Explore the vast range of titles available.

Background Helicobacter pylori , the main cause of various gastric diseases, infects approximately half of the human population. This pathogen is auxotrophic for cholesterol which it converts to various cholesteryl α-glucoside derivatives, including cholesteryl 6’-acyl α-glucoside (CAG). Since the related biosynthetic enzymes can be translocated to the host cells, the acyl chain of CAG likely comes from its precursor phosphatidylethanolamine (PE) in the host membranes. This work aims at examining how the acyl chain of CAG and PE inhibits the membrane functions, especially bacterial adhesion. Methods Eleven CAGs that differ in acyl chains were used to study the membrane properties of human gastric adenocarcinoma cells (AGS cells), including lipid rafts clustering (monitored by immunofluorescence with confocal microscopy) and lateral membrane fluidity (by the fluorescence recovery after photobleaching). Cell-based and mouse models were employed to study the degree of bacterial adhesion, the analyses of which were conducted by using flow cytometry and immunofluorescence staining, respectively. The lipidomes of H. pylori, AGS cells and H. pylori –AGS co-cultures were analyzed by Ultraperformance Liquid Chromatography-Tandem Mass Spectroscopy (UPLC-MS/MS) to examine the effect of PE(10:0) 2 , PE(18:0) 2 , PE(18:3) 2 , or PE(22:6) 2 treatments. Results CAG10:0, CAG18:3 and CAG22:6 were found to cause the most adverse effect on the bacterial adhesion. Further LC–MS analysis indicated that the treatment of PE(10:0) 2 resulted in dual effects to inhibit the bacterial adhesion, including the generation of CAG10:0 and significant changes in the membrane compositions. The initial (1 h) lipidome changes involved in the incorporation of 10:0 acyl chains into dihydro- and phytosphingosine derivatives and ceramides. In contrast, after 16 h, glycerophospholipids displayed obvious increase in their very long chain fatty acids, monounsaturated and polyunsaturated fatty acids that are considered to enhance membrane fluidity. Conclusions The PE(10:0) 2 treatment significantly reduced bacterial adhesion in both AGS cells and mouse models. Our approach of membrane remodeling has thus shown great promise as a new anti- H. pylori therapy. Graphical Abstract

Face-swap apps bring various benefits and incur risks for app users. The face swap apps are enjoyful for most users but the disclosure of personal information when using these apps is associated with an increase in threats such as identity theft, personal information leaks, and fraud. This research aims to integrate the Technology Acceptance Model (TAM) and the Protection Motivation Theory (PMT) to examine the influence of face swap app benefits and Perceived risks on face swap app usage intention. The research is conducted through an online/offline survey of 289 participants from different demographics in Vietnam. Structural equation modeling is applied to investigate the relationships between the variables. This study recognizes the negative impact of potential deepfake risks associated with the usage of face-swap applications. The results demonstrate that elevated Privacy Risk, Information Manipulation and Security Risk Perceptions result in a decreased intention to use face-swapping applications (standardized β = −.135; −.125; −.163, respectively), whereas Entertainment level, Ease of use, Social Influence substantially encourage user engagement with these applications (standardized β = .305; .280; .248, respectively). This study employs a comprehensive theoretical model that combine PMT and TAM to investigate the effects of perceived benefits and risk dimensions on face-swap apps usage intention. This study has practical implications for AI app and social media developers by offering deeper insights into the factors that attract or discourage face-swap users.

Never human being has to face such a serious lack of phosphorus and pollutants from human activities. Nutrient recovery from wastewater is a new trend which attracts the interests of several researchers. Extraction of the nutrients, based on struvite crystal from wastewater as nutritious sources, has been assessed as an urgent solution to tackle the water pollution issue. This review focused on feature characteristics of struvite as a chemical fertilizer for plant, struvite formation process in various wastewaters, which is related to physio-chemical conditions, and potential of applying this idea into practice.

Background/purpose. Preschool education in Vietnam plays a critical role in fostering the development of children aged 3 months to 6 years. Enhancing teacher quality is essential to achieving educational goals, yet early childhood educators face significant challenges in professional skill development. This study examines the implementation of teacher development activities in the Mekong Delta region. Materials/methods. A mixed-methods approach was employed, collecting quantitative data from 552 participants (50 administrators and 502 teachers) and qualitative insights through open-ended questions. Results. Most institutions have structured plans for teacher development, with 81.2% assessing workforce needs and 91.8% establishing clear goals. Training participation is high, with 84.1% engaged in professional title courses and 73.9% in child-centered program development. Teachers favor courses on new programs, STEAM, and life skills education. Performance evaluations were generally positive, though teachers rated themselves higher (M = 4.09) than management (M = 3.36). Facility availability varied, with discrepancies between teacher and management perceptions. Policies remain inadequate, with only 10% finding salary support sufficient, underscoring the need for improved compensation. Conclusion. Strengthening teacher capacity, refining evaluation methods, and improving salary policies are key to advancing early childhood education. Addressing these areas can drive continuous improvement, transform teaching quality, and enhance educational outcomes in Vietnam.

