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"Liang, Yu Ming"
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Diagnostic role of multislice spiral computed tomography combined with clinical manifestations and laboratory tests in acute appendicitis subtypes
The study aimed to investigate the diagnostic role of multislice spiral CT (MSCT) combined with clinical manifestations and laboratory tests in acute appendicitis subtypes. Patients diagnosed with acute appendicitis were included for retrospective analysis and their clinical manifestations and MSCT signs were analyzed. The clinical manifestations of different subtypes of acute appendicitis, including simple appendicitis, suppurative appendicitis and gangrenous appendicitis, were compared. The clinical manifestations were anorexia in 51.1% of patients, nausea and vomiting in 62.0%, shifting right lower abdominal pain in 51.1%, elevated body temperature in 31.2%, right lower quadrant abdominal tenderness in 91.4%, rebound tenderness in 91.4%, increased white cell count in 89.1%, high neutrophil count in 88.2%, increased appendiceal diameter enlargement in 100%, surrounding exudate in 95.0%, fecal stones in 51.6%, appendiceal wall thickening in 94.6%, lymph node in 82.8% and intestinal stasis in 18.6%. There were statistically significant differences in body temperature and neutrophil percentage among the subtypes of appendicitis and they were lowest in simple appendicitis and highest in gangrenous appendicitis. There were statistically significant differences in appendix diameter and the surrounding exudate among the subtypes of appendicitis and they were lowest in simple appendicitis and highest in gangrenous appendicitis. Clinical manifestations and MSCT signs, especially body temperature, percentage of neutrophils and the surrounding exudate, might have significant diagnostic value in acute appendicitis.
Journal Article
Gene regulation of anthocyanin biosynthesis in two blood-flesh peach (Prunus persica (L.) Batsch) cultivars during fruit development
The blood-flesh peach has become popular in China due to its attractive anthocyanin-induced pigmentation and antioxidant properties. In this study, we investigated the molecular mechanisms underlying anthocyanin accumulation by examining the expression of nine genes of the anthocyanin biosynthesis pathway found in the peach mesocarp. Expression was measured at six developmental stages in fruit of two blood-flesh and one white-flesh peach cultivars, using quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results show that the expression of the chalcone synthase (
CHS
) gene was closely related to anthocyanin accumulation in both of the blood-flesh peaches. In the white-flesh peach, we found that the transcription level of phenylalanine ammonia-lyase (
PAL
) during fruit development was much lower than that in the blood-flesh peach, even though all other genes of the anthocyanin biosynthesis pathway were highly expressed, suggesting that the
PAL
gene may be limiting in anthocyanin production in the white-flesh peach. Moreover, the transcription levels of the
CHS
and UDP-glucose-flavonoid 3-
O
-glucosyltransferase (
UFGT
) genes were markedly up-regulated at three days after bag removal (DABR) in the blood-flesh peach, suggesting that
CHS
and
UFGT
are the key genes in the process of anthocyanin biosynthesis for both of the blood-flesh peaches. The present study will be of great help in improving understanding of the molecular mechanisms involved in anthocyanin accumulation in blood-flesh peaches.
Journal Article
Peach genetic resources: diversity, population structure and linkage disequilibrium
Doc number: 84 Abstract Background: Peach (Prunus persica (L.) Batsch) is one of the most important model fruits in the Rosaceae family. Native to the west of China, where peach has been domesticated for more than 4,000 years, its cultivation spread from China to Persia, Mediterranean countries and to America. Chinese peach has had a major impact on international peach breeding programs due to its high genetic diversity. In this research, we used 48 highly polymorphic SSRs, distributed over the peach genome, to investigate the difference in genetic diversity, and linkage disequilibrium (LD) among Chinese cultivars, and North American and European cultivars, and the evolution of current peach cultivars. Results: In total, 588 alleles were obtained with 48 SSRs on 653 peach accessions, giving an average of 12.25 alleles per locus. In general, the average value of observed heterozygosity (0.47) was lower than the expected heterozygosity (0.60). The separate analysis of groups of accessions according to their origin or reproductive strategies showed greater variability in Oriental cultivars, mainly due to the high level of heterozygosity in Chinese landraces. Genetic distance analysis clustered the cultivars into two main groups: one included four wild related Prunus , and the other included most of the Oriental and Occidental landraces and breeding cultivars. STRUCTURE analysis assigned 469 accessions to three subpopulations: Oriental (234), Occidental (174), and Landraces (61). Nested STRUCTURE analysis divided the Oriental subpopulation into two different subpopulations: 'Yu Lu' and 'Hakuho'. The Occidental breeding subpopulation was also subdivided into nectarine and peach subpopulations. Linkage disequilibrium (LD) analysis in each of these subpopulations showed that the percentage of linked (r2 > 0.1) intra-chromosome comparisons ranged between 14% and 47%. LD decayed faster in Oriental (1,196 Kbp) than in Occidental (2,687 Kbp) samples. In the 'Yu Lu' subpopulation there was considerable LD extension while no variation of LD with physical distance was observed in the landraces. From the first STRUCTURE result, LG1 had the greatest proportion of alleles in LD within all three subpopulations. Conclusions: Our study demonstrates a high level of genetic diversity and relatively fast decay of LD in the Oriental peach breeding program. Inclusion of Chinese landraces will have a greater effect on increasing genetic diversity in Occidental breeding programs. Fingerprinting with genotype data for all 658 cultivars will be used for accession management in different germplasms. A higher density of markers are needed for association mapping in Oriental germplasm due to the low extension of LD. Population structure and evaluation of LD provides valuable information for GWAS experiment design in peach.
