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The composite fuselage panel is a crucial component in large civil aircraft, necessitating higher detection rates and efficiency. Phased array ultrasonic testing can enhance the efficiency and defect detection rate; however, it is essential to consider the structural characteristics of the fuselage to ensure reliable identification of design defects. Based on the material properties, structural features, and defect detection requirements of composite fuselage panels, a test block reflecting their characteristics was designed and fabricated with various prefabricated defects. Ultrasonic phased array method was employed for a series of testing parameter optimization experiments and defect detectability analysis. The detected flaws were quantitatively analyzed and compared with those obtained using conventional ultrasonic automatic scanning techniques to validate the effectiveness of the developed ultrasonic phased array method. The feasibility of utilizing phased array ultrasonics for nondestructive evaluation of wide-body airliner composite fuselage panels is confirmed.

Post-translational modification of proteins is involved in the occurrence of endometriosis (EM); however, the role of ubiquitination modification in EM remains unclear. Integrin β3 (ITGB3) is one of the β-subunits of integrins, which plays a key role in tumor progression. In this study, we investigated the roles of ITGB3 and ITCH, one of the ubiquitin E3 ligases, in ectopic endometrial stromal cells (ESCs) and EM. Primary ectopic ESCs and normal ESCs were isolated and purified. Western blot was used to detect the expression of ITGB3 and ITCH in ESCs. The interaction between ITGB3 and ITCH in ESCs was investigated by the co-immunoprecipitation and ubiquitylation analysis. With or without the overexpression of ITCH and/or ITGB3, the proliferation and invasion of ectopic ESCs were detected by the CCK8 assay and transwell migration assay, respectively. We found that ITGB3 is upregulated in ectopic ESCs from patients with EM. ITCH interacts with ITGB3 by co-immunoprecipitation, and ITCH-overexpressing significantly increased the ubiquitination of ITGB3. The data of the CCK8 assays showed that ITGB3 overexpression significantly promoted cell proliferation of ectopic ESCs at 12, 24, 48, and 72 h. The transwell migration assays showed that ITGB3 overexpression significantly enhanced the invasive ability. However, ITCH had the opposite effects in both assays. Our findings indicate that ITCH-mediated ubiquitylation of ITGB3 regulates the proliferation and invasion ability of ectopic ESCs in EM.

Endometriosis (EMs) is a common disease among women whose pathogenesis is still unclear, although there are various hypotheses. Recent studies have considered macrophages the key part of the immune system in developing EMs, inducing inflammation, the growth and invasion of the ectopic endometrium, and angiogenesis. Extracellular vesicles (EVs) as novel intercellular vesicle traffic, can be secreted by many kinds of cells, including macrophages. By carrying long non-coding RNA (lncRNA), microRNA (miRNA), or other molecules, EVs can regulate the biological functions of macrophages in an autocrine and paracrine manner, including ectopic lesion growth, immune dysfunction, angiogenesis, and can further accelerate the progression of EMs. In this review, the interactions between macrophages and EVs for the pathogenesis of EMs are summarized. Notably, the regulatory pathways and molecular mechanisms of EVs secreted by macrophages during EMs are reviewed.

Vegetation effectively slows down the deterioration rate of the ecosystem in the water-level fluctuation zone (WLFZ). In this study, we investigated the impact of impoundment on the germination of seeds from Xanthium sibiricum fruits with various degrees of maturity and produced by X . sibiricum plants growing at different elevations in the WLFZ. Seed production by X . sibiricum under the current water level regulation mode was also investigated. Seeds were collected from seven elevations of the WLFZ of Three Gorges Reservoir (TGR) and exposed at these elevations to submergence. Seed production of the plants germinated from X . sibiricum seeds was observed. The maturity degree of X . sibiricum fruits from different elevations has no influence on the seed production by the plants that are produced from the fruits. Under the present water-level regulation mode, X . sibiricum growing above 155 m are able to generate mature seeds and thus provide seed sources for population maintenance, although the plants growing at an elevation below 165 m failed to produce fruits in 2010 due to unusual flooding. This study is useful for the vegetation recovery and reconstruction in other regions with hydrological characteristics similar to that of the TGR.

