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From a global perspective, the stable isotope altitude effect is crucial for understanding climate information. However, the intensity of this effect can be influenced by the source of moisture, particularly in inland mountainous regions where the moisture sources are complex. Different combinations of moisture sources might affect the altitude effect. Focusing on the upper Shiyang River in the northern part of the Qilian Mountains in China, this study calculated the proportion of recycled moisture in precipitation and utilized the HYSPLIT model to determine the source of advective moisture. It explored the characteristics and mechanisms by which moisture sources affect the spatiotemporal variations in precipitation isotope effects within the study area. The findings indicated that: (a) The altitude effect follows a seasonal pattern: winter < autumn < spring < summer, with a reverse effect in winter. (b) As the contribution of recycled moisture to precipitation increases, the altitude effect of stable isotopes weakens, primarily due to the disruptive influence of recycled moisture on this effect. (c) The altitude effect of stable isotopes in precipitation is determined by the direction of the moisture source and its attributes. When the primary source of advective moisture runs perpendicular to the mountain range and the moisture migration speed is slow, the altitude effect is pronounced. Thus, although temperature directly causes the altitude effect, water vapor sources significantly influence it in inland mountainous regions. Key Points The altitude effect has significant seasonal variation, being strong in summer and weakest in winter The contribution of recirculating water vapor to precipitation is large, weakening the altitude effect The source of water vapor and the nature of the air masses contribute to the differences in elevation effects

A fundamental objective of genomics is to track variations in gene expression program. While metabolic RNA labeling-based single-cell RNA sequencing offers insights into temporal biological processes, its limited applicability only to in vitro models challenges the study of in vivo gene expression dynamics. Herein, we introduce Dyna-vivo-seq, a strategy that enables time-resolved dynamic transcription profiling in vivo at the single-cell level by examining new and old RNAs. The new RNAs can offer an additional dimension to reveal cellular heterogeneity. Leveraging new RNAs, we discern two distinct high and low metabolic labeling populations among proximal tubular (PT) cells. Furthermore, we identify 90 rapidly responding transcription factors during the acute kidney injury in female mice, highlighting that high metabolic labeling PT cells exhibit heightened susceptibility to injury. Dyna-vivo-seq provides a powerful tool for the characterization of dynamic transcriptome at the single-cell level in living organism and holds great promise for biomedical applications. A fundamental objective of genomics is to track variations in gene expression program. Here, authors developed Dyan-vivo-seq, which offers unprecedented insights into temporal RNA dynamics within intricate and dynamic biological systems at single-cell resolution.

Population-specific, positive selection promotes the diversity of populations and drives local adaptations in the population. However, little is known about population-specific, recent positive selection in the populations of cultivated cucumber (Cucumis sativus L.). Based on a genomic variation map of individuals worldwide, we implemented a Fisher’s combination method by combining 4 haplotype-based approaches: integrated haplotype score (iHS), number of segregating sites by length (nSL), cross-population extended haplotype homozygosity (XP-EHH), and Rsb. Overall, we detected 331, 2,147, and 3,772 population-specific, recent positive selective sites in the East Asian, Eurasian, and Xishuangbanna populations, respectively. Moreover, we found that these sites were related to processes for reproduction, response to abiotic and biotic stress, and regulation of developmental processes, indicating adaptations to their microenvironments. Meanwhile, the selective genes associated with traits of fruits were also observed, such as the gene related to the shorter fruit length in the Eurasian population and the gene controlling flesh thickness in the Xishuangbanna population. In addition, we noticed that soft sweeps were common in the East Asian and Xishuangbanna populations. Genes involved in hard or soft sweeps were related to developmental regulation and abiotic and biotic stress resistance. Our study offers a comprehensive candidate dataset of population-specific, selective signatures in cultivated cucumber populations. Our methods provide guidance for the analysis of population-specific, positive selection. These findings will help explore the biological mechanisms of adaptation and domestication of cucumber.

Gravity Recovery and Climate Experiment (GRACE) satellite data are widely used in drought studies. In this study, we quantified drought severity based on land terrestrial water storage (TWS) changes in GRACE data. We used the water storage deficit (WSD) and water storage deficit index (WSDI) to identify the drought events and evaluate the drought severity. The WSDI calculated by GRACE provides an effective assessment method when assessing the extent of drought over large areas under a lack of site data. The results show a total of 22 drought events in the central Asian dry zone during the study period. During spring and autumn, the droughts among these incidents occurred more frequently and severely. The longest and most severe drought occurred near the Caspian Sea. In the arid area of central Asia, the north of the region tended to be moist (the WSDI value was 0.04 year−1), and the south, east, and Caspian Sea area tended to be drier (the WSDI values were −0.07 year−1 in the south, −0.11 year−1 in the east, and −0.19 year−1 in the Caspian Sea). These study results can provide a key scientific basis for agricultural development, food security, and climate change response in the Asian arid zone.

