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Human organoid systems recapitulate key features of organs offering platforms for modelling developmental biology and disease. Tissue-derived organoids have been widely used to study the impact of extrinsic niche factors on stem cells. However, they are rarely used to study endogenous gene function due to the lack of efficient gene manipulation tools. Previously, we established a human foetal lung organoid system (Nikolić et al., 2017). Here, using this organoid system as an example, we have systematically developed and optimised a complete genetic toolbox for use in tissue-derived organoids. This includes ‘Organoid Easytag’, our efficient workflow for targeting all types of gene loci through CRISPR-mediated homologous recombination followed by flow cytometry for enriching correctly targeted cells. Our toolbox also incorporates conditional gene knockdown or overexpression using tightly inducible CRISPR interference and CRISPR activation which is the first efficient application of these techniques to tissue-derived organoids. These tools will facilitate gene perturbation studies in tissue-derived organoids facilitating human disease modelling and providing a functional counterpart to many ongoing descriptive studies, such as the Human Cell Atlas Project.

As part of establishing a management system to prevent the illegal transfer of nuclear items, automatic nuclear item detection technology is required during customs clearance. However, it is challenging to acquire X-ray images of major nuclear items (e.g., nuclear fuel and gas centrifuges) loaded in cargo with which to train a cargo inspection model. In this work, we propose a new means of data augmentation to alleviate the lack of X-ray training data. The proposed augmentation method generates synthetic X-ray images for the training of semantic segmentation models combining the X-ray images of nuclear items and X-ray cargo background images. To evaluate the effectiveness of the proposed data augmentation technique, we trained representative semantic segmentation models and performed extensive experiments to assess its quantitative and qualitative performance capabilities. Our findings show that multiple item insertions to respond to actual X-ray cargo inspection situations and the resulting occlusion expressions significantly affect the performance of the segmentation models. We believe that this augmentation research will enhance automatic cargo inspections to prevent the illegal transfer of nuclear items at airports and ports.

Image steganalysis is the task of detecting a secret message hidden in an image. Deep steganalysis using end-to-end deep learning has been successful in recent years, but previous studies focused on improving detection performance rather than designing a lightweight model for practical applications. This caused a deep steganalysis model to be heavy and computationally costly, making the model infeasible to deploy in real-world applications. To address this issue, we study an effective model design strategy for lightweight image steganalysis. Considering the domain-specific characteristics of steganalysis, we propose a simple yet effective block removal strategy that progressively removes a sequence of blocks from deep classification networks. This method involves the gradual removal of convolutional neural network blocks, starting from deeper ones. By doing so, the number of parameters and FLOPs are decreased without compromising the detection performance. Experimental results show that our removal strategy makes the EfficientNet-B0 variants 9.58 × smaller and has 2.16 × fewer FLOPs than the baseline while retaining detection accuracy of 90.73% and 82.40% that are on par with the baseline on BOSSBase and ALASKA#2 datasets, respectively. Backed by our in-depth analyses, the results indicate that only a few early layers are sufficient for effective image steganalysis.

Lymphangioleiomyomatosis (LAM) is a rare disease of women in which TSC2 deficient ‘LAM cells’ with dysregulated mTOR signalling and recruited fibroblasts form nodules causing lung cysts and respiratory failure. We examine if mTOR dysregulation can induce senescence and impair the response to lung injury in LAM. The senescence markers p21, p16 and the SenMayo gene set are increased in LAM lungs and colocalise with alveolar type 2 cells. LAM models induce mTOR dependent senescence in alveolar type 2 cell organoids in vitro and in vivo. IL-6 produced by LAM cells, induces p16 and p21 in alveolar type 2 cells, inhibits epithelial wound resolution and is related to lung function in LAM patients. Rapamycin and the IL-6 receptor antagonist Tocilizumab reduce alveolar type 2 cell organoid p21 accumulation and Tocilizumab enhances epithelial wound repair. Targeting IL-6 signalling in parallel with mTOR inhibition, may reduce lung damage in LAM. Lymphangioleiomyomatosis (LAM) is a rare disease in women where TSC2 deficient mTOR signalling aberrant LAM cells and fibroblasts form nodules causing lung cysts and respiratory failure. Here the authors examine how mTOR dependent IL-6 causes senescence in alveolar type 2 cells which may result in impaired lung repair.