Background Our study addresses the pressing need for safe and effective treatments for pneumonia in young children caused by respiratory syncytial virus (RSV) and bacterial co-infections. This issue is particularly urgent given the absence of targeted RSV therapies and the growing threat of antibiotic resistance associated with managing bacterial co-infections. Methods We conducted a double-blind, randomized clinical trial (ClinicalTrials.gov: NCT05929599) at Vietnam National Children’s Hospital to evaluate nasal-spraying Bacillus spore probiotics (LiveSpo Navax containing B. subtilis ANA4 and B. clausii ANA39 at ≥1 billion CFU/mL) in children aged 1-24 months with pneumonia due to RSV and bacterial co-infection. Participants were randomly assigned in a 1:1 ratio to receive standard care plus either LiveSpo Navax or physiological saline solution, using a simple sealed-number draw at enrollment. Primary outcomes were the median duration required to resolve common pneumonia symptoms, duration of oxygen therapy, and total treatment days. Results A total of 120 children are enrolled (60 per group). In the final analysis, 50 participants in the Control group and 51 in the Navax group are included. The trial is completed with no serious adverse events or treatment-related side effects in either group. Navax treatment shortens the duration of eight symptoms associated with RSV pneumonia by one day, oxygen therapy by two days, and overall treatment by one day. Conclusions The nasal-spraying Bacillus spore approach presents a safe, effective, and fast treatment for young children with pneumonia due to RSV and bacterial co-infections, making it especially a promising strategy for resource-limited settings. Plain language summary Pneumonia is a common lung infection caused by both respiratory syncytial virus (RSV) and harmful bacteria. While antibiotics are generally effective in treating the infection, their overuse has contributed to growing antibiotic resistance. In this study, we show that LiveSpo Navax-a probiotic spray containing billions of Bacillus spores (beneficial bacteria that can survive tough conditions)-is safe and helpful for children with pneumonia. Spraying LiveSpo Navax directly into the noses of children helped reduce their symptoms, RSV and bacterial infections, as well as the need for antibiotics. This affordable, simple, and fast treatment offers a promising way to support recovery from pneumonia, especially in areas with limited access to healthcare. Le, Phung, Bui et al. investigate nasal-spraying Bacillus spore probiotics (LiveSpo Navax) as a supportive treatment for Respiratory Syncytial Virus pneumonia with bacterial co-infections in young children. Use of the probiotic reduces symptoms, antibiotic use, viral and bacterial loads, and improves immune and nasal microbiota.

Background Breast cancer (BC) remains the second leading cause of cancer-related mortality among women worldwide. Liquid biopsy based on circulating tumor DNA (ctDNA) offers a promising noninvasive approach for early detection; however, differentiating malignant tumors from benign abnormalities remains a significant challenge. Results Here, we developed a multimodal approach to analyze cfDNA methylation and fragmentomic patterns in 273 BC patients, 108 individuals with benign breast conditions, and 134 healthy controls. Genome-wide analyses revealed distinct cfDNA copy number alterations and cytosine-enriched cleavage sites in BC patients. Targeted sequencing further revealed unique methylation patterns, including hypermethylation in GPR126 , KLF3 , and TLR10 and hypomethylation in TOP1 and MAFB . Our machine-learning model achieved an AUC of 0.90, with 93.6% specificity and 62.1–66.3% sensitivity for stage I–II cancers. In symptomatic populations, sensitivities were 50.0%, 68.2%, and 64.7% for BI-RADS categories 3, 4, and 5, respectively, with 96.1% specificity. Conclusions These findings underscore the potential of cfDNA biomarkers to enhance BC detection and reduce the rate of unnecessary biopsies.

