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The prevailing paleobiogeographic hypothesis suggests that many steppe and tundra-steppe taxa currently found in Central Asia expanded into Europe during Pleistocene glacial periods, when open habitats dominated. However, previous studies have shown that one such species, the European narrow-headed vole ( Stenocranius anglicus ), diverged from its Asiatic counterparts over 200 thousand years ago, implying its prolonged isolation and survival in European refugia through the Eemian interglacial period. To test whether this was an exception or part of a broader pattern, we analysed the mitochondrial genomes from 33 Late Pleistocene and Holocene small hamster (Cricetinae) remains from Central and Western Europe, the Balkans, and Anatolia, all previously identified morphologically as grey dwarf hamster ( Nothocricetulus migratorius ). Contrary to expectations, 16 Late Pleistocene Central European samples were assigned to the hairy-footed hamster ( Cricetiscus sungorus ), a species currently restricted to northern Kazakhstan and southern Russia, whereas 17 samples from the Balkans and Anatolia belonged to the grey dwarf hamster. In both cases, the Late Pleistocene samples formed clades sister to modern populations; however, with relatively recent divergence times. This suggests population continuity or repeated expansion of hamsters from Asiatic sources rather than long-term isolation in Europe, as previously observed in narrow-headed voles. Our findings indicate that steppe species, despite occupying similar ecological niches, respond to past climate change in a species-specific manner. Therefore, broad generalisations regarding their evolutionary history may be misleading.

Background The field vole, an abundant and widespread microtine rodent, is a complex comprised of three cryptic species: the short-tailed field vole ( Microtus agrestis ) which is present over much of Eurasia, the Mediterranean field vole ( Microtus lavernedii ) in southern Europe, and the Portuguese field vole ( Microtus rozianus ) in western Spain and Portugal. Previous research has shown high genomic differentiation of these three lineages. However, the details of the process underlying their divergence remain unknown. Results We analyse 70 mitogenomes and 16 nuclear genomes of modern specimens, and 83 mitogenomes and 12 nuclear genomes of ancient specimens spanning the last 75 thousand years (ka). We estimate the divergence of Portuguese from short-tailed and Mediterranean field voles to be ca. 220 ka ago and of the latter two species to be ca. 110 ka ago, earlier than previous estimates involving only modern sequences. The divergence times we obtain match those between major mitochondrial lineages of cold-adapted and steppe rodents in Europe. We find signatures of gene flow within and between field vole lineages, with some analyses suggesting a hybrid origin of the Mediterranean lineage. Ancient specimens from the Italian Peninsula reveal a previously unrecognised lineage that show evidence of genetic exchange with other populations. Conclusions The pattern of genetic variation in the field vole species complex demonstrates the impact of stadial-interstadial cycles in generating recurrent episodes of allopatry and connectivity of populations, a situation which could only be revealed by our dense genomic sampling over time.

Grey voles (subgenus Microtus) represent a complex of at least seven closely related and partly cryptic species. The range of these species extends from the Atlantic to the Altai Mountains, but most of them occur east of the Black Sea. Using ancient DNA analyses of the Late Pleistocene specimens, we identified a new mtDNA lineage of grey voles in Europe. Phylogenetic analysis of mitochondrial DNA cytochrome b sequences from 23 voles from three caves, namely, Emine–Bair–Khosar (Crimea, Ukraine), Cave 16 (Bulgaria), and Bacho Kiro (Bulgaria), showed that 14 specimens form a previously unrecognized lineage, sister to the Tien Shan vole. The average sequence divergence of this lineage and the extant Tien Shan vole was 4.8%, which is similar to the divergence of grey vole forms, which are considered distinct species or being on the verge of speciation; M. arvalis and M. obscurus or M. mystacinus and M. rossiaemeridionalis. We estimated the time to the most recent common ancestor of the grey voles to be 0.66 Ma, which is over twice the recent estimates, while the divergence of the extant Tien Shan vole and the new lineage to be 0.29 Ma. Our discovery suggests that grey voles may have been more diversified in the past and that their ranges may have differed substantially from current ones. It also underlines the utility of ancient DNA to decipher the evolutionary history of voles. We sequenced mtDNA from multiple Late Pleistocene vole specimens from three caves near the Black Sea. We discovered a new divergent lineage of grey voles (subg. Microtus).

Taxonomic decisions made by palaeontologists are often based on a few morphological features preserved in the fossil material. This practice may sometimes lead to the description of new species based on single specimens, which are, in fact, extreme or aberrant morphological variants of known taxa. Ancient DNA (aDNA) analysis of the Late Pleistocene specimens from the archaeological site Petits Guinards (Creuzier-la-Vieux, Allier, France), described as a new vole Microtus (Sumeriomys) bifrons, did not confirm the species distinctness of the studied population. The genetically examined specimens belonged to Stenocranius anglicus and/or Microtus arvalis, the dominant species at the site. Our findings show that it is risky to describe new fossil taxa on the basis of phenotypic outliers or morphologically aberrant, rare specimens that do not fall within the previously known population variability. We also highlight the importance of ancient DNA in resolving taxonomic and nomenclature problems and classifying fossil mammals of the Late Pleistocene age.