Although Korean language education is experiencing rapid growth in recent years and several studies have investigated automated writing evaluation (AWE) systems, AWE for Korean L2 writing still remains unexplored. Therefore, this study aims to develop and validate a state-of-the-art neural model AWE system which can be widely used for Korean language teaching and learning. Based on a Korean learner corpus, the proposed AWE is developed using natural language processing techniques such as part-of-speech tagging, syntactic parsing, and statistical language modeling to engineer linguistic features and a pre-trained neural language model. This study attempted to determine how neural network models use different linguistic features to improve AWE performance. Experimental results of the proposed AWE tool showed that the neural AWE system achieves high reliability for unseen test data from the corpus, which implies metrics used in the AWE system can help differentiate different proficiency levels and predict holistic scores. Furthermore, the results confirmed that the proposed linguistic features–syntactic complexity, quantitative complexity, and fluency–offer benefits that complement neural automated writing evaluation.

In this paper, we propose a novel way of improving named entity recognition (NER) in the Korean language using its language-specific features. While the field of NER has been studied extensively in recent years, the mechanism of efficiently recognizing named entities (NEs) in Korean has hardly been explored. This is because the Korean language has distinct linguistic properties that present challenges for modeling. Therefore, an annotation scheme for Korean corpora by adopting the CoNLL-U format, which decomposes Korean words into morphemes and reduces the ambiguity of NEs in the original segmentation that may contain functional morphemes such as postpositions and particles, is proposed herein. We investigate how the NE tags are best represented in this morpheme-based scheme and implement an algorithm to convert word-based and syllable-based Korean corpora with NEs into the proposed morpheme-based format. Analyses of the results of traditional and neural models reveal that the proposed morpheme-based format is feasible, and the varied performances of the models under the influence of various additional language-specific features are demonstrated. Extrinsic conditions were also considered to observe the variance of the performances of the proposed models, given different types of data, including the original segmentation and different types of tagging formats.

In the paper, we propose a novel way of improving named entity recognition in the Korean language using its language-specific features. While the field of named entity recognition has been studied extensively in recent years, the mechanism of efficiently recognizing named entities in Korean has hardly been explored. This is because the Korean language has distinct linguistic properties that prevent models from achieving their best performances. Therefore, an annotation scheme for {Korean corpora} by adopting the CoNLL-U format, which decomposes Korean words into morphemes and reduces the ambiguity of named entities in the original segmentation that may contain functional morphemes such as postpositions and particles, is proposed herein. We investigate how the named entity tags are best represented in this morpheme-based scheme and implement an algorithm to convert word-based {and syllable-based Korean corpora} with named entities into the proposed morpheme-based format. Analyses of the results of {statistical and neural} models reveal that the proposed morpheme-based format is feasible, and the {varied} performances of the models under the influence of various additional language-specific features are demonstrated. Extrinsic conditions were also considered to observe the variance of the performances of the proposed models, given different types of data, including the original segmentation and different types of tagging formats.

The current research direction in generative models, such as the recently developed GPT4, aims to find relevant knowledge information for multimodal and multilingual inputs to provide answers. Under these research circumstances, the demand for multilingual evaluation of visual question answering (VQA) tasks, a representative task of multimodal systems, has increased. Accordingly, we propose a bilingual outside-knowledge VQA (BOK-VQA) dataset in this study that can be extended to multilingualism. The proposed data include 17K images, 17K question-answer pairs for both Korean and English and 280K instances of knowledge information related to question-answer content. We also present a framework that can effectively inject knowledge information into a VQA system by pretraining the knowledge information of BOK-VQA data in the form of graph embeddings. Finally, through in-depth analysis, we demonstrated the actual effect of the knowledge information contained in the constructed training data on VQA.

The impressive development of large language models (LLMs) is expanding into the realm of large multimodal models (LMMs), which incorporate multiple types of data beyond text. However, the nature of multimodal models leads to significant expenses in the creation of training data. Furthermore, constructing multilingual data for LMMs presents its own set of challenges due to language diversity and complexity. Therefore, in this study, we propose two cost-effective methods to solve this problem: (1) vocabulary expansion and pretraining of multilingual LLM for specific languages, and (2) automatic and elaborate construction of multimodal datasets using GPT4-V. Based on015 these methods, we constructed a 91K English-Korean-Chinese multilingual, multimodal training dataset. Additionally, we developed a bilingual multimodal model that exhibits excellent performance in both Korean and English, surpassing existing approaches.