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"Liu, Yi Song"
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Spatial Modelling of Urban Wind Characteristics: Review of Contributions to Sustainable Urban Development
Wind, a renewable resource with growing importance in the contemporary world, is considered a capable tool for addressing some of the problems linked with rapid urbanization, unsustainable development, and climate change. As such, understanding modelling approaches to wind characteristics in cities becomes crucial. While prior reviews delve into the advancements in reduced-scale models and computational fluid dynamics simulations, there is scant literature evaluating large-scale spatial modelling of urban wind environments. This paper aims to consolidate the understanding of spatial modelling approaches to wind characteristics in cities by conducting a systematic literature review with the PRISMA protocol to capture the contributions to sustainable urban development. The reviewed articles are categorized under two distinctive approaches: (a) studies adopting the wind morphometric approach, encompassing theoretical foundations, input factors, and computation methods and (b) studies adopting the urban climate mapping approach, centering on the amalgamation of wind with urban microclimate analysis. The findings suggest that wind morphometric methodologies hold considerable promise due to their straightforward calculations and interpretability. Nonetheless, issues related to data precision and accuracy challenge the validity of these models. This review also probes into the implications of these two distinctive approaches for urban planning and policymaking, advocating for more sustainable urban development.
Journal Article
PPM1B degradation mediated by TRIM25 ubiquitination modulates cell cycle and promotes gastric cancer growth
Protein phosphatase PPM1B, a member of the serine/threonine phosphatase family, has been implicated in various human cancers. In this study, our objective was to investigate the role of PPM1B in GC growth and explore the underlying mechanisms. Our findings revealed that PPM1B expression was downregulated in GC tissues, and higher levels of PPM1B expression were associated with improved overall survival in GC patients. Overexpression of PPM1B significantly inhibited cell proliferation, induced G1 phase cell cycle arrest, and suppressed tumor growth. Conversely, knockdown or knockout of PPM1B yielded opposite effects. Mechanistically, we identified that PPM1B exerted its inhibitory role in GC cell growth and cell cycle regulation through the TRIM25/PPM1B/CDK2 signaling pathway. Specifically, we demonstrated that TRIM25 physically interacts with PPM1B, leading to enhanced degradation of PPM1B and subsequent modulation of CDK2 phosphorylation and GC cell growth. PPM1B emerges as a potential prognostic biomarker and therapeutic target in GC. These findings hold clinical significance by offering opportunities to improve diagnosis and treatment strategies for GC patients. Furthermore, this study provides novel insights into the pathogenesis and progression of GC, expanding our understanding of this disease.
Journal Article
Research on the Efficacy of Ganpu Vine Tea in Inhibiting Uric Acid Production
Ganpu vine tea is a new type of health care citrus fruit tea made from citrus shell, Pu-er tea, and vine tea baked as raw materials. In this study, the in vitro uric acid synthase inhibition system and hyperuric acid cell model were constructed to appraise the uric acid lowering efficacy of Ganpu vine tea, traditional Ganpu tea, and vine tea. Results showed that in the uric acid synthase inhibition system, the aqueous extract can inhibite the puric metabolically related enzymes, such as adenosine deaminase (ADA), purine nucleoside phosphorylase (PNP), and xanthine oxidase (XOD). The ability of the aqueous extract to inhibit the above enzyme was as follows: vine tea > Ganpu vine tea > Ganpu tea; all teas had a strong effect on XOD inhibition. The hyperuric acid cell model test showed that the aqueous extract inhibited uric acid production through accumulating inosine and hypoxanthine and hindering xanthine synthesis. The uric acid reductive ability was as follows: Vine tea > Ganpu vine tea > Ganpu tea. The inhibition of enzymes related to uric acid synthesis and the inhibition of uric acid production were significantly enhanced through adding vine tea to Ganpu tea. It also shows that flavonoids are the main factor driving this ability because they are the main active ingredients in these botanical drinks.
