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Diclofenac sodium, as a typical deputation of non-steroidal anti-inflammatory drugs, is widely used in clinical treatment. Due to the heavy use, diclofenac sodium is commonly detected in water environment, and the removal of diclofenac sodium from wastewater is important for environmental protection. Notably, magnetic separation technology has become a novel performance in the removal of organic pollutants from wastewater in recent years. Herein, we engineered a lignin-based magnetic nanoparticle adsorbent (LMNA) by loading a magnetic core (Fe3O4) onto alkali lignin. This novel adsorbent has the advantage of green synthesis and low cost, making it an ideal material for wastewater treatment. Remarkably, the BET surface area of LMNA (739.2 m2 g−1) was higher than that of alkali lignin (2.2 m2 g−1). Adsorption batch experiments confirmed that the LMNA exhibited good adsorption performance to diclofenac sodium with a higher adsorption capacity of 106.4 mg g−1. The adsorption kinetic data and isothermal were well fitted by the pseudo-second-order rate equation (R2 = 0.980) and the Langmuir equation (R2 = 0.991), respectively. Moreover, the reaction mechanisms between the diclofenac sodium and LMNA mainly related to the synergism of electrostatic attractions, π–π stacking interactions and hydrogen bonding interactions. Especially, the LMNA exhibited high magnetic saturation strength (10.5 emu g−1) which made it easy to recycle, showing excellent reusability (4 cycles). Our work might introduce significant theoretical and experimental basis for realizing excellent adsorption of emerging organic pollutants from wastewater by the magnetic nanoparticle adsorbent.Graphic Abstract

Proteins and polyphenols are important components in barley malt. During the roasting process of barley malt, proteins and polyphenols interact and influence each other, ultimately altering the nutritional profile and functional properties of barley malt. In this research, polyphenol-free proteins and protein–polyphenol complexes were extracted from barley malt subjected to varying degrees of roasting. The antioxidant activity of protein–polyphenol complexes was assessed by ABTS, FRAP, and ORAC assays. The structural characteristics of the proteins were examined through UV, FL, CD, FTIR, and SEM. We found that roasting enhances the solubility of globulin, prolamin, and glutenin and facilitates the binding of these proteins with polyphenols. Conversely, the impact of roasting on albumin exhibits a trend opposite to that observed in the other three proteins. The antioxidant activity of protein–polyphenol complexes was significantly higher than that of polyphenol-free proteins. Additionally, the microenvironment of the amino acid residues of the four proteins exhibited increased polarity following the roasting process, and the structural conformation of albumin, globulin, and glutelin transitioned from an ordered to a disordered state. Our results indicate that roasting enhances the antioxidant activity of protein–polyphenol complexes by altering the secondary and tertiary structures of these proteins, thereby exposing more hydrophobic side-chain groups inside the proteins and offering more binding sites for polyphenols.

In order to regulate turbulence strength and determine airflow characteristics in a new dual-feed rotor spinning unit, the internal flow field is investigated. A computational fluid dynamics technique is employed to numerically study the three-dimensional model of the internal airflow in the new design. The effects of air velocity variation on turbulence strength, negative pressure, Re, and wall pressure distribution are investigated based on simulation data and previous studies. The results show that the turbulence strength and Re increased with increase in inlet air velocity. Pressure profiles inside the rotor varied significantly with positive pressure observed at the channel exits. Minimal inlet velocity maintains the flow field in the rotor interior below 100 m/s, which gives the ideal turbulence required to minimize yarn quality deterioration. The dual-feed rotor spinning unit showed more orderly streamline patterns with fewer vortices compared to the conventional one. The numerical simulation can provide insights on airflow studies and some guidelines for future prototyping and experiments to further improve the new design.

