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Acrylamide (ACR) with its extensive industrial applications is a classified occupational hazard toxin and carcinogenic compound. Its formation in fried potatoes, red meat and coffee during high-temperature cooking is a cause for consideration. The fabrication of chitosan-coated probiotic nanoparticles (CSP NPs) aims to enhance the bioavailability of probiotics in the gut, thereby improving their efficacy against ACR-induced toxicity in Drosophila melanogaster . Nanoencapsulation, a vital domain of the medical nanotechnology field plays a key role in targeted drug delivery, bioavailability, multi-drug load delivery systems and synergistic treatment options. Our study exploited the nanoencapsulation technology to coat Lactobacillus fermentum (probiotic) with chitosan (prebiotic), both with substantial immunomodulatory effects, to ensure the stability and sustained release of microbial load and its secondary metabolites in the gut. The combination of pre-and probiotic components, called synbiotic formulations establishes the correlation between the gut microbiota and the overall well-being of an organism. Our study aimed to develop a potent synbiotic to alleviate the impacts of heat-processed dietary toxins that significantly influence behaviour, development, and survival. Our synbiotic co-treatment with ACR in fruit flies normalised neuro-behavioural, survival, redox status, and restored ovarian mitochondrial activity, contrasting with several physiological deficits observed in the ACR-treated model.

Acrylamide (ACR), a neurotoxin typically present in thermally processed foods, is a substantial risk to people. The objective of this research is to develop synbiotic capsules with natural substances such as chitosan, alginate, and L. fermentum . Encapsulation is a significant tool in medicine, helping to improve targeted medication delivery and bioavailability. The chitosan/alginate-encapsulated probiotic (CAP) beads increase the bioavailability of probiotics in the gut, allowing for a more effective response to ACR-induced toxicity. The combination of prebiotic and probiotic activity improves stability, viability, and gastrointestinal delivery. We developed CAP beads and assessed their survivability under simulated gastrointestinal conditions, encapsulating efficiency, and release profile. The efficacy of these beads in reducing the harmful effects of ACR was subsequently investigated using a Drosophila melanogaster model. Under co-exposure and pre-treatment settings, in vivo studies revealed restoration of locomotor activities, redox balance, and ovarian mitochondrial membrane potential in flies treated with CAP beads. Furthermore, implying the indirect impact of CAP beads on gut microbiota and xenobiotic metabolism, pre-treatment with CAP more successfully restored the expression of important antioxidant and stress-related genes, including sod , cat , InR , rpr , and p53 .

Background Self-management is an essential component of prevention and treatment of type 2 diabetes. Social and family support has been shown to influence self-management behaviors as well as glycemic control and complications. This study was conducted to assess whether diabetes family support improves diabetes self-management and glycemic control in a typical urban population in India. Methods A cross-sectional study using a questionnaire that had items from the Summary of Diabetes Self Care Activities Scale (SDSCA), the Diabetes Family Behavior Checklist (DFBC) and some sociodemographic and diabetes related clinical data was conducted. The participants were consecutively sampled from the diabetes outpatient department in a tertiary care hospital in Chennai, south India. Results A total of 200 consecutive patients from the diabetes outpatient department were interviewed. Diabetes self-management practices were good with respect to avoiding fatty foods and carbohydrates and undergoing regular blood testing for glucose. But the self-management with respect to exercise and foot related care was rare. It was observed that a vast majority of the patients did not report receiving any support from their families. However, in the small proportion who did receive good family support, there is an association between diabetes self-management and diabetes family support (β = 0.527; p  = 0.015). Further, the path model showed that there is a positive statistically significant association between family support score and the diabetes self-management score (β = 0.254, p  < 0.001). However, the negative association between the diabetes self-management score and the mean plasma glucose did not reach statistical significance (β = − 46.378, p  = 0.082). Conclusions In the urban south Indian setting, family support was significantly associated with better self-management activities, but better self-management did not reflect as better glycaemic control.

Convalescent plasma is a leading treatment for coronavirus disease 2019 (COVID-19), but there is a paucity of data identifying its therapeutic efficacy. Among 126 potential convalescent plasma donors, the humoral immune response was evaluated using a severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus neutralization assay with Vero-E6-TMPRSS2 cells; a commercial IgG and IgA ELISA to detect the spike (S) protein S1 domain (EUROIMMUN); IgA, IgG, and IgM indirect ELISAs to detect the full-length S protein or S receptor-binding domain (S-RBD); and an IgG avidity assay. We used multiple linear regression and predictive models to assess the correlations between antibody responses and demographic and clinical characteristics. IgG titers were greater than either IgM or IgA titers for S1, full-length S, and S-RBD in the overall population. Of the 126 plasma samples, 101 (80%) had detectable neutralizing antibody (nAb) titers. Using nAb titers as the reference, the IgG ELISAs confirmed 95%-98% of the nAb-positive samples, but 20%-32% of the nAb-negative samples were still IgG ELISA positive. Male sex, older age, and hospitalization for COVID-19 were associated with increased antibody responses across the serological assays. There was substantial heterogeneity in the antibody response among potential convalescent plasma donors, but sex, age, and hospitalization emerged as factors that can be used to identify individuals with a high likelihood of having strong antiviral antibody responses.