Journal Article
Human action segmentation and classification based on the Isomap algorithm
Visual analysis of human behavior has attracted a great deal of attention in the field of computer vision because of the wide variety of potential applications. Human behavior can be segmented into atomic actions, each of which indicates a single, basic movement. To reduce human intervention in the analysis of human behavior, unsupervised learning may be more suitable than supervised learning. However, the complex nature of human behavior analysis makes unsupervised learning a challenging task. In this paper, we propose a framework for the unsupervised analysis of human behavior based on manifold learning. First, a pairwise human posture distance matrix is derived from a training action sequence. Then, the isometric feature mapping (Isomap) algorithm is applied to construct a low-dimensional structure from the distance matrix. Consequently, the training action sequence is mapped into a manifold trajectory in the Isomap space. To identify the break points between the trajectories of any two successive atomic actions, we represent the manifold trajectory in the Isomap space as a time series of low-dimensional points. A temporal segmentation technique is then applied to segment the time series into sub series, each of which corresponds to an atomic action. Next, the dynamic time warping (DTW) approach is used to cluster atomic action sequences. Finally, we use the clustering results to learn and classify atomic actions according to the nearest neighbor rule. If the distance between the input sequence and the nearest mean sequence is greater than a given threshold, it is regarded as an unknown atomic action. Experiments conducted on real data demonstrate the effectiveness of the proposed method.
Journal Article
PpLTP1 Primary Allergen Gene is Highly Conserved in Peach and Has Small Variations in Other Prunus Species
Peach lipid transfer protein (LTP1), Pru p 3.01, is a major allergen causing severe systemic reactions in peach allergic patients in Mediterranean countries and China. Significant expression variability has been reported among peach cultivars at both transcript and protein level. In this study, the allele diversity of the LTP1-encoding gene was assessed in a large set of peach cultivars by direct sequencing of the gene and its upstream region. Evolution of the LTP1-encoding genes in peach (Prunus persica) and three other Prunus species (Prunus kansuensis, Prunus mira, and Prunus davidiana) were also inferred. Sequence analysis revealed that LTP1-encoding genes are highly conserved among peach cultivars and wild peach. Three different allele sequences were obtained from 50 Prunus accessions on the basis of the upstream region of the LTP1-encoding gene and three allele specific markers were derived according to the polymorphic sites. These markers were used to test 316 peach cultivars, most of which clustered within the three main subpopulations of peach, ‘Yu Lu’, ‘Hakuho’ and landraces. The genotypic frequencies and allele frequencies in the oriental peach cultivars in these subpopulations except for ‘Yu Lu’ were found to be in Hardy-Weinberg equilibrium (P < 0.05). The dominant alleles were upLTP1-a in the ‘Yu Lu’ subpopulation and upLTP1-c in the ‘Hakuho’ subpopulation, allele upLTP1-b and allele upLTP1-c had the highest rates in the landraces, and the dominant allele in the three peach wild relatives was allele upLTP1-b. Furthermore, we found many light-responsive elements in the upstream region. Most of the polymorphic sites in Prunus species are located in the intron region. Phylogenetic analysis suggests that Prunus kansuensis KC311794 and Prunus mira KC311791 are related more closely to Prunus persica KC311795 than to Prunus davidiana KC311792 and KC311793. The allele sequences we derived on the basis of this variability were distributed unevenly, indicating the need to study allergenicity in different subpopulations and the association between allele sequences and allergenicity.
