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Background Long non-coding RNAs (lncRNAs) have been reported to have a crucial impact on the pathogenesis of acute myeloid leukemia (AML). Cuproptosis, a copper-triggered modality of mitochondrial cell death, might serve as a promising therapeutic target for cancer treatment and clinical outcome prediction. Nevertheless, the role of cuproptosis-related lncRNAs in AML is not fully understood. Methods The RNA sequencing data and demographic characteristics of AML patients were downloaded from The Cancer Genome Atlas database. Pearson correlation analysis, the least absolute shrinkage and selection operator algorithm, and univariable and multivariable Cox regression analyses were applied to identify the cuproptosis-related lncRNA signature and determine its feasibility for AML prognosis prediction. The performance of the proposed signature was evaluated via Kaplan–Meier survival analysis, receiver operating characteristic curves, and principal component analysis. Functional analysis was implemented to uncover the potential prognostic mechanisms. Additionally, quantitative real-time PCR (qRT-PCR) was employed to validate the expression of the prognostic lncRNAs in AML samples. Results A signature consisting of seven cuproptosis-related lncRNAs (namely NFE4, LINC00989, LINC02062, AC006460.2, AL353796.1, PSMB8-AS1, and AC000120.1) was proposed. Multivariable cox regression analysis revealed that the proposed signature was an independent prognostic factor for AML. Notably, the nomogram based on this signature showed excellent accuracy in predicting the 1-, 3-, and 5-year survival (area under curve = 0.846, 0.801, and 0.895, respectively). Functional analysis results suggested the existence of a significant association between the prognostic signature and immune-related pathways. The expression pattern of the lncRNAs was validated in AML samples. Conclusion Collectively, we constructed a prediction model based on seven cuproptosis-related lncRNAs for AML prognosis. The obtained risk score may reveal the immunotherapy response in patients with this disease.

HighlightsNaturally crosslinked carbonaceous liquid metal aqueous printable ink mediated by biopolymers.E-textile with conductivity, stability, wearability, and aesthetic characteristics.Multi-applications in health monitoring, pressure sensing, and energy harvesting.Achieving flexible electronics with comfort and durability comparable to traditional textiles is one of the ultimate pursuits of smart wearables. Ink printing is desirable for e-textile development using a simple and inexpensive process. However, fabricating high-performance atop textiles with good dispersity, stability, biocompatibility, and wearability for high-resolution, large-scale manufacturing, and practical applications has remained challenging. Here, water-based multi-walled carbon nanotubes (MWCNTs)-decorated liquid metal (LM) inks are proposed with carbonaceous gallium–indium micro-nanostructure. With the assistance of biopolymers, the sodium alginate-encapsulated LM droplets contain high carboxyl groups which non-covalently crosslink with silk sericin-mediated MWCNTs. E-textile can be prepared subsequently via printing technique and natural waterproof triboelectric coating, enabling good flexibility, hydrophilicity, breathability, wearability, biocompatibility, conductivity, stability, and excellent versatility, without any artificial chemicals. The obtained e-textile can be used in various applications with designable patterns and circuits. Multi-sensing applications of recognizing complex human motions, breathing, phonation, and pressure distribution are demonstrated with repeatable and reliable signals. Self-powered and energy-harvesting capabilities are also presented by driving electronic devices and lighting LEDs. As proof of concept, this work provides new opportunities in a scalable and sustainable way to develop novel wearable electronics and smart clothing for future commercial applications.