Remote sensing has been heralded as the silver bullet in water quality modeling and watershed management, and yet a quantitative mapping of where its applicability is likely and most useful has not been undertaken so far. Here, we combine geospatial models of cloud cover as a proxy for the likelihood of acquiring remote scenes and the shortest time of travel to population centers as a proxy for accessibility to ground-truth remote sensing data for water quality monitoring and produce maps of the potential of remote sensing in watershed management in the United States. We generate several maps with different cost-payoff relationships to help stakeholders plan and incentivize remote sensing-based monitoring campaigns. Additionally, we combine these remote sensing potential maps with spatial indices of population, water demand, ecosystem services, pollution risk, and monitoring coverage deficits to identify where remote sensing likely has the greatest role to play. We find that the Southwestern United States and the Central plains regions are generally suitable for remote sensing for watershed management even under the most stringent costing projections, but that the potential for using remote sensing can extend further North and East as constraints are relaxed. We also find large areas in the Southern United States and sporadic watersheds in the Northeast and Northwest seaboards and the Midwest would likely benefit most from using remote sensing for watershed monitoring. Although developed herein for watershed decision support in the United States, our approach is readily generalizable to other environmental domains and across the world.

Background Obesity, a growing problem worldwide and locally, is a risk factor for many chronic conditions. Weight loss has been shown to improve health outcomes and weight management programmes are effective in achieving weight loss. However, the effectiveness and sustainability of weight management programmes are variable. The six-month, seven-touch-point Weight Management Programme (WeightWise) is a locally developed multidisciplinary programme based on the latest evidence and expert opinion. This study aimed to explore the perceived barriers and facilitators to delivering WeightWise prior to its implementation in the Singapore public primary care setting (polyclinics). Methods Between September 2023 and January 2024, a qualitative study was conducted across two polyclinics designated to pilot WeightWise. Twenty-six healthcare practitioners, including clinic directors, doctors, nurses, dietitians and physiotherapists, who would be involved in running WeightWise or referring patients to WeightWise, were interviewed by the researchers using an interview guide, in either a focus group or individually. Interviews were audio-recorded, transcribed verbatim, checked and analysed thematically using the NVivo software. Results The participants in this study expressed a gap in weight management in primary care and found WeightWise relevant and timely. However, they highlighted potential barriers to implementing WeightWise in terms of patient enrolment and programme delivery. For enrolment to WeightWise, the participants were concerned about a lack of prioritisation by the practitioners on weight management, time constraint, complex referral criteria and pricing. However, they highlighted potential facilitators to enrolment including targeting young and motivated patients, as well as identifying eligible patients at pre-consult vitals measurement stations. For WeightWise delivery, the perceived barriers were: increased workload for nurses, difficulty in accessing information on community exercise programmes, lack of weight management knowledge amongst practitioners, inconvenient programme timings and uncertainty in long-term benefits of WeightWise. The perceived facilitators to delivering WeightWise were: the use of remote care modalities, preventive care awareness through Healthier SG (a national health initiative), training programmes for practitioners and continuous peer support for patients. Conclusion This study identified the need to focus on both enrolment and delivery when implementing WeightWise. It also identified low prioritisation of weight management, lack of practitioner knowledge, lack of resources and costs as key barriers when implementing a weight management programme. Tailored healthcare professionals training, additional resources and cost analysis is recommended before its implementation. Clinical trial number Not applicable.

In order to evaluate the impact of plant-based hydrolysates on CHO cells, a transcriptomic study was undertaken using cottonseed hydrolysate and Illumina’s NextSeq transcriptomics profiling for 2 days of a batch cell culture. While cottonseed hydrolysate extended cell growth and increased antibody titer, significant effects were seen on transcriptomic signatures of supplemented cultures when compared to untreated cultures, evaluated using fold change, gene ontology (GO), and KEGG pathway analysis. Transcription and other factors commonly associated with cell growth such as those of the Atf family and homeobox proteins were upregulated while genes in the Hippo signaling pathway were downregulated. Genes involved in anabolic pathways such as gluconeogenesis and those involving protein folding and translation elongation were upregulated. GO analysis of biological processes for cottonseed-supplemented cultures indicated enrichments in DNA replication, protein processing, and unfolded protein response while molecular functions associated with growth such as GTPases, ATP binding, and aminoacyl t-RNA ligase activity were also enriched. Cellular components associated with structural integrity such as actin cytoskeleton, microtubules, mitochondrion, and Lewy body were enriched. Enriched KEGG pathways include growth-associated pathways such as cell cycle, pI3K-AKT-mTOR, and cancer-related pathways as well as those enhancing glycan metabolism, purine metabolism, amino acid biosynthesis, and protein processing in the endoplasmic reticulum (ER). These transcriptomic profiles provide insights into the roles that hydrolysates such as cottonseed can play in altering CHO cell growth and other physiological characteristics as well as suggesting ways in which CHO cell culture may be modified for enhancing performance in biotechnology applications.Key points• Hydrolysate-supplemented cultures increased mammalian cell growth and productivity.• Fold-change analysis revealed upregulation in transcription and translation.• Enriched GOs and KEGG pathways including cell cycle and metabolism were observed.