Journal Article
Characteristics of depression, anxiety, impulsivity, and aggression among various types of drug users and factors for developing severe depression: a cross-sectional study
Background
Mood disorder, impulsivity and aggression are common in drug users compared to healthy controls. However, no study has focused on the difference in various types of drug users. Therefore, the objective of this study was to explore the differences in depression, anxiety, impulsivity, and aggression among methamphetamine, heroin and polysubstance users and to further explore the risk factors for severe depression in the three groups.
Methods
Drug users over 18 years old who met the DSM-V diagnostic criteria for substance -related disorders were included in the study. All participants completed a general questionnaire, the Zung Self-Rating Depression Scale (SDS), the Zung Self-Rating Anxiety Scale (SAS), Barratt impulsiveness Scale Version 11 (BIS-11), and the Buss-Perry Aggression Questionnaire (BPAQ). One-way ANOVAs or Chi-square tests were used to test the differences among the groups, correlation analysis was used to test the relationship between drug use and other parameters, and multiple logistic regression was conducted to assess the risk factors for severe depression.
Results
A total of 1,486 participants were included, comprising 86.3% males with a mean age of 38.97 years. There was a significant difference in the percentage of severe depression and SDS scores among the three groups, but no significant difference was found in SAS, BIS-11 and BPAQ scores. Using methamphetamines, hostility and anxiety were risk factors for developing severe depression in all the participants and anxiety remained constant in the other three groups. Moreover, methamphetamine use was 2.16 and 3.35 times more likely to cause severe depression than heroin and polysubstance use, respectively. The initial age of substance use was negatively correlated with BPAQ, SAS, and SDS scores, whereas the drug use duration and addiction duration were positively correlated.
Conclusions
In this study, we found that the highest prevalence of severe depression was in participants using methamphetamines and that using methamphetamines, hostility, and anxiety were risk factors for developing severe depression. This result addressed an important gap in our knowledge of the different characteristics of depression, anxiety, impulsivity and aggression in various types of substance users and provides clinicians and policy-makers with directions for intervention and preventing relapse.
Journal Article
The Experimental Investigation on the Internal Temperature Field of Regenerative Burners
The application of regenerative combustion technology to industry burners has been a huge success. This paper presents an experimental study on the change of the temperature field and the concentration of NO in the flue gas in an industrial furnace. The result showed that the concentration of NO increased with the rise of temperature. For the reversing time of 20s, 40s, 60s, and 80s, the temperature of each point changed in accordance with similar rules, but the temperature of the former three measuring points did not obviously change. The heat transfer occured mainly in the last two layers of the regenerative cells, and the utilization of all the regenerators was only 40 percent. The heating curve of the sixth measuring points was significantly different from the cooling one. With the increase of the reversing time, the flue gas and the air temperature variation also increased.
Journal Article
Gene regulation of anthocyanin biosynthesis in two blood-flesh peach （Prunus persica （L.） Batsch） cultivars during fruit development
The blood-flesh peach has become popular in China due to its attractive anthocyanin-induced pigmenta- tion and antioxidant properties. In this study, we investigated the molecular mechanisms underlying anthocyanin accumulation by examining the expression of nine genes of the anthocyanin biosynthesis pathway found in the peach mesocarp. Expression was measured at six developmental stages in fruit of two blood-flesh and one white-flesh peach cultivars, using quantitative reverse transcription polymerase chain reaction （qRT-PCR）. Results show that the expression of the chalcone synthase （CHS） gene was closely related to anthocyanin accumulation in both of the blood-flesh peaches. In the white-flesh peach, we found that the transcription level of phenylalanine ammonia-lyase （PAL） during fruit development was much lower than that in the blood-flesh peach, even though all other genes of the anthocyanin biosynthesis pathway were highly expressed, suggesting that the PAL gene may be limiting in antho- cyanin production in the white-flesh peach. Moreover, the transcription levels of the CHS and UDP-glucose-flavonoid 3-O-glucosyltransferase （UFGT） genes were markedly up-regulated at three days after bag removal （DABR） in the blood-flesh peach, suggesting that CHS and UFGT are the key genes in the process of anthocyanin biosynthesis for both of the blood-flesh peaches. The present study will be of great help in improving understanding of the molecular mechanisms involved in anthocyanin accumulation in blood-flesh peaches.
Journal Article