Journal Article
Metabolism and Tissue Distribution of Chelerythrine and Effects of Macleaya Cordata Extracts on Liver NAD(P)H Quinone Oxidoreductase
Background: Macleaya cordata (Willd.) (Papaveraceae) is listed as a feed additive in animal production by the European Food Authority. Methods: The metabolites of chelerythrine in rats were measured in vitro and in vivo by rapid and accurate high-performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (HPLC/QqTOF-MS). The structures of CHE metabolites were elucidated by comparing their changes in accurate molecular masses and fragment ions with those of parent ion or metabolite. The metabolic enzymes that were involved in chelerythrine reduction were investigated using an inhibition method. The tissue distribution of chelerythrine and the effects on NQO1 following intragastric administration with M. cordata extracts in rats were examined. Results: A total of twelve metabolites of chelerythrine were characterized by this approach in rat liver S9 and in vivo . The reduction of the iminium bond of chelerythrine and subsequent O-demethylation was the main metabolic pathway of chelerythrine in rat liver S9 while the reduction of the iminium bond of chelerythrine was the main metabolic pathway of chelerythrine in rats in vivo . After the rats were given intragastric administration, the low concentration residues of sanguinarine and chelerythrine in different rat tissues were found at 48 h after the last dose, suggesting that both compounds could be widely distributed in tissues. The results also indicated that XO, NQO1, NQO2, and carbonyl reductase are involved in chelerythrine reduction. Macleaya cordata extracts treated female and male rats, respectively, showed different responses, inhibiting NQO1 activity in males, but inducing NQO1 activity in females. Chelerythrine had a weak impact on NQO1 activity, but sanguinarine inhibited NQO1 activity Conclusion: Through studying the effects of cytosolic reductase inhibitors on chelerythrine reduction and the impact of chelerythrine and sanguinarine on the activity of NQO1 in vitro and in vivo , we clarified the potential drug interaction of Macleaya cordata extract in clinical application, so as to provide theoretical guidance for clinically safe medication. In addition, it provided a reference basis for the metabolic mechanism of chelerythrinein rats.
Journal Article
Molecular Characterization and Phylogenetic Analysis of Spirometra Tapeworms from Snakes in Hunan Province
Sparganosis is a neglected zoonotic parasitic disease that poses huge threats to humans worldwide. Snakes play an important role in sparganosis transmission because they are the most common second intermediate hosts for Spirometra parasites. However, the population genetics of Spirometra isolates from snakes is currently not well studied in China. The present study was performed to explore the molecular characteristics and phylogenetic analysis of Spirometra tapeworms from different species of snakes in Hunan Province. This study obtained 49 Spirometra isolates from 15 geographical areas in Hunan Province, Central China. Subsequently, the 18S and 28S ribosomal DNA (rDNA) fragments were amplified from the isolated parasites, and their sequences were analyzed to assess their genetic diversity. Phylogenetic analyses were performed using the maximum likelihood algorithm. The results showed that sequence variations among these isolates were 0–2.3% and 0–0.1% for 18S and 28S rDNA, respectively. The phylogenetic analysis showed that all Spirometra isolates from Hunan Province were clustered into the same branch with Spirometra erinaceieuropaei isolated from other areas (China, Vietnam, Australia). Moreover, the phylogenetic trees revealed that Spirometra is closely related to Adenocephalus, Pyramicocephalus, Ligula, Dibothriocephalus, Schistocephalus, and Diphyllobothrium. The Spirometra isolates of different hosts/regions in Hunan Province are not host segregated or geographically isolated, and support for the taxonomic status of Spirometra tapeworms in China has been added. These results provide reference values for future accurate identification and taxonomic status of Spirometra tapeworms in China.
Journal Article
Proteomics analysis of faecal proteins in the tick Haemaphysalis flava
Background
Ticks and tick-borne diseases are of major public health concern. Currently, development of vaccines against ticks is considered crucial for their control. A critical step in this process is the screening of viable antigens. Faeces are byproducts of digestion and blood meal utilization, and partly reflect the vitality and vector potential of ticks. However, an integrated analysis of proteins in tick faeces is lacking. The present study explored the protein components in the faeces of the tick
Haemaphysalis flava
, by liquid chromatography–tandem mass spectrometry (LC/MS-MS) to identify potential protein antigens for vaccine development against ticks.
Methods
Faeces from adult
H. flava
engorged females were collected. Proteins were extracted from faeces, and the trypsin-digested peptides were analyzed by LC/MS-MS. High confidence proteins were identified based on unique peptides revealed by MS. Potential faecal protein genes, as well as their sources, were also characterized by searching previous transcriptome datasets from the salivary glands and midgut of
H. flava
.
Results
In total, 21 were recognized with confidence. Amongst these, 18 were of likely tick origin, while three proteins (serum albumin, haemoglobin α and β subunits) were likely from hosts. Seventeen unigenes corresponding to these proteins were retrieved by searching our previous
H. flava
salivary glands and midgut transcriptomic datasets. Some proteins were reported to prevent blood clotting, play a role in immunity and antibiosis, and formation of musculature
.