Archaeological research at Ojców castle has yielded important information about life in that medieval castle. The results of zooarchaeological analyses presented in this paper complement the archaeological research, adding to our knowledge of the diet of the castle inhabitants from the time of establishment of the castle until the final residents. Zooarchaeological research is also complemented by data from older settlement phases on the castle hill, directly related to the Lusatian culture in the early Iron Age. The great variability of remains from mammals, birds, and fish and the taphonomic features of bones found in the different chronological strata of the castle’s courtyard reflect the diverse economic activities that took place in particular times and spaces.

There is a widespread belief that the abrupt warming at 14.7 ka had a profound impact on the environment. However, the direct correlation between the global climatic event and changes in local environments is not obvious. We examined faunal succession in an intra-mountain basin of the Western Carpathians to assess the potential influence of the climatic change between Greenland Stadial-2a and Greenland Interstadial-1e on the local environment. We investigated three vertebrate assemblages (total number of identified specimens = 18,745; minimum number of individuals = 7515; 138 taxa) from Obłazowa Cave (western entrance) and a Rock overhang in Cisowa Rock, radiocarbon dated to the period before and after the global warming, between ca. 17.0 and 14.0 ka. Our data revealed that the major abrupt warming that occurred 14.7 ka had little impact on the local environment, which could suggest that ecosystems in Central Europe were resilient to the abrupt global climate changes. The increase in fauna population sizes and species diversities in local biotopes was gradual and began long before the temperature increase. This was supported by the analysis of ancient DNA of Microtus arvalis , which showed a gradual increase in effective population size after 19.0 ka. The results of palaeoclimatic reconstruction pointed out that the compared sites were characterized by similar climatic conditions. According to our calculations, the differences in the annual mean temperatures did not exceed 0.5 °C and mean annual thermal amplitude changed from 22.9 to 22.4 °C. The environmental changes before 14.7 ka had no impact on the activity of Final Palaeolithic hunters in the studied area.

During the Late Pleistocene, narrow-headed voles ( Lasiopodomys gregalis ) inhabited Eurasia’s vast territories, frequently becoming the dominant small mammal species among steppe-tundra communities. We investigated the relationship between this species’ European and Asiatic populations by sequencing the mtDNA genomes of two extant specimens from Russia and 10 individuals from five Central European sites, dated to the post-LGM period. Phylogenetic analyses based on a large portion of mtDNA genomes highly supported the positioning of L. gregalis within Arvicolinae . The phylogeny based on mtDNA cytochrome b sequences revealed a deep divergence of European narrow-headed voles from Asiatic ones and their sister position against the extant L. gregalis and L. raddei . The divergence of the European lineage was estimated to a minimum 230 thousand years ago. This suggest, contrary to the current biogeographic hypotheses, that during the interglacial periods narrow-headed vole did not retreat from Europe but survived the unfavourable conditions within the refugial areas. Based on this result, we propose to establish a cryptic species status for the Late Pleistocene European narrow-headed vole and to name this taxon Lasiopodomys anglicus .

Determining the age of late Middle and Late Pleistocene (MIS 8-MIS 3) Neanderthal sites can be difficult as radiometry has limitations. Biostratigraphic methods can be used to overcome these difficulties. A species useful in these analyses is Alexandromys oeconomus (Pallas, 1776), which has a wide modern geographical distribution in the Northern Hemisphere. During the Pleistocene, its range significantly increased, occurring in large numbers both in interglacials and glacials. We distinguished Middle Pleistocene from Late Pleistocene and modern populations of this species based on temporal variation in the morphological structure of the first lower molar (m1) occlusion surface, and the relative proportions of the typical “ oeconomus ” and atypical “ gud ,” “ nivalis ,” and “ malei ” morphotypes. Our results showed that the mean values of metric parameters of the occlusion surface m1 of A. oeconomus varied over time. We found that this variation distinguisheed between populations of species from the late Middle Pleistocene (MIS 8-MIS 6) and early Late Pleistocene (MIS 5) from those from the Late Pleistocene (MIS 4-MIS 2), and some measurements and coefficients were correlated with climate-environmental changes. Using variation in A. oeconomus m1 morphology can be used as a tool in biostratigraphy requires further research including, among others, studies on how morphology varies between regions.

New research at the Doroshivtsi site in Ukraine has provided data that allow fresh insights into a well-known and important Gravettian site in the Middle Dniester Valley. Keywords: Ukraine, Dniester gorge, Gravettian, LGM settlement, lithics, colluvial sediments

This article discusses the objectives of the Stone Age Man in Caves of the Tatra Mountains project, which aims to explain the mysterious absence of evidence for the Palaeolithic in the Tatra Mountains of Eastern Europe. We present preliminary work from Hučivá Cave, which demonstrates clear traces of Magdalenian settlement within this region.