Background Previous research on the lower gastrointestinal tract has proved that microbial dysbiosis can lead to intestinal barrier dysfunction and enhanced visceral sensitivity, thus triggering bowel symptoms. Whether esophageal microbial dysbiosis also contributes to the development of gastroesophageal reflux (GER) symptoms, which are known to be associated with impaired esophageal barrier integrity, remains to be explored. Methods Patients with GER symptoms (gastroesophageal reflux disease [GERD] and functional esophageal disorders [FED]), duodenal ulcer patients and healthy controls were prospectively included for esophageal microbial analysis. The expression of toll-like receptors (TLRs) and tight junction proteins and intercellular spaces were assessed through transcriptome analysis and immunohistochemistry. The human esophageal epithelial cell (HEEC) line was used to explore how esophageal microbial dysbiosis induced GER symptoms. Results Patients with GER symptoms, whether GERD or FED, had a very similar pattern of microbial composition, which showed a significantly increased proportion of Gram-negative bacteria than controls. Patients with GER symptoms (GERD and FED) also exhibited significantly higher TLR2 expression, reduced claudin-1 expression and dilated intercellular spaces (DIS). In vitro, exposure of HEECs to lipopolysaccharide resulted in marked up-regulation of TLR2 and interleukin (IL)-6, down-regulation of claudin-1 and DIS. These effects were mitigated by blocking TLR2 or IL-6. Conclusion This study demonstrated that regardless of objective evidence of reflux, patients with GER symptoms presented esophageal microbial dysbiosis characterized by an elevated proportion of Gram-negative bacteria. Enriched Gram-negative bacteria could induce esophageal barrier dysfunction via LPS-TLR2-IL-6-claudin-1-DIS pathway.

The current study was aimed to explore the beneficial effects of polysaccharides isolated from Laminaria japonica (LP) on gestational diabetes mellitus (GDM) mice. The obtained results demonstrated that the LP improved serum biochemical index, body weight index, and glucose tolerance. Furthermore, the evidence also suggested that the beneficial effects of LP might be attributed to the alternation in gut microbiota by LP supplementation. Particularly, the Turicibacter, a short‐chain fatty acids‐producing bacterium, was found to be up‐regulated. In conclusion, the obtained results indicated that the LP might serve as prebiotic, and might have a great potential to be used as food supplementation for GDM patients. Laminaria japonica polysaccharides (LP) improved serum biochemical index, body weight index, and glucose tolerance in gestational diabetes mellitus (GDM) mice. This beneficial effects of LP could be associated with the alternation in gut microbiota. Particularly, the Turicibacter, a short‐chain fatty acids‐producing bacterium, was found to be up‐regulated by LP supplementation.

Ischemic stroke (IS), as a leading cause of disability worldwide, affects intestinal bacterial communities and their metabolites, while recent discoveries have highlighted the importance of the intestinal microflora in the development of IS. Systematic investigations of complex intestinal bacterial communities and their metabolites during ischemic brain injury contribute to elucidate the promising therapeutic targets for IS. However, the associations between intestinal microbiota and related circulating metabolic processes in IS remained unclear. Hence, to identify the changed microflora and their metabolites in IS of NaoMaiTong (NMT), an effective clinical medication, we established the middle cerebral artery occlusion/reperfusion (MCAO/R) model using conventionalized and pseudo-germ-free (PGF) rats. Subsequently, we systematically screen the microflora and related metabolites changing in IS via an integrated approach of cecal 16S rRNA sequencing combined with plasma metabolomics. We found that NMT relied on intestinal flora to improve stroke outcome in conventionalized rats while the protection of NMT was reduced in PGF rats. Total 35 differential bacterial genera and 26 differential microbial metabolites were regulated by NMT. Furthermore, L-asparagine and indoleacetaldehyde were significantly negatively correlated with Lachnospiraceae_UCG.001 and significantly positively correlated with Lachnoclostridium . Indoleacetaldehyde also presented a negative correlation with Lactobacillus and Bifidobacterium . 2-Hydroxybutyric acid was strongly negatively correlated with Ruminococcus, Lachnospiraceae_UCG.001 and Lachnospiraceae_UCG.006. Creatinine was strongly negatively correlated with Akkermansia . In summary, the research provided insights into the intricate interaction between intestinal microbiota and metabolism of NMT in IS. We identified above differential bacteria and differential endogenous metabolites which could be as prebiotic and probiotic substances that can influence prognosis in stroke and have potential to be used as novel therapeutic targets or exogenous drug supplements.

As one of the most technically and managerially complex types of construction projects, super-high-rise buildings require deep multidisciplinary integration and intensive collaboration throughout their lifecycle. Conventional stage-based delivery models, such as the EPC, are often inadequate for handling this complexity. In recent years, the integrated Financing–Engineering, Procurement and Construction–Operation (F+EPC+O) model has emerged to address lifecycle governance challenges in building projects. This study explores how an investment-led F+EPC+O model builds dynamic capabilities to enable lifecycle collaboration in complex projects. It is based on a case study of the Xiamen Hemei Center and employs a qualitative case study approach to examine the operation of an internal F+EPC+O in the project. Drawing on multi-source data, including internal archives, BIM/CIM logs, and interviews, the findings identify three elements—lifecycle incentive alignment, internal power symmetry, and extended operation duration—that shape the Sensing–Seizing–Reconfiguring (SSR) capabilities of the approach. Specifically, Sensing is achieved through NPV-based decision frameworks and cross-stage trade-off lists; Seizing is achieved through BIM/CIM issue closure and joint rapid-cycle decision-making; and Reconfiguring is achieved through performance feedback and institutionalized knowledge repositories. The findings indicate that the SSR dynamic cycle transforms institutional integration into value co-creation, turning project complexity into a source of collaborative advantage.

