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Britain's history runs deep - and much of its treasured past lies hidden underfoot. Every day, new discoveries are unearthed not by expert archaeologists but by amateur enthusiasts. Armed with spades and maps, these detectorists, mudlarks and fieldwalkers scour the earth's surface. What they find is perhaps at first glance unremarkable: fragments of pottery, metal coins, jewellery. But from the pieces emerge stories of how our ancestors lived, traded, ate, played, fought - and died. 'Beneath Our Feet' is a celebration of this growing public involvement in archaeology.

Biopharmaceuticals have revolutionized the field of medicine in the types of active ingredient molecules and treatable indications. Adoption of Quality by Design and Process Analytical Technology (PAT) frameworks has helped the biopharmaceutical field to realize consistent product quality, process intensification, and real-time control. As part of the PAT strategy, Raman spectroscopy offers many benefits and is used successfully in bioprocessing from single-cell analysis to cGMP process control. Since first introduced in 2011 for industrial bioprocessing applications, Raman has become a first-choice PAT for monitoring and controlling upstream bioprocesses because it facilitates advanced process control and enables consistent process quality. This paper will discuss new frontiers in extending these successes in upstream from scale-down to commercial manufacturing. New reports concerning the use of Raman spectroscopy in the basic science of single cells and downstream process monitoring illustrate industrial recognition of Raman’s value throughout a biopharmaceutical product’s lifecycle. Finally, we draw upon a nearly 90-year history in biological Raman spectroscopy to provide the basis for laboratory and in-line measurements of protein quality, including higher-order structure and composition modifications, to support formulation development.

Short chain fatty acids (SCFAs; including acetate, propionate, and butyrate) are an important class of biological molecules that play a major role in modulating host-microbiome interactions. Despite significant research into SCFA-mediated biological mechanisms, absolute quantification of these molecules in their native form by liquid chromatography mass spectrometry is challenging due to their relatively poor chromatographic properties. Herein, we introduce SQUAD, an isotope-based strategy for absolute quantification of SCFAs in complex biological samples. SQUAD uses aniline derivatization in conjunction with isotope dilution and analysis by reverse-phase liquid chromatography mass spectrometry. We show that SQUAD enables absolute quantification of biologically relevant SCFAs in complex biological samples with a lower limit of detection of 40 nM and a lower limit of quantification ranging from 160 nM to 310 nM. We observed an intra- and inter-day precision under 3% (relative standard deviation) and errors in intra- and inter-day accuracy under 10%. To demonstrate this quantification strategy, we analyzed SCFAs in the caecal contents of germ free versus conventionally raised specific pathogen free (SPF) mice. We showed that acetate was the most abundant SCFA in both types of mice and was present at 200-fold higher concentration in the SPF mice. We also illustrated the use of our quantification strategy in in vitro microbial cultures from five different species of bacteria grown in Mueller Hinton media. This study illustrates the diverse SCFA production rates across microbial taxa with acetate production serving as one of the key differentiating factors across the species. In summary, we introduce an isotope dilution strategy for absolute quantification of aniline-dativized SCFAs and illustrate the utility of this approach for microbiome research.

A bstract Most searches for top partners, T , are concerned with top partner pair production. However, as these bounds become increasingly stringent, the LHC energy will saturate and single top partner production will become more important. In this paper we study the novel signature of the top partner produced in association with the SM top, pp → T t ¯ + t T ¯ , in a model where the Standard Model (SM) is extended by a vector-like SU(2) L singlet fermion top partner and a real, SM gauge singlet scalar, S . In this model, pp → T t ¯ + t T ¯ production is possible through loops mediated by the scalar singlet. We find that, with reasonable coupling strengths, the production rate of this channel can dominate top partner pair production at top partner masses of m T ≳ 1 . 5 TeV. In addition, this model allows for the exotic decay modes T → tg , T → t γ, and T → tS . In much of the parameter space the loop induced decay T → tg dominates and the top partner is quite long lived. New search strategies are necessary to cover these decay modes. We project the the sensitivity of the high luminosity LHC to pp → T t ¯ + t T ¯ via a realistic collider study. We find with 3 ab −1 , the LHC is sensitive to this process for masses m T ≲ 2 TeV. In addition, we provide appendices detailing the renormalization of this model.

A bstract We analyze the collider signatures of the real singlet extension of the Standard Model in regions consistent with a strong first-order electroweak phase transition and a singlet-like scalar heavier than the Standard Model-like Higgs. A definitive correlation exists between the strength of the phase transition and the trilinear coupling of the Higgs to two singlet-like scalars, and hence between the phase transition and non-resonant scalar pair production involving the singlet at colliders. We study the prospects for observing these processes at the LHC and a future 100 TeV pp collider, focusing particularly on double singlet production. We also discuss correlations between the strength of the electroweak phase transition and other observables at hadron and future lepton colliders. Searches for non-resonant singlet-like scalar pair production at 100 TeV would provide a sensitive probe of the electroweak phase transition in this model, complementing resonant di-Higgs searches and precision measurements. Our study illustrates a strategy for systematically exploring the phenomenologically viable parameter space of this model, which we hope will be useful for future work.

