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Satellite products have mediocre performance in precipitation estimation, while rain gauges are incapable of describing continuous spatial precipitation distributions. To obtain spatially continuous and accurate precipitation data, this paper proposes a two-step scheme incorporating environmental variables, satellite precipitation estimations, and rain gauge observations for the calibration of satellite precipitation data. First, the GPM data are downscaled from 0.1° to 0.01° based on the seasonal RF models to minimize the spatial differences between the satellite estimations and the rain gauge observations. Secondly, the fusion model combining ConvLSTM and CBAM explores the spatiotemporal correlation of downscaled satellite precipitation data with environmental co-variables and ground-based observations to correct GPM precipitation. The integrated scheme (CBAM-ConvLSTM) is applied to acquire monthly precipitation at a spatial resolution of 0.01° over Hanjiang River Basin from 2014 to 2018. Comparative analyses of model-based satellite products with in situ observations show that model-based precipitation products have a high-resolution spatial distribution along with high accuracy, which combines the advantages of in situ observations and satellite products. Compared to the original GPM product, the evaluation metric values of the merged precipitation products all improved: the RMSE decreased by 31% while the CC increased from 0.55 to 0.69, the bias decreased from about 25% to less than 1.8%, and the MAE decreased by 27.8% while the KGE increased from 0.28 to 0.52. This two-step scheme provides an effective way to derive a high-resolution and accurate monthly precipitation product for humid regions.

To improve the accuracy and reliability of precipitation estimation, numerous models based on machine learning technology have been developed for integrating data from multiple sources. However, little attention has been paid to extracting the spatiotemporal correlation patterns between satellite products and rain gauge observations during the merging process. This paper focuses on this issue by proposing an integrated framework to generate an accurate and reliable spatiotemporal estimation of precipitation. The proposed framework integrates Funk-Singular Value Decomposition (F-SVD) in the recommender system to achieve the accurate spatial distribution of precipitation based on the spatiotemporal interpolation of rain gauge observations and Convolutional Long Short-Term Memory (ConvLSTM) to merge precipitation data from interpolation results and satellite observation through exploiting the spatiotemporal correlation pattern between them. The framework (FS-ConvLSTM) is utilized to obtain hourly precipitation merging data with a resolution of 0.1° in Jianxi Basin, southeast of China, from both rain gauge data and Global Precipitation Measurement (GPM) from 2006 to 2018. The LSTM and Inverse Distance Weighting (IDW) are constructed for comparison purposes. The results demonstrate that the framework could not only provide more accurate precipitation distribution but also achieve better stability and reliability. Compared with other models, it performs better in variation process description and rainfall capture capability, and the root mean square error (RSME) and probability of detection (POD) are improved by 63.6% and 22.9% from the original GPM, respectively. In addition, the merged precipitation combines the strength of different data while mitigating their weaknesses and has good agreement with observed precipitation in terms of magnitude and spatial distribution. Consequently, the proposed framework provides a valuable tool to improve the accuracy of precipitation estimation, which can have important implications for water resource management and natural disaster preparedness.

The Temporal Convolutional Network (TCN) and TCN combined with the Encoder-Decoder architecture (TCN-ED) are proposed to forecast runoff in this study. Both models are trained and tested using the hourly data in the Jianxi basin, China. The results indicate that the forecast horizon has a great impact on the forecast ability, and the concentration time of the basin is a critical threshold to the effective forecast horizon for both models. Both models perform poorly in the low flow and well in the medium and high flow at most forecast horizons, while it is subject to the forecast horizon in forecasting peak flow. TCN-ED has better performance than TCN in runoff forecasting, with higher accuracy, better stability, and insensitivity to fluctuations in the rainfall process. Therefore, TCN-ED is an effective deep learning solution in runoff forecasting within an appropriate forecast horizon.

The prevalent DNA modification in higher organisms is the methylation of cytosine to 5-methylcytosine (5mC), which is partially converted to 5-hydroxymethylcytosine (5hmC) by the Tet (ten eleven translocation) family of dioxygenases. Despite their importance in epigenetic regulation, it is unclear how these cytosine modifications are reversed. Here, we demonstrate that 5mC and 5hmC in DNA are oxidized to 5-carboxylcytosine (5caC) by Tet dioxygenases in vitro and in cultured cells. 5caC is specifically recognized and excised by thymine-DNA glycosylase (TDG). Depletion of TDG in mouse embyronic stem cells leads to accumulation of 5caC to a readily detectable level. These data suggest that oxidation of 5mC by Tet proteins followed by TDG-mediated base excision of 5caC constitutes a pathway for active DNA demethylation.