Soil organic carbon is very important to increase crop yield. Understanding the composition changes and migration characteristics of SOC under different irrigation conditions in arid oasis areas is of great significance for the sustainable development of agro-ecosystem and the estimation of carbon balance of farmland ecosystem in arid areas. In this paper, classical statistics and geostatistics were used to study the leaching characteristics and migration laws of soil organic carbon under different irrigation conditions in Minqin Oasis, and the leaching amount and loss process and mechanism of SOC under different irrigation conditions were quantified. The research results showed that: (1) Irrigation increased the average 0–100 cm SOC content. After irrigation, SOC content decreased first and then increased with the increase in soil depth, with the maximum value of 8.56 g/kg and the minimum value of 1.82 g/kg. Compared with that before irrigation, SOC content in 0–30 cm surface layer was in leaching state, SOC content in 30–70 cm surface layer was accumulated, and SOC content in 70–100 cm surface layer had no obvious change. (2) Water was an important factor affecting soil carbon. The greater the irrigation amount, the higher the carbon leaching rate and the greater the migration amount. The SOC content in 0–30 cm soil layer was most significantly affected by irrigation, and the migration amount of SOC gradually decreased with the increase in soil depth. The maximum leaching rate of SOC was 36.8%, the minimum leaching rate was 13.5%, and its average leaching rate was 23.4%. (3) Due to the influence of soil infiltration rate under water, SOC content in different irrigation periods showed that the greatest SOC occurred in the 0–70 cm layer, and SOC content in soil was basically the same as that in non-irrigated areas on the fifth day after irrigation. (4) Precipitation, plastic film mulching, soil physical and chemical properties and other environmental factors were important factors affecting the migration and change in SOC content.

The Yellow River Basin is essential to China’s economic and social development and ecological security. In order to assess the temporal and spatial distribution of cloud water in the Yellow River Basin, we analyzed the distribution characteristics of water vapor content and cloud water content using ERA5 monthly averaged data from 1980 to 2019. The results showed that the high-value area of the annual average atmospheric water vapor content distribution was concentrated above the North China Plain in the eastern part of the basin, and the value was mostly between 21 and 24 mm. The low-value areas were mainly centered above the high mountain areas in the western part of the basin, and the value mostly fell between 3 and 6 mm. The seasonal distribution characteristics of the annual average water vapor content were relatively consistent with the annual average distribution characteristics. The high-value cloud ice water content area was in the northeastern part of the Qinghai-Tibet Plateau (4.5 g·m−2), while the low-value area appeared on the Loess Plateau (2 g·m−2). The high-value area of cloud liquid water content was on the north side of the West Qinling Mountains (12 g·m−2). The low-value area appeared on the Loess Plateau and the northern part of the Qinghai-Tibet Plateau (3 g·m−2). The cloud water content was higher in the eastern region than in the western region in the overall spatial distribution, and the content of cloud liquid water was higher than that of cloud ice water. The average annual atmospheric water vapor content was increasing, and the annual average content of cloud ice water and cloud liquid water was declining. The change in the total amount and spatial distribution of cloud water was not obvious in the Yellow River Basin.

Stable isotopes in precipitation can effectively reveal the process of atmospheric water circulation, serving as an effective tool for hydrological and water resource research, climate change, and ecosystem studies. The scarcity of stable isotope data in precipitation has hindered comprehension of the regional hydrology, climate, and ecology due to discontinuities on a temporal scale and unevenness on a spatial scale. To this end, we collated stable hydrogen and oxygen isotope data in precipitation from 842 stations in Eurasia from 1961 to 2022, totalling 51 752 data records. Stable isotopes in precipitation across various regions of Eurasia, as a whole, decrease with increasing latitude and distance from the coast. In the summer, stable isotopes in precipitation are relatively enriched, while in the winter, they are relatively depleted. In recent decades, the stable isotope values of Eurasian precipitation show an overall trend of increasing variation with the advancement of years, which is associated with global warming. Geographical location, underlying surface conditions, seasons, and atmospheric circulation are all factors that determine the characteristics of stable isotopes in precipitation. The dataset of stable isotopes in Eurasian precipitation provides a powerful tool for understanding changes in regional atmospheric water circulation and assists in conducting hydrological, meteorological, and ecological studies in related regions. The datasets are available at https://doi.org/10.17632/rbn35yrbd2.2 (Zhu, 2024).