A bstract We present a comprehensive study on how to distinguish the properties of heavy dijet resonances at hadron colliders. A variety of spins, chiral couplings, charges, and QCD color representations are considered. Distinguishing the different color representations is particularly difficult at hadron colliders. To determine the QCD color structure, we consider a third jet radiated in a resonant dijet event. We show that the relative rates of three-jet versus two-jet processes are sensitive to the color representation of the resonance. We also show analytically that the antennae radiation pattern of soft radiation depends on the color structure of dijet events and develops an observable that is sensitive to the antennae patterns. Finally, we exploit a Convolutional Neural Network with Machine Learning techniques to differentiate the radiation patterns from different colored resonances and find encouraging results to discriminate them. We demonstrate our results numerically at a 14 TeV LHC, and the methodology presented here should be applicable to other future hadron colliders.

The geological heritage of Australia’s landscapes and World Heritage areas has generally been underpromoted to the public by the tourism industry. However, in 2008, the fledgling world of geotourism in Australia received a significant boost with two events: the Inaugural Global Geotourism Conference ‘Discover the Earth beneath our Feet’ held in Fremantle, Western Australia, and the declaration of the UNESCO Kanawinka Global Geopark, which linked volcanic regions in South Australia and Victoria. Simultaneously the Australian Federal Government launched the ‘Australian National Landscapes’ (ANL) program. However, this impetus was not sustained when the Kanawinka Global Geopark was deregistered as a UNESCO-branded geopark in 2012, and the ANL program faded within a decade. Despite these setbacks, as an outcome of the 2008 Fremantle conference, several productive lines of geotourism have developed across Australia. This paper reviews the history of Australian geotourism since 2008. It examines the impacts of the experiences, lessons learned, problems for geology as perceived by National Parks and the Environment movement, geological communication problems, and the subsequent evolution of Australian geotourism. From these issues, new non-government bodies and initiatives have arisen, including the Australian Geoparks Network, the Australian Geoscience Council, and the recent development of a National Geotourism Strategy. Strong elements emerging from these initiatives are the increasing development of geotrails (which suit the large Australian continent) and the new Australian concept of ‘GeoRegions’. These are in response to an awareness that geotourism requires a flexible outlook to widen the appreciation and appeal of geological heritage and landscapes to the broader public. A further new direction is suggested: for Australian geotourism to combine with some elements of ICOMOS Cultural Routes. An outstanding example, the ICOMOS Overland Telegraph Line (OTL) Cultural Route that crosses Australia from south to north, is considered. For 2000 km, the construction of this line in the 1870s followed the regional geology and hydrology, relying upon the available biota but bringing about a clash of human cultures. The six colonies of Australia were finally linked to the world by wire, but the arrival of the OTL had a significant impact on the country’s Indigenous inhabitants. In Australia and globally, geotourism is incorporating the A–B–Cs (abiotic, biotic and cultural elements) to more effectively encourage the public to value their landscapes and the associated stories. The OTL provides an example of a newly introduced fourth dimension for geotourism, which gives consideration to the socio-political context of landscape adaptation.

A bstract We study the top-Higgs coupling with a CP violating phase ξ at a future multi-TeV muon collider. We focus on processes that are directly sensitive to the top quark Yukawa coupling: t t ¯ h , tbhμν , and t t ¯ hν ν ¯ with h → b b ¯ and semileptonic top decays. At different energies, different processes dominate the cross section, providing complementary information. At and above an energy of O 10 TeV, vector boson fusion processes dominate. As we show, in the Standard Model there is destructive interference in the vector boson fusion processes t t ¯ hν ν ¯ and tbhμν between the top quark Yukawa and Higgs-gauge boson couplings. A CP-violating phase changes this interference, and the cross section measurement is very sensitive to the size of the CP-violating angle. Although we find that the cross sections are measured to O 50 % statistical uncertainty at 1 σ , a 10 and 30 TeV muon collider can bound the CP-violating angle | ξ | ≲ 9.0 ° and | ξ | ≲ 5.4 ° , respectively. However, cross section measurements are insensitive to the sign of the CP-violating angle. To determine that the coupling is truly CP violating, observables sensitive to CP-violation must be measured. We find in the t t ¯ h process the azimuthal angle between the t + t ¯ plane and the initial state muon+Higgs plane shows good discrimination for ξ = ±0.1 π . For the tbhμν and t t ¯ hν ν ¯ processes, the operator proportional to p → μ × p → h · p → t is sensitive to the sign of CP phase ξ . From these observables, we construct asymmetry parameters that show good distinction between different values and signs of the CP violating angle.

The lack of microbial genomes and isolates from the deep seabed means that very little is known about the ecology of this vast habitat. Here, we investigate energy and carbon acquisition strategies of microbial communities from three deep seabed petroleum seeps (3 km water depth) in the Eastern Gulf of Mexico. Shotgun metagenomic analysis reveals that each sediment harbors diverse communities of chemoheterotrophs and chemolithotrophs. We recovered 82 metagenome-assembled genomes affiliated with 21 different archaeal and bacterial phyla. Multiple genomes encode enzymes for anaerobic oxidation of aliphatic and aromatic compounds, including those of candidate phyla Aerophobetes, Aminicenantes, TA06 and Bathyarchaeota. Microbial interactions are predicted to be driven by acetate and molecular hydrogen. These findings are supported by sediment geochemistry, metabolomics, and thermodynamic modelling. Overall, we infer that deep-sea sediments experiencing thermogenic hydrocarbon inputs harbor phylogenetically and functionally diverse communities potentially sustained through anaerobic hydrocarbon, acetate and hydrogen metabolism. Little is known about the microbial ecology of the deep seabed. Here, Dong et al. predict metabolic capabilities and microbial interactions in deep seabed petroleum seeps using shotgun metagenomics, sediment geochemistry, metabolomics, and thermodynamic modelling.