Background ( R )-[3,5-bis(trifluoromethyl)phenyl] ethanol [( R )-3,5-BTPE] is a valuable chiral intermediate for Aprepitant (Emend) and Fosaprepitant (Ivemend). Biocatalyzed asymmetric reduction is a preferred approach to synthesize highly optically active ( R )-3,5-BTPE. However, the product concentration and productivity of reported ( R )-3,5-BTPE synthetic processes remain unsatisfied. Results A NADPH-dependent carbonyl reductase from Lactobacillus kefir ( Lk CR) was discovered by genome mining for reduction of 3,5-bis(trifluoromethyl) acetophenone (3,5-BTAP) into ( R )-3,5-BTPE with excellent enantioselectivity. In order to synthesize ( R )-3,5-BTPE efficiently, Lk CR was coexpressed with glucose dehydrogenase from Bacillus subtilis ( Bs GDH) for NADPH regeneration in Escherichia coli BL21 (DE3) cells, and the optimal recombinant strain produced 250.3 g/L ( R )-3,5-BTPE with 99.9% ee but an unsatisfied productivity of 5.21 g/(L h). Then, four different linker peptides were used for the fusion expression of Lk CR and Bs GDH in E. coli to regulate catalytic efficiency of the enzymes and improved NADPH-recycling efficiency. Using the best strain ( E. coli /pET- Bs GDH-ER/K(10 nm)- Lk CR), up to 297.3 g/L ( R )-3,5-BTPE with enantiopurity >99.9% ee was produced via reduction of as much as 1.2 M of substrate with a 96.7% yield and productivity of 29.7 g/(L h). Conclusions Recombinant E. coli /pET- Bs GDH-ER/K(10 nm)- Lk CR was developed for the bioreduction of 3,5-BTAP to ( R )-3,5-BTPE, offered the best results in terms of high product concentration and productivity, demonstrating its great potential in industrial manufacturing of ( R )-3,5-BTPE.

Background: A multiferroic material can simultaneously show two or more basic magnetic properties, including ferromagnetism, antiferromagnetism, and ferroelectricity. BiFeO3 is a multiferroic material with a rhombohedral distorted perovskite structure. Doping can reduce the volatility of Bi and greatly improve the magnetoelectric properties of BiFeO3. Methods: To investigate the influence of the doping content we used the following analytical methods: X-ray powder diffraction (XRD), scanning electron microscopy (SEM), microwave network analysis (PNA-N5244A), and the Superconducting Quantum Interference Device (Quantum Design MPMS) test. Results: With the increase of Ca2+ concentration in the solution, the grain size of Bi1-xCa x FeO3 becomes smaller, showing the role of Ca2+ ions as the dopant for fine grains. The calcination temperatures are the major causes for the saturated magnetization. The residual magnetization (Mr) and the coercive force (Hc) decrease linearly with the increase of x value, and due to the effect of Ca2+ substitution at Bi3+ sites, which causes the valence change of Fe and/or the oxygen vacancies. Conclusions: The XRD result indicates that the diffraction peak emerges with the increase of Ca2+ and the main diffraction peak achieves a high angle. The best calcining temperature is 600 °C, and the morphology is very dependent on the calcining temperature.

(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol [(R)-3,5-BTPE] is a valuable chiral intermediate for Aprepitant (Emend) and Fosaprepitant (Ivemend). Biocatalyzed asymmetric reduction is a preferred approach to synthesize highly optically active (R)-3,5-BTPE. However, the product concentration and productivity of reported (R)-3,5-BTPE synthetic processes remain unsatisfied. A NADPH-dependent carbonyl reductase from Lactobacillus kefir (LkCR) was discovered by genome mining for reduction of 3,5-bis(trifluoromethyl) acetophenone (3,5-BTAP) into (R)-3,5-BTPE with excellent enantioselectivity. In order to synthesize (R)-3,5-BTPE efficiently, LkCR was coexpressed with glucose dehydrogenase from Bacillus subtilis (BsGDH) for NADPH regeneration in Escherichia coli BL21 (DE3) cells, and the optimal recombinant strain produced 250.3 g/L (R)-3,5-BTPE with 99.9% ee but an unsatisfied productivity of 5.21 g/(L h). Then, four different linker peptides were used for the fusion expression of LkCR and BsGDH in E. coli to regulate catalytic efficiency of the enzymes and improved NADPH-recycling efficiency. Using the best strain (E. coli/pET-BsGDH-ER/K(10 nm)-LkCR), up to 297.3 g/L (R)-3,5-BTPE with enantiopurity >99.9% ee was produced via reduction of as much as 1.2 M of substrate with a 96.7% yield and productivity of 29.7 g/(L h). Recombinant E. coli/pET-BsGDH-ER/K(10 nm)-LkCR was developed for the bioreduction of 3,5-BTAP to (R)-3,5-BTPE, offered the best results in terms of high product concentration and productivity, demonstrating its great potential in industrial manufacturing of (R)-3,5-BTPE.

