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Effective in-season weed management options are limited for organic cereal farmers. Two alternatives to current farmer practices are improving efficacy of physical weed control through use of interrow cultivation or increasing the competitive ability of the crop through elevated seeding rates and more uniform spatial planting patterns. It is unknown how these two methods affect yield, quality, and economic returns. Field experiments were conducted in the northeast United States to determine whether the yield gain from increased weed control from these contrasting weed management strategies resulted in increased net returns and how these different systems affected grain quality. Wheat was planted at two seeding rates (400 and 600 plants m−2), in three row spacings (11, 18, and 23 cm). A fourth crop arrangement that approaches a more uniform spatial distribution through a combination of drilling and broadcasting seed was included. For weed control, treatments received tine harrowing. Wheat sown in wide rows also received interrow cultivation. Each system was sown in the presence and absence of condiment mustard, which was sown as a surrogate weed. Increased seeding rate reduced weed density 64% compared to a crop-free check and 30% compared to regional farmers' practices of 18-cm rows and 400 plants m−2. Increased seeding rates lowered grain protein 5% compared to standard seeding rates. Wide rows, in combination with interrow cultivation, reduced weed density 62%, increased yield 16%, and net returns 19% compared to regional organic practices. Significant increases in grain N were limited to weed-free plots. While increased seeding rates improved weed suppression, the high input cost of organic seed make this an unsatisfactory alternative to interrow cultivation and current farmer practices, as yield would need to be .15 t ha−1 higher at elevated density to offset the extra cost of seed. Nomenclature: Wheat, Triticum aestivum L.; white mustard, Sinapis alba L.

Gastroenterologists at all levels of practice benefit from formal mentoring. Much of the current literature on mentoring in gastroenterology is based on expert opinion rather than data. In this study, we aimed to identify gender-related barriers to successful mentoring relationships from the mentor and mentee perspectives. A voluntary, web-based survey was distributed to physicians at 20 academic institutions across the United States. Overall, 796 gastroenterology fellows and faculty received the survey link, with 334 physicians responding to the survey (42% response rate), of whom 299 (90%; 129 women and 170 men) completed mentorship questions and were included in analysis. Responses of women and men were compared. Compared with men, more women preferred a mentor of the same gender (38.6% women vs 4.2% men, P < 0.0001) but less often had one (45.5% vs 70.2%, P < 0.0001). Women also reported having more difficulty finding a mentor (44.4% vs 16.0%, P < 0.0001) and more often cited inability to identify a mentor of the same gender as a contributing factor (12.8% vs 0.9%, P = 0.0004). More women mentors felt comfortable advising women mentees about work-life balance (88.3% vs 63.8%, P = 0.0005). Nonetheless, fewer women considered themselves effective mentors (33.3% vs 52.6%, P = 0.03). More women reported feeling pressured to mentor because of their gender (39.5% vs 0.9% of men, P < 0.0001). Despite no gender differences, one-third of respondents reported negative impact of the COVID-19 pandemic on their ability to mentor and be mentored. Inequities exist in the experiences of women mentees and mentors in gastroenterology, which may affect career advancement and job satisfaction.

INTRODUCTION:Gastroenterologists at all levels of practice benefit from formal mentoring. Much of the current literature on mentoring in gastroenterology is based on expert opinion rather than data. In this study, we aimed to identify gender-related barriers to successful mentoring relationships from the mentor and mentee perspectives.METHODS:A voluntary, web-based survey was distributed to physicians at 20 academic institutions across the United States. Overall, 796 gastroenterology fellows and faculty received the survey link, with 334 physicians responding to the survey (42% response rate), of whom 299 (90%; 129 women and 170 men) completed mentorship questions and were included in analysis.RESULTS:Responses of women and men were compared. Compared with men, more women preferred a mentor of the same gender (38.6% women vs 4.2% men, P < 0.0001) but less often had one (45.5% vs 70.2%, P < 0.0001). Women also reported having more difficulty finding a mentor (44.4% vs 16.0%, P < 0.0001) and more often cited inability to identify a mentor of the same gender as a contributing factor (12.8% vs 0.9%, P = 0.0004). More women mentors felt comfortable advising women mentees about work-life balance (88.3% vs 63.8%, P = 0.0005). Nonetheless, fewer women considered themselves effective mentors (33.3% vs 52.6%, P = 0.03). More women reported feeling pressured to mentor because of their gender (39.5% vs 0.9% of men, P < 0.0001). Despite no gender differences, one-third of respondents reported negative impact of the COVID-19 pandemic on their ability to mentor and be mentored.DISCUSSION:Inequities exist in the experiences of women mentees and mentors in gastroenterology, which may affect career advancement and job satisfaction.

A key mechanism of antiviral antibodies is to bind cell-surface viral antigens and activate cellular immunity to clear infected cells, yet antibodies targeting human cytomegalovirus (HCMV) have exhibited limited efficacy. This appears due to HCMV's multiple immune evasion mechanisms, including viral receptors (vFcγRs) which bind human IgG Fc domains to co-operatively inhibit Fc activation of host Fcγ receptors and impair Fc-mediated effector functions. We biochemically characterized and evaluated the functions of two highly conserved vFcγRs, gp34 and gp68, and mapped their binding epitopes on the Fc domain. Based on this information, we then engineered Fc variants that retain binding to CD16A, which is essential for NK activation, and to FcRn but have markedly attenuated binding to gp34 and gp68. IgG1 antibodies targeting the gB fusogen with engineered Fc domains were not internalized by infected cells, mediated enhanced CD16A activation and limited viral spread in HCMV-infected fibroblasts more effectively than wild-type Fc. Together, this work demonstrates a strategy to enhance the efficacy of antibody therapies to clear HCMV infections. Host and HCMV FcR compete for IgG1 binding but engage different residues.Fc-engineering abrogates viral FcR antagonism while retaining CD16A activation.Antibodies that resist vFcR capture promote superior ADCC against infected cells.Designer Fc domains complement Fabs to create enhanced disease-specific therapies.

A key mechanism of antiviral antibodies is to bind cell-surface viral antigens and activate cellular immunity to clear infected cells, yet antibodies targeting human cytomegalovirus (HCMV) have exhibited limited efficacy. This appears due to its multiple immune evasion mechanisms, including viral receptors (vFcgRs) which bind human IgG Fc domains to co-operatively inhibit Fc activation of host Fcg receptors and impair Fc-mediated effector functions. We biochemically characterized and evaluated the functions of two highly conserved vFcgRs, gp34 and gp68, and mapped their binding epitopes on the Fc domain. Based on this information, we then engineered Fc variants that retain binding to CD16A, which is essential for NK activation, and to FcRn but have markedly attenuated binding to gp34 and gp68. IgG1 antibodies targeting the gB fusogen with engineered Fc domains were not internalized by infected cells, mediated enhanced CD16A activation and limited viral spread in HCMV-infected fibroblasts more effectively than wild-type Fc. Together, this work demonstrates a strategy to enhance the efficacy of antibody therapies to clear HCMV infections.