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Background Previous research has demonstrated a correlation between chronic stress and chronic pain (CP). However, there have been few studies examining the prospective association of allostatic load (AL)—the biological processes related to stress—with CP. Methods We firstly conducted latent class analysis to identify phenotypes of AL using a community-dwelling sample, the Midlife in the United States. Multinomial logistic regression models were used to examine the prospective association between phenotypes of AL at MIDUS 2 biomarker project and the presence of CP, CP interference and the number of CP sites at MIDUS 3. Results Three phenotypes of AL, low biological dysregulation, parasympathetic dysregulation and metabolic dysregulation, were identified. Compared to low biological dysregulation group, participants experiencing metabolic dysregulation phenotype of AL at MIDUS 2 had higher risks of having high-interference CP (RRR = 2.00, 95% CI: 1.06, 3.79, P  < 0.05) and 3 or more CP sites (RRR = 2.03, 95% CI: 1.08, 3.83, P  < 0.05) at MIDUS 3. Conclusion The findings indicate that focusing on mitigating the metabolic dysfunction phenotype of AL has the potential to be an efficacious strategy for alleviating future CP bodily widespreadness and high CP interference.

With the rapid development of sonodynamic therapy (SDT), sonosensitizers have evolved from traditional treatments to comprehensive diagnostics and therapies. Sonosensitizers play a crucial role in the integration of ultrasound imaging (USI), X-ray computed tomography (CT), and magnetic resonance imaging (MRI) diagnostics while also playing a therapeutic role. This review was based on recent articles on multifunctional sonosensitizers that were used in SDT for the treatment of cancer and have the potential for clinical USI, CT, and MRI applications. Next, some of the shortcomings of the clinical examination and the results of sonosensitizers in animal imaging were described. Finally, this paper attempted to inform the future development of sonosensitizers in the field of integrative diagnostics and therapeutics and to point out current problems and prospects for their application.

Cross-lingual summarization is the task of generating a summary in one language (e.g., English) for the given document(s) in a different language (e.g., Chinese). Under the globalization background, this task has attracted increasing attention of the computational linguistics community. Nevertheless, there still remains a lack of comprehensive review for this task. Therefore, we present the first systematic critical review on the datasets, approaches, and challenges in this field. Specifically, we carefully organize existing datasets and approaches according to different construction methods and solution paradigms, respectively. For each type of dataset or approach, we thoroughly introduce and summarize previous efforts and further compare them with each other to provide deeper analyses. In the end, we also discuss promising directions and offer our thoughts to facilitate future research. This survey is for both beginners and experts in cross-lingual summarization, and we hope it will serve as a starting point as well as a source of new ideas for researchers and engineers interested in this area.

Key messageHigh-resolution genome-wide association study (GWAS) facilitated QTL fine mapping and candidate gene identification, and the GWAS based genomic prediction models were highly predictive and valuable in wheat genomic breeding.Wheat is a major staple food crop and provides more than one-fifth of the daily calories and dietary proteins for humans. Genome-wide association study (GWAS) and genomic selection (GS) for wheat stress resistance and tolerance related traits are critical to understanding their genetic architecture for improvement of breeding selection efficiency. However, the insufficient marker density in previous studies limited the utility of GWAS and GS in wheat genomic breeding. Here, we conducted a high-resolution GWAS for wheat leaf rust (LR), yellow rust (YR), powdery mildew (PM), and cold tolerance (CT) by genotyping a panel of 768 wheat cultivars using genotyping-by-sequencing. Among 153 quantitative trait loci (QTLs) identified, 81 QTLs were delimited to ≤ 1.0 Mb intervals with three validated using bi-parental populations. Furthermore, 837 stress resistance-related genes were identified in the QTL regions with 12 showing induced expression by YR and PM pathogens. Genomic prediction using 2608, 4064, 3907, and 2136 pre-selected SNPs based on GWAS and genotypic correlations between the SNPs showed high prediction accuracies of 0.76, 0.73, and 0.78 for resistance to LR, YR, and PM, respectively, and 0.83 for resistance to cold damage. Our study laid a solid foundation for large-scale QTL fine mapping, candidate gene validation and GS in wheat.

In order to solve the problems of low hardness and poor wear resistance of titanium alloy, laser cladding technology was used to prepare Ti(C,B)/Ni coatings with different amount of Cr3C2 on the surface of Ti-6Al-4V titanium alloy. The effects of Cr3C2 content on the microstructure, hardness and wear resistance of Ti(C,B)/Ni coating were investigated by methods of scanning electron microscope (SEM), x-ray diffractometer (XRD), microhardness tester and M-200 wear tester. The results showed that phases existing in coating mainly contain γ-Ni, Ni3Ti, NiTi, TiB2, Ti(C,B) and CrB. With the increase of Cr3C2 content, the size and number of black bulk precipitates in the coating reduced. Meanwhile, the short rod-like Ti(C,B) particles grew and the amount of the particles increased, and Ti(C,B) particles existed in the form of cellular dendritic crystals in the coating. The average microhardness of the cladding layer without Cr3C2 addition is 896.7 HV0.2, which is about 1.5 times higher than that of Ti-6Al-4V substrate (360 HV0.2). With the increase of Cr3C2 content (10, 20, 30 wt.%), the average microhardness of the coating increased to 948.7, 990.4 and 1035.8 HV0.2, respectively. The average hardness of the coating added 30 wt.%Cr3C2 is 15.5% higher than that of the Cr3C2-free coating. At the same time, the wear resistance increased with the increase of Cr3C2 content.

