removal of Wnt3a, Noggin, and R-spondin from Wnta3a-conditioned media cultures. Our study defined Wnt3a-containing conditioned media as optimal for growth and survival of human and mouse organoids. Furthermore, we established that the combined removal of Wnt3a, Noggin, and R-spondin results in optimal differentiation. This study provides a step forward in optimizing conditions for intestinal organoid growth to improve standardization and reproducibility of this model platform.