The current global scenario has instigated a steady upsurge of synthetic chemicals usage thereby creating a toxic environment unsuitable for animals and humans. Acrylamide and bisphenol A are some of the most common toxins found in the atmosphere due to their extensive involvement in numerous industrial processes. Acrylamide, an occupational hazard toxin has been known to cause severe nerve damage and peripheral neuronal damage in both animals and humans. General sources of acrylamide exposure are effluents from textile and paper industries, cosmetics, and thermally processed foods rich in starch. Bisphenol A (BPA) is generally found in food packaging materials, dental sealants, and plastic bottles. It is highly temperature-sensitive that can easily leach into the food products or humans on contact. The genotoxic and neurotoxic effects of acrylamide and bisphenol A have been widely researched; however, more attention should be dedicated to understanding the developmental toxicity of these chemicals. The developmental impacts of toxin exposure can be easily understood using Drosophila melanogaster as a model given considering its short life span and genetic homology to humans. In this review, we have discussed the toxic effects of acrylamide and BPA on the developmental process of Drosophila melanogaster .

Background. The optimum time of labour induction among women with a previous caesarean without any pregnancy complication and eligible and willing for vaginal delivery is not specified. This study compares the vaginal birth rates between induction at 40 weeks and expectant management till 41 weeks. Method. We conducted this parallel design nonblinded, randomized controlled trial in a tertiary care teaching institution in South India on women with a previous lower segment caesarean section eligible for a trial of labour with singleton foetus without any pregnancy complication at recruitment. We screened 1886 women. Sixty women underwent block (of 6 each) randomization into two groups of thirty each at 40 weeks. We induced the women in the intervention group at 40 weeks with oxytocin or a single 24-hour application of a Foley catheter followed by oxytocin infusion and amniotomy. The expectant group underwent maternal and foetal surveillance and induction at 41 weeks with the same protocol if not delivered by then. We compared the primary outcome of the proportion of vaginal birth rate with a chi-square test. Result. Data from all sixty women were analyzed. Twenty (66.67%) in the induction compared to ten (33.33%) in the expectant group delivered vaginally. This difference was significant (RR 2.0, 95% CI: 1.13-3.52; P=0.016). One woman in the expectant group had scar dehiscence. Conclusion. Among women with a previous caesarean scar, labour induction at 40 weeks has a significantly higher vaginal birth rate than those managed expectantly till 41 weeks. More extensive trials are feasible and recommended. Trial Registry. The trial was prospectively registered with the clinical trial registry of India. This trial is registered with CTRI/2018/09/015719 (date of registration 14th September 2018).

Background: The role of primary care nurses is expanding significantly due to increasing healthcare demand. This role expansion poses various challenges to the nurses that can impact patient care. Using hypertension care as an example, this study aimed to explore the diverse roles played by primary care nurses and the challenges they faced when performing these roles. Methods: An exploratory qualitative study was conducted among nurses in 5 public primary care clinics in Singapore. Purposive sampling was employed to ensure nurses of different cadre and experiences were included. An experienced qualitative researcher conducted the interviews until data saturation was reached. The interviews were audio-recorded, transcribed verbatim, checked and analysed thematically using NVivo (version 14). Results: Thirteen nurses of varying age (25-65 years) and experience level (staff nurse to advanced practice nurse) were interviewed. The nurses’ traditional duties in primary care settings included patient triaging, immunisation administration, management of acute and chronic conditions including regular health monitoring, wound care, supporting medication adherence, general lifestyle counselling and mentoring junior nurses. However, they performed expanded clinical roles for hypertension care with additional responsibilities that included counselling on home-BP monitoring and medication titration using motivational interviewing. The key challenges they faced in managing hypertension were: (i) time and manpower constraint, (ii) lack of continuity of care, (iii) lack of clear guidelines, (iv) inefficient workflows, (v) overlapping and competing services, (vi) lack of knowledge and training and (vii) lack of home BP readings from patients. Conclusion: This study contributes to the ongoing conversation on the future of nursing, highlighting the importance of balancing professionalism with the increasing demands of healthcare. Our results emphasise the need for strategic planning and role clarification through clear communication of job descriptions, routine reviewing of clinical workflows, targeted training and support for nurses in performing their diverse roles, and fostering regular multidisciplinary meetings to define, communicate and support evolving nursing responsibilities in primary care.