Carbidic austempered ductile iron (CADI) developed in the United States is a new wear-resistant material, which is widely used in general wear parts, machinery, equipment, bearing components, gears and grinding balls in mining ball mills. It has some advantages of its light weight, good toughness and shock absorption, excellent wear resistance, low cost. However, the research of CADI is still in the early stage, and timely updates of recent research progress on new remarkable breakthroughs in CADI are highly desired. The alloy design and the production method of CADI were summarized in the present review. In addition, the future directions were discussed. We hope our work could guide the following development and research of CADI.

Carcinoembryonic antigen–related cell adhesion molecule 5 (CEACAM-5) assays are employed in routine clinical settings to diagnose tumor. We selected two nanobodies with high-affinity to CEACAM-5, termed Nb11C12 and Nb2D5, using phage-display technology. The Nb2D5 fused with calf intestinal alkaline phosphatase (CAP), human placental alkaline phosphatase (HAP), or Pyrococcus abyssi alkaline phosphatase (PAP) were expressed in human embryonic kidney (HEK293) cells. The enzymatic activity of Nb2D5-HAP fusion protein was the best and remained stable at 60 °C for 7 days. The affinity of Nb2D5-HAP fusion protein to CEACAM-5 reached 42 pM. A chemiluminescent enzyme immunoassay (CLEIA) based on Nb2D5-HAP fusion protein was established for quantitative CEACAM-5 assay in clinical settings. The CLEIA exhibited a wide linear range of 0.31–640 ng/mL toward CEACAM-5, with a limit of detection (LOD) of 0.85 ng/mL. No cross-reactivity occurred with CEACAM-1, CEACAM-3, CEACAM-6, or CEACAM-8, and no interference was observed with rheumatoid factors. The CLEIA based on Nb2D5-HAP fusion protein was stable for 8 weeks at 37 °C and 50% relative humidity. The CLEIA developed from Nb2D5-HAP fusion protein had much better stability and linearity with similar reproducibility compared with the enzyme-linked immunosorbent assay developed from conventional monoclonal antibodies, which have been widely used in clinics over the past several decades.

Ethyl methanesulfonate (EMS)-induced mutagenesis is a prominent method for generating plant mutants, often resulting in chimera plants; however, their transcriptional and genetic characteristic remain elusive. In this investigation, chimera pea ( Pisum sativum L.) specimens, labeled GY1 and GY2, exhibiting a distinctive phenotype with yellow and green leaves were meticulously cultivated via sequential double EMS mutagenesis. The observed color disparity between the yellow and green leaves was attributed to a significant reduction in chlorophyll content coupled with heightened lutein levels in both chimeric variants. Transcriptome profiling revealed the enrichment of differentially expressed genes in both GY1 and GY2, specifically implicating Kyoto Encyclopedia of Genes and Genomes pathways linked to amino acid biosynthesis and ribosome development, alongside Gene Ontology (GO) biological processes linked with stress response mechanisms. Few structural genes associated with chlorophyll and lutein biosynthesis exhibited discernible differential expression. Despite these functional similarities, distinctive nuances were evident between specimens, with GY1 exhibiting enrichment in GO pathways related to chloroplast development and GY2 showing enrichment for ribosome development pathways. Single-nucleotide polymorphism (SNP) analysis uncovered a shared pool of 599 and 598 polymorphisms in the yellow and green leaves of GY1 and GY2, respectively, likely stemming from the initial EMS mutagenesis step. Further investigation revealed an increased number of unique SNPs in the yellow leaves following the second EMS application, whereas the green leaves exhibited sparse and unique SNP occurrences, suggestive of potential evasion from secondary mutagenesis. This inherent genetic variability underpins the mechanism underlying the formation of chimera plants. Predominant base mutations induced by EMS were characterized by G/A and C/T transitions, constituting 74.1% of the total mutations, aligning with established EMS mutation induction paradigms. Notably, genes encoding the eukaryotic translation initiation factor eIIso4G and the ubiquitin ligase RKP, known to modulate leaf color in model plants, harbored two SNPs in the yellow leaves of both GY1 and GY2, implicating their putative role in the yellow leaf phenotype. Collectively, this study provides novel insights into the transcriptional and genetic characteristics of chimera plants via EMS-induced mutagenesis.

The rapid development of coastal cities has intensified land resource constraints and is leading to an increasing number of foundation pit projects near existing operational tunnels. This necessitates careful consideration of coastal excavation impacts on adjacent tunnels. Taking a foundation pit project in Qingdao as a case study, this paper investigates tunnel deformation through statistical analysis, numerical simulation, and field monitoring. By adjusting numerical model parameters, the research examines the influence of horizontal clearance distances, existing structure burial depths, and different retaining structure configurations on tunnel deformation, providing guidance for deformation control. Key findings include the following: (1) Statistical analysis reveals that tunnels in silty clay strata experience more significant excavation-induced deformation compared to those in silt strata, with relative positional relationships between pits and tunnels playing a critical role. (2) Numerical and monitoring results demonstrate that pit excavation induces tunnel displacement towards the excavation zone. Maximum lateral displacement reached 3.57 mm (simulated) and 4.79 mm (measured), while maximum vertical displacement was 3.11 mm (simulated) and 3.85 mm (measured), all within safety thresholds. (3) Sensitivity analysis shows that shallower tunnels exhibit more pronounced deformations. Increasing horizontal separation distance from 10 m to 25 m reduces deformation by one-third. However, adjusting diaphragm wall thickness and retaining structure embedment depth proves limited in deformation control, necessitating reinforcement measures on the tunnel side. These findings provide valuable references for protecting coastal silty clay stratum tunnels.