Explore the vast range of titles available.

Background Growing evidences indicate that the alterations in gut microbiota are associated with the efficacy of glucocorticoids (GCs) in the treatment of systemic lupus erythematosus (SLE). However, there is no evidence to prove whether gut microbiota directly mediates the effects of GCs. Methods Using the MRL/lpr mice, this study firstly addressed the effects of three doses of prednisone on gut microbiota. Then, this study used fecal microbiota transplantation (FMT) to transfer the gut microbiota of prednisone-treated MRL/lpr mice into the blank MRL/lpr mice to reveal whether the gut microbiota regulated by prednisone had similar therapeutic efficiency and side effects as prednisone. Results The effects of prednisone on gut microbiota were dose-dependent in the treatment of MRL/lpr mice. After transplantation into MRL/lpr mice, prednisone-regulated gut microbiota could alleviate lupus, which might be due to decreasing Ruminococcus and Alistipes and retaining the abundance of Lactobacillus . However, prednisone-regulated gut microbiota did not exhibit side effects as prednisone. The reason might be that the pathogens upregulated by prednisone could not survive in the MRL/lpr mice as exogenous microbiota, such as Parasutterella , Parabacteroides , and Escherichia-Shigella . Conclusions These data demonstrated that the transplantation of gut microbiota may be an effective method to obtain the therapeutic effects of GCs and avoid the side effects of GCs.

Autoimmune Bullous Diseases (AIBDs), characterized by the formation of blisters due to autoantibodies targeting structural proteins, pose significant therapeutic challenges. Current treatments, often involving glucocorticoids or traditional immunosuppressants, are limited by their non-specificity and side effects. Cytokines play a pivotal role in AIBDs pathogenesis by driving inflammation and immune responses. The JAK-STAT pathway is central to the biological effects of various type I and II cytokines, making it an attractive therapeutic target. Preliminary reports suggest that JAK inhibitors may be a promising approach in PV and BP, but further clinical validation is required. In AIBDs, particularly bullous pemphigoid (BP) and pemphigus vulgaris (PV), JAK inhibitors have shown promise in modulating pathogenic cytokine signaling. However, the safety and selectivity of JAK inhibitors remain critical considerations, with the potential for adverse effects and the need for tailored treatment strategies. This review explores the role of cytokines and the JAK-STAT pathway in BP and PV, evaluating the therapeutic potential and challenges associated with JAK inhibitors in managing these complex disorders.

The screen‐printing process of conductive ink can realize simple and large‐scale manufacture of micro/nano patterns for producing wearable electronic products. Herein, chitin nanocrystals (ChNCs) are used as a dispersant for the preparation of multiwalled carbon nanotube (MWCNT) ink with high viscosity and uniformity by ultrasound treatment. ChNCs can interact with MWCNT in noncovalent ways, including π–π and hydrophobic interactions. ChNCs/MWCNT (CCNT) ink does not aggregate even after standing for 3 months with a maximum MWCNT concentration of 33 mg mL−1 and dispersion efficiency of 91.1%. Using CCNT ink, a paper‐based thermoelectric generator (TEG) is manufactured by screen‐printing technology. With good thermoelectric and strain sensing properties, CCNT coated paper can stably collect human energy at room temperature to realize self‐powering. The CCNT coated paper‐based TEG can convert thermal voltage signals into musical notes, monitor the changes in human behavior and respiratory rate, and monitor joint movements. Moreover, CCNT coated paper has no cytotoxicity by CCK‐8 and live/dead staining. This work puts forward a strategy of green preparation of MWCNT‐based ink by adding renewable chitin, which opens up a new way to apply MWCNT‐based ink in self‐powering wearable multifunctional sensors. A stable and biocompatible multiwalled carbon nanotube ink stabilized by chitin nanocrystals is prepared, in which the two nanoparticles can interact via π–π and hydrophobic interactions. The conductive ink is coated on paper by screen‐printing technology to obtain the paper‐based thermoelectric generator, which shows multifunctional applications in self‐powering wearable sensor.

A simple and economic strategy to construct a chitosan-ink carbon nanoparticle sponge sensor was proposed by freeze-drying of chitosan and Chinese ink mixture solution. The microstructure and physical properties of the composite sponges with different ratios are characterized. The interfacial compatibility of chitosan and carbon nanoparticles in ink is satisfied, and the mechanical property and porosity of chitosan was increased by the incorporation of carbon nanoparticles. Due to excellent conductivity and good photothermal conversion effect of the carbon nanoparticles in ink, the constructed flexible sponge sensor has satisfactory strain and temperature sensing performance and high sensitivity (133.05 ms). In addition, these sensors can be successfully applied to monitor the large joint movement of the human body and the movement of muscle groups near the esophagus. Dual functionally integrated sponge sensors show great potential for strain and temperature detection in real time. The prepared chitosan-ink carbon nanoparticle composite shows promising applications in wearable smart sensors.