The aim of this study was to assess the relationship between red cell distribution width and albumin ratio (RAR) inflammatory markers and prevalence of osteoarthritis in a diabetic population by analyzing data from the National Health and Nutrition Examination Survey (NHANES). Using data from 2005 to 2018, using multivariate logistic regression, generalized additive models, and smooth curve fitting, we investigated the connection between RAR and osteoarthritis in diabetic individuals. Robustness checks included subgroup analyses. A total of 2698 participants were included in this study, in which the prevalence of osteoarthritis was 24.20%. After controlling for variables, multivariate logistic regression analysis revealed a significant and positive correlation between RAR levels and the prevalence of osteoarthritis (OR = 1.52, 95% CI: 1.24, 1.88). Smoothing curve analysis showed a significant nonlinear relationship between RAR levels and the prevalence of osteoarthritis (log likelihood ratio test P  < 0.05). Threshold effect analysis showed an inflection point of 3.69 for RAR, and to the left of the inflection point, the OR for prevalence of osteoarthritis was 2.61 (95% CI: 1.77, 3.83) for each unit increase in RAR; interaction analyses showed that the variables did not have a significant effect on this relationship ( p  > 0.05 for all interactions). Elevated RAR is associated with an increased prevalence of osteoarthritis in diabetic patients. This study supports the hypothesis of an association between RAR and osteoarthritis in the U.S. diabetic population, and diabetic individuals with elevated RAR levels should be particularly aware of their increased risk of developing osteoarthritis.

With burgeoning considerations over energy issues and carbon emissions, energy harvesting devices such as triboelectric nanogenerators (TENGs) are developed to provide renewable and sustainable power. Enhancing electric output and other properties of TENGs during operation is the focus of research. Herein, two species (Nb2S2C and Ta2S2C) of a new family of 2D materials, Transition Metal Carbo‐Chalcogenides (TMCCs), are first employed to develop TENGs with doping into Polydimethylsiloxane (PDMS). Compared with control samples, these two TMCC‐based TENGs exhibit higher electric properties owing to the enhanced permittivity of PDMS composite, and the best performance is achieved at a concentration of 3 wt. ‰ with open circuit voltage (Voc) of 112 V, short circuit current (Isc) of 8.6 µA and charge transfer (Qsc) of 175 nC for Nb2S2C based TENG, and Voc of 127 V, Isc of 9.6 µA, and Qsc of 230 nC for Ta2S2C based TENGs. These two TENGs show a maximum power density of 1360 and 911 mW m−2 respectively. Moreover, the tribology performance is also evaluated with the same materials, revealing that the Ta2S2C/PDMS composite as the electronegative material presented a lower coefficient of friction (COF) than the Nb2S2C/PDMS composite. Their applications for energy harvesting and self‐powered sensing are also demonstrated. Transition metal carbo‐chalcogenide (TMCC), Nb2S2C and Ta2S2C, are first employed to fabricate triboelectric nanogenerator (TENG) with polydimethylsiloxane (PDMS).TMCC‐TENGs exhibit enhanced electrical properties with optimum value both at 3 wt.‰. Maximum power density reaches 1360 mW m−2 for Nb2S2C‐TENG and 911 mW m−2 for Ta2S2C‐TENG. Ta2S2C/PDMS composite exhibits reduced COF compared to Nb2S2C/PDMS.

Osteoarthritis (OA) is marked by cartilage deterioration, subchondral bone changes, and an inflammatory microenvironment. The study introduces the Microneedle‐Delivered Polydopamine‐Exosome (PDA@Exo MN), a therapeutic that not only preserves cartilage and promotes bone regeneration but also improves localized drug delivery through enhanced penetration capabilities. PDA@Exo MN shows strong reactive oxygen species (ROS) scavenging abilities and high biocompatibility, fostering osteogenesis and balancing anabolic and catabolic processes in cartilage. It directs macrophage polarization from M0 to the anti‐inflammatory M2 phenotype. RNA sequencing of treated chondrocytes demonstrates restored cellular function and activated antioxidant responses, with modulated inflammatory pathways. The PI3K‐AKT‐mTOR pathway's activation, essential for PDA@Exo's effects, is confirmed via bioinformatics and Western blot. In vivo assessments robustly validate that PDA@Exo MN prevents cartilage degradation and OA progression, supported by histological assessments and micro‐CT analysis, highlighting its disease‐modifying impact. The excellent biocompatibility of PDA@Exo MN, verified through histological (H&E) and blood tests showing no organ damage, underscores its safety and efficacy for OA therapy, making it a novel and multifunctional nanomedical approach in orthopedics, characterized by organ‐friendliness and biosecurity. Introducing Microneedle‐Delivered Polydopamine‐Exosome (PDA@Exo MN), a novel osteoarthritis therapeutic with enhanced drug delivery via improved penetration. Demonstrating strong antioxidative properties and high biocompatibility, PDA@Exo MN preserves cartilage, promotes bone regeneration, and effectively modulates inflammatory pathways to halt disease progression.