(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol [(R)-3,5-BTPE] is a valuable chiral intermediate for Aprepitant (Emend) and Fosaprepitant (Ivemend). Biocatalyzed asymmetric reduction is a preferred approach to synthesize highly optically active (R)-3,5-BTPE. However, the product concentration and productivity of reported (R)-3,5-BTPE synthetic processes remain unsatisfied.BACKGROUND(R)-[3,5-bis(trifluoromethyl)phenyl] ethanol [(R)-3,5-BTPE] is a valuable chiral intermediate for Aprepitant (Emend) and Fosaprepitant (Ivemend). Biocatalyzed asymmetric reduction is a preferred approach to synthesize highly optically active (R)-3,5-BTPE. However, the product concentration and productivity of reported (R)-3,5-BTPE synthetic processes remain unsatisfied.A NADPH-dependent carbonyl reductase from Lactobacillus kefir (LkCR) was discovered by genome mining for reduction of 3,5-bis(trifluoromethyl) acetophenone (3,5-BTAP) into (R)-3,5-BTPE with excellent enantioselectivity. In order to synthesize (R)-3,5-BTPE efficiently, LkCR was coexpressed with glucose dehydrogenase from Bacillus subtilis (BsGDH) for NADPH regeneration in Escherichia coli BL21 (DE3) cells, and the optimal recombinant strain produced 250.3 g/L (R)-3,5-BTPE with 99.9% ee but an unsatisfied productivity of 5.21 g/(L h). Then, four different linker peptides were used for the fusion expression of LkCR and BsGDH in E. coli to regulate catalytic efficiency of the enzymes and improved NADPH-recycling efficiency. Using the best strain (E. coli/pET-BsGDH-ER/K(10 nm)-LkCR), up to 297.3 g/L (R)-3,5-BTPE with enantiopurity >99.9% ee was produced via reduction of as much as 1.2 M of substrate with a 96.7% yield and productivity of 29.7 g/(L h).RESULTSA NADPH-dependent carbonyl reductase from Lactobacillus kefir (LkCR) was discovered by genome mining for reduction of 3,5-bis(trifluoromethyl) acetophenone (3,5-BTAP) into (R)-3,5-BTPE with excellent enantioselectivity. In order to synthesize (R)-3,5-BTPE efficiently, LkCR was coexpressed with glucose dehydrogenase from Bacillus subtilis (BsGDH) for NADPH regeneration in Escherichia coli BL21 (DE3) cells, and the optimal recombinant strain produced 250.3 g/L (R)-3,5-BTPE with 99.9% ee but an unsatisfied productivity of 5.21 g/(L h). Then, four different linker peptides were used for the fusion expression of LkCR and BsGDH in E. coli to regulate catalytic efficiency of the enzymes and improved NADPH-recycling efficiency. Using the best strain (E. coli/pET-BsGDH-ER/K(10 nm)-LkCR), up to 297.3 g/L (R)-3,5-BTPE with enantiopurity >99.9% ee was produced via reduction of as much as 1.2 M of substrate with a 96.7% yield and productivity of 29.7 g/(L h).Recombinant E. coli/pET-BsGDH-ER/K(10 nm)-LkCR was developed for the bioreduction of 3,5-BTAP to (R)-3,5-BTPE, offered the best results in terms of high product concentration and productivity, demonstrating its great potential in industrial manufacturing of (R)-3,5-BTPE.CONCLUSIONSRecombinant E. coli/pET-BsGDH-ER/K(10 nm)-LkCR was developed for the bioreduction of 3,5-BTAP to (R)-3,5-BTPE, offered the best results in terms of high product concentration and productivity, demonstrating its great potential in industrial manufacturing of (R)-3,5-BTPE.

A multiferroic material can simultaneously show two or more basic magnetic properties, including ferromagnetism, antiferromagnetism, and ferroelectricity. BiFeO is a multiferroic material with a rhombohedral distorted perovskite structure. Doping can reduce the volatility of Bi and greatly improve the magnetoelectric properties of BiFeO . To investigate the influence of the doping content we used the following analytical methods: X-ray powder diffraction (XRD), scanning electron microscopy (SEM), microwave network analysis (PNA-N5244A), and the Superconducting Quantum Interference Device (Quantum Design MPMS) test. With the increase of Ca concentration in the solution, the grain size of Bi Ca FeO becomes smaller, showing the role of Ca ions as the dopant for fine grains. The calcination temperatures are the major causes for the saturated magnetization. The residual magnetization ( M ) and the coercive force ( H ) decrease linearly with the increase of x value, and due to the effect of Ca substitution at Bi sites, which causes the valence change of Fe and/or the oxygen vacancies. The XRD result indicates that the diffraction peak emerges with the increase of Ca and the main diffraction peak achieves a high angle. The best calcining temperature is 600 °C, and the morphology is very dependent on the calcining temperature.

Unconventional protein secretion (UcPS) exports diverse signal peptide-lacking cargoes, yet its cargo selectivity remains poorly understood. Here, we identify TMED proteins as key regulators of vesicle-dependent UcPS, mediating selective cargo release via translocation into secretory carriers. TMED proteins act as translocators, facilitating cargo passage across lipid bilayers with assistance from HSP90 chaperones and partial cargo unfolding. Selectivity arises during translocation, where TMED cytoplasmic tails bind specific cargoes. The ER-Golgi intermediate compartment (ERGIC) is essential for TMED-mediated translocation and release. TMED homo-oligomerization enhances translocation, while hetero-tetramerization inhibits it. ERGIC localization promotes homo-oligomerization, which is further stabilized by cargo binding, forming a feed-forward mechanism to enhance translocation. These findings establish TMED proteins as central regulators of cargo diversity in UcPS, with their oligomerization and subcellular localization modulating translocation efficiency.