Simiao decoction, a classical traditional Chinese medicine (TCM) formula, has been widely used for thousands of years due to its safety and efficiency in treating gouty arthritis. Utilizing serum proinflammatory cytokines and gut ecosystems, this study elucidated the mechanisms of alleviating gouty arthritis by Simiao decoction. Simiao decoction (4.0, 8.0, and 16.0 g/kg) was orally administered to gouty arthritis mice and febuxostat was given as a positive control. The spleen, kidney, and liver indexes indicated that Simiao decoction was safe for the treatment of gouty arthritis in C57BL/6 mice. Besides, our study demonstrated that Simiao decoction was effective for reducing the level of serum uric acid and decreasing MPO, XOD, and ADA activity, as well as alleviating gouty-related symptoms, such as foot swelling and pain. Moreover, Simiao decoction could also reduce some specific serum proinflammatory cytokines including IL-1β, IL-9, IFN-γ, MIP-1α and MIP-1β. We then surveyed the effects of Simiao decoction on the gut ecosystems in a systematic manner by combining network pharmacology, ELISA, western blot, and illumina sequencing. In the murine of model of gouty arthritis, Simiao decoction could suppress NLRP3 inflammasomes expression, reduce gut apoptosis through modulating TNF-α, Caspase 8, and AIFM1 protein expressions, affect lipid metabolism by regulating APOB, LPL, PPARα protein expressions and restore gut microbiota via reducing potential pathogens. Overall, these findings suggested that Simiao decoction was an effective therapeutic drug for gouty arthritis and the gut ecosystem might act as a potential anti-inflammatory target of Simiao decoction.

The repair of skin injury has always been a concern in the medical field. As a kind of biopolymer material with a special network structure and function, collagen-based hydrogel has been widely used in the field of skin injury repair. In this paper, the current research and application status of primal hydrogels in the field of skin repair in recent years are comprehensively reviewed. Starting from the structure and properties of collagen, the preparation, structural properties, and application of collagen-based hydrogels in skin injury repair are emphatically described. Meanwhile, the influences of collagen types, preparation methods, and crosslinking methods on the structural properties of hydrogels are emphatically discussed. The future and development of collagen-based hydrogels are prospected, which is expected to provide reference for the research and application of collagen-based hydrogels for skin repair in the future.

is one of the predominant species in the human gut and exerts a series of beneficial effects. The aim of this study was to investigate the protective role of Bv46 in a dextran sodium sulfate (DSS) induced colitis mouse model. Female C57BL/6J mice were given 3% DSS in drinking water to induce colitis and simultaneously treated with Bv46 by gavage for 7 days. Daily weight and disease activity index (DAI) of mice were recorded, and the colon length and histological changes were evaluated. The effects of Bv46 on gut microbiota composition, fecal short chain fatty acids (SCFAs) concentration, transcriptome of colon, colonic cytokine level and cytokine secretion of RAW 264 7 macrophage cell line activated by the lipopolysaccharide (LPS) were assessed. Bv46 significantly attenuated symptoms of DSS-induced colitis in mice, including reduced DAI, prevented colon shortening, and alleviated colon histopathological damage. Bv46 modified the gut microbiota community of colitis mice and observably increased the abundance of , , and at the genus level. In addition, Bv46 treatment decreased the expression of colonic TNF-α, IL-1β and IL-6 in DSS-induced mouse colitis , reduced the secretion of TNF-α, IL-1β and IL-6 in macrophages stimulated by LPS , and downregulated the expression of and genes in mice colon, which mainly participate in the regulation of B cell responses. Furthermore, oral administration of Bv46 notably increased the contents of fecal SCFAs, especially butyric acid and propionic acid, which may contribute to the anti-inflammatory effect of Bv46. Supplementation with Bv46 serves as a promising strategy for the prevention of colitis.