Alveolar echinococcosis (AE) is a highly lethal helminth infection. Current chemotherapeutic strategies for AE primarily involve the use of benzimidazoles (BZs) such as mebendazole (MDZ) and albendazole (ABZ), which exhibit limited efficacy. In a previous study, the vaccine of recombinant Echinococcus granulosus P29 (r Eg P29) showed significant immunoprotection against E . granulosus in both mice and sheep. In the current study, we utilized hybridoma technology to generate five monoclonal antibodies (mAbs) against P29, among which 4G10F4 mAb exhibited the highest antigen-specific binding capacity. This mAb was selected for further investigation of anti-AE therapy, both in vivo and in vitro. In vitro, 4G10F4 inhibited a noteworthy inhibition of E . multilocularis protoscoleces and primary cells viability through complement-dependent cytotoxicity (CDC) mechanism. In vivo, two experiments were conducted. In the first experiment, mice were intraperitoneally injected with Em protoscoleces, and subsequently treated with 4G10F4 mAb (2.5/5/10 mg/kg) at 12 weeks postinfection once per week for 8 times via tail vein injection. Mice that were treated with 4G10F4 mAb only in dosage of 5mg/kg exhibited a significant lower mean parasite burden (0.89±0.97 g) compared to isotype mAb treated control mice (2.21±1.30 g). In the second experiment, mice were infected through hepatic portal vein and treated with 4G10F4 mAb (5mg/kg) at one week after surgery once per week for 8 times. The numbers of hepatic metacestode lesions of the 4G10F4 treatment group were significantly lower in comparison to the isotype control group. Pathological analysis revealed severe disruption of the inner structure of the metacestode in both experiments, particularly affecting the germinal and laminated layers, resulting in the transformation into infertile vesicles after treatment with 4G10F4. In addition, the safety of 4G10F4 for AE treatment was confirmed through assessment of mouse weight and evaluation of liver and kidney function. This study presents antigen-specific monoclonal antibody immunotherapy as a promising therapeutic approach against E . multilocularis induced AE.

This study aimed to explore the potential categorization of parental psychological flexibility (PPF) among working mothers, examine the presence of group heterogeneity in their PPF, and investigate differences in PPF and social support across different PPF categories. A total of 773 working mothers were surveyed using the Parental Psychological Flexibility Questionnaire and the Social Support Scale, with latent profile analysis (LPA) employed to test for heterogeneity. The results indicated that the PPF of working mothers could be classified into three distinct latent profiles: the conflict group (Class 1, 52.8%), the avoidance group (Class 2, 4.5%), and the flexible group (Class 3, 42.7%). Significant differences were observed in both PPF ( F  = 798.936, p  < 0.001) and social support (F = 35.102, p  < 0.001) across the three profiles. These findings emphasize the necessity of targeted interventions (e.g., mindfulness training for the avoidance group) and policy support (e.g., workplace flexibility measures) to enhance PPF and social support among working mothers, thereby promoting family well-being.

3-Hydroxybutyrate (3HB) is a small ketone body molecule produced endogenously by the body in the liver. Previous studies have shown that 3HB can reduce blood glucose level in type 2 diabetic (T2D) patients. However, there is no systematic study and clear mechanism to evaluate and explain the hypoglycemic effect of 3HB. Here we demonstrate that 3HB reduces fasting blood glucose level, improves glucose tolerance, and ameliorates insulin resistance in type 2 diabetic mice through hydroxycarboxylic acid receptor 2 (HCAR2). Mechanistically, 3HB increases intracellular calcium ion (Ca 2+ ) levels by activating HCAR2, thereby stimulating adenylate cyclase (AC) to increase cyclic adenosine monophosphate (cAMP) concentration, and then activating protein kinase A (PKA). Activated PKA inhibits Raf1 proto-oncogene serine/threonine-protein kinase (Raf1) activity, resulting in a decrease in extracellular signal-regulated kinases 1/2 (ERK1/2) activity and ultimately inhibiting peroxisome proliferator-activated receptor γ (PPARγ) Ser273 phosphorylation in adipocytes. Inhibition of PPARγ Ser273 phosphorylation by 3HB altered the expression of PPARγ regulated genes and reduced insulin resistance. Collectively, 3HB ameliorates insulin resistance in type 2 diabetic mice through a pathway of HCAR2/Ca 2+ /cAMP/PKA/Raf1/ERK1/2/PPARγ.