The functions of the remaining proteins are unknown.
Conclusions
Identifying antigens for tick vaccine development is feasible by analyzing the faecal proteome as well as the transcriptomes of salivary glands and midguts. The vast number of proteins detected in tick faeces highlights the complexity of blood digestion in ticks, a field that needs more investigation.
Journal Article
Leveraging the Opportunities of Wind for Cities through Urban Planning and Design: A PRISMA Review
Wind has been utilized for passive ventilation and mechanical power since antiquity. As an abundant renewable resource, today, wind is increasingly seen as a critical resource to help tackle issues associated with rapid urbanization and climate adaptation and mitigation, such as improving thermal comfort, providing clean energy, improving air quality, and reducing carbon emissions. Despite the growing importance of wind as an invaluable resource for cities, wind in the context of urban planning and design is a relatively understudied area of research. This study aims to explore the means by which cities that can benefit from wind and ways urban planning and design can help deliver these benefits. The study adopts a systematic literature review methodological approach. The findings disclosed that: (a) improving urban wind environment via sound urban planning and design may enhance urban ventilation and energy performance; (b) better urban ventilation and energy performance enable cities to become climate positive or net zero and relieve the urgent climate crisis; (c) wind sensitive urban design is an emerging research area critical to harvest the benefits of wind for cities. This study offers a novel conceptual framework and research directions for wind sensitive urban design and informs urban planning, design policy and practices.
Journal Article
MicroRNA-145-5p attenuates high glucose-induced apoptosis by targeting the Notch signaling pathway in podocytes
MicroRNAs (miRNAs/miRs) are considered to serve essential roles in podocyte apoptosis, and to be critical in the development of diabetic nephropathy (DN). Activation of the Notch signaling pathway has been demonstrated to serve an important role in DN development; however, its regulatory mechanisms are not fully understood. The present study used a high glucose (HG)-induced in vitro apoptosis model using mouse podocytes. Expression levels of miR-145-5p and its target, Notch1, and other key factors involved in the apoptosis signaling pathway were detected and measured by reverse transcription-quantitative PCR and western blotting. A luciferase reporter assay was performed to elucidate the miRNA-target interactions. The functions of miR-145-5p in apoptosis were detected using flow cytometry and TUNEL staining. The present study demonstrated that in HG conditions, miR-145-5p overexpression inhibited Notch1, Notch intracellular domain, Hes1 and Hey1 expression at the mRNA and protein levels. Notch1 was identified as a direct target of miR-145-5p. Furthermore, cleaved caspase-3, Bcl-2 and Bax levels were reduced significantly by miR-145-5p overexpression. These results indicate that miR-145-5p overexpression inhibited the Notch signaling pathway and podocyte lesions induced by HG. In conclusion, the results of the present study suggested that miR-145-5p may be a regulator of DN. Additionally, miR-145-5p inhibited HG-induced apoptosis by directly targeting Notch1 and dysregulating apoptotic factors, including cleaved caspase-3, Bcl-2 and Bax. The results of the present study provided evidence that miR-145-5p may offer a novel approach for the treatment of DN.
Journal Article
Flow Stealer： lightweight load balancing by stealing flows in distributed SDN controllers
Software-defined networking （SDN） introduces centralized controllers to increase network programmability drastically. Distributed control planes, in which multiple SDN controllers work together to manage a network, have been proposed to satisfy the requirements of large-scale networks, and different kinds of load-balancing approaches have been proposed to balance the workloads among these controllers. Current load-balancing approaches generally use switch migration, which adjusts the mapping between switches and con- trollers dynamically according to controller workloads. These switch migration-based approaches face challenges under burst traffic as a result of their overhead and longer detection periods. This paper proposes Flow Stealer, a lightweight load-balancing method for distributed SDN controllers. Flow Stealer uses a low-cost flow-stealing method, in which idle controllers share workloads temporarily with overloaded controllers by stealing flow events from them. The flow-stealing method not only can react to changes of network traffic more quickly, but can also reduce the frequency of switch migration. In addition, Flow Stealer incorporates both flow stealing and switch migration to adapt to burst traffic and long-term traffic changes. Experimental results show that Flow Stealer can balance the workloads among controllers more efficiently, especially under burst traffic.
Journal Article