Background Humidity was an unfavorable factor for patients with rheumatoid arthritis (RA). RA disease activity was severe in high humidity conditions. However, there is no evidence to demonstrate the effects of humidity on arthritis in the animal experiments and explore its relevant mechanism. Methods Using the DBA/1 mice, this study addressed the effects of a high humidity (80 ± 5%) on arthritis in collagen-induced arthritis (CIA) mice. Then, this study used the gas chromatography-mass spectrometer (GC-MS) to explore alterations in serum metabolome caused by the high humidity. Furthermore, xylitol and L-pyroglutamic acid, which were both significantly upregulated by the high humidity, were selected to further study their effects on arthritis in the CIA mice. Results The high humidity (80 ± 5%) could aggravate arthritis variables including increasing arthritis score and swelling, serum autoantibodies (anti-COII and anti-CCP), and proinflammatory cytokines (IL-6, IL-17A, and G-CSF). In addition, the high humidity could cause significant alterations in serum metabolome in the CIA mice. Xylitol and L-pyroglutamic acid were the representative serum metabolites that were significantly upregulated by the high humidity. Further experiments demonstrated that the supplementation of 0.4 mg/mL xylitol in drinking water after inducing the CIA model and 2.0 mg/mL in drinking water before inducing the CIA model could both aggravate arthritis in the CIA mice. Conclusions These data demonstrated that high humidity was not beneficial for arthritis development and its mechanism might be associated with xylitol and L-pyroglutamic acid.

Gliomas are the most common primary tumors of the central nervous system, with high heterogeneity and highly variable survival rates. Accurate classification and prognostic assessment are key to the selection of treatment strategies. One hallmark of the tumor is resistance to cell death. PANoptosis, a novel mode of programmed cell death, has been frequently reported to be involved in the innate immunity associated with pathogen infection and played an important role in cancers. However, the intrinsic association of PANoptosis with glioma requires deeper investigation. The genetics and expression of the 17 reported PANoptosome-related genes were analyzed in glioma. Based on these genes, patients were divided into two subtypes by consensus clustering analysis. After obtaining the differentially expressed genes between clusters, a prognostic model called PANopotic score was constructed after univariate Cox regression, LASSO regression, and multivariate Cox regression. The expression of the 5 genes included in the PANopotic score was also examined by qPCR in our cohort. The prognostic differences, clinical features, TME infiltration status, and immune characteristics between PANoptotic clusters and score groups were compared, some of which even extended to pan-cancer levels. Gene mutations, CNVs and altered gene expression of PANoptosome-related genes exist in gliomas. Two PANoptotic clusters were significantly different in prognosis, clinical features, immune characteristics, and mutation landscapes. The 5 genes included in the PANopotic score had significantly altered expression in glioma samples in our cohort. The high PANoptotic score group was inclined to show an unfavorable prognosis, lower tumor purity, worse molecular genetic signature, and distinct immune characteristics related to immunotherapy. The PANoptotic score was considered as an independent prognostic factor for glioma and showed superior prognostic assessment efficacy over several reported models. PANopotic score was included in the nomogram constructed for the potential clinical prognostic application. The associations of PANoptotic score with prognostic assessment and tumor immune characteristics were also reflected at the pan-cancer level. Molecular subtypes of glioma based on PANoptosome-related genes were proposed and PANoptotic score was constructed with different clinical characteristics of anti-tumor immunity. The potential intrinsic association between PANoptosis and glioma subtypes, prognosis, and immunotherapy was revealed.

Background Cotyledons serve dual roles as nutrient reserves and physical protectors during early seedling establishment. However, they are vulnerable to diverse granivorous and environmental stressors. Previous studies have predominantly focused on cotyledon loss magnitude, leaving a significant knowledge gap regarding how post-emergence timing of damage interacts with environmental factors such as nutrient availability to influence seedling establishment. Methods We conducted a controlled factorial experiment manipulating three cotyledon removal timings (1, 2, and 4 weeks post-emergence) and two nutrient supplementation levels (i.e., high and low nutrient level) treatment with a widely distributed oak species, Quercus dentata. Morphological (height, basal diameter, and biomass allocation, etc.) and physiological traits (photosynthetic rate, leaf nutrients, etc.) were measured after treatment for five months. Results Cotyledon removal induced severe and irreversible reductions in seedling height, basal stem diameter, and root biomass, showing that the first week after emergence is a critical vulnerability window. Nutrient supplementation exhibited time-dependent effectiveness. The compensatory effect became progressively weaker with increasingly earlier removal of cotyledons. High nutrient conditions significantly enhanced photosynthetic rates and leaf nutrient content across all timing removal treatments. Structural equation modeling revealed that nutrient influenced biomass primarily through indirect pathways, mediated by physiological traits rather than direct effects. Our model explained 90–94% of organ biomass variation. Conclusion Our findings demonstrate that cotyledon damage timing determines the success of Q. dentata seedling establishment. The identification of ontogenetic windows for vulnerability and compensatory capacity challenges the traditional view of cotyledons as static nutrient pools. If cotyledon removal is anticipated in Q. dentata restoration practices, implementing protective measures during the first post-emergence week and timing nutrient interventions to coincide with periods of maximum compensatory capacity could significantly enhance seedling establishment.