Background Non-invasive multi-cancer early detection (MCED) tests have shown promise in enhancing early cancer detection. However, their clinical utility across diverse populations remains underexplored, limiting their routine implementation. This study aims to validate the clinical utility of a multimodal non-invasive circulating tumor DNA (ctDNA)-based MCED test, SPOT-MAS (Screening for the Presence Of Tumor by DNA Methylation And Size). Methods We conducted a multicenter prospective study, K-DETEK (ClinicalTrials.gov identifier: NCT05227261), involving 9057 asymptomatic individuals aged 40 years or older across 75 major hospitals and one research institute in Vietnam. Participants were followed for 12 months. Results Of the 9024 eligible participants, 43 (0.48%) tested positive for ctDNA. Among these, 17 were confirmed with malignant lesions in various primary organs through standard-of-care (SOC) imaging and biopsy, with 9 cases matching our tissue of origin (TOO) predictions. This resulted in a positive predictive value of 39.53% (95%CI 26.37–54.42) and a TOO accuracy of 52.94% (95%CI 30.96–73.83). Among the 8981 participants (99.52%) who tested negative, 8974 were confirmed cancer-free during a 12-month period after testing, yielding a negative predictive value of 99.92% (95% CI 99.84–99.96). The test demonstrated an overall sensitivity of 70.83% (95%CI 50.83–85.09) and a specificity of 99.71% (95% CI 99.58–99.80) for detecting various cancer types, including those without SOC screening options. Conclusions This study presents a prospective validation of a multi-cancer early detection (MCED) test conducted in a lower middle-income country, demonstrating the potential of SPOT-MAS for early cancer detection. Our findings indicate that MCED tests could be valuable additions to national cancer screening programs, particularly in regions where such initiatives are currently limited. Trial registration ClinicalTrials.gov ID: NCT05227261. Date of registration: 07/02/2022.

Vietnamese rural mountains are undergoing a significant transformation process in agriculture-based economy nowadays. A challenge facing ethnic minorities is identifying more appropriate way of life to cope with unpredictable risks associated with agriculture. This paper deals with livelihood diversification by the Dzao, an indigenous ethnic minority group in the Vietnamese Northern Mountains. One hundred and six Dzao households from 11 villages are surveyed with a systematic random sampling. The Simpson diversity index and the Average Agricultural Livelihood Diversification Index are used to measure the livelihood diversification of villages. Eighteen survey questions are developed from six determinants of livelihood diversification such as seasonality, risk strategies, labor markets, credit market failures, asset strategies and coping behavior and adaptation. The results show that livelihood diversification aligns Dzao farmers’ goals to the short-term economic feasibility and long-term sustainability. Farmers diversify their livelihood by combining livelihood strategies for agricultural intensification, agricultural extensification and migration. Diversification should range from a temporary change in the household livelihood portfolio (occasional diversification) to a deliberate attempt to optimize the household capacity taking advantage of opportunities and coping with unexpected constraints (strategic diversification).

This study reports the construction and validation of a CAFLUX (Chemically Activated Fluorescent Expression) HepG2 reporter cell line engineered to express a histone H2B–green fluorescent protein (H2B–GFP) fusion protein under the control of a dioxin-responsive cytochrome P450 1A1 (CYP1A1) promoter. A lentiviral construct containing a synthetic promoter with multiple dioxin-responsive elements (DREs) upstream of the H2B–EGFP coding sequence was cloned into the pFUGW vector, packaged in human embryonic kidney (HEK) 293FT cells, and used to transduce HepG2 hepatocellular carcinoma cells. Stable clones obtained by limiting dilution were screened for GFP expression in response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The resulting CAFLUX HepG2 cells exhibited dose-dependent nuclear GFP fluorescence when exposed to aryl hydrocarbon receptor (AhR) agonists, with limits of detection of approximately 0.01 pM for TCDD and 0.1 pM for benzo[a]pyrene (B[a]P), a polycyclic aromatic hydrocarbon (PAH). This reporter activity correlated with endogenous CYP1A1 mRNA expression as determined by quantitative polymerase chain reaction (qPCR), confirming that GFP signals reflected native transcriptional responses. In functional assays, curcumin suppressed GFP expression in a concentration-dependent manner and induced apoptotic morphology at higher doses, while extracellular vesicles (EVs) derived from adipose-derived stem cells (ADSCs) significantly reduced both GFP fluorescence and CYP1A1 mRNA levels, suggesting an inhibitory effect on AhR-driven transcription. The CAFLUX HepG2 reporter system therefore provides a sensitive and reproducible platform for real-time, nuclear-localized monitoring of AhR-mediated gene expression. Its responsiveness to both agonists and antagonists underscores its potential utility in toxicological evaluation, drug discovery, and the investigation of EV-mediated signaling in liver cancer models.

Career guidance, which strongly influences the world labor market and social structure, is studied in many countries on different levels. This study aims to explore the number, growth trajectories, and geographic distribution of studies on Career guidance in general schools and identify prominent influential authors, sources, publications, and new research issues. Bibliometric analysis is employed to analyze all 382 publications on the topic of Career guidance in general schools in the Scopus database. The data shows that the volume of scientific publications in this field remained steady from 1964 to 2010, while the period from 2010 to 2022 experienced a significant increase. There is a total of 2412 authors participating in research on this topic, from 1609 institutions, in 98 different countries and regions. Besides the studies of American, Italian, and Russian scholars, researchers in the Netherlands, the United Kingdom, and Australia also play a notable role in this topic. The most cited and notable journal on this topic is the British Journal of Guidance and Counseling. Recently, the most prevalent research topics include influencing general school students to prepare for their career readiness, and the opportunity to create social justice in understanding and deciding on career options.