Phytoplankton functional groups have gained increasing attention in recent years. To understand the composition of phytoplankton functional groups in Poyang Lake and their drivers, field investigations were conducted over three years: 2013, 2014, and 2016. Phytoplankton were dominated by diatoms, contributing from 20.19% to 57.57% of the total biomass, followed by cyanobacteria, 9.81%–39.98%. Microcystis sp. and Anabaena sp. were the dominant species among cyanobacteria. Twenty-six functional groups were identified among 72 genera or species, and fiver groups (P, B, MP, H1, and G) were categorized dominant. All of dominant groups except H1 were tolerate highly fluctuating mixing intensities and showed a typical monthly succession variation. Water temperature, water level, and nutrients are key drivers for the variation of phytoplankton communities at taxonomic and functional levels. Both of redundancy and variation partitioning analyses showed that environmental variables might explain functional groups better than taxonomic communities, implying that the functional groups were more advantageous in showing the effects of environmental conditions than taxonomic compositions. Hydrological conditions have a crucial influence on phytoplankton assemblage dynamics in highly variable water regime lakes, but not in the largest ones. The functional groups method is suitable for identifying the spatial and seasonal characteristics of aquatic environments that significantly affected by water regimes.

Exosomes are key mediators of cellular communication by transporting molecules, including long noncoding RNAs (lncRNAs), and have been regarded as promising non-invasive biomarkers. This study aimed to evaluate the expression pattern and clinical significance of serum exosomal lncRNA antisense hypoxia inducible factor (aHIF) in epithelial ovarian cancer (EOC). Sixty-two EOC patients in Obstetrics and Gynecology Hospital of Fudan University were enrolled. The expression levels of aHIF in tissues and serum exosomes were examined by RT-qPCR. The origin of serum exosomal aHIF was explored in vitro and in vivo. Univariate and multivariate Cox regression analyses were used to evaluate the prognostic factors of EOC. A prognostic predictive nomogram was formulated in R software. We isolated exosomes, identified exosomal aHIF in the serum of EOC patients. The expression of serum exosomal aHIF was higher in EOC patients and was correlated with the aHIF level in EOC tissues. In vitro and in vivo, the results indicated that serum exosomal aHIF was derived from tumor cells. Kaplan-Meier survival analysis demonstrated that EOC patients with higher serum exosomal aHIF expression had poorer overall survival. Cox multivariate regression model revealed that FIGO stage, residual tumor size, and serum exosomal aHIF level were independent prognostic factors of EOC. Based on the prognostic value of serum exosomal aHIF, we established a nomogram model that showed a good predictive ability for EOC patients. Serum exosomal aHIF is overexpressed in EOC and can serve as a noninvasive predictive biomarker for unfavorable prognosis.

Global climate change has resulted in an increase in intensity and frequency of flooding, plants living in lowlands, and shore areas have to confront submergence caused by flooding, submergence-tolerant plants usually respond by adopting either escape or quiescence strategies. While certain plants exhibit a changeover from escape strategy upon partial submergence to quiescence strategy under complete shallow submergence, it remains unknown whether plants completely submerged at different water depths would adjust their strategies to cope with the change in submergence depth. Alternanthera philoxeroides is an ideal species to explore this adjustment as it is widely distributed in flood-disturbed habitats and exhibits an escape strategy when completely submerged in shallow waters. We investigated the responses of A. philoxeroides in terms of morphology, anatomy, and non-structural carbohydrate metabolism by conducting experiments using a series of submergence depths (0, 2, 5, and 9 m). During the submergence treatment, environmental factors such as light, dissolved oxygen, and temperature for submerged plants were kept constant. The results showed that A. philoxeroides plants submerged at depth of 2 m presented an escape strategy via fast stem elongation, extensive pith cavity development, and small biomass loss. However, the retarded stem elongation, reduced pith cavity transverse area, and increased biomass loss along the water depth gradient indicated that A. philoxeroides altered its growth response as water depth increased from 2 to 9 m. It is found that the changeover of response strategies occurred at higher submergence depths (5–9 m). Based on the results of our experiments, we demonstrated that water depth played an important role in driving the change in strategy. The water-depth-dependent growth performance of A. philoxeroides would benefit the species in habit exploration and exploitation. Further studies should focus on the performances of plants when submerged at varied water depths with different light climates and dissolved oxygen content, and how water depths drive the response behaviors of the submerged plants.

