Explore the vast range of titles available.

Osteosarcoma is the predominant primary malignant bone tumor that poses a significant global health challenge. MicroRNAs (miRNAs) that regulate gene expression are associated with osteosarcoma pathogenesis. Thus, miRNAs are potential therapeutic targets for osteosarcoma. Nanoparticles, widely used for targeted drug delivery, facilitate miRNA-based osteosarcoma treatment. Numerous studies have focused on miRNA delivery using nanoparticles to inhibit the progress of osteosarcoma. Polymer-based, lipid-based, inorganic-based nanoparticles and extracellular vesicles were used to deliver miRNAs for the treatment of osteosarcoma. They can be modified to enhance drug loading and delivery capabilities. Also, miRNA delivery was combined with traditional therapies, for example chemotherapy, to treat osteosarcoma. Consequently, miRNA delivery offers promising therapeutic avenues for osteosarcoma, providing renewed hope for patients. This review emphasizes the studies utilizing nanoparticles for miRNA delivery in osteosarcoma treatment, then introduced and summarized the nanoparticles in detail. And it also discusses the prospects for clinical applications.

Diabetic foot ulcers often become infected, leading to treatment complications and increased risk of loss of limb. Therapeutics to manage infection and simultaneously promote healing are needed. Here we report on the development of a Janus liposozyme that treats infections and promotes wound closure and re-epithelialization. The Janus liposozyme consists of liposome-like selenoenzymes for reactive oxygen species (ROS) scavenging to restore tissue redox and immune homeostasis. The liposozymes are used to encapsulate photosensitizers for photodynamic therapy of infections. We demonstrate application in methicillin-resistant Staphylococcus aureus -infected diabetic wounds showing high ROS levels for antibacterial function from the photosensitizer and nanozyme ROS scavenging from the liposozyme to restore redox and immune homeostasis. We demonstrate that the liposozyme can directly regulate macrophage polarization and induce a pro-regenerative response. By employing single-cell RNA sequencing, T cell-deficient Rag1 −/− mice and skin-infiltrated immune cell analysis, we further reveal that IL-17-producing γδ T cells are critical for mediating M1/M2 macrophage transition. Manipulating the local immune homeostasis using the liposozyme is shown to be effective for skin wound repair and tissue regeneration in mice and mini pigs. Therapies for treating bacterial infection and increasing wound healing are needed. Here the authors report a liposozyme that combines reactive oxygen species generation and scavenging for antibacterial action and modulation of redox and immune homeostasis, increasing wound healing.

Osteoarthritis (OA) is a common chronic degenerative condition in the elderly, in which inflammation plays a key role in disease pathology. Lycopene (Lye), a member of the carotenoid family, has been reported to have anti‐inflammatory effects. The purpose of this study was to investigate the effect of Lye on the inflammation of chondrocytes and the mouse OA model. Chondrocytes were treated with interleukin (IL)‐1β, and the mouse OA model was induced by the surgical destabilization of the medial meniscus (DMM). The results showed that Lye could inhibit the expression of inflammatory factors and alleviate the degradation of extracellular matrix (ECM). Additionally, Lye could activate the Nrf2/HO‐1 pathway and reverse the activations of NF‐κB and STAT3 signal pathway induced by IL‐1β, suggesting that its anti‐inflammatory effect may be mediated via these pathways. The animal experiments showed that Lye could decrease the Osteoarthritis Research Society International (OARSI) scores of the knee, indicating that it could inhibit the occurrence and development of OA in mouse. Overall, our results indicated that Lye might be used as a novel drug for OA treatment.

Amino acids are naturally occurring compounds in many edible or medicinal plants, which possess a variety of nutraceutical effects in human. Here, a method of ultrasound-assisted extraction was optimized using Box–Behnken design of response surface methodology in maximizing the yield of free amino acids deriving from flowers of Apocynum venetum L. Under the optimal condition of ultrasound-assisted extraction, i.e. 187.5 W of ultrasonic power, 31.93 min of extraction time, and 0.47 mg/ml of solid–liquid ratio, the experimental yield of extractive was 287.17 ± 0.205 mg/g, which was in close agreement with the value, as predicted by the established model. In addition, a hydrophilic interaction ultra-high performance liquid chromatography coupled with QqQ-MS/MS method was developed and validated for simultaneous quantification of 20 types of amino acids without derivatization contained in A. venetum flowers. The analytical method was validated by matrix effect, linearity, limit of detection, limit of quantification, precision, repeatability, stability, and recovery. The analysis results showed that A. venetum flower was rich in free amino acids of ~3% of total dried weight, and which contained leucine (13.7 g/mg), isoleucine (7.9 g/mg), and lysine (2.2 g/mg) as the most abundant amino acids. Thus, A. venetum flower could provide beneficial nutrient values for human health, e.g. adult weight maintenance or glucose homeostasis.