Agricultural water accounts for more than 80% of the available water in arid areas. Agricultural activities have a great impact on surface water and groundwater. If the impact of agricultural activities on hydrochemistry is not prevented, the risk of water quality change in arid areas may be greatly intensified. Based on the hydrochemical data of the whole Shiyang River Basin from April 2014 to October 2019, this paper analyzes the impact of agricultural activities on hydrochemistry in the basin. The results show that (i) in the middle and lower reaches of farmland with high intensity of agricultural activities, the ion concentration of groundwater in summer and autumn is significantly higher than that in winter and spring due to the influence of irrigation; (ii) the runoff ion concentration in the backflow of the river reaches recharged by irrigation water is significantly higher than that of other reaches; (iii) due to strong evaporation, different types of reservoirs will lead to an overall increase in ion concentration, which is more obvious in plain reservoirs and river tail lakes. In addition, the reservoirs have a certain removal effect on nitrates.

T/myeloid mixed-phenotype acute leukemia (T/My MPAL) is a malignant disease characterized by co-expression of lymphoid and myeloid features. The lack of molecular classification of T/My MPAL results in highly heterogeneity in treatment responses and clinical outcomes. Identifying molecular subtypes and developing subtype-specific treatment strategies are crucial for improving prognosis and enabling personalized therapies. We constructed a single-cell transcriptomic landscape of T/My MPAL, acute myeloid leukemia (AML), T-cell acute lymphoid leukemia (T-ALL), and normal donors by analyzing nearly 275,000 cells. Malignant cells were identified using lineage-specific markers and healthy reference datasets. By comparing the whole transcriptomic profiles of T/My MPAL malignant cells with those of AML and T-ALL, we defined three distinct subpopulations and uncovered both intra- and inter-tumoral heterogeneity. Subpopulation-specific molecular markers were identified and validated using immunohistochemistry and independent datasets. These markers were further linked to clinical outcomes. Finally, potential subpopulation-specific therapeutic drugs were identified by correlating gene signatures with IC values. Malignant cells in T/My MPAL display distinct lineage characteristics and experience differentiation arrest at a more primitive stage compared to other leukemias. Biphenotypic and bilineal MPAL subtypes defined by flow cytometry exhibit similar transcriptomic profiles, indicating the traditional classification based on a limited set of lineage markers is insufficient. Instead, we define three subpopulations of malignant cells in T/My MPAL, including AML-like, T-ALL-like, and a unique subpopulation that shows distinct transcriptional characteristics neither similar to AML nor to ALL. Markers for each subpopulation are identified and further validated by independent datasets and immunohistochemistry. The unique subpopulation exhibits higher stemness and quiescence, with elevated HOPX expression. Notably, patients with higher levels of the unique subpopulation have significantly poorer prognoses. We further computationally screen potential drugs targeting each subpopulation and indicate that Venetoclax could effectively inhibit the unique MPAL subpopulation and help patient achieve complete remission. Our study provides new insights into the molecular heterogeneity and offers personalized diagnostic and therapeutic targets for T/My MPAL patients. These findings offer valuable insights for enhancing patient outcomes and developing personalized treatment strategies.

To evaluate the distribution characteristics of water vapor content (WVC), cloud liquid water content (CLWC) and cloud ice water content (CIWC) in arid areas of central Asia from 1980 to 2019 were analyzed by using average data of ERA5 in the European Centre for Medium-Range Weather Forecasts. The results show that: On the spatial scale, the WVC in the arid area of central Asia is mainly between 2 and 26 kg·m−2. The area of maximum water vapor content is distributed in southwestern Kazakhstan and southwestern Tajikistan, with a maximum value of 26 kg·m−2. The minimum areas are distributed in eastern Tajikistan, central Kyrgyzstan, central Tajikistan, and western Xinjiang, China, with the lowest WVC of 2 kg·m−2. The maximum of CLWC areas were mainly distributed in northwest Kazakhstan, with a maximum value of 0.08 kg·m−2, while the minimum areas were distributed in Tajikistan, eastern Kyrgyzstan, and northwest China, with a minimum value of 0.02 kg·m−2. The maximum areas of CIWC were distributed in the north of Tajikistan and the west of Kyrgyzstan, possessing a maximum value of 0.06 kg·m−2. The minimum area is distributed in the western part of Central Asia with a minimum value of 0.01 kg·m−2. From 1980 to 2019, the WVC generally increased, while the annual average CIWC and CLWC appeared a downward trend.