The ALOG (Arabidopsis LIGHT-DEPENDENT SHORT HYPOCOTYLS 1 (LSH1) and Oryza G1) family play crucial regulatory roles in plant growth and development, spanning both vegetative and reproductive growth. This study presents a comprehensive genomic and functional analysis of the ALOG family in greater yam (Dioscorea alata). Ten non-redundant DaALOG genes were identified and classified into two classes (I and II) based on phylogenetic analysis. These classes share a common origin, and family expansion was primarily driven by segmental duplication events. Comparative genomics across 15 plant species revealed widespread, lineage-specific divergence in ALOG gene family size and composition. Expression profiling highlighted several DaALOG genes, particularly DaALOG1, DaALOG3, and DaALOG6A, with significant upregulation in tuber and bulbil tissues, suggesting a potential role in storage organ development. Co-expression network analysis, coupled with yeast one-hybrid assays, indicated that DaALOG3 likely regulates key genes involved in starch biosynthesis. Subcellular localization confirmed the nuclear predominance of DaALOG proteins. Furthermore, functional validation in Arabidopsis demonstrated that overexpression of DaALOG1 leads to pronounced developmental alterations, including irregular leaf morphology and floral organ abnormalities (such as extra stamens and petals). Collectively, our findings establish the DaALOG gene family as an important regulator in greater yam, linking specific members to both vegetative architecture and storage organ development.

Purpose The effectiveness of anastrozole for breast cancer prevention has been demonstrated. The objective of this study was to evaluate the cost-effectiveness of anastrozole for the prevention of breast cancer in women with a high risk of breast cancer and to determine whether anastrozole for the primary prevention of breast cancer can improve the quality of life of women and save health-care resources. Methods A decision-analytic model was used to assess the costs and effects of anastrozole prevention versus no prevention among women with a high risk of breast cancer. The key parameters of probability were derived from the IBIS-II trial, and the cost and health outcome data were derived from published literature. Costs, quality-adjusted life-years (QALYs), and incremental cost-effectiveness ratios (ICERs) were calculated for the two strategies,One-way and probabilistic sensitivity analyses were performed. Results In the base case, the incremental cost per QALY of anastrozole prevention was £125,705.38/QALY in the first 5 years compared with no prevention in the UK, above the threshold of WTP (£3,000/QALY),and in the 12-year period, the ICER was £8,313.45/QALY, less than WTP. For the US third-party payer, ICER was $134,232.13/QALY in the first 5 years and $8,843.30/QALY in the 12 years, both less than the WTP threshold ($150,000/QALY). Conclusion In the UK and US, anastrozole may be a cost-effective strategy for the prevention of breast cancer in high-risk postmenopausal women. Moreover, the longer the cycle of the model, the higher the acceptability. The results of this study may provide a scientific reference for decision-making for clinicians, patients, and national medical and health care government departments.

The conventional rotor spinning unit generates flow vortices in the transfer channel upstream region which affect the fiber configuration and consequently yarn properties. Geometry and spinning parameters such as transfer channel length, inlet width, rotor outlet pressure, opening roller speed, and diameter were found to be key parameters influencing airflow characteristics. To reduce the flow vortices in the upper stream region, modifications of the transfer channel were proposed, and their airflow fields were analyzed using computational fluid dynamics. Three designs were studied: a round transfer channel inlet, a bypass channel for extra air supply, and one with both the bypass and the round inlet. Analysis of airflow revealed that the design with both round transfer channel inlet and a bypass proved to be very effective in properly directing the flow and minimizing vortices. The design was also characterized by smoother velocity streamlines and maximum mass flow across the transfer channel. A conventional rotor spinning unit was modified in which a round transfer channel inlet corner and a bypass channel were utilized to conduct the experimental tests. Three sets of yarn samples were produced using the conventional and modified rotor spinning units under different rotor speed conditions. Yarn properties were tested. Properties such as tenacity, CVm%, and thin and thick places of the spun yarns produced by the new design improved compared to that of the conventional yarn.