Background Cervical cancer is the most prevalent malignant tumor in women. This study aims to detect collagen type V α1 chain (COL5A1) expression and its clinical relevance in the prognosis of patients with cervical cancer. Methods Cervical cancer tissues and their paired adjacent normal tissues were prepared for tissue microarray. The expression of COL5A1 protein and the scores of the expression were evaluated by immunohistochemistry (IHC) staining. The prognostic value of COL5A1 was analyzed by R software version 4.2.1 with “survival, survminer, ggplot2” packages and Gene Expression Profiling Interactive Analysis (GEPIA). The cBioPortal database was utilized for the analysis of COL5A1 gene mutations. Results COL5A1 protein was overexpressed in human cervical cancer tissues compared to their paired adjacent normal tissues detected by IHC (P < 0.001). High expression of COL5A1 tends to be in elderly patients with cervical cancer. Survival analyses of clinical data of patients with cervical cancer showed that a high level of COL5A1 expression was significantly correlated with shorter overall survival (P = 0.031) and disease-free survival (P = 0.042) of patients. Further analyses of The Cancer Genome Atlas-Cervical Squamous Cell Carcinoma and the GEPIA survival datasets confirmed the association of high COL5A1 expression with poor overall survival of patients (P = 0.040 and P = 0.018, respectively). The analysis of genomic alterations of COL5A1 using the cBioPortal tool revealed that the COL5A1 gene was altered in 4% of cervical cancer patients and COL5A1 corresponding protein alterations with post-translational modifications were hydroxylation. Conclusion COL5A1 is a tissue biomarker correlated with the poor prognosis of patients with cervical cancer, which may lead to a new clinical application.

Background: Long noncoding RNAs (lncRNAs) are involved in the pathogenesis of endometriosis and can be regulated by estrogen. This study aimed to investigate the role of estrogen in regulating the expression and function of lncRNA-H19 in endometriosis. Methods: Endometrial stromal cells (ESCs) were isolated from ectopic, eutopic endometrium with endometriosis and control endometrium without endometriosis, and lncRNA-H19 expression was detected using real-time polymerase chain reaction (RT-PCR). Ectopic endometrial stromal cells (ecESCs) were treated with 17P-estradiol at 10-8mol/L for 0, 12, 24 and 48 hours, and lncRNA-H19 expressions of cells were evaluated using RT-PCR. After ecESCs were treated with 17P-estradiol for 48 hours, lncRNA-H19 expression was knocked down and cell proliferative and invasive abilities were compared. Results: The expression of lncRNA-H19 in ecESCs was significantly higher than that in eutopic endometrial stromal cells (euESCs) and control ESCs. After treated with 17P-estradiol, ecESCshadupregulatedlncRNA-H19 expression with time-dependent manner. Cell proliferation and invasion increased when estrogen upregulated lncRNA-H19 expression in ecESCs, however, cell proliferation restored and cell invasion did not change when lncRNA-H19 was knocked down in ecESCs. Conclusion: The expression and function of lncRNA-H19 was regulated by estrogen in ecESCs, which probably contributed to the pathogenesis of endometriosis. Keywords: endometriosis, lncRNA-H19, estrogen, proliferation, invasion, ectopic endometrial stromal cells

Intense precipitations caused by global climate change will result in the occurrence of greater frequencies and longer durations of flooding, influencing the survival and yields of wetland plants. Alisma orientale (Samuel.) Juz., an important traditional medicine with edible scape and inflorescence, naturally grows in wetlands and artificially cultivates in paddy fields prone to flood in China. However, we lack understanding of the effect of complete submergence on A. orientale. Here, experiments with four durations of complete submergence including 5 days (ds), 10 ds, 15 ds and 20 ds followed by 20 ds recovery were performed. In the submergence experiments, the number of, length of and biomass of surviving leaves and the total biomass and new blade biomass were measured; in recovery experiments, number and length of surviving leaves were measured. A. orientale grew out longer new leaves during complete submergence, with a dramatic decline in the biomass of both the leaves and tubers as well as the total biomass at the ends of the submergence experiments. The A. orientale plants had a high survival rate after submergence. The duration of submergence did not influence the time for A. orientale needed to start regrowing. At the end of recovery period, the submerged A. orientale plants generated more leaves, had more surviving leaves, had shorter new leaves and a shorter total length of surviving leaves than the control plants. This study highlights that A. orientale plants can resist at least 20 ds of complete submergence caused by flooding and regrow rapidly after submergence and improves our understanding of the flooding tolerance mechanisms of A. orientale plants.