Osteosarcoma, recognized for its aggressiveness and resistance to chemotherapy, notably doxorubicin, poses significant treatment challenges. This comprehensive study investigated the CXCR4–CARM1–YAP signaling axis and its pivotal function in controlling aerobic glycolysis, which plays a crucial role in doxorubicin resistance. Detailed analysis of Dox‐resistant 143b/MG63‐DoxR cells has uncovered the overexpression of CXCR4. Utilizing a combination of molecular biology techniques including gene silencing, aerobic glycolysis assays such as Seahorse experiments, RNA sequencing, and immunofluorescence staining. The study provides insight into the mechanistic pathways involved. Results demonstrated that disrupting CXCR4 expression sensitizes cells to doxorubicin‐induced apoptosis and alters glycolytic activity. Further RNA sequencing revealed that CARM1 modulated this effect through its influence on glycolysis, with immunofluorescence of clinical samples confirming the overexpression of CXCR4 and CARM1 in drug‐resistant tumors. Chromatin immunoprecipitation studies further highlighted the role of CARM1, showing it to be regulated by methylation at the H3R17 site, which in turn affected YAP expression. Crucially, in vivo experiments illustrated that CARM1 overexpression could counteract the tumor growth suppression that resulted from CXCR4 inhibition. These insights revealed the intricate mechanisms at play in osteosarcoma resistance to doxorubicin and pointed toward potential new therapeutic strategies that could target this metabolic and signaling network to overcome drug resistance and improve patient outcomes. Osteosarcoma, a highly aggressive bone tumor, frequently leads to poor prognoses for patients, often due to drug resistance, particularly to doxorubicin (Dox). The study highlights the significant role of the CXCR4–CARM1–YAP axis in regulating aerobic glycolysis and thereby modulating drug resistance in osteosarcoma.

Cardiovascular implantable electronic devices (CIEDs) face dual challenges of high‐frequency electromagnetic interference and functional integration. This work reports a multifunctional material constructed via a double‐network ionic hydrogel strategy, enabling the integrated realization of efficient electromagnetic shielding and self‐powered physiological monitoring. An interpenetrating network skeleton is formed through physical crosslinking of sodium alginate (SA) with Ca2⁺ and in situ polymerization of acrylamide (AM). By regulating the specific coordination of ions to induce directional channels and synergistically regulating salt concentration with hydration, an absorption‐dominated shielding mechanism centered on ion polarization‐interface relaxation is established. The optimized h‐CA‐PAM‐Li⁺‐1.0 hydrogel exhibits an electromagnetic interference (EMI) shielding effectiveness (SET) of 63.75 dB in the X‐band, with absorption loss accounting for over 93%. Leveraging the excellent ionic conductivity of the hydrogel, a self‐powered sensor encapsulated in PDMS films and integrated with wireless modules is fabricated, capable of real‐time capture of physiological signals such as heartbeat while maintaining high sensitivity and anti‐interference capability in dynamic environments. Free of traditional conductive fillers, this material combines biocompatibility, low cost, and designability, providing a material‐device‐system integrated solution for electromagnetic protection and intelligent monitoring of implantable electronic devices and opening a new research paradigm for multifunctional shielding materials. A multifunctional dual network ionic hydrogel is used to address the challenges of EMI and health detection integration for CIEDs. An absorption‐based EMI shielding mechanism is achieved through ion polarization/interfacial relaxation. With an optimal SE of 63.75 dB and absorption loss greater than 93%, the wirelessly integrated self‐powered sensor in a